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旨在为提高油橄榄冠层生产力及其树体的整形修剪、病虫害防治提供理论依据，以四川省凉山州主栽油橄榄品种克罗莱卡、科拉蒂、阿布桑娜、鄂植8号为研究对象，研究了4个品种油橄榄上、中、下冠层的果实表型性状、含油率、含水率，橄榄油酸度（以游离油酸含量计）、脂肪酸组成及相对含量变化情况，并采用主成分分析和熵权优劣解距离法（EW-TOPSIS）进行综合评价。结果表明：随着冠层部位由下至上，不同品种油橄榄单果质量、横径、鲜果含油率和不饱和脂肪酸相对含量呈上升趋势，而果形指数、硬度、酸度和饱和脂肪酸相对含量呈下降趋势，不同品种油橄榄鲜果含水率均为树体上部最低；主成分分析和EW-TOPSIS评价结果表明，同一品种冠层上部的综合评价值最大，中部次之，下部最小，其中鄂植8号上部在主成分分析中综合得分最高，科拉蒂上部在EW-TOPSIS中综合得分指数最高。综上，油橄榄树冠层上部的果实性状和品质优于中、下部，在整形修剪过程中，应对不同的冠层进行不同程度的整形修剪，通过改善通风透光性提高冠层生产力，防治病虫害。In order to provide theoretical basis for improving canopy position productivity and its tree shaping and pruning, disease and pest control, the main olive varieties Koroneiki, Coratina, Arbosana and Ezhi No. 8 in Liangshan Prefecture, Sichuan Province were used as the research objects, the changes of phenotypic traits, oil content, moisture content of fruit, and acidity (based on free oleic acid content), fatty acid composition and relative content of olive oil of four varieties of olives in the upper, middle and lower canopy were investigated, and a comprehensive evaluation was made using the principal component analysis method and Entropy Weight Technique for Order Preference by Similarity to Ideal Solution (EW-TOPSIS). The results showed that with the canopy position from the bottom to the top, different varieties of olive single mass, fruit transverse diameter, fresh fruit oil content and the relative content of unsaturated fatty acids showed an increasing trend, while the fruit shape index, fruit hardness, acidity and the relative content of saturated fatty acids showed a decreasing trend; the moisture content of fresh olive fruits of different varieties was the lowest in the upper position of the tree. The results of the principal component analysis and the evaluation of the EW-TOPSIS indicated that the upper canopy of the same variety had the highest composite score, followed by the middle one and the smallest one in the lower position, in which the upper position of Ezhi No. 8 had the highest composite score in the principal component analysis, and the upper position of Coratina had the highest composite score in the EW-TOPSIS. In conclusion, the fruit traits and quality of the upper position of olive tree are better than that of the middle and lower parts, and in the process of shaping and pruning, different degrees of shaping and pruning should be carried out on different canopy layers to improve the canopy productivity by improving ventilation and light transmission, and preventing pests and diseases.

随着装配式钢结构在建筑工程领域的应用日益广泛，单边螺栓连接作为一种高效、便捷的连接方式，逐渐受到业界的青睐。H型钢梁、方钢管柱通过T型钢连接件和单边螺栓连接技术形成新型装配式钢框架结构，梁柱节点作为整个框架体系的核心，其抗震性能对整体结构的影响至关重要。为评估单边螺栓T型钢连接梁柱节点在地震过程中的损伤情况，开展了4种不同刚度单边螺栓T型钢连接梁柱节点的拟静力试验。通过试验，获得了单边螺栓T型钢连接梁柱节点的破坏模式、滞回性能和初始转动刚度，分析了不同刚度的T型钢连接件对梁柱节点的破坏模式、抗弯承载能力和转动刚度退化的影响。采用考虑残余变形累积损伤修正的Park-Ang损伤指数模型研究了试验中节点的损伤演化规律，结果表明：所提出的地震损伤模型能够较好地反映单边螺栓T型钢连接梁柱节点的损伤演化过程与破坏程度，为该类梁柱连接节点的优化设计和安全评估提供了理论依据和实践经验。

