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BACKGROUND: Shujin Jiannao Prescription is an empirical formula for the treatment of cerebral palsy in Dongzhimen Hospital, Beijing University of Chinese Medicine, with clear clinical efficacy, but the specific mechanism needs to be elucidated. OBJECTIVE: To explore the possible mechanism of Shujin Jiannao Prescription in treating cerebral palsy. METHODS: Sixty-four 7-day-old Sprague-Dawley rats were randomly divided into a normal group (n=12) and a model group (n=52). An animal model was established by the Rice-Vannucci method. After successful modeling, 52 model rats were randomly divided into control model group (n=12), minocycline group, and the low-, medium-, and high-dose groups of the Shujin Jiannao Prescription (n=10 per group). Rats in the minocycline group were given 40 mg/kg•d minocycline by gavage; rats in the low-, medium, and high-dose groups were given 4, 8, and 16 g/kg•d Shujin Jiannao Prescription granules by gavage, respectively; and rats in the normal group and control model group were given an equal dose of normal saline by gavage. Medication in each group was given once a day for 1 week. The rats in each group were evaluated behaviorally using suspension test, abnormal involuntary movement score, and Bederson score. The pathological changes of the cerebral cortex were observed by hematoxylin-eosin staining. The levels of tumor necrosis factor α, interleukin 1β, and interleukin 10 in the cerebral cortex were determined using ELISA. The positive expressions of Janus kinase 2 (JAK2), phosphorylated Janus kinase 2 (p-JAK2), phosphorylated signal transducer and activator of transcription 3 (p-STAT3) in the cerebral cortex were detected using immunohistochemistry. The protein expression levels of JAK2, p-JAK2, and p-STAT3 were detected using western blot. RESULTS AND CONCLUSION: Compared with the normal group, the suspension test score and involuntary movement score were decreased in the control model group (P < 0.01 or P < 0.05). The pathological results showed structural disruption of nerve cells, formation of large numbers of vacuoles, cell swelling, and increased intercellular space in the control model group. In addition, the expressions of tumor necrosis factor α and interleukin 1β in the cerebral cortex were significantly increased (P < 0.01), the expression of interleukin 10 was decreased (P < 0.05), and the protein expressions of JAK2, p-JAK2, and p-STAT3 in the cerebral cortex were significantly increased (P < 0.01) in the control model group compared with the normal group. Compared with the model group, minocycline and Shujin Jiannao Prescription at each dose could improve the behavioral indexes of rats (P < 0.01 or P < 0.05) and ischemic-hypoxic pathological changes were attenuated, with only a small amount of necrotic nerve cells and a few vacuoles, and reduced intercellular space. Moreover, the expressions of tumor necrosis factor α and interleukin 1β in the cerebral cortex were decreased in each drug group compared with the control model group (P < 0.05), while the protein expressions of JAK2, p-JAK2, and p-STAT3 in the cerebral cortex were significantly decreased (P < 0.01). The most obvious improvement was observed in the high-dose Shujin Jiannao Prescription group. To conclude, Shujin Jiannao Prescription can inhibit inflammation in the cerebral cortex of rats with hypoxic-ischemic brain injury. The mechanism may be related to the regulation of the JAK2/STAT3 signaling pathway. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Engineering Mechanics / Gongcheng Lixue is the property of Engineering Mechanics Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Beijing University of Technology is the property of Journal of Beijing University of Technology, Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Most scholars now believe that children with cerebral palsy who have severe spinal deformities in early childhood (< 15 years of age) may have a higher risk of progression of spinal deformities, which may result from imbalances in movement due to pelvic tilt, pain, etc. OBJECTIVE: To investigate the relationship between lumbar spine development and hip joint development in children with spastic cerebral palsy. METHODS: A retrospective analysis was performed in 102 children with spastic cerebral palsy admitted at Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine from January 2014 to December 2021. All admitted children had X-rays of the pelvic position and the lumbar lateral position. Anteroposterior X-ray of the pelvis was performed to measure femoral head migration percentage, central edge angle, neck-shaft angle, and acetabular index. The sagittal Cobb angle, sacral slope, arch-top distance, and lumbar lordosis index were measured by the lateral X-ray of