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Objective: To investigate the value of ultrasonic shear wave elastography (SWE) combined with serum adiponectin (AFN) and urokinase plasminogen activator (uPA) in differentiating benign and malignant thyroid nodules. Methods: 127 patients with thyroid nodules who underwent surgical thyroidectomy were selected in our hospital from January 2020 to December 2022. All patients underwent SWE examination before surgery, serum AFN and uPA levels were detected, and postoperative pathological results were recorded. According to the postoperative pathological results, the patients were divided into malignant group and benign group. Analyzed the value of SWE parameters combined with serum AFN and uPA in differentiating benign and malignant thyroid nodules. Results: There were 21 cases of malignant nodules (malignant group) and 106 cases of benign nodules (benign group). In the malignant group, the serum uPA level was higher than that in the benign group (P<0.05), and the serum AFN level was lower than that in the benign group (P<0.05). The area under the curve of Max, Mean, Min, AFN and uPA were 0.609, 0.754, 0.600, 0.777 and 0.701, respectively, and the area under the curve of combined Max, Mean, Min, AFN and uPA was 0.935, higher than each indicator to identify separately. Conclusion: In patients with malignant thyroid nodules, SWE parameters Max, Mean, Min and serum uPA levels increased, while AFN levels decreased. Combining SWE parameters with serum AFN and uPA can improve the differential efficacy of thyroid nodules. [ABSTRACT FROM AUTHOR]

Objective: To investigate the influencing factors of fear of cancer recurrence (FCR) after hepatic arterial chemoembolization (TACE) surgery in patients with primary liver cancer (PHC), and to observe the therapeutic effect of cognitive behavioral therapy in patients with FCR. Methods: 400 patients with PHC after TACE surgery who were admitted to Renji Hospital Affiliated to Medical College of Shanghai Jiaotong University from September 2020 to April 2022 were selected as research objects. General data of all patients were obtained according to the self-made scale. FCR of cancer patients was evaluated by Fear of Progression Questionnaire-Short Form (FoP-Q-SF), and the influencing factors of FCR occurs were analyzed by univariate and multivariate Logistic regression. Patients with FCR were divided into control group (n=128) and study group (n=128) according to random number table method. The control group received routine intervention, and the study group received cognitive behavioral therapy intervention on the basis of the control group, and the therapeutic effect of cognitive behavioral therapy in patients with FCR was observed. Results: The FoP-Q-SF score of 400 patients with PHC after TACE surgery was (38.43±5.29) scores, included 256 cases with total score greater than or equal to 34 scores and 144 cases with total score less than 34 scores. Univariate analysis showed that FCR of PHC patients after TACE is related to the per capita monthly income, gender, clinicopathological stage, age, postoperative time, marital status, TACE treatment times, disease coping style, educational level and social support (P<0.05). Multivariate Logistic regression analysis showed that risk factors for FCR in PHC patients after TACE include≥10 times of TACE treatment, low age, negative coping style of disease, educational level of primary school and below, low social support, per capita monthly income of family<3000 yuan, and clinical pathological stage III (P<0.05). After intervention, the scores of FoP-Q-SF, Self-rating Anxiety Scale (SAS) and Self-rating Depression Scale (SDS) were decreased in the two groups, and the study group was lower than the control group(P<0.05). Conclusion: Lower age, primary school education or below, TACE treatment times greater than or equal to 10 times, negative coping style, low social support, per capita monthly family income less than 3000 yuan, clinicopathological stage III are the risk factors for FCR in patients with PHC after TACE surgery. [ABSTRACT FROM AUTHOR]