Objective To investigate the knowledge and practice regarding disposal of waste insulin needles in patients with diabetes mellitus. Methods Using self-designed questionnaires to investigate the knowledge and practice regarding disposal of waste insulin needles in 307 inpatients with diabetes mellitus. Results The total scores of disposal of insulin needles was(6. 60±1. 51). Univariate analysis showed that history of diabetes、history of insulin injection、receiving instruction of disposal of insulin needles were related with knowledge of disposal of insulin needles(P＜0. 05). 38. 11%(117/307)of patients recapped and threw the used insulin needles into waste containers and brought to hospital when returning hospital. 25. 08%(77/307) of patients recapped and threw into special waste containers. Conclusion The behaviors of disposing used insulin needles was not optimistic and medical staff should strengthen the education of disposing waste insulin needles, and promote the management of the disposal. (目的 调查废弃胰岛素针头处理相关知识和行为的现状。方法 采用自行设计的废弃胰岛素针头处理知识和行为问卷对307例住院糖尿病患者调查。结果 废弃胰岛素针头处理知识总分为(6. 60±1. 51)分。单因素分析结果显示, 糖尿病病史、胰岛素注射史时长、是否接受过废弃胰岛素针头处理的教育与废弃胰岛素针头处理知识有关(P＜0. 05)。38. 11%(117/307)的患者将废弃胰岛素针头盖好外针帽放进废弃容器内, 复诊时带回医院统一处理。25. 08%(77/307)的患者盖外针帽放进专用废弃容器内再丢进垃圾桶。结论 糖尿病患者废弃胰岛素针头处理行为方面不容乐观, 医护人员应加强废弃胰岛素针头处理的教育, 促进对废弃胰岛素针头的妥善管理。)

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Objective: To investigate the effect and mechanism of phyllanthus emblica extract on proliferation, migration and invasion of lung cancer cell A549. Methods: A549 cells were cultured in vitro and divided into control group, different doses (low, medium and high doses ) phyllanthus emblica extract group,si-NC group, si-LINC01772 group, high dose phyllanthus emblica extract+pcDNA group and high dose phyllanthus emblica extract+pcDNA-LINC01772 group. Cell proliferation was detected by cell counting kit-8 (CCK-8) and clone formation assay. Cell migration was detected by scratch assay. Cell invasion was detected by Transwell assay.The expression levels of E-cadherin and N-cadherin in the cells were detected by Western Blot, and the expression levels of LINC01772 and miR-153 were detected by real-time fluorescence quantitative PCR (RT-qPCR). The regulatory relationship between LINC01772 and miR-153 was verified through the dual luciferase reporter gene assay. Results: Compared with the control group, the expression of LINC01772 in A549 cells in different doses of phyllanthus emblica extract groups was decreased, and the optical density (OD value), number of clone formation, migration and invasion cells were decreased (P<0.05), while the content of miR-153 and the expression of E-cadherin protein were increased (P<0.05), with a dose-dependent manner (P<0.05). LINC01772 negatively regulated miR-153 expression in A549 cells. Compared with the si-NC group, the proliferation, invasion and migration of A549 cells in the si-LINC01772 group were inhibited (P<0.05). Compared with the high dose phyllanthus emblica extract+pcDNA group, the proliferation, invasion and migration of A549 cells in the high dose phyllanthus emblica extract+pcDNA-LINC01772 group were enhanced (P<0.05). Conclusion: Phyllanthus emblica extract may inhibit the proliferation, migration and invasion of lung cancer cell A549 by regulating the LINC01772/miR-153 axis, which may play a role by down-regulating LINC01772 and up-regulating the expression of miR-153, and has the potential value to be developed as a drug for the treatment of lung cancer. [ABSTRACT FROM AUTHOR]

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[Objective] To determine the amount of biochar in the cultivation substrate and use biochar to replace the use of a scarce resource.grass carbon. [Method] The composite matrix was formed by mixing corn straw charcoal (biochar), peat, organic fertilizer and active bacteria in four different ratios (CK, 8 : 1.5 : 0.5) (treatment①, 7 : 1 : 1.5 : 0.5) (treatment ②, 6 : 2 : 1 : 5 : 0.5) (treatment ③, 5 : 3 : 1.5 : 0.5). The seedling traits, water content, chlorophyll content, crude protein, reducing sugar and nitrogen, phosphorus and potassium content were determined. [Result] In terms of rapeseed growth, treatment ① (10% biochar supplemental) had the best growth, plant height, diameter and leaf size grew more neatly; the content of chlorophyll in treatment ① (10% biochar content) was the highest (1.37 g/kg), which was significantly higher than that in CK group; the crude protein content of the four treatments was relatively high, the crude protein content of treatment ① and ② was higher than that of CK group, and the crude protein content of treatment ③ (30% biochar addition) was slightly lower than that of control group; the reducing sugar content of each treatment decreased significantly with the increase of biochar application amount, and the reducing sugar content of control group was the lowest, and that of treatment ① was the highest, which was 68% higher than that of control group; the content of P in all treatments was lower, the content of N and K was relatively higher, and the content of nitrogen, phosphorus and potassium in rape was not significantly different. [Conclusion] The matrix formula of 70% peat + 10% biochar + 15% organic fertilizer + 5% active bacteria has the best agricultural effect. Application of 10% biochar in the substrate is ideal, and a small amount of peat can be replaced in production and life. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Henan Agricultural Sciences is the property of Editorial Board of Journal of Henan Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To observe the effects of analgesia after surgery with esketamine combined with sulfentanyl on cellular immunity function, pain factors and cognitive function in elderly patients with laparoscope colon cancer radical surgery. Methods: 156 elderly patients with laparoscope colon cancer radical surgery who were admitted to Tianyou Hospital Affiliated to Wuhan University of Science and Technology from January 2021 to October 2022 were included. The patients were divided into control group (analgesia after surgery with sulfentanyl citrate injection) and study group (analgesia after surgery with esketamine hydrochloride injection combined with sulfentanyl citrate injection for analgesia after surgery), 78 cases in each group. The effective number of compressions for intravenous patient-controlled analgesia pump (PCIA), Ramsay sedation score, pain visual analogue score (VAS), cellular immunity function, pain factors, cognitive function and the incidence of adverse reactions were compared between two groups. Results: The number of effective PCIA compressions in study group was less than that in control group (P＜0.05). 24 h after surgery and 48 h after surgery, the VAS score in study group was lower than that in control group, Ramsay sedation score was higher than control group (P＜0.05). 48 h after surgery, The β-endorphin (β-EP) in study group was higher than that in control group, substance P (SP) and prostaglandin E2 (PEG2) were lower than those in control group (P＜0.05). 48h after surgery, CD8+ in study group was lower than that in control group, CD3+, CD4+ and CD4+/CD8+ were higher than those in control group (P＜0.05). The MMSE scores in study group at 12 h after surgery, 24 h after surgery and 48 h after surgery were higher than those in control group (P＜0.05). There was no significant difference in the incidence of adverse reactions between two groups (P＞0.05). Conclusion: Analgesia after surgery with esketamine combined with sulfentanyl in elderly patients with laparoscope colon cancer radical surgery can obtain good sedative and analgesic effects, can reduce the number of effective PCIA compressions, relieve the immunosuppression, reduce the excessive secretion of pain factors, and improve cognitive function. [ABSTRACT FROM AUTHOR]

