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目的探讨非免疫性胎儿水肿（NIHF）的超声特征及病因学分布情况。方法纳入2012年12月至2019年1月就诊于中山大学附属第一医院产前超声诊断为NIHF病例232例，回顾性分析其超声特征及病因学分布情况。结果① NIHF最常见于TTTS Ⅳ期（50/232，21.55%）；最常见超声水肿的部位为皮肤水肿（159/232，68.53%）；最常合并的畸形为心血管系统异常（15/232，6.47%）。NIHF的活产率为31.47%。② 185例行产前遗传学检测的病例中，异常检出率40.54%（75/185），行染色体检查（包括染色体核型分析、染色体微阵列分析）的异常检出率26.49%（49/185），孤立性NIHF的异常检出率低于非孤立性NIHF的异常检出率（32.64% vs. 68.29%，P＜0.05 ）。仅行染色体核型分析的异常检出率24.56%（14/57）；仅行染色体微阵列分析（CMA）的异常检出率41.94%（13/31）；同时行染色体核型分析及CMA的异常检出率25%（22/88），其中染色体核型分析检出异常3例（3.41%），CMA检出异常6例（6.82%），CMA较染色体核型分析额外检出3例（3.41%），染色体核型分析及CMA均检出变异13例（14.77%）。本研究中染色体核型分析异常检出率20.69%（30/145），最常见的染色体异常是45， X（19/145，占13.10%），其次是47， XX， +21/47， XY， +21（3/145，2.07%），染色体微阵列分析异常检出率26.89%（32/119），其中拷贝数变异（CNVs）占10.08%（12/119），包括致病性CNVs 4例（3.36%，4/119）。行基因检测病例中最常见异常是α地中海贫血--SEA/--SEA 21例（21/26， 80.77%），其次是PTPN11基因突变2例（2/26，7.69%）。③ 遗传学未检出异常的NIHF病因有胎母输血、感染及不明原因的贫血。结论本中心NIHF最常见于TTTS Ⅳ期，最常合并的畸形为心血管系统异常，最常见的异常染色体核型是45， X；CMA较染色体核型分析额外检出率3.41%；最常见的单基因病是α地中海贫血--SEA/--SEA。非孤立性NIHF的遗传学异常检出率更高。

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Metabolic associated fatty liver disease (MAFLD) is currently the most common liver disease around the world. MAFLD may easily progress to the adverse outcomes such as hepatitis, liver fibrosis, and liver cirrhosis, and it is often accompanied by comorbidities such as diabetes, hypertension, hyperlipidemia, hyperuricemia, and cardiovascular and cerebrovascular diseases. Etiological treatment is the cornerstone of MAFLD treatment, and due to the complexity and adverse outcome of MAFLD, it is of great significance to explore the pathogenesis of MAFLD and develop effective prevention and treatment regimens and drugs. This article reviews the pathogenesis of MAFLD from the aspects of genetic factors, improper diet and oxidative stress, spleen-stomach damp-heat and insulin resistance, damp-heat and pathogenic Qi, organic acid metabolism, and intestinal microecology. [ABSTRACT FROM AUTHOR]