ABSTRACT Objective: To investigate the diagnostic value of abdominal ultrasound combined with serum alpha fetoprotein (AFP), Golgi protein 73 (GP73), γ- glutamyl transpeptidase/platelet ratio (GPR) in patients with chronic hepatitis B liver fibrosis. Methods: A total of 148 patients with chronic hepatitis B who were admitted to Xinjiang Uygur Autonomous Region Hospital of Traditional Chinese Medicine from March 2020 to February 2022 were selected, and they were divided into non-liver fibrosis group with 39 cases, mild liver fibrosis group with 33 cases, moderate liver fibrosis group with 51 cases and severe liver fibrosis group with 25 cases according to the pathological diagnosis of liver biopsy. The results of abdominal ultrasound examination and serum AFP, GP73 and GPR were compared between the groups after admission. The receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of abdominal ultrasound combined with AFP, GP73 and GPR in patients with chronic hepatitis B liver fibrosis. Results: With the increase of the degree of liver fibrosis, the contour of the liver was gradually unclear, the surface was not smooth, the echo of the liver was gradually thickened and enhanced, there was a sense of nodulation, the shape of the intrahepatic bile duct was gradually blurred, the proportion of fatty liver and asastic fluid was gradually increased, the thickness of spleen, the length of spleen, the inner diameter of portal vein and the inner diameter of splenic vein were gradually increased. One-way analysis of variance showed that there were significant differences in ultrasound indicators among groups(P<0.05). The sensitivity of abdominal ultrasound in the diagnosis of patients with chronic hepatitis B liver fibrosis was 79.82%, the specificity was 76.92%, the accuracy was 79.05%. With the increase of the degree of liver fibrosis, serum AFP, GP73 and GPR increased gradually, and the differences were statistically significant (P<0.05). ROC curve analysis showed that scrum AFP, GP73 and GPR had higher area under curve (AUC) for the diagnosis of patients with chronic hepatitis B, which were 0.701, 0.664 and 0.779, respectively. The AUC of abdominal ultrasound combined with serum AFP, GP73 and GPR for the diagnosis of patients with chronic hepatitis B liver fibrosis was the highest (AUC was 0.819). Serum AFP, GP73 and GPR are increased in patients with chronic hepatitis B liver fibrosis. Abdominal ultrasound combined with serum AFP, GP73 and GPR has high diagnostic value for patients with chronic hepatitis B liver fibrosis. [ABSTRACT FROM AUTHOR]

To observe the effects of resistance training combined with Baduanjin on cardiac function, quality of life and adverse cardiac events in patients with chronic heart failure (CHF). 120 patients with CHF who were treated d in Internal Medicine-Cardiovascular Department of the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine from June 2019 to July 2020 were included, they were randomly divided into control group and study group by random number table method, with 60 cases in each group. Both groups were treated with routine anti heart failure treatment, on this basis, the control group received resistance training, and the study group received resistance training combined with Baduanjin. Both groups were intervened for 6 months. The curative effects, cardiac function and quality of life of the two groups before and 6 months after intervention were compared, and the occurrence of adverse cardiac events during follow-up were recorded. The total clinical effective rate of the study group was higher than that of the control group (P<0.05). 6 months after intervention, the left ventricular end systolic diameter (LVESD) and left ventricular end diastolic diameter (LVEDD) of the study group were smaller than those of the control group, and the left ventricular ejection fraction (LVEF) was larger than that of the control group (P<0.05). 6 months after intervention, the score of SF-36 all dimensions (physical health, mental health, general health, emotional function, physical function, social function, physical pain, energy) of the study group was larger than that in the control group (P<0.05). The incidence of adverse cardiac events of the study group was lower than that of the control group (P<0.05). Resistance training combined with Baduanjin can effectively improve the cardiac function, improve the quality of life and reduce the incidence of adverse cardiac events in patients with CHF. [ABSTRACT FROM AUTHOR]

Objective To explore the effects of different stages injection of botulinum toxin type A (BTXA) on hypertrophic scars in rabbit ear models. To determine the optimal time for BTXA to inhibit scar. Methods From September 2018 to March 2019, 18 healthy New Zealand white rabbits were selected to establish hypertrophic scar models. According to the time of BTXA injection, all rabbits were divided into the immediate group (group A), the 10-day group (group B) and the 3-week group (group C). Each group was subdivided into the injection groups (group A1, B1 and C1) and control groups (group A2, B2 and C2) according to the injection of BTXA. The samples were collected at 30 days after injection. In the control groups before injection (group A0, B0 and C0), the samples were collected before injection. A total of 18 rabbits with 36 ears were prepared for hypertrophic scar models, 4 scar models were established on each ear. There were 144 scar models, 16 models in each subgroup (2 rabbits). The BTXA (40 U/ml) was injected at the 3 o'clock and 9 o'clock location of the wounds, 2 U per point. The growth of scar of wound was observed and recorded. The thickness of scar was measured and the numbers of fibroblasts and blood vessels were calculated by HE staining. The distribution and arrangement of collagen fibers was observed by Masson staining. Results The increase of scar thickness in group A1, B1 and C1 was lower than that in group A2, B2 and C2, respectively. The difference in scar thickness between the injection group and the control group in group A and B was greater than that in group C. The difference was statistically significant ( X²=19.486, P<0.05). The growth of fibroblasts in group A1 was less than that in group A2, and the decrease of fibroblasts in group B1 was greater than that in group B2. The difference in the number of fibroblasts between the injection group and the control group was largest in group B, followed by group A, and smallest in group C. There was statistically significant difference ( X²=30.535, P<0.05). The amount of vascular growth in group A1 was less than that in group A2, and the amount of vascular reduction in group C1 was greater than that in group C2. The difference in the number of blood vessels between the injection group and the control group in group A was greater than that in group B and C. The difference was statistically significant (F=22.889, P<0.05). Compared with group A2, B2 and C2, less collagen fiber proliferation and relatively orderly fiber bundles were observed in group A1, B1 and C1. Conclu原 sion Immediate, 10-day and 3-week injection of BTXA after injury could inhibit hypertrophic scars in rabbit ears. Notably, the immediate injection was the best choice. BTXA could inhibit the fibroblasts and collagen fibers. The decreased number of blood vessels may be one of the mechanisms by which BTXA inhibits scarring. [ABSTRACT FROM AUTHOR]

