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Asthenozoospermia, a condition observed in 40% of infertile males, is characterized by reshiced sperm mastility. MicroRNAs (miRNAs) play a pivotal role in spermatogenesis, yet their involvement in asthenozoospermia remains poorly understood. This study aims to elucidate the molecular mechanisms of miRNAs in asthenospermia. Method (): Sperm samples wer collected from individuals with severe asthenozoospermia and healthy males. High-throughput sequencing was employed to identify differentially expressed miRNAs., followed by bioinformatics analysis of these significant miRNAs. The ahered expression of two specific miRNAs and their target genes was confirmed using qRT-PCR. Rasult(): When comparing severe asthenozoospermia patients to healthy males, 146 miRNAs exhibited significam alterations (0.05; log2 Fold Change>1), with 52 upregulated and 94 downregulated miRNAs. The ten most significantly upregidated and downregulated miRNAs were subjected to target gene prediction through the miRDB and TargetScan databases, a total of 1407 target genes were found to be supported by both databases. Enrichment analysis revealed that miRNA target genes were enriched in sperm cell processes, and they wem involved in oxidative metabolism, stimadus response, proliferation, differentiation, and apoptosis in sperm cells. Pathway analysis indicated that target genes might participate in processes such as autophagy, cellular senescence, the PIK-Akt signaling pathway, the MAPK signaling pathway, the HIF-1 signaling pathway, and the mTOR signaling pathway Notably, ansing the specifically upwegıdated miRNAs in ast henazoospermia, haa-miR-371-5p-and haa-miR-2355-5p were identified, with their respective target genes being the autophagy effector protein Berlin! and matochondrial inner membrane protein prohibain2 (PHB2), both directly involved in mutochondrial autophagy. qRT-PCR results demonstrated an increused expression of haa-mik-371a-5p and hsa-miR-2355-5p in sperm as motility declined. Conclusion(s): This study identified miRNAs with specific dysregulation in the sperm of asthenozoospermia patients and their target genes, offering new insights and a theoretical basis for further investigation into the mechanisms by which miRNAs regulate mitochondrial autophagic functions inlow motility sperm. [ABSTRACT FROM AUTHOR]

Objective To explore the characteristics and diagnostic significance of ultrasound signs in the diagnosis of acute appendicitis in children. Methods This study focused on 81 children with acute appendicitis and divided them into two groups based on pathological examination results: 34 children with severe progressive appendicitis (41.98%) and 47 children with simple appendicitis (58.02%). By analyzing the indirect and direct signs of ultrasound detection, as well as pathological examination data, and using ROC curve analysis to analyze the area under the curve ( area under curve, AUC) of ultrasound signs combined, a comprehensive analysis is conducted to score the ultrasound examination results of children. Results The detection rates of wall continuity interruption/low-level clarity, intraluminal fluid accumulation, periappendiceal or abdominal fluid accumulation, periappendiceal hyperechogenicity, cecal and ileal wall thickening in the advanced group were higher than those in the simple group (P < 0.05); The scores of indirect, direct, and combined ultrasound signs in the progressive group were higher than those in the simple group (P < 0.05); Under the ROC curve, the sensitivity, specificity, positive predictive value, and negative predictive value of combined signs were 98.77%, 97.53%, 98.77%, and 96.30%, respectively, higher than those of indirect signs and direct signs. The AUC was 0.835, higher than those of indirect signs and direct signs ( P < 0.05) . Conclusion The combined diagnosis of ultrasound examination signs can provide objective evidence for the early diagnosis of acute appendicitis in children, and can also achieve dynamic monitoring of the disease, which is conducive to the formulation of clinical treatment plans. [ABSTRACT FROM AUTHOR]

