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Copyright of Journal of Mechanical & Electrical Engineering is the property of Mechanical & Electrical Engineering Magazine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Research of Soil & Water Conservation is the property of Institute of Soil & Water Conservation, Chinese Academy of Sciences & Ministry of Water Resources and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Pathophysiology is the property of Jinan University, School of Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Advances in Earth Science (1001-8166) is the property of Advances in Earth Science Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Preventive Veterinary Medicine / Zhongguo Yufang Shouyi Xuebao is the property of Chinese Journal of Preventive Veterinary Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Human subpatellar fat pad is a kind of waste, which is often removed in total knee arthroplasty. If it is fully utilized, it can be used as a source of mesenchymal stem cells. OBJECTIVE: To explore the methods of isolation, culture and identification of human infrapatellar fat pad derived stem cells in vitro, to induce chondrogenic differentiation under specific conditions, and to explore the feasibility of being used as seed cells for cartilage tissue engineering. METHODS: The discarded infrapatellar fat pad from eight patients undergoing the total knee arthroplasty was obtained and human infrapatellar fat pad derived stem cells were isolated and cultured according to trypsin digestion, type I collagenase digestion and differential adherence methods. After being cultured to the third generation in vitro, cell morphology observation, cell growth curve and cell surface antigen analysis were performed. In addition, chondrogenic differentiation was induced, and human infrapatellar fat pad derived stem cells were identified by immunohistochemistry, toluidine blue staining and RT-qPCR. RESULTS AND CONCLUSION: (1) Isolated, purified and cultured human infrapatellar fat pad derived stem cells showed spindle-shaped adherent growth. The growth curve of the third generation of human infrapatellar fat pad derived stem cells was in the shape of "S". Cells were positive for CD44 and CD105, while negative for CD45. (2) After 14 days of induction, immunohistochemical staining and toluidine blue staining were positive in the differentiation group, and the relative gene expression levels of ACAN, BMP-2, COL2A1 and SOX-9 were significantly higher than those in the blank control group. (3) This study confirmed that mesenchymal stem cells with differentiation potential could be isolated from infrapatellar fat pad, which had strong proliferation ability and chondrogenic differentiation ability in vitro, and were expected to become ideal seed cells for cartilage tissue engineering. [ABSTRACT FROM AUTHOR]

Copyright of Acta Agronomica Sinica is the property of Crop Science Society of China and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Bohai University (Philosophy & Social Science Edition) / Bohai Daxue Xuebao (Zhexue Shehuikexue Ban) is the property of Bohai Daxue and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Autophagy can alleviate the development of knee osteoarthritis by inhibiting cell apoptosis. However, it is still unclear that whether miR-140 can regulate the key gene uncoordinated 51 like kinase-1 (ULK1) in autophagy. OBJECTIVE: To explore the regulatory role of miR-140 on the ULK1 gene. METHODS: The microRNA website was used to predict whether the ULK1 was the target gene of miR-140. Firstly, the full-length sequence of 3'-untranslated region of ULK1 was embed in the downstream of the renilla luciferase gene. Secondly, the mutant sequence of binding sites was constructed to obtain wild type vector and mutant vector. Thirdly, the expression activity of luciferase was detected after transfecting 293T cells with these luciferase vectors. Lastly, 293T cells were treated with miRNA-140 inhibitor, and the expression level of ULK1 protein was detected by western blot assay. RESULTS AND CONCLUSION: The expression activity of luciferase was significantly decreased in 293T cells transfected by wild type vector and miR-140 mimics compared with the cells transfected by wild type vector alone (P < 0.01). In addition, the expression of ULK1 protein was significantly increased when miR-140 was inhibited (P < 0.01). These results imply that miR-140 can regulate the expression of ULK1 gene by binding with its gene sequence. [ABSTRACT FROM AUTHOR]

OBJECTIVE:To probe into relevant factors and regularities of adverse drug reactions (ADR)/ adverse drug events (ADE) induced by general characteristics, so as to provide references for promoting clinical rational drug application. METHODS:301 cases of ADR/ADE reports in National Center for ADR Monitoring from Jan. 1st 2006 to Apr. 30th 2014 were collected and retrospectively analyzed in terms of patients` ages, genders, diseases, routes of administration, drug combinations, occurrence time, species of antibiotics, organs and (or) systems involved and their clinical features, severe ADR/ADE involved drugs, etc. RESULTS:Of 301 reports on ADR/ADE induced by antibiotics, the number of female was slightly more than male; mostly aged over 60 years(122 cases, accounted for 40.53%); mostly respiratory disease (139 cases, accounted for 46.18%); intravenous drip was the main route of administration inducing ADR/ADE; most ADR/ADE were induced by drug combination. Cephalosporins was the No.1 antibiotics inducing ADR/ADE, secondly were fluoroquinolones and penicillin; the top 3 drugs were respectively cefoperazone tazobactam, levofloxacin and ciprofloxacin. The main organs and (or) systems involved by ADR was skin and its accessories, secondly were systemic damage and digestive system damage. Of 301 reports of ADR/ADE, 24 cases (accounted for 7.97%) were severe ADR/ADE, mostly were induced by cephalosporins. CONCLUSIONS:The occurrence of ADR/ADE is related to many factors, doctor-medicine-patients cooperation should be reinforced to develop the function of clinical pharmacist, so as to promote rational drug application. [ABSTRACT FROM AUTHOR]