Ensuring the security of strategic minerals is related to the overall situation of national modernization. In the context of the new era, the process of economic globalization continues to accelerate, and the inherent characteristics of high integration and interdependence of the world economy are gradually emerging; on the other hand, the supply and demand pattern of the global mining market is constantly adjusting, and 'black swan' incidents continue to emerge, bringing many challenges to mineral resource security. In addition, the sustainable development strategy puts forward higher requirements for ecological environmental protection. It is of important research value to ensure the security of minerals to realize the scientific and rational development and utilization of strategic mineral resources, to emphasize the coordinated development of resource development and environmental protection, and to insist on open development to promote global mining win-win cooperation for national security and green development. This article expounds the concept of strategic mineral resource security in the new era, and discusses the construction of its evaluation index framework by using cobalt to test its adaptiveness. The results show that; (1) The concept of China's strategic mineral resource security in the new era is aimed at national resource security interests and the principle of 'excellent coexistence', for the purpose of coordinating China's national security and that of the rest of the world, and taking into consideration the dual synergy of China's national will and its market power to achieve the sustainable and coordinated development of global environmental governance and domestic society and economy. (2) The index framework from three dimensions, namely global resource supply stability, domesticly resource economic security, and superior coexistence, can be effectively used to evaluate the security of China's strategic mineral resources in the new era. (3) Cobalt security index in China and the coexistence index show the same trend during the study period, indicating that the improvement of the integration of domestic and foreign cobalt resource markets will improve China's security of cobalt resources. [ABSTRACT FROM AUTHOR]

Objective　To improve laboratory research of generic drugs in Chinese pharmaceutical enterprises, avoid the defects in the research process, and explore the feasibility of six sigma management (DMAIC) as improvement model in the process of generic drugs laboratory research. Methods　The effectiveness of DMAIC implementation was evaluated through the literature search and case study of a generic API’s process with the DMAIC model. Results　The DMAIC model was successfully applied in this case. The model made laboratory research more reasonable, helped R&D personnel to pinpoint key quality attributes and process parameters, etc., ensured the authenticity and completeness of experimental data, and provided valuable data for subsequent industrial production as well as meeting registration requirements. Conclusion　DMAIC model can solve the problems, such as poor overall arrangement, poor connection with production, and irregular management. It embodied the idea of Quality by Design (QbD) and can be used as good reference for domestic laboratory research of generic drugs. [ABSTRACT FROM AUTHOR]

：目的 总结2015年1月～2018年12月山东省济南市肿瘤患者血型不规则抗体分布特点，为保证输血安全提供参考。 方法 回顾性分析本单位供血医院送检的117例不规则抗体筛查阳性肿瘤患者的血型不规则抗体检出情况，按不同血型系统、肿瘤发病部位、性别及免疫史对检出的不规则抗体进行统计。 结果 济南市血型不规则抗体主要分布于Rh、MNSs和Lewis血型系统。 36例泌尿生殖系统肿瘤患者中检出抗－E 13例(36.1%）、抗－M7例(19.4%);35 例消化系统肿瘤患者中，检出抗－E 10例(28.6%）、抗－M 10例(28.6%);13例循环系统肿瘤患者中，检出抗－E4例，抗－D2例；10例呼吸系统肿瘤患者中，检出抗－M4例，抗－E 3例；8例神经系统肿瘤患者中，检出抗－M4例；6例内分泌系统肿瘤患者中，检出抗－E4例、抗－M2例。 78例女性肿瘤患者中检出抗－E28例(35.9%）、抗－M 14例(21.8%),39例男性肿瘤患者中检出抗－M 15例(38.4%），抗－E 12例(30.1%) ;77例有免疫史（妊娠或输血史）女性患者中，检出抗－E27例(35.1%）、抗－M 13例(16.9%）；有输血史的男性肿瘤患者中检出抗－E9例。结论 济南地区肿瘤患者血型不规则抗体主要集中于Rh及MNSs血型系统；不同类型肿瘤患者中检出前两位的不规则抗体是抗－E和抗－M抗体；有免疫史（妊娠史或输血史）的女性和男性肿瘤患者中最常见的不规则抗体是抗－髻E抗体。 [ABSTRACT FROM AUTHOR]