the lumbar spine. Correlation of the two sets of indicators was further analyzed. All children were divided into normal group, risk group, hip subluxation group and total hip dislocation group according to their femoral head migration percentage, and the differences in lumbar spine indexes between groups were evaluated. RESULTS AND CONCLUSION: Pearson correlation analysis showed that the femoral head migration percentage was moderately positively correlated with sagittal Cobb angle and arch-top distance, and weakly positively correlated with lumbar lordosis index; the central edge angle was moderately negatively correlated with the arch-top distance and weakly negatively correlated with the sagittal Cobb angle; the neck-shaft angle was weakly positively correlated or not correlated with the sagittal Cobb angle and lumbar lordosis index; and the acetabular index was weakly positively correlated with the sagittal Cobb angle and arch-top distance. No statistically significant correlation was found between the remaining indicators. According to the femoral head migration percentage, the children were divided into four groups, including 25 cases in the normal group, 41 cases in the risk group, 27 cases in the hip subluxation group, and 9 cases in the total hip dislocation group. The sagittal Cobb angle was significantly increased in the risk group, the hip subluxation group and the total hip dislocation group compared with the normal group, showing an increasing trend group by group, and there were significant differences between groups (P < 0.05). Compared with the normal group, the lumbar lordosis index in the risk group and the hip subluxation group increased significantly, and there were significant differences between groups (P < 0.05). There was an increase trend in the lumbar lordosis index of the total hip dislocation group compared with the normal group. Compared with the normal group, the arch-top distance in the hip subluxation group and the total hip dislocation group increased significantly (P < 0.05), and there was a stepwise increasing trend. There was no significant difference in sacral slope between groups. To conclude, the development of the lumbar spine in children with cerebral palsy is closely related to the development of the pelvic hip joint, and the most obvious relationship is between lumbar lordosis and hip dislocation. [ABSTRACT FROM AUTHOR]

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Polymer Materials Science & Engineering is the property of Sichuan University, Polymer Research Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

BACKGROUND: Hemorheological changes following spinal cord injury can cause microcirculation disorder, induce tissue ischemia and hypoxia, and aggravate the secondary injury to spinal cord tissue. OBJECTIVE: To observe the changes in hemorheological indexes at different time points after tetramethylpyrazine intervention in rats with complete spinal cord transection. METHODS: Fifty-four 8-week-old female Sprague-Dawley rats were randomly divided into three groups (n=18 per group): sham operation group, model group, and tetramethylpyrazine group. Only laminectomy was performed in the sham operation group, while the T10 segment was completely transected with a 2 mm defect gap in the model and tetramethylpyrazine groups by a self-made double-edge microshear. After model preparation, tetramethylpyrazine group was given intraperitoneal injection of tetramethylpyrazine hydrochloride, 200 mg/kg per day, for 5 consecutive days. Abdominal aorta blood samples were taken 7, 14, and 28 days after operation for measuring whole blood viscosity (low, high and medium shear rates), plasma viscosity, hematocrit, erythrocyte aggregation index, erythrocyte deformability index, erythrocyte rigidity index, and erythrocyte electrophoresis index. RESULTS AND CONCLUSION: The whole blood viscosity (low, high, and medium shear rates) and plasma viscosity in the model group were significantly higher than those in the sham group at 7, 14, and 28 days after spinal cord injury (P < 0.05). Hematocrit was significantly higher but erythrocyte rigidity index and erythrocyte electrophoresis index were significantly lower in the model group than the sham group at 14 days after spinal cord injury (P < 0.05). Tetramethylpyrazine significantly reduced whole blood viscosity (low, high, and medium shear rates) and plasma viscosity at 7 days after spinal cord injury (P < 0.05). Tetramethylpyrazine significantly increased erythrocyte deformability index (P < 0.05) and certainly but not significantly improved blood viscosity at 14 days after spinal cord injury (P > 0.05). Tetramethylpyrazine significantly improved whole blood viscosity (hyposhear) and plasma viscosity at 28 days after spinal cord injury (P < 0.05). To conclude, after spinal cord injury, the whole blood viscosity and plasma viscosity can increase and erythrocyte indexes can also change to varying degrees. The use of tetramethylpyrazine can improve the deterioration of early hemorheological indexes and improve microcirculation disorders. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Chinese Institute of Food Science & Technology is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Electronic Components & Materials is the property of Electronic Components & Materials and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu is the property of Chinese Journal of Tissue Engineering Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

