To investigate the effect of the red/blue light quality ratio on the growth and metabolites accumulations of A.roxburghii and A.formosanus, the tissue culture plants of A. roxburghii and A. formosanus were treated with three LED lights of R2 B1(with Red/Blue ratio of 2:1),R1 B2(with Red/Blue ratio of 1:2),and W(white light). After 60 d of illumination, the content of chlorophyll, soluble sugar, soluble protein, total phenols, and total flavonoids were determined by spectrophotometry, and the content of quercetin, isorhamnetin, kaempferol, ferulic acid, and chlorogenic acid were determined by HPLC combined with standards.Under the R2 B1 light, the fresh weight, dry weight, contents of chlorophyll and phenolic acid compounds of the two species reached the highest.The soluble protein content of the two species under the R1 B2 light reached the lowest.Under the R2 B1 light, the total phenol content of A.roxburghii was significantly higher than that of W.But under the R1 B2 light, the content of soluble sugar and total flavonoids of A.formosanus was significantly higher than that of W. Under the R2 B1 light, the contents of quercetin and the isorhamnetin in A.roxburghii were(36.52±0.91)and(61.35±2.45) μg.g-1,respectively, and the content of chlorogenic acid in A. formosanus was(40.09±3.79) μg.g-1.Therefore, the R2 B1 compound light can be used as a suitable light formula for A.roxburghii and A. formosanus,and it can provide scientific basis for the application of LED in the tissue culture of A. roxburghii and A. formosanus. [ABSTRACT FROM AUTHOR]