The relationship and genetic diversity were studied with ISSR, RAPD and RAMP. With ISSR, RAPD and RAMP, the proportions of polymorphism were 84. 1 %， 86. 2 % and 92. 6 %， respectively and the average primer generated polymorphic bands were 8. 6, 8. 3 and 14. 1 respectively. Integrated three tag clustering results showed that the 32 radish germplasm in genetic distance 0. 30 was in clearly divided into two groups, the first group consists of 31 species, are all from domestic or sister varieties, and the second group of black radish MTHS a lone from Europe. Clustering results based on the ISSR, RAPD and RAMP markers combination data, are in high accordance with their own origins root color and the shape. There was some difference between three integrated clustering analysis and the marking alone, comprehensive analysis of the results more intuitive reflect the genetic relationship of materials between figure and consistency of horticulture trait is higher than any single mariner. [ABSTRACT FROM AUTHOR]

As conservation education has become the core mission of contemporary zoological parks, zoos in China have gradually taken it as their important duty. In the recent five years, there is a lack of international research on the overall Asian zoo conservation education activities, so as the domestic studies, but a condensed focus on the western cases. Thus, this study selects 295 conservation education activities of 34 zoos and aquariums of WAZA Asian members as research samples, analyze their education objectives, contents, methods and project animals, and some representative online educational materials are explored at the Covid-19 background. The result showed that zoo conservation education can inspire future professionals in the field of wildlife protection, establish a connection between popular science and scientific research, explain the relationship between protection and development, and arouse people's attention to native species and the local ecological environment. Therefore, the social significance of conservation education in zoos needs to be re-examined. [ABSTRACT FROM AUTHOR]

Container-based microservice deployment is a challenging problem that aims to improve user experience and increase cloud providers’ profitability by reducing microservice failure rates and response times while maximizing resource utilization. It presents an enhanced accelerated particle swarm optimization algorithm to tackle the multiobjective optimization problem of microservice container deployment in a cluster. By considering the invocation relationships between microservices, the algorithm facilitates the aggregation of containers, thereby reducing data transmission costs, lowering failure rates, and enhancing cluster resource utilization. Experimental results demonstrate that the proposed optimization algorithm yields significant improvements in performance measures including data transmission overhead, failure rate, and resource utilization when compared to existing deployment algorithms.Specifically, the algorithm achieves a container aggregation improvement exceeding 40%, an average increase in data transmission consumption of over 4%, a decrease in failure rate by 10% to 20%, and an increase in utilization rate by roughly 3%. The aforementioned findings attest to the efficacy of the proposed algorithm. [ABSTRACT FROM AUTHOR]

目的　探讨血必净联合乌司他丁静脉注射对ＡＲＤＳ患者支气管肺泡灌洗液中相关细胞因子的影响。方法　 将１００例ＡＲＤＳ患者随机分为Ａ、Ｂ、Ｃ、Ｄ组各２５例，均予对症支持治疗；在此基础上Ｂ组加乌司他丁静脉注射，Ｃ组加血 必净静滴治疗，Ｄ组加血必净静滴与乌司他丁联合静脉注射治疗，疗程均７ｄ。各组分别于治疗前及治疗第４、７天采用双 抗ＥＬＩＳＡ法检测支气管肺泡灌洗液中ＴＮＦ-α、ＩＬ-６、ＩＬ-８，记录住院期间机械通气时间、住ＩＣＵ时间、治疗后７ｄ生存率、不 良反应。结果　治疗后与其他各组比较，Ｄ组支气管肺泡灌洗液中ＴＮＦ-α、ＩＬ-６、ＩＬ-８水平下降最明显，机械通气时间、住 ＩＣＵ时间最短，治疗后７天生存率最高，差异均有统计学意义（Ｐ均＜０．０５）；且Ｄ组未见不良反应明显增加。结论　血必 净联合乌司他丁静脉注射可显著降低ＡＲＤＳ患者支气管肺泡灌洗液中ＴＮＦ-α、ＩＬ-６、ＩＬ-８水平。 [ABSTRACT FROM AUTHOR]