【Objective】The effect of exogenous methyl jasmonate on resistance to bacterial leaf stripe (BLS) was studied, which would provide a theoretical basis for the prevention and control of BLS in rice.【Method】With rice BLSresistant and susceptible near isogenic lines (K9 and G9) as materials, 0.1 mmol/L methyl jasmonate (MeJA) were sprayed on BLS-resistant and susceptible near isogenic lines (K9 and G9) plants respectively at tillering stage, and water spraying was used as control (CK). The inoculation with Xanthomonas oryzae pv. Oryzicola (Xoc) were conducted at 0, 12, 24, 48, 72 h after MeJA treatment, then the symptoms of BLS were investigated. In K9 and G9 plants sprayed with MeJA for 48 h then inoculated with Xoc, the expression changes of hormone-pathway related genes were further analyzed at 0, 6, 12, 24 and 48 h after inoculation.【Result】In K9 plants, the lesion lengths of leaves inoculated at 0, 24, 48, 72h after being sprayed with MeJA were significantly shorter than those in CK; in G9 plants, the lesion lengths of leaves inoculated at 0 h and 12 h after being sprayed with MeJA were significantly longer than those in CK, while inoculated at 72 h after being sprayed with MeJA were significantly shorter than those in CK. Compared with the CK, relative expression levels of jasmonate-pathway related genes LOX2 and AOS2, and pathogenesis-related gene PR10 were upregulated, while relative expression levels of alicylic acidpathway related genes PAD4 and PAL, ethylene-pathway related genes EIN2 and ERF70, and pathogenesis-related gene PR1a were downregulated in K9 plants inoculated at 48h after being sprayed with MeJA; relative expression levels of jasmonatepathway related genes LOX2 and AOS2, salicylic acid-pathway related genes PAD4 and PAL and pathogenesis-related genes PR1a and PR10 were upregulated, while relative expression level of ethylene-pathway related gene EIN2 was downregulated in G9 plants inoculated at 48h after being sprayed with MeJA.【Conclusion】Exogenous MeJA affects the BLS-resistance in rice resistant and susceptible near isogenic lines. The resistance responses of resistant and susceptible near isogenic lines to Xoc are different at different inoculation time points after MeJA treatment. Exogenous MeJA involves in the defense response to Xoc infection by inducing the expression changes of jasmonate, salicylic acid and ethylene pathway-related genes and pathogenesisrelated genes. [ABSTRACT FROM AUTHOR]

Objective To evaluate the reliability and validity of the Chinese version of the modified dental ;mxiety scale (CMDJ\ S),and to provide a th eoretical basis for its application in China. Methods J\ t

The experiment was to explore the effect of corn straw fermented by different strains on growth performance, serum biochemical index, immune function and meat quality of Siemental cattle. 100 healthy Simental bulls with similar weight were selected and randomly divided into four groups, with five replicates in each group and five cattle in each replicate. The Siemental cattle in control group were fed with basic diet + no bacteria corn straw. The Siemental cattle in group 1, group 2 and group 3 were fed with basic diet + 5% corn straw fermented by wood mold, yeast and lactic acid bacteria, respectively. The pre-test period was 5 d, and the formal test lasted for 60 d. The results showed that the terminal weight of Siementhal cattle in test group 3 was significantly higher than that in other groups (P<0.05), and the feed to gain ratio of Siementhal cattle in test group 3 was significantly lower than that in other groups (P<0.05). The total protein and glucose content in all the test groups were significantly higher than those in control group (P<0.05), and the test group 3 had the highest total protein and glucose content. The IgG and IgA content of all the test groups were higher than those in control group with no significant difference (P>0.05), and the test group 3 had the highest IgG and IgA content. The a* value and crude protein content of beef in all the test groups were significantly higher than those in control group (P<0.05), and the test group 3 had the highest a* value and crude protein content. The experiment indicates that adding 5% fermented corn straw to the diet of Simental cattle can promote the growth performance of Simontal cattle, increase the content of total protein and glucose in the blood, and improve immune function and meat quality. The corn straw fermented with lactic acid bacteria has the best effect. [ABSTRACT FROM AUTHOR]

Objective To systematically review the prognostic value of the pre-treatment prognostic nutritional index (PNI) for patients with breast cancer.Methods The PubMed,Cochrane library,Embase,Web of science,Wangfang data,VIP,CNKI and other electronic databases were searched systematically,and including relevant literature.Two researchers independently screened the literature,extracted valid data and evaluated the quality.For PNI and other clinical parameters,overall survival (OS),disease-free survival (DFS) correlation research,as well as PNI and breast cancer patients clinical pathological characteristics of the correlation research,conduct a meta-analysis.Results 4 articles were included,with a total of 1 165 patients.Compared with the low pre-treatment PNI group,OS and DFS of the high PNI group were significantly prolonged, and the differences were statistically significant (hazard ratio=0.42,0.64,P =0.000 2,0.02).There was no significant correlation between low pre-treatment PNI and age,tumor stage,histological type,recurrence,and neoadjuvant chemotherapy (P >0.05).Conclusion A low pre-treatment PNI was significantly associated with poor prognosis in patients with breast cancer,and it has no obvious correlation with clinical parameters such as age,tumor stage,histological type,etc.A high pre-treatment PNI is indicated better OS and DFS in patients [ABSTRACT FROM AUTHOR]