OBJECTIVE: Cerebral palsy seriously affects children's physical and mental health due to its high morbidity and disability rate. Ankle-foot orthoses are an important treatment for this disease. However, it is not very clear about the clinical effect of ankle-foot orthoses on the motor function of children with cerebral palsy. Therefore, this paper systematically assesses the clinical effect of ankle-foot orthoses on the improvement of motor function in children with cerebral palsy. METHODS: Literature retrieval was carried out in the electronic databases of PubMed, EMbase, Web of Science, Cochrane Library, CBM, CNKI, WanFang, and VIP. The time limit for retrieval was from the establishment of these databases to September 9, 2020. According to the inclusion and exclusion criteria, randomized controlled trials of ankle-foot orthoses in the treatment of children with cerebral palsy were included. At least two evaluators extracted the data independently and used Cochrane 5.1.0 bias risk assessment tool to evaluate the quality of included studies. The data were analyzed by Revman 5.3 software. RESULTS: In the end, a total of 12 randomized controlled trials with high quality, involving 480 children with cerebral palsy, were included in this study. Among them, 239 patients in the ankle-foot orthosis group wore ankle-foot orthosis; 241 patients in the control group did not wear ankle-foot orthosis. The treatment course was 0-48 weeks. Meta-analysis results showed that the improvement of Gross Motor Function Measure-88-D/E score (mean difference (MD)=6.26, 95% confidence interval (CI):3.54-8.98, P < 0.000 01), modified Ashworth scale score (MD=-0.60, 95%CI: -0.80 to -0.40, P < 0.000 01), dorsiflexion mean in swing (MD=14.96, 95%CI: 12.03-17.90, P < 0.000 01), effectiveness in improving motor function (MD=2.99, 95%CI: 1.19-7.50, P=0.02), and stride length (MD=0.06, 95%CI: 0.04-0.09, P < 0.000 01) in the ankle-foot orthosis group was better than that in the control group. CONCLUSION: Evidence from these 12 randomized controlled trials indicates that no matter walking with ankle-foot orthosis, or walking without ankle-foot orthosis after wearing for a certain period of time, in the short time, it has remarkable clinical value in improving motor function. However, further studies on the long-term recovery of motor function are required. [ABSTRACT FROM AUTHOR]

Copyright of Hydrogeology & Engineering Geology / Shuiwendizhi Gongchengdizhi is the property of Hydrogeology & Engineering Geology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Chinese Institute of Food Science & Technology is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of New Building Materials / Xinxing Jianzhu Cailiao is the property of New Building Materials Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The purpose of this study is to predict and analyze the structure and function of out membrane protein LC(OmpLC) of Edwardsiella ictaluri. The OmpLCgene was amplified by PCR method. The characteristics of physico-chemical parameters, hydropathy profile, signal peptide, transmembrane helices, secondary structure and tertiary structure were analyzed by bioinformatics softwares and web servers. The results showed that OmpLC protein of Edwardsiella ictaluri composed of 360 amino acids, the calculated molecular mass was 39.407 kD and the theoretical isoelectricpoint was 4.98, and the instability index was 18.26. OmpLC protein had signal peptide and strong hydrophilicity but without transmembrane helices in mature protein. In the second structure α-helix, β-sheet and random coil made up 6.67%, 45.28%, 48.06%, respectively. The three-dimensional structure was β-barrel, its main domain was classified to gram_neg_porins which belong to OM_channels superfamily. The multiple alignment and clustering analysis showed that OmpLCprotein of Edwardsiella ictalurihas high similarity to other OmpLC proteins (AEQ59632,AEQ59639).The properties of OmpLCobtained by bioinformatic analysis can provide reference for the prodiction of novel functional domain and the research on related pathogenesis and new vaccines. [ABSTRACT FROM AUTHOR]