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Objective To study factors influencing the outcome of immune thrombocytopenia (ITP) in children, and construct a risk prediction model. Methods A total of 166 children with ITP were included in this study. Clinical characteristics and laboratory indicators of children with different outcomes were comparatively analyzed. Multivariate Logistic regression analysis was performed to screen factors influencing the outcome of children with ITP, and a risk prediction model was constructed based on analysis results. Hosmer-Lemeshow tested the goodness of fit of the model. The receiver operating characteristic (ROC) curve was plotted to analyze the predictive performance of the model. Results There were 118 children with good outcomes and 48 children with poor outcomes. Compared with the good outcome group, the proportions of age ≥1, ANA positive at first diagnosis and blood urea nitrogen (BUN) level were higher in the poor outcome group, while the proportion of platelet count (PLT) ≥20´109/L at first diagnosis, absolute lymphocyte count (ALC) at first diagnosis and the percentage of megakaryocytes in bone marrow at first diagnosis were lower in the poor outcome group (P＜0.05). Multivariate Logistic regression analysis showed that age ≥1 (95% CI: 1.358-83.563) and higher BUN level (95% CI: 4.013-32.615) were risk factors for disease outcome in children with ITP. PLT ≥20´109/L at initial diagnosis (95% CI: 0.036-0.519) and higher ALC at initial diagnosis (95% CI: 0.197-0.571) were protective factors for disease outcome in children with ITP. Homser-lemeshow test showed c2=8.486, P=0.387. The AUC of this model in predicting the outcome of children with ITP was 0.963 (95% CI: 0.934-0.991, P＜0.001), and the sensitivity and specificity were 95.83% and 88.98%. Conclusion Age ≥1 and higher BUN level are risk factors for disease outcome in children with ITP. PLT ≥20´109/L at initial diagnosis and higher ALC level at initial diagnosis are protective factors. The risk prediction model constructed according to these influencing factors has good predictive valuse for disease outcome of children with ITP. [ABSTRACT FROM AUTHOR]

Objective To investigate the prognostic role of EHD3 and its association with immune cell infiltration in gastric cancer. Methods In this study, EHD3 expression was analyzed using RNA sequencing data from Cancer Genome Atlas (TCGA). Differential analysis, functional enrichment, immune infiltration, immune checkpoint exploration, and clinical baseline data analysis were performed. Independent prognostic factors were identified using univariate and multivariate Cox regression analysis; a column diagram model was developed and evaluated using C-index and calibration diagrams. Results EHD3 was significantly upregulated in STAD and functional enrichment analysis showed that EHD3 expression was associated with immune response, with most immune cells and immune checkpoints positively correlated with their expression. Cox regression showed that EHD3 was an independent prognostic factor in STAD patients (HR=2.112, 95% CI: 1.340-3.327, P=0.001). Conclusion EHD3 is considered to be a novel prognostic biomarker for STAD patients, and this study provides a potential therapeutic target for STAD treatment. [ABSTRACT FROM AUTHOR]

[Objectives] Haemophilus parasuis, a common commensal Gram-negative bacterium in the upper respiratory tract of swine, can cause Glässer's disease under stress conditions. The objective of the experiment was to explore the interactions between H. parasuis and host. [Methods] Swine macrophage cells (3D4/21) were infected with standard serovar 5 of H. parasuis (Hps5), and RNA-Seq was used to survey the expression of long noncoding RNA (IncRNA) and mRNA. [Results] There were 2 654 IncRNA identified and which were comprised of 2 439 intergenic IncRNA (lincRNA) and 159 anti-sense IncRNA. Compared with uninfected cells, 110 and 216 IncRNA were up-and down-regulated, respectively. The up-regulation patterns for 10 IncRNA identified by RNA-seq were analyzed in 3D4/21 cells infected by Hps5 using real-time PCR. Four IncRNA were consistently up-regulated at 12, 24 and 36 h. Especially, the up-regulation of IncRNA 165 and 3304 were associated with the phagocytosis of living bacteria into cells. However, we didn't reveal the relationship between the expression of IncRNA 165,3304 and the strains virulence. The RNAi and over-expression of IncRNA 165 showed that this could affect the phagocytosis of H. parasuis by modulating the expression of TGF-β1. [Conclusions] IncRNA 165 played an important role during the uptake of H. parasuis by 3D4/21 cells through modulation of TCF-β. [ABSTRACT FROM AUTHOR]

Objective. To investigate the suicidal ideation among unmarried female miworkers in Shanghai and Guangzhou and explore the association between suicidal ideation and some psychosocial problems, reproductive health factors. Methods. A questionnaire survey was conducted among 3 553 unmarried female miworkers in two factories in Shanghai and two in Guangzhouconvenient sampling. Logistic regression was used to analyze the related factors of suicide ideation including psychosocial problems and reproductive health factors. Results. Overall, 287(8.1%) of the respondents had suicidal ideation during the past year. Univariate Logistic regression showed that alcohol, low self-esteem, depression, anxiety, loneliness, poor social support (emotional support and economic support), favorable attitude toward premarital pregnancy, favorable attitude toward multiple induced abortion, experience of unplanned pregnancy and experience of induced abortion were related to suicidal ideation after demographic factors being corrected. Multivariate Logistic regression showed that alcohol (OR1.42, 95% CI 1.04-1.93), low self-esteem (OR1.47, 95% CI 1.03-2.11), loneliness (OR3.30, 95% CI 2.42-4.51), poor emotional support (OR2.68, 95% CI 2.00-3.61) and poor economic support (OR3.79, 95% CI 2.86-5.04) were the critical factors. Conclusion. The factors such as risk behavior, mental health, lack of social support were associated with increased odds for suicidal ideation among the unmarried female miworkers. The prevention and intervention mechanism should be constructed, and the social support should be enhanced to effectively prevent the occurrence of suicidal ideation. [ABSTRACT FROM AUTHOR]

Objectives To evaluate the periodontal health status of women of childbearing age in small area of Kunming city by investigating the periodontal diseases of pregnant women and non-pregnant. Methods 213 pregnant women and 255 non-pregnant women aged from 25 to 35 years old were randomly enrolled in a case-control study. Periodontal status was assessed through periodontal examinations. Results Of the pregnant women， 91.0% were detected with debris，83.5% with calculus，the incidence of gingivitis was 98.0%，and 48% suffered from moderate or severe inflammation. DI-S in this group scored 1.44， CI-S scored 1.34， while GI scored 1.5， suggesting gingivitis in pregnant women was prevailing moderately. Of the non-pregnant women， 90.4% were detected with debris， 80.9% with calculus， the incidence of gingivitis was 42.0% ， and 8.5% suffered from moderate or severe inflammation. DI-S in this group scored 1.41， CI-S scored 1.21， while GI scored 0.68， suggesting gingivitis in non-pregnant women was prevailing mildly. No significant differences were observed in the incidence of calculus and debris， the incidence of gingivalis in pregnant women was significant higher than non-pregnant women （ ＜0.05）．Conclusions The periodontal status of childbearing age women in Kunming city is not fair， and pregnant women are more vulnerable to periodontal disease. In order to improve maternal periodontal health of pregnant women in Yunnan Province，we need enhancing oral hygiene instruction. [ABSTRACT FROM AUTHOR]