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Objective To investigate the expression of pyruvate kinase M2 ( PKM2) in intrahepatic cholangiocarcinoma (ICC) and the effect of PKM2 on the proliferation and epithelial-to -mesenchymal transition (EMT) of ICC cells. Methods PKM2 expression was evaluated in ICC tissues and cell lines by Real-time quantitative reverse-transcription polymerase chain reaction ( RT-PCR), and immunohistochemistry assays. In vilro, we knocked down PKM2 expression in ICC cell lines and investigated the biological function and the underlying mechanism of PKM2 in ICC. Results RT-PCR results showed that PKM2 was highly expressed in ICC ( P·<0. 05). Moreover. PKM2 knockdown inhibited cell proliferation and the invasive capacities of ICC cells. and inhibited Wnt/β-Catenin signaling. which subsequently regulated EMT signaling pathway in vitro. Conclusion PKM2 expression in cholangiocarcinoma is significantly higher than that in adjacent tissues. PKM2 can regulate EMT and invasion and metastasis of ICC via the Wnt/β-Catenin signal. [ABSTRACT FROM AUTHOR]

In this paper, methyl eleostearate was prepared via the transesterification reaction betweent ung oil and methanol, and then was used to synthesize methyl eleostearate-maleic anh ydride CME-MA) adduct by D-A reaction under UV light. The structure of ME-MA was also identified and characterized by FTIR spectra, UVvis spectra and 1H NMR. Finally, ME-MA adduct was applied to the polyurethane coatings to study the effect of photoinitiator, reactive diluent and ME-MA adduct on the cured films. The experimental results concluded that the optimum formula conditions were as follows: the photoinitiator was TPO, the reactive diluent was HDDA, the amount of TPO was 5% and the addition amount of ME-MA was 20%. The cured film formation possessed good performance, including the highest gel fraction of 96.5%, tensile strength of 11.3 MPa and elongation at break of 23.2%. [ABSTRACT FROM AUTHOR]

Tung oil, as an important strategic biomaterial and renewable biomass resource, has attracted increasing attention due to its high value of research and application. In this study, eleostearic acid glyceride (the main component of tung oil) was transformed to methyl eleostearate (ME) by transesterification, and then reacted with catechol (CAT) via Friedel-Crafts alkylation using SO3 H-functionalized ionic liquid as catalyst. The alkylation products (CAT-ME) similar to urushiol (urushiol analogues) were successfully synthesized, and confirmed by the characterization results of infrared spectroscopy, UV visible spectroscopy, NMR spectra and IIPLC-MS spectra. [ABSTRACT FROM AUTHOR]