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Objective: To observe the effect of early electroacupuncture intervention on the expression of TARDNA binding protein 43 (TDP- 43) and HMGB1/RhoA signaling pathway in the cerebral cortex of amyotrophic lateral sclerosis (ALS) mice, and to explore the potential mechanism of early electroacupuncture intervention in improving motor function in ALS mice. Method: SOD1G93A gene phenotype mice were randomly divided into model group (SOD1G93A ), acupuncture intervention group (EA), riluzole group (Riluzole), and SOD1G93A negative mice in the same litter were blank control group (Control), with 15 mice in each group. The electroacupuncture group was given acupuncture of Baihui point, bilateral Tianzhu point, bilateral Tianshu point, 5 times/7 days, 7 days for a course of treatment, a total of 4 courses of treatment. The riluzole group was treated with riluzole 30 mg（/ kg·d) by gavage, once a day, five times a week for two weeks. The motor function of mice in each group was evaluated by hind limb functional neurological score and rotarod fatigue test, and the rate of TDP-43 positive cells in cerebral cortex was observed by immunofluorescence. The relative expression of Iba-1, HMGB1 or RhoA protein in cerebral cortex was detected by Western Blot. The levels of serum TNF-α and MCP-1 were detected by Elisa. The morphological changes of cerebral cortical neurons were observed by transmission electron microscopy. Result: Compared with the control group, the rotarod latency time was decreased and the neurological score was increased in the model group (P<0.01) . The contents of serum MCP-1 and TNF-α, the expression of Iba1, HMGB1 and RhoA protein in cerebral cortex and the rate of TDP-43 positive cells were increased in the model group (P<0.01) . Compared with the model group, the rotarod latency time of the electroacupuncture group and the riluzole group increased and the neurological score decreased (P<0.01, P<0.05), the serum MCP-1, TNF-α content and the cerebral cortex Iba-1, HMGB1, RhoA protein expression and TDP-43 positive cell rate decreased (P<0.01, P<0.05) . The results of electron microscopy showed that the structure of cortical neurons in the control group was normal, and the nerve cells in the model group exhibited obvious pathological changes. The damage of nerve cells in the electroacupuncture group and the riluzole group was reduced, the structure was relatively complete, and some normal organelles were detected. Conclusion: Electroacupuncture intervention can improve the motor function of ALS model mice. The mechanism may be related to the inhibition of HMGB1/RhoA signaling pathway and the reduction of microglia-induced neuroinflammation, and it is speculated that it has a positive effect on the reduction of ALS pathological substrate TDP-43 deposition. [ABSTRACT FROM AUTHOR]

目的 基于妥德淼健康行为模型, 研究老年患者门诊预约挂号的影响因素, 为优化门诊预约挂号服务、推 进老年患者预约挂号提供参考。方法以安德森健康行为模型为理论框架设计问卷, 采用方便抽样法向上海某 三级甲等综合医院门诊老年患者发敖调查问卷。将各维度因素纳入二分类logistic回归模型, 采用单因素分析 和logistic回归分析探讨老年患者门诊预约挂号的影响因素。结果共回收282份有效问卷, 预约挂号187人, 占 66.31%。曾使用过1~2种预约挂号服务、存在社会支持、距离医院路程80 min以上、收入为＞ 5 000-7 500元、就 诊科室预约率25.00%以上、有预约挂号使用意愿的老年患者更倾向于预约挂号。患慢性病数量在5种及以上的老 年患者更不倾向于预约挂号。结论老年患者门诊预约需求较大且受多种因素影响。须关注老年人预约挂号"痛 点", 关怀老年群体, 共建支持网络, 加强有效宣传, 优化医疗资源配置, 激发使用意愿. [ABSTRACT FROM AUTHOR]

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