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为提升金鲳鱼蛋白乳液的稳定性，改善金鲳鱼蛋白的功能特性，以金鲳鱼蛋白-茶皂苷复合物为乳化剂、大豆油为油相，采用高强度超声（HIUS）辅助乳化制备金鲳鱼蛋白-茶皂苷复合乳液，测定超声处理前后不同乳化剂与油相比例的金鲳鱼蛋白-茶皂苷复合乳液的乳化性、粒径分布、Zeta-电位、流变性、微观形态及贮藏稳定性。结果表明：与未超声处理金鲳鱼蛋白-茶皂苷复合乳液相比，超声处理后乳液的乳化活性指数和乳化稳定性指数总体增高，粒径减小，Zeta-电位的绝对值增加，流动指数升高，黏度系数降低，乳液液滴分布更均匀，乳液液滴更小，贮藏稳定性较好。综上，HIUS处理有助于改善金鲳鱼蛋白-茶皂苷的乳化性能。In order to improve the stability of the golden pompano protein emulsion and improve the functional properties of golden pompano protein, golden pompano protein-tea saponins composite emulsion was prepared by high intensity ultrasound (HIUS)-assisted emulsification with golden pampano protein-tea saponins complex as emulsifier, soybean oil as oil phase, the emulsifying properties, particle size distribution, Zeta-potential, rheological properties, microscopic morphology and storage stability of golden pompano protein-tea saponins composite emulsion with different emulsifier-oil ratio before and after ultrasonic treatment were measured. The results showed that compared with emulsion without ultrasonic treatment, the emulsifying activity index and emulsifying stability index of the golden pompano protein-tea saponins composite emulsion after HIUS treatment increased in general, the particle size decreased, the absolute value of Zeta-potential increased, flow index increased, and viscosity coefficient decreased. The distribution of emulsion drops was more uniform, the emulsion drops were smaller, and the storage stability was better. In summary, HIUS treatment is helpful to improve the emulsifying properties of golden pompano protein-tea saponins.

提出了OCDMA系统中所使用的多维码的概念并对其性能进行了分析。重点介绍了光素数码、二维光正交码、二维素数码及三维码。

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Engineering for Thermal Energy & Power / Reneng Dongli Gongcheng is the property of Journal of Engineering for Thermal Energy & Power and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Feed Research is the property of Editorial Board of Feed Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To explore mechanism of long non-coding RNA maternally expressed gene 8 (LncRNA MEG8) mediates immune escape in colorectal cancer (CRC) cells by regulating MICA/B via targeting miR-15a-5p. Methods: RT-qPCR and Western blot were used to detect expressions of MEG8, miR-15a-5p, MICA, MICB, NKG2D protein in CRC tissue and cell lines; dual luciferase experiment was used to verify regulatory relationship of MEG8 on miR-15a-5p; NC, MEG8, miR-NC and miR-15a-5p were respectively or co-transfected into SW480 and SW620 cells by liposome transfection, and recorded as NC group, MEG8 group, MEG8+miR-NC group, MEG8+miR-15a-5p group; NK cells were cocultured with SW480 and SW620 cells respectively; ELISA was used to detect TNF-α and IFN-γ levels in coculture medium; CCK-8, EdU staining, Transwell assay were used to detect cell proliferation, migration and invasion ability; RT-qPCR and Western blot were used to detect MEG8, miR-15a-5p, MICA, MICB, NKG2D protein levels in cells. Results: Compared with adjacent cancer tissues or normal colon epithelial cells, MEG8, MICA, MICB, NKG2D mRNA and protein levels in CRC tissues and cell lines were decreased, while miR-15a-5p level was increased (P<0.05). MEG8 targeting regulated miR-15a-5p. In cocultivation system, compared with NC group, MICA, MICB, NKG2D protein levels, TNF-α, IFN-γ levels in cocultured supernatant in MEG8 group were significantly increased, after 1 d, 2 d, and 3 d of cell culture, OD value, EdU positive rate, number of migrating and invading cells were significantly decreased (P<0.05); overexpression of miR-15a-5p could partially reverse effects of overexpression of MEG8 on MICA, MICB, NKG2D, TNF-α, IFN-γ levels in cells, proliferation, invasion and metastasis ability( P<0.05). Conclusion: MEG8 promotes NK cell activity and inhibits CRC cell immune escape by regulating MICA and MICB expressions via targeting miR-15a-5p. [ABSTRACT FROM AUTHOR]