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Most white blood cells (WBCs) recognition models present the disadvantages such as limited deployment due to large parameter count and computation amount, low WBC recognition accuracy, and poor generalization ability. Therefore, a lightweight and efficient WBCs recognition model based on improved EfficientNet is proposed. Firstly, the main modules are streamlined to reduce the model parameter count, while jump connections between feature layers are added to ensure a complete information flow. Secondly, the main module is optimized by adding the improved efficient channel attention and selecting a more suitable DropBlock2D. The improved module makes the model capture more channels and detail features, thus improving the recognition accuracy and generalization ability. Finally, the model is trained by a cross-entropy loss function with label smoothing to accelerate the convergence of the model and further enhance the generalization ability further. The experimental results show that the number of parameters of the improved model is 2.49M, which is 1.11M less than that before the improvement, simplifying the complexity of the model. The improved model achieves 99.67% accuracy in the classification task for the mixed dataset, which is 0.37% better than before the improvement. In addition, the model achieves 100.00% accuracy in the classification of the public dataset BCCD2, which is higher than the existing WBCs recognition models, verifying that the model has high accuracy and good generalization ability while maintaining lightweight computation. [ABSTRACT FROM AUTHOR]

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Objective: To investigate the effects of esmolol hydrochloride combine with dexmedetomidine on stress response and helper T cell 1 (Th1) /Th2 cytokines in lung cancer patients. Methods: A total of 112 patients with lung cancer undergoing radical surgery who were admitted to Tianyou Hospital affiliated with Wuhan University of Science and Technology from June 2020 to March 2022were selected. The patients were divided into control group (n=56, conventional anesthesia combined with dexmedetomidine hydrochloride injection) and study group (n=56, control group combined with esmolol hydrochloride injection) according to the random number table method. Awakening quality, lung function indicators, stress response indicators, Th1/Th2 cytokines, and postoperative pulmonary complications between the two groups were compared. Results: The respiratory recovery time, directional recovery time, and extubation time of the study group were shorter than those of the control group (P＜0.05) . The incidence of postoperative pulmonary complications in the study group was lower than that in the control group (P＜0.05) . The postoperative dynamic lung compliance (Cdyn) of the study group after operation was higher than that of the control group, while platform pressure (Pplat) and peak airway pressure (Ppeak) were lower than those of the control group (P＜0.05) . The postoperative cortisol (Cor), norepinephrine (NE), and epinephrine (E) levels in the study group after operation were lower than those in the control group (P＜0.05) . Tumor Necrosis Factor-α (TNF-α), interleukin-2 (IL-2) of the study group after operation were lower than those of the control group (P＜0.05), Interleukin-10 (IL-10), transforming growth factor-β1 (TGF-β1) of the study group after operation were higher than those of the control group (P＜0.05) . Conclusion: Esmolol hydrochloride combine with dexmedetomidine has a good anesthetic effect in radical lung cancer surgery, which can effectively reduce intraoperative stress response, regulate immune function, and protect lung function. It can also reduce the incidence of postoperative pulmonary complications. [ABSTRACT FROM AUTHOR]

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