胄癌是一种临床常见恶性肿瘤，其发病率高、死亡率高， 晚期患者预后欠佳。近年来，人们对于免疫冶疗的研究越 来越多，免疫治疗在临床治疗中也取得了可喜的效果。程序性死亡受体(PD 1)及程序性死亡受体配体(PD L1)免疫靶点治疗能 起到免疫杀伤肿瘤细胞的作用， 已被用于包括胄癌在内的多种肿疮。多项研究证实， PD 1/PD L1 抑制剂在胄癌治疗中具有效果 良好， 不良反应可控的优点， 为目前有前途的冶疗药物。该文主要就PD 1 /PD L1 信号通路及PD 1 /PD L1 免疫治疗在胃癌冶 疗中的研究进展进行综述。 [ABSTRACT FROM AUTHOR]

In order to improve the efficiency of cargo flow distribution in inland container transportation network and determine the planning suggestions for improving the operation level of regional transportation network, a robust optimization model of cargo flow distribution based on situational regret value was established under the background of uncertain freight demand, taking inland container transportation network in northeast China as the research object. On the basis of the optimization scheme of cargo flow allocation, the clustering of alternative hub nodes is analyzed comprehensively by community detection, and the capacity constraint of critical path is released for sensitivity analysis. The results show that the method is effective and applicable, which can provide a scientific basis for the planning and construction of inland container transportation network. [ABSTRACT FROM AUTHOR]

Atherosclerosis is the main pathological basis of coronary heart disease.It is a complicated progress involved many factors,and the result of the interaction between genetic and environmental factors.In addition to factors such as age, gender, obesity,smoking,hypertension and diabetes,abnormal lipid metabolism is a particularly important factor in promoting the development of atherosclerosis.Nowadays,there is increasing evidence that non-coding RNA is involved in the regulation process and plays an important role in the lipid metabolism and formation of atherosclerosis.Especially,the RNA interference (RNAi) using small interfering RNA (siRNA) has achieved significant lipid-lowering effects not only in animal experiments,but also in clinical trials.This article reviewed influencing factors of lipid metabolism and related interventions on non-coding RNA level. [ABSTRACT FROM AUTHOR]

Objective To compare the effects of renal denervation (RD) and drug therapy on cardiac function» renin-angiotensin-aldosterone system* sympathetic nervous system and cardiac autonomic nervous system in rats with post-acute myocardial infarction (AMI) heart failure and to explore the appropriate time for RD treatment and it's therapeutic mechanism. Methods One hundred and forty Wistar rats were randomly divided into control group. AMI group. RD group. MI-ld + RD group. Ml-ld + Drugs group. MI-4w + RD group and MI-4w + Drugs group (20 in each group). RD and drug therapy (^-blocker combined angiotensin converting enzyme inhibitors) was applied one day and 4 weeks after AMI separately. The cardiac function, brain natriuretic peptide (BNP). plasma renin-angiotensin 11 • aldosterone ( ALD). norepinephrine ( NE) • infarcted myocardium norepinephrine content* heart rate (HR) and standard deviation of NN interval within 5 minutes (SDNN) were measured and recorded in each group 8 weeks post AMI. Results On the eighth week after AMI. left ventricular ejection fraction (LVEF) in control group. AMI group. RD group. Mi-Id + RD group. Ml-ld + Drugs group. MI- 4w+ RD group and MI-4w + Drugs group was 85%, 36%, 82%, 77%, 65%, 53%, 45 % respectively. Compared with the control group. AMI group showed a significant decrease in LVEF. infarcted myocardium NE content and HR ( P . (15). Compared with MI and MI-Id+ Drugs groups. LVEF in MI-id + RD group increased significantly (P<0. 05) .while the plasma BNP.renin.angiotensin 11 . ALD and NE decreased significantly (P<0. 05). Compared with AMI group. Mi-id + RD group and MI-id + Drugs group showed a significant increase in infarcted myocardium NE content and 11R ( P< (1.(15).and a significant decrease in SDNN (P<0. (15).while MI-4w+ RD group and MI-4w + Drugs group showed no significant difference in NE-HR and SDNN (PXJ.05). Conclusions The efficacy of early RD intervention in improving cardiac function after AMI is better than that of combination therapy of beta-blocker and angiotensin converting enzyme inhibitors. Its therapeutic mechanisms include inhibition of the sympathetic nervous system, renin-angiotensin-aldosterone system activity, and regulation of cardiac sympathetic-vagal balance. [ABSTRACT FROM AUTHOR]