[Objective] The study aimed to investigate the effect of macular pollutants on the release of pyrolytic products from cigarette paper, and provide a method basis for the classification of macular pollutants. [Method] The pyrolytic particulate matter of different cigarette papers was captured by a self-built temperature-controlled pyrolytic device. The differences in pyrolytic components of different cigarette paper samples were analyzed by using GC-MS. And the classification of macular pollutants was achieved using principal component and cluster analysis based on the differences in pyrolytic products. [Result] The different pollutants changed the microscopic surface morphology of cigarette paper fibers. And the pyrolytic products chromatograms of all the contaminated cigarette papers exhibited a similarity of less than 0.417 when compared with that of blank cigarette papers. A total of 78 kinds of pyrolytic products were detected in the blank cigarette papers, including ketones, phenols, benzene derivatives, aldehydes, furans, alcohols, heterocycles and other chemicals, etc. Moreover, the pyrolytic products released from contaminated cigarette paper not only had common components with blank cigarette paper, but also had many unique pyrolysis components, and there were significant differences in the number and content distribution of various pyrolysis components. Specifically, after the contamination of Premalube red and Nordic grease, the phenolic compounds in the pyrolytic products significantly reduced from 36.07% to 5.58% and 8.32%, respectively. Meanwhile the release of hydrocarbons remarkably increased, but the composition and distribution of hydrocarbon substances exhibited significant differences. Flavor and sugar contaminants reduced the content of aldehydes, ketones, phenols, benzene rings and heterocyclic pyrolytic components in the pyrolytic products, but their own unique propylene glycol/glycerol solvents or esters could release to the particulate matter during the heating process through the evaporation. Principal component analysis based on pyrolytic products showed that cigarette papers contaminated with different pollutants could be effectively distinguished from the blank sample. Hierarchical clustering analysis also showed that samples contaminated with grease could be classified into one, and cigarette papers contaminated with flavor and sugar could be grouped into another one. [Conclusion] The results in the study would be beneficial for the classification and identification of macular pollutants source based on the differences in pyrolytic products that were captured by self-built temperature-controlled pyrolytic device and analyzed by multiple statistical methods. [ABSTRACT FROM AUTHOR]

Objective: To investigate the predictive value of serum β2-microglobulin(β2-MG), soluble receptor for advanced glycation end-products(sRAGE) combined with prognostic nutrition index(PNI) for death in patients with primary treatment multiple myeloma(MM). Methods: 80 patients with MM initially diagnosed in Maanshan People's Hospital from January 2018 to January 2020 were selected. Serum β2-MG, sRAGE, albumin and peripheral blood lymphocyte count levels of all patients were detected before treatment, and PNI was calculated. The survival of patients with MM was recorded during the follow-up period of 24 months after discharge. The risk factors affecting the death of patients with MM at 24 months after discharge were analyzed and the clinical value of β2-MG, sRAGE and PNI combined in predicting the death of patients with MM at 24 months after discharge was analyzed. Results: During the follow-up period, 1 case was lost to follow-up, 24 cases died, and 55 cases survived. The serum β2-MG level in the death group was higher than that in the survival group (P＜0.05), and the serum sRAGE level and PNI were lower than those in the survival group (P＜0.05). R-ISS Ⅲ and increased β2-MG levels were risk factors for death in patients with MM within 24 months after discharge (P＜0.05), and increased sRAGE level and PNI were protective factors (P＜0.05). The area under curve of β2-MG, sRAGE and PNI predicting the death of patients with MM within 24 months after discharge were 0.776, 0.720 and 0.717, respectively. The area under curve of combining three indicators predicting the death of patients with MM within 24 months after discharge was 0.890, which was higher than that predicted by individual indicators. Conclusion: Increased serum β2-MG level and decreased sRAGE level and PNI in patients with MM are associated with death within 24 months after discharge. The combination of β2-MG, sRAGE and PNI could effectively assist in evaluating the prognosis of patients with MM. [ABSTRACT FROM AUTHOR]