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[Objective]This work aims to explore the possible regulatory mechanism of endogenous hormones on the differential accumulation of carotenoids in different organs of Sarcandra glabra.[Method]In this study, metabolomics was used to analyze the differential distribution of endogenous hormones and carotenoid metabolites in different organs of S. glabra. Combined with transcriptomics technology, the differential enzyme genes related to carotenoids in S. glabra were excavated, and the transcription factors involved in the regulation of differential enzyme genes and their hormone-related cis-response elements were further predicted, so as to analyze the possible regulation of endogenous hormones on the differential accumulation of carotenoids.[Result]The contents of seven endogenous hormones such as indole3-carboxylic acid,[(-)-jasmonic acid], ethyl dihydrojasmonate, castasterone and brassinolide were high in the leaves. Consistent with the enrichment sites of 8 differential metabolites, such as canthaxanthin, echinenone, neoxanthin and nostoxanthin; and abscisic acid, gibberellin 4,5-methoxysalicylic acid, dihydrojasmonic acid and 5,6-dihydroxyindole was high in the roots, it was consistent with the accumulation site of 3 differential metabolites, abscisic aldehyde, 4,4'-aiapolycopenedial and antheraxanthin. The key transcription factor MYB106, SPL1, NAC015, ERF064, WRKY44 and BHLH116 responded to the stimulation of plant hormones such as AUX, SA, GA, ABA, and Me_ JA, and participate in the regulation of carotenoid synthesis pathway DXS, VDE, ABA2, AOG, ZEP, CYP97C1, DWARF27, CRTISO, LCYB expression of these 9 metabolic enzymes. Quantitative real-time polymerase chain reaction(RT-qPCR)results showed that the expression trends of 8 randomly selected differentially expressed genes were consistent with the transcriptome sequencing results.[Conclusion]The 15 differential endogenous hormones and 11 differential metabolites related to carotenoids in the leaves and roots of Sarcandra glabra were screened, and 6 transcription factors were predicted to be involved in the regulation of 16 metabolic enzymes. [ABSTRACT FROM AUTHOR]

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Copyright of Chinese Journal of Contemporary Pediatrics is the property of Xiangya Medical Periodical Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Storage & Process is the property of Tianjin Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Denitrification is one of the most important reactive nitrogen removal pathways in the soil nitrogen cycle. In this process, nitrate is reduced to gaseous nitrogen nitrous oxide（N2O）or nitrogen（N2）, which leaves the soil and enters the atmosphere. The stoichiometric ratio of its gaseous products N2O/N2 is a hot topic in soil denitrification research. Reasonable regulation of this ratio is an important way to reduce greenhouse gas emissions from agricultural sources. In the soil environment, the ratio of denitrification products is affected by many factors. In our view, nitrate content, oxygen, and copper availability are the key factors mediating the change of the product ratio, while electron donors, pH, and moisture are general factors. These factors can directly affect the denitrification process by affecting denitrifying enzyme activity, denitrifying microorganism abundance, and community structure. They can also indirectly affect the ratio by changing the soil microenvironment of denitrification. This paper reviews the progress made toward controlling the factors affecting the soil denitrification gaseous product ratio N2O/N2 in recent years and discusses the response mechanism of the denitrification product ratio to different influencing factors to provide some inspiration for future related research. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Nanjing Agricultural University / Nanjuing Nongye Daxue Xuebao is the property of Journal of Nanjing Agricultural University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This paper reviews the synthesis methods and basic properties of high temperature resistant natural modified AMPS filtrate reducer, high temperature resistant zwitterionic AMPS filtrate reducer and high temperature resistant rigidized modified AMPS filtrate reducer. And introduces the introduction of silicon, starch and graphene into natural modified AMPS filtrate reducer to improve salt and calcium resistance, the addition of cationic groups to ionic AMPS filtrate reducer to enhance electrostatic adsorption capacity and the introduction of rigid groups to rigidized modified AMPS filtrate reducer to increase molecular rigidity ・ This paper also evaluates the research directions of natural modified AMPS fluid loss additive, amphoteric AMPS fluid loss additive and rigidified AMPS fluid loss additive, with a view to providing a good reference for the development of new natural modified materials, the development of synthetic materials with multiple properties, and the introduction of other rigid groups with a ring structure. [ABSTRACT FROM AUTHOR]

Copyright of University Chemistry is the property of Peking University, College of Chemistry & Molecular Engineering and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In response to the current situation that most of the bait feeders in pond culture rely on manpower, which is labor-intensive, and it is difficult to feed the baiting boats on steep sloping banks, an energy-saving feeding truck without power discharging has been designed. The truck uses a rotating discharging valve for feeding, and a scissor lift platform for adjusting the height of feeding. Through the design of the spherical limit rotating connector located at the discharge port of the rotating discharging valve to adjust the inclination angle of the feeding tube to realize the non-powered discharging, a new type of telescopic feeding tube is designed based on cylindrical cam. The feeding truck can realize directional, fixed-distance and fixed-point feed addition through the movement of the truck body, the lifting and lowering of the scissor lift platform, and the adjustment of the inclination angle and length of the feeding tube. The prototype of the feeding truck was made, and the feeding test was carried out with the baiting boat and stationary baiting machine as the feeding target, and the test results show that the feeding truck has the universality for different specifications of feeds, and the telescopic length of 1.2 m and the lifting height of 1.5 m can satisfy the feeding needs of the baiting boat and stationary baiting machine, and the adjusting is flexible, and the rate of feeding is 6.2 kg/min. The designed feeding truck is flexible in use, and it can replace the manual feeding to the baiting boat and stationary baiting machine. It can replace manual feeding for baiting boat and fixed baiting machine, reduce manual labor intensity, improve the mechanization level of breeding process, and lay the foundation for further automation and intelligence. [ABSTRACT FROM AUTHOR]