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BACKGROUND: Cryopreservation can better ensure the integrity of the vascular structure. How to reduce its immunogenicity to improve rejection after transplantation has attracted more and more attention. OBJECTIVE: To review the research progress of freezing treatment to reduce vascular immunogenicity after allogeneic vascular transplantation in order to provide a reference for clinical research. METHODS: A systematic search of Chinese and English databases CNKI, WanFang and PubMed, as well as online websites Baidu and Google Scholar since the establishment of the database has published literature on reducing vascular immunogenicity after allogeneic vascular transplantation. Keywords were “cardiovascular disease, endothelial cells, cryopreservation, blood vessel transplantation or vascular graft, immunogenicity, immune rejection, allograft or allogeneic transplantation or allograft transplantation and cryoprotectant”. A total of 68 articles were included according to the inclusion and exclusion criteria. RESULTS AND CONCLUSION: (1) The review found that the rejection of allogeneic vascular transplantation can be reduced by improving the existing freezing technology, which mainly involves the selection of freezing and thawing methods and cryoprotectants. (2) The existing research suggests that the freeze-drying method is superior to the low-temperature cryopreservation, but due to the limited conditions, it is still dominated by low-temperature cryopreservation. Among them, vitrification cryopreservation, slow rewarming and the use of stainless steel and even silver-containing materials are better than programmed cryopreservation and rapid rewarming. (3) The combined selection of permeable and non-permeable cryoprotectants can further reduce the occurrence of rejection while reducing their toxicity. [ABSTRACT FROM AUTHOR]

Objective To study the intervention effect of methylprednisolone (MP) on high pressure injection injuries (HPII) of lower limbs of rats. Methods Thirty rats were randomly divided into control group, model group (high-pressure injection injury model) and treatment group (tail vein injection of MP 30 mg/kg). After 6 hours, the jet area and limb circulation were observed, blood vessels, nerve and muscle tissues were examined by HE staining microscopy. Liver index was calculated and serum TNF-α, IL-1β and IL-6 were tested by ELISA. Comparison between multiple groups was performed by One-way Anova. Results Compared with the control group, limb circulation, blood vessels, nerves and muscles were significantly damaged.The liver index decreased while the serum content of TNF-α, IL-1β, and IL-6 all increased significantly (P＜0.05). Compared with the model group, the above indicators improved significantly in the treatment group (P＜0.05). Conclusions Injection of MP after high-pressure injection injuries can alleviate limb damage, reduce liver load and improve serum inflammatory markers of injured rats. [ABSTRACT FROM AUTHOR]

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BACKGROUND: Small-diameter blood vessel prosthesis has a wide application value in the treatment of peripheral vascular diseases, but the problem of graft failure due to early thrombosis needs to be solved. OBJECTIVE: To summarize research progress on early thrombosis and prevention of small-diameter blood vessel prosthesis. METHODS: The relevant literature published on CNKI, Wanfang, PubMed, and Google Scholar databases from 2000 to 2020 was retrieved by the first author. The Chinese search terms were “small-diameter blood vessel prosthesis, small-diameter tubular stents, tissue-engineered blood vessels, antithrombotic, thrombosis, hemocompatibility, hydrophilicity, endothelialization”. The English search terms were “small-caliber vascular graft, vascular tissue engineering applications, endothelialization, hemocompatibility, surface modification”. The relevant literature on the pathophysiological mechanism and prevention of small-diameter blood vessel prosthesis thrombosis in China and abroad was retrieved, and the surface modification and endothelialization of biological materials were taken as the inclusion criteria. Finally, 57 articles were selected for review. RESULTS AND CONCLUSION: Prevention of thrombosis is a hotspot and a difficulty in the study of small-diameter blood vessel prosthesis. The research progress at home and abroad in recent years shows that the early thrombosis of small-diameter blood vessel prosthesis is related to plasma protein adsorption and contact activation of coagulation factors. By improving the surface hydrophilicity, releasing antithrombotic substances, planting seed cells and target gene induction, the thrombosis of small-diameter blood vessel prosthesis has been improved to a certain extent, but it is still in the experimental stage, and there is still a certain distance from clinical application. Therefore, it is necessary to further explore or optimize a more suitable vascular material to provide more choices for clinical application. [ABSTRACT FROM AUTHOR]