BACKGROUND: As a common clinical digestive disorder, irritable bowel syndrome becomes an advantageous disease of acupuncture treatment. However, the therapeutic mechanisms remain unclear. The methodological characteristics of omics coincide with the multi-target and multi-level characteristics of acupuncture, providing the possibility of revealing the principle of acupuncture in the treatment of the disease. OBJECTIVE: To investigate the pathogenesis of irritable bowel syndrome with diarrhea (IBS-D) and the effect of acupuncture at the combined points (selected based on etiologies and symptoms) on IBS-D based on proteomics. METHODS: Twelve 3-month-old male Sprague-Dawley rats were randomly divided into three groups: a control group, an IBS-D model group and an acupuncture group. The IBS-D rat models were prepared using the CAS method. After successful modeling, bilateral Zusanli points, bilateral Neiguan points and Guanyuan points were selected for acupuncture treatment in the acupuncture group, with a frequency of 120 times/minute, 1 minute of acupuncture every 4 minutes, and 15 minutes of needle retention, at an interval of 1 day every 6 days, for 28 days in total. Rats in the normal control group and the model group were not given any intervention. The pressure threshold of rat abdominal retraction reflex was measured to evaluate the visceral hypersensitivity of rats. Proteomics analysis was performed using the liquid chromatography-tandem mass spectrometry -based platform. MaxQuant software, Perseus software and DAVID, KOBAS, VENNY, STRING online tools were used for the bioinformatics analysis of proteomic data. Visualization analysis was done using Cytoscape 3.7.1 software. RESULTS AND CONCLUSION: There were 47 differentially expressed proteins between the IBS-D model and control groups. Function analysis of differentially expressed proteins revealed that the pathogenic mechanism of IBS-D was associated with abnormal energy metabolism, the imbalance of colon motor function and increased visceral sensitivity. Important proteins related to IBS-D pathogenesis included Atp5a1, Atp5c1, Idh3b, Atp2a3, Pdhb, Ppp1ca and Mapk3. Sixtyone differentially expressed proteins were identified between the acupuncture group and IBS-D model group. Acupuncture at the combined points reversed the up-regulation of nine differentially expressed proteins and the down-regulation of nine differentially expressed proteins. Bioinformatics analysis revealed that acupuncture at the combined points for IBS-D could function via multi-targets and multi-pathways, reverse the damage of energy metabolism caused by IBS-D, and play a role against oxidative stress and inflammation, thereby relieving pain and regulating the imbalance of intestinal function. Important proteins related to acupuncture effects included Atp5a1, Atp5c1, Pdhb, Sars, Uqcrc2, Prdx2, Prdx4, Ppp1ca, Manf and Tmsb4x3. All these findings preliminarily illustrate the potential molecular mechanisms of IBS-D and the effect of acupuncture at the combined points in the treatment of IBS-D at the protein level, which provide a basis for the clinical application of acupuncture at the combined points. [ABSTRACT FROM AUTHOR]

In organic production and certification, identifying the application of chemically nitrogen (N) fertilizers is a priority issue as it is impossible to differentiate organic and chemical N during the final agricultural product analysis. This study chose five organic tea gardens (ZX, HLS, LS, YS, and YCT) in Pu′ er City, Yunnan Province, to explore and assess the risk of chemical fertilizer application in organic tea production of 2022. Based on the principle of N balance of the soil-crop system, and in combination with the supply of N from nonfertilizer sources, such as green manure, straw incorporation, and soils, and with consideration of the N uptake from tea harvest yield, the study estimated the N fertilization quantity to meet with the tea production, and calculated the N satisfaction rate to assess the risk of chemical N fertilization risk in organic tea plantation. The results showed that the annual tea yields of ZX, LS and YCT tea gardens were low, and the N requirement was 53.6, 43.1 kg·hm-2, and 27.4 kg·hm-2, respectively, the non-fertilizer N supplies ranged from 93.8 kg·hm-2 to 128.2 kg·hm-2, which were able to satisfy the tea production. The two tea gardens, i.e., HLS and YS, had high tea yields, and their nonfertilizer N supplies could not satisfy the tea production. The YS tea garden applied an additional 63.0 kg·hm-2 (calculated in N) from organic fertilizer complying with organic standards, which supplied more than 50% of the theoretical required fertilizer N input. HLS tea garden had high risk of chemical fertilizer N application, which was not in accordance with Chinese organic agriculture standard. The study shows that the actual fertilization of the 5 tea gardens studied is in high consistency with the assessment results, and the N satisfaction rate proposed in this study is based on the principle of N balance, which can well assess the technical compliance of fertilizer N use. In organic tea plantation production, the improvement of soil fertility through the continuous implementation of green manure, straw return to the field and other measures is an important guarantee to ensure the sustainability of tea plantation production and compliance with organic certification, and should be further verified and promoted for application in organic agriculture. [ABSTRACT FROM AUTHOR]