Objective　 To investigate the effect of iso-suillin on the biological characteristics of human gastric cancer cells SGC-7901 and its mechanism. Methods　 SGC-7901 cells in the logarithmic growth phase were treated with 2. 83， 5. 66 and 11. 32 μmol/L iso-suillin， respectively， which were taken as the 2. 83 μmol/L iso-suillin group， 5. 66 μmol/L iso-suillin group and 11. 32 μmol/L iso-suillin group， respectively. Meanwhile， cells in the 0 μmol/L iso-suillin group were added with isovolemic normal saline only. The proliferation of SGC-7901 cells was detected by CCK-8， the apoptosis was detected by flow cytometry， the cell invasion was detected by Transwell， and the cell migration was detected by cell Scratch test. The colony-forming capacity was determined by clonal formation assay， and the mRNA expression levels of Ecadherin， N-cadherin and Vimentin were detected by real-time PCR. PI3K， p-PI3K， Akt and p-Akt were detected by Western blotting， and the reactive oxygen species （ROS） level was detected by flow cytometry. Results　 Compared with the 0 μmol/L iso-suillin group， the proliferation， invasion， migration and colony-forming capacity decreased in the 2. 83， 5. 66 and 11. 32 μmol/L iso-suillin groups（ all P<0. 05）； the proliferation， invasion， migration and colony-forming capacity decreased with the increased doses of iso-suillin（ all P<0. 05）. Compared with 0 μmol/L iso-suillin group， the apoptosis rates in the 2. 83， 5. 66 and 11. 32 μmol/L iso-suillin groups increased （all P<0. 05）， and they increased with the increased doses of iso-suillin. Compared with the 0 μmol/L iso-suillin group， E-cadherin mRNA levels significantly increased in the 2. 83， 5. 66 and 11. 32 μmol/L iso-suillin groups（ all P<0. 05）， but the mRNA levels of N-cadherin and Vimentin decreased （all P<0. 05）； the mRNA levels of E-cadherin increased， while the mRNA levels of N-cadherin and Vimentin decreased gradually with the increased doses of iso-suillin （all P<0. 05）. Compared with 0 μmol/L iso-suillin group， ROS fluorescence intensity， p-PI3K and p-Akt protein levels in the 2. 83， 5. 66 and 11. 32 μmol/L iso-suillin groups increased （all P<0. 05）； the fluorescence intensity of ROS， p-PI3K and p-Akt protein levels increased gradually with the increased dose of iso-suillin（ all P<0. 05）. Conclusion　 Iso-suillin can regulate the proliferation， apoptosis， invasion， migration and colony-forming capacity of SGC-7901 cells with a dose-dependent manner， and its mechanism may be that it indirectly inhibits the epithelial-mesenchymal transition process of SGC-7901 cells through ROS-PI3K/Akt signaling pathway. [ABSTRACT FROM AUTHOR]

Objective　We aimed to explore the anti-inflammatory molecular mechanism of Xijiao Dihuang Decoction combined with Yinqiao Powder (XDY) in macrophages infected with influenza virus. Methods　Genes related to human macrophages infected by influenza virus PR8 were screened by the gene chip published in the GEO database. The intersection of the active components of XDY and the related differential genes of human macrophages infected by influenza virus PR8 was input into the STRING database to obtain the interaction relationship between target proteins. The core target was obtained according to the degree of protein interaction. The protein-protein interaction network was visualized with Cytoscape 3. 7. 2 software, and topology analysis was performed. The Metascape database was used for Kyoto Encyclopedia of Genes and Genomes(KEGG) pathway enrichment analysis. Macrophages J774A. 1 were divided into the normal group, the model group(influenza virus PR8 infection), and the XDY group (PR8 + 162. 5 mg/ L XDY) and treated for 24 h. The mRNA levels of Toll-like receptor 4(TLR4), myeloid differentiation factor 88(MyD88), TNF receptor-associated factor 6(TRAF6), tumor necrosis factor-α(TNF-α), and interleukin-6(IL-6) were detected by real-time PCR; nuclear factor-κB(NF-κB) translocation was detected by immunofluorescence; and the levels of TNF-α and IL-6 in the supernatant were detected by ELISA. Results In total, 104 common targets were obtained by analyzing the intersection of the differentially expressed genes in the PR8 and XDY groups. Pathway enrichment analysis showed that the effects of XDY on influenza may be mediated by the TLR4/NF-κB pathway. PCR result showed that compared with the model group, the mRNA levels of TLR4, MyD88, TRAF6, TNF-α, and IL-6 were lower in the XDY group (P <0. 01). Immunofluorescence result showed that compared with the model group, the nuclear localization of NF-κB in the XDY group was decreased, and the nuclear-cytoplasmic ratio was also decreased (P<0. 01). ELISA result showed that compared with the model group, the levels of TNF-α and IL-6 in the supernatant in the XDY group were decreased (P<0. 01). Conclusion　Xijiao Dihuang Decoction combined with Yinqiao Powder can regulate the TLR4/NF-κB pathway to reduce the inflammatory response in macrophages infected with influenza virus. [ABSTRACT FROM AUTHOR]

