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Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To study the effects of mirNA-21 (Mir-21)on the biological characteristics and whether miRNA-21 can regulate angiogenesis in HER-2 positive breast cancer. Methods 40 breast cancer tissues were collected. The expression of mir-21 was detected by real-time PCR. Ability of proliferation was carried out by CCK8 assay and the ability of invasion was studied by transwell invasion assay. The expression of VEGF was researched by western blotting assay. Results Mir-21 showed higher expression in breast cancer tissue than in the adjacent noncancer tissue (P<0.001). Overexpression of miR-21 will not influence the prognosis (P = 0.47 for all breast cancer patients and P = 0.26 for HER-2 positive breast cancer patients). Inhibiting the expression of miR-21 inhibited the proliferation (P<0.01)and invasion (P<0.001)of HER-2 positive breast cancer cell, it also inhibited the expression of VEGF. Conclusion The oncogene miR-21 plays significant role in HER-2 positive breast cancer biological behaviour and angiogenesis [ABSTRACT FROM AUTHOR]

Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To study the way of establishing patient-derived lung cancer xenograft models and discovering the biological characteristics of xenografts. Methods NSCLC tissue were collected from 4 primary lung cancer patients and implanted in subcutaneous layer of the nude mice. The growth condition and groph of tumors were observed and recorded，and the histopathology，immunohistochemistry and real-time PCR were used to confirm the graft tumors were or not derived from the patients. Results Two out of four xenograft models were successfully established. HE staining and immunohistochemistry analysis confirmed that major histological features of the original tumors were retained in xenografts. The expression of Ki67 and mitochondria were evaluated by qPCR，results revealed that xenografts had a tight correlation with primary patient tumors. Conclusions The subcutaneous layer xenograft approach was a feasible way to establish xenograft models derived from patient tumor. The xenografts recapitulate the genotype and phenotype of original patient tumors. [ABSTRACT FROM AUTHOR]