Objective To investigate potential causative associations between immunophenotype traits and cervical cancer by using two-sample Mendelian randomization (MR) analysis. Methods The genetic instrumental variables (IVs) of 731 immunophenotypes of peripheral blood were obtained from the GWAS Catalog database. The GWAS summary data of cervical cancer were obtained from FinnGen database. The inverse-variance weighted (IVW), weighted mode, weighted median, and MR Egger methods were used for evaluations. The sensitivity analysis and reverse Mendelian randomization analysis were conducted to eliminate bias and reverse causality. The MR Steiger directionality test was further used to ascertain the reverse causal relationship between immune cells and cervical cancer. Results A total of 71 immune cell subtypes associated with cervical cancer were identified, of which 31 had a strong association. The majority of the B cell panel was protective factors for cervical cancer. B-cell activating factor receptor (BAFF-R) was the most frequently expressed molecule in this analysis. It is expressed on several B cell subtypes. The CD20 on IgD+ CD38+ B cell (OR=1.887, 95%CI: 1.078-3.306, P=0.026) is the risk factor for cervical cancer. In cDC panels, the CD123 expression on plasmacytoid dendritic cell (OR=2.48, 95%CI: 1.229-5.003, P=0.011), CD123 expression on CD62L+ plasmacytoid dendritic cell (OR=2.5, 95%CI: 1.231-5.077, P=0.011), CD80 expression on plasmacytoid dendritic cell (OR=2.62, 95%CI:1.244-5.515, P=0.011), and CD80 expression on CD62L+ plasmacytoid dendritic cell (OR=2.641, 95%CI: 1.246-5.596, P=0.011) were positively associated with the incidence of cervical cancer. All gynecological cancers in this study have no statistically significant effect on immune cells, according to reverse MR analysis. Conclusion This study emphasized the genetically predicted causality between immune cells and cervical cancer. In clinical practice, it is important to pay attention to the screening of peripheral blood immune cells for patients with cervical cancer. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Jilin University (Science Edition) / Jilin Daxue Xuebao (Lixue Ban) is the property of Zhongguo Xue shu qi Kan (Guang Pan Ban) Dian zi Za zhi She and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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BACKGROUND: In recent years, a variety of lasers have been widely used in various diseases related to stomatology, including the prevention and treatment of dental caries. OBJECTIVE: To investigate the effect of neodymium-doped:yttrium aluminum perovskite (Nd:YAP) laser combined with two remineralizers on early enamel caries in vitro. METHODS: Early enamel caries models in vitro were artificially established by 60 enamel blocks and randomly divided into 6 groups (n=10). Group A did not undergo any treatment but underwent extracorporeal pH circulation. Group B underwent remineralization of dentin (the main component of casein phosphopeptide-amorphous calcium phosphate composite) and extracorporeal pH circulation. Group C underwent remineralization treatment of Sensodyne toothpaste (the main component of bioactive glass) and then underwent extracorporeal pH circulation. Group D received Nd:YAP laser irradiation and extracorporeal pH circulation. Group E was treated with Nd:YAP laser irradiation, with remineralization of dentin, and then with extracorporeal pH circulation. In group F, Nd:YAP laser irradiation was performed, and then Sensodyne toothpaste was used for remineralization, and the extracorporeal pH circulation was performed; the remineralization treatment was conducted twice a day, and the experimental period was 20 days. Group G was a normal control group, without caries or remineralization, but only underwent extracorporeal pH circulation. After the experiment, the microhardness, morphology and Ca/P ratio of the dental enamel surface were measured in each group. RESULTS AND CONCLUSION: (1) The surface microhardness value of dental enamel in groups B, C and D was higher than that in group A (P < 0.000 1); the surface microhardness value of dental enamel in groups E and F was significantly higher than that in groups B, C and D (P < 0.000 1), and the surface microhardness value of dental enamel in group F was significantly higher than that in group E (P < 0.000 1). (2) Scanning electron microscopy showed that there were a lot of demineralized pores on the enamel surface of group A. There were mineral deposits on the enamel surface of group B, which were uneven and loose. In group C, there were a lot of mineral deposits on the enamel surface, and demineralized pores were found between the calcified masses. The enamel surface of group D was relatively flat; the demineralized pores were significantly smaller than that of group A, and the enamel column interstitium was damaged. In group E, the mineral deposits on the enamel surface were thicker and the demineralized pores were significantly reduced. The mineralized substances deposited on the enamel surface of group F were most dense and uniform and the demineralized pores were small. (3) The Ca/P ratio on the enamel surface of groups B and C was significantly higher than that of group A (P < 0.000 1); the Ca/P ratio on the enamel surface of group E was significantly higher than that of groups B, C and D (P < 0.000 1), and the Ca/P ratio on the enamel surface of group F was higher than that of group E (P < 0.001). (4) These findings indicate that bioactive glass, casein phosphopeptide-amorphous calcium phosphate composite, and Nd:YAP laser after enamel demineralization can promote the remineralization of early enamel caries. Nd:YAP laser combined with bioactive glass or casein phosphopeptide-amorphous calcium phosphate composite can further strengthen the remineralization of dental enamel caries, and the combination of Nd:YAP laser and bioactive glass has the best effect. [ABSTRACT FROM AUTHOR]