Objective To observe the effects of iso-suillin on the proliferation and apoptosis of human hepatocellular carcinoma cells, and to explore the related mechanism based on the phosphatidylinositol 3-kinase (PI3K) /protein kinase B (Akt) pathway. Methods Human hepatocellular carcinoma cell line HepG2 in the logarithmic growth phase was divid‐ ed into the iso-suillin group, activator group, and control group. Cells in the iso-suillin group were added with 50 μg/mL iso-suillin, the activator group with 50 μg/mL iso-suillin and 20 μg/mL PI3K/Akt pathway activator Fumonisin B1, and the control group were only added with equal volume of normal saline without any drugs. MTT was used to measure the pro‐ liferation inhibition rate of each group at 24, 48 and 72 h after intervention. Hoechst 333258 fluorescence staining method was used to measure the apoptosis rate. Apoptosis-related proteins (Bax, Bcl-2, Caspase-3) and PI3K/Akt pathway relat‐ ed proteins (PI3K, pPI3K, pAkt) were detected by Western blotting. Results OD values of the iso-suillin group were lower than those of the activator group and control group at 24, 48 and 72 h after intervention, and inhibition rates of cell proliferation were higher than those of the activator group and control group (all P<0. 05) . The OD value of the iso-suillin group showed a decreasing trend, and the inhibition rate of cell proliferation showed an increasing trend over time (both P< 0. 05) . The apoptosis rate and protein expression levels of Bax and Caspase-3 in the iso-suillin group were higher than those in the activator group and the control group, while the protein expression levels of Bcl-2, PI3K, pPI3K, and pAkt were lower than those in the activator group and the control group (all P<0. 05) . Conclusion Iso-suillin can inhibit the proliferation and induce apoptosis of HepG2 cells, and the mechanism may be related to the inhibition of PI3K/Akt path‐ way. [ABSTRACT FROM AUTHOR]

Objective: To compare the effect of tail vein injection of bone marrow mesenchymal stem cell on type 1 diabetes(T1D) in immature C56bl/c mice. Methods: 3 weeks old C56bl/c mice was injected streptozotocin(STZ) 50 mg/kg by intraperitoneal for 5 days. BMSCs were isolated from Tibia and Femur of C56bl/c mice by enzyme digestion and bone slice method. The induced differetiation ability of the BMSCs was detected by alkaline phosphatase staining and Oil-red-O staining. The flow cytometry was used to detect the phenotypes of BMSCs. The T1D mice were treated with normal saline and different doses of BMSCs by intravenous infusion, and the body weight and blood glucose of T1D mice were detected regularly. The pancreas of the T1D mice was taken for pathological analysis which were treated by BMSCs for 28 days later. Results: (1)The 3-weeks-old C56bl/c mice showed slow weight gain and significantly blood glucose 14 days after the STZ injection. (2) The BMSCs were exhibited long spindle like fibers. ALP and Oil-red-O staining showed that BMSCs could be induced to Osteoblast and adipocyte. Flow cytometry analysis showed that BMSCs overexpressed CD29, CD90 and CD105, and rarely expressed CD31, CD34 and CD45. (3) The blood glucose of T1D mice decreased and remained stable which treated with BMSCs. Pathological examinations on pancreas islets by H.E. staining and insulin immunohistochemistry demonstrated that the pancreas islet of T1D mice in MSC treatment groups were recovered with the time of treatment, while the without any treatment group of the pancreas islet was getting worse. Conclusion: BMSCs had a significant effect on T1D immature mice. [ABSTRACT FROM AUTHOR]

Objective: Study on the role of Nocardia rubra cell wall skeleton (N-CWS) in adjuvant treatment of tumors. Methods: Among patients with solid tumors treated by the Department of Oncology, Peking Union Medical College Hospital in the past 5 years, patients who were willing to receive N-CWS adjuvant therapy were subcutaneously injected with Nocardia red cell wall skeleton (brand name Aikejia), 200～400 µg / time Twice a week, monitor the changes in tumor markers serum carcinoembryonic antigen (CEA), imaging and physical condition scores to evaluate the efficacy of N-CWS. Results: After the addition of N-CWS to Case 1, CEA decreased and remained low for about 4 months, and the ECOG score remained at 0 to 1. In case 2, CEA decreased and remained stable for two months after N-CWS was added, and ECOG was 0 to 1 point. The patient's physical strength improved after using the consciousness. The CEA of case 3 remained minimum after 6 cycles of chemotherapy and remained stable for 1 month. ECOG stays at 0 ~ 2 points. The CEA of cases 1 and 2 remained stable for higher than case 3, and the ECOG score remained lower than case 3. Conclusions: With the use of N-CWS as an adjuvant therapy for tumors, the CEA level of patients decreased, and the patient's physical condition improved, which significantly improved the patient's symptoms. [ABSTRACT FROM AUTHOR]

In order to study the chemical constituents, the ethanol extract of Ainsliaea yunnanensis was isolated and purified by the normal pressure silica column chromatography, Sephadex LH-20, the low pressure cyano chromatography and preparative high pressure liquid chromatography. Ten compounds were isolated and identified as 3β-hydroxystigmast-5-en-7-one(1), 3β-hydroxystigmast-5,22-dien-7-one(2), β-sitosterol(3), stigmasterol(4), β-daucosterol(5), stigmasterol glucoside(6), 3,5-O-dicaffeoylquinic acid(7), 3,5-O-dicaffeoylquinic acid methyl ester(8), caffeic acid(9), protocatechuic acid(10). All compounds are isolated from this plant for the first time, and compounds 2 and 8 are isolated from this genus for the first time. The discovery of the compounds may enrich the chemical constituents of A. yunnanensis and provide a theoretical basis for its rational and effective application. [ABSTRACT FROM AUTHOR]

To identify methods for detection of para-Bombay blood group in order to guide clinical blood transfusion. Methods For the blood donors whose ABO blood growp results were not matched in the forward and reverse typing tests, immuno-serological methods were performed to identify the ABO blood group, H antigen, Lea antigen, Leb antigen, and irregular antibody. Besides, the blood genomic DNA was extracted and used to identify the genotypes of ABO blood group, and FUTl genes were sequenced. Results ABO blood group's positive stereotype response in blood donors was type O, and the negative stereotype response was type A, which were not consistent. The immuno-serological detection results were negative in H antigen, positive in Lea antigen, and positive in Leb antigen, so they were classå±±ed as Le (ab + ) type according to the erythrocyte Lewis system; irregular antibodies were anti-H antibodies. The donor's ABO blood group genotype was AA. FUTI gene sequencing revealed a homozygous mutation of C > T in exon 658 of FUTI gene, which was expected to lead to the conversion of arginine to cysteine during translation. Under these conditions, it was judged as a para-Bombay blood group type. Conclusions One case of clinically rare type A para-Bombay blood group is found. For the blood samples which are difficult to identify clinically and are suspected to be rare Bombay-like blood groups, genetic detection can be carried out to determine their blood types and guide clinical blood transfusion when immuno-serological identification is not clear. [ABSTRACT FROM AUTHOR]

Objective: To evaluate the effect of two-photon in vivo imaging on detecting the blood brain barrier (BBB) injury in the ultra-early stage of cerebral ischemic stroke. Methods: Twelve clean grade C57BL/6 healthy male mice aged 8-12 weeks were randomly divided into Sham group and middle cerebral artery occlusion (MCAO) group, which were sham operated or middle cerebral artery occluded, respectively. After 60 min of ischemia, MCAO mice were treated with reperfusion for 30-60 min after the suture being removed. The vessels and the neutrophils of mice in the two groups were labeled intravenously with Alexa-Fluora-488 conjugated dextran and rhodamine 6G, respectively. The integrity of BBB was detectedintravenous injection of tetramethylrhodamine-5-maleimide (TMR). Before and after the stroke, the real-time changes of the fluorescence intensity of the inside and outside cerebral vessels of mice in the MCAO group were observedtwo-photon fluorescence microscopy. Results: The fluorescence intensity of TMR in the external cerebrovascular of mice in the MACO group was significantly increased within 30-60 min after stroke (P0.000), suggesting there existed tracer leakage. Compared with the Sham group, the movement of neutrophils in the blood vessels of mice in the MACO group was significantly slowed down (P0.000). Conclusion: Two-photon in vivo imaging can be used to detect the BBB injury in the ultra-early stage of cerebral ischemic stroke, which provides a certain reference value for clinical application. [ABSTRACT FROM AUTHOR]