Objective To construct mouse BaF3-FIP1L1-PDGFRA (F/P), BaF3-F/P-T674I and BAF3-F/P-D842V pre-B cell strains which stably express F/P fusion protein and its T674I and D842V mutants in order to evaluate their activity by checking their responses to tyrosine kinase inhibitors (TKIs). Methods Lentivirus infected technique was used to transfect the target gene into BaF3 cells. RT-qPCR was used to detect mRNA expression, and CCK-8 method was used to detect the inhibitory effect of TKIs on the proliferation of stable cell strains. Results The constructed BaF3-F/P, BaF3-F/P-T674I and BAF3-F/P-D842V cell strains all transcripted FIP1L1 and PDGFRA mRNA. They exhibited malignant phenotypic characteristics of proliferation independent of IL-3 and sensitivity to corresponding TKIs. Conclusions The pre-B-cell strains stably expressing F/P and its T674I and D842V mutants are successfully constructed, which provide a good cell model for the development of compounds targeting at those molecules. [ABSTRACT FROM AUTHOR]

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Objective　To investigate the supportive care needs of patients after radical surgery for breast cancer based on the Kano model and define their attributes. Methods　Convenience sampling was used to select 210 patients who underwent radical surgery for breast cancer and were hospitalized in a tertiary cancer specialty hospital in Yunnan Province from June 2021 to December 2021. The Kano model, Supportive Care Needs SurveyShort Form 34（SCNS-SF34), and Better-Worse two-dimensional matrix were used to classify patients' supportive care needs. Furthermore, factor selection lines and sensitivity screening were applied to identify the needs that require significant improvement and rank them. Results　A total of 210 questionnaires were distributed, and 200 valid questionnaires were collected, with an effective response rate of 95.2%. There were 2 mandatory attribute needs（5.9%), 4 desired attribute needs（11.8%), 23 attractive attribute needs（67.6%), and 5 indifferent attribute needs（14.7%）. Fifteen needs were identified as priorities for improvement, with the highest sensitivity being 0.228 and the lowest being 0.012. Conclusion　Healthcare professionals should continuously improve and optimize supportive care measures based on patients’ different needs to enhance their healthcare experience. [ABSTRACT FROM AUTHOR]

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OBJECTIVE: To systematically evaluate the efficacy and safety of Yiguanjian decoction combined with conventional western medicine in the treatment of ascites induced by liver cirrhosis. METHODS: CNKI, VIP, Wanfang Data, Embase, Web of science, PubMed and Clinical Trials databases were retrieved to collect randomized controlled trials that met the screening criteria (the control group received conventional western medicine, while the observation group received Yiguanjian decoction on the basis of control group ), the search time was from the establishment of the database until Feb. 2023. The Cochrane Collaboration Network bias risk assessment was used for quality assessment, and Meta-analysis was performed by using RevMan 5. 4 software. RESULTS: Totally 15 articles were enrolled, including 1 147 patients. Meta-analysis showed that compared with the control group, the treatment regimen in the observation group could improve the total effective rate (RR = 1. 31,95%CI = 1. 24-1. 40,P<0. 000 01) and reduce abdominal circumference (MD = - 8. 26,95% CI = - 10. 44-- 6. 09,P < 0. 000 01), decrease body mass (MD= -6. 86,95%CI = -8. 23--5. 48,P<0. 000 01), increase 24 h urine volume (MD = 465. 38,95%CI = 351. 27- 579. 48,P<0. 000 01), and shorten disappearance time of ascites (MD= -7. 29,95%CI = -13. 97--0. 61,P = 0. 03), and improve liver function. Meanwhile, the incidence of adverse drug reactions in the observation group decreased. CONCLUSIONS: Yiguanjian decoction can improve the efficacy of conventional western medicine treatment of cirrhotic ascites, with few adverse drug reactions. [ABSTRACT FROM AUTHOR]

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