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针对U型钢-混凝土组合梁交叉节点处U型钢腹板传力路径中断、节点受力复杂、现有钢筋过渡节点现场焊接量大的难题，提出了L形连接板传递钢腹板内力的新型节点（LLTC），对节点抗弯性能进行了试验研究。LLTC由套筒、L形连接板和套丝钢筋组成，与焊接钢筋型纵向过渡钢筋（WLTR）传递钢腹板内力的传统梁交叉节点（WLTR-UCJs）相比，LLTC能够有效传递钢腹板内力，且实现了节点的装配式连接。通过四端简支静力试验，研究了WLTR-UCJs和装配连接型U型钢-混凝土组合梁交叉节点（LLTC-UCJs）的抗弯性能，明晰了纵向传力构件形式、WLTR直径、LLTC套丝钢数量和LLTC套丝钢强度等对节点承载能力与变形能力的影响。试验结果表明，WLTR-UCJs的破坏模式为钢腹板屈曲和顶部纵向钢筋屈曲，LLTC-UCJs的破坏模式为U型钢翼缘屈服。与WLTR-UCJs相比，LLTC-UCJs的受压区材料屈曲程度较低，具有更高的承载力和更强的变形能力。

为获得折叠装配式框架结构的静力性能，设计了两个折展框架榀足尺试件，通过开展结构静力加载试验对单榀折展框架在不同荷载工况下的变形特征、承载性能与破坏形态进行了研究。试验结果表明：对于正常使用状态，进行静力加载时，有支撑的单榀框架结构整体变形处于弹性范围，水平作用下受推和受拉位移变化趋势基本一致，且增设支撑对折展框架的刚度影响较大；水平极限荷载作用下，有支撑的单榀框架结构在支撑连接处首先出现不可逆的塑性变形，单榀框架在边柱下端与下部拉条连接处发生局部破坏；上、下层梁柱节点弯矩-转角曲线变化趋势一致，但上层节点初始刚度明显弱于下层节点初始刚度，同层梁柱节点初始刚度基本相同；单榀框架的各关键点处等效应力在往复加载过程中推拉对称，结构总体处于安全状态。

为获得折叠装配式框架结构的静力性能，建立两组折展框架榀足尺试验模型，通过开展结构的静力加载试验对单榀折展框架在不同荷载工况下的变形特征、承载性能与破坏形态进行了研究。试验结果表明：正常使用阶段静力加载时，有支撑的单榀框架结构整体变形处于弹性范围，水平作用下受推和受拉位移变化趋势基本一致，且增设支撑对折展框架的刚度影响较大；水平极限荷载作用下，有支撑的单榀框架结构在支撑连接处首先出现不可逆的塑性变形，单榀框架在边柱下端与下部拉条连接处发生局部破坏；上、下层梁柱节点弯矩-转角曲线变化趋势一致，但上层节点初始刚度明显弱于下层节点，同层梁柱节点初始刚度基本相同；单榀框架的各关键点处等效应力在往复加载过程中推拉对称，结构总体处于安全状态。

目的探讨糖代谢异常的Ⅰ期子宫内膜样腺癌（EA）患者预后差异及其影响因素。方法选取2015年1月至2019年12月期间在中山大学孙逸仙纪念医院妇科肿瘤专科行手术治疗的Ⅰ期EA患者共387例，根据糖代谢是否正常分为糖代谢异常（IGM）组和糖耐量正常（NGT）组。其中IGM组分三个亚组，即糖尿病（DM）组、糖耐量异常（IGT）组、空腹血糖受损（IFG）组。回顾性分析各组临床病理指标及预后，探讨糖代谢异常对Ⅰ期子宫内膜样腺癌患者预后的影响及影响预后的相关因素。结果IGM组患者的5年生存率及5年无进展生存率均低于NGT组（P＜0.05）。DM组中血糖控制不达标者（糖化血红蛋白≥7.0%）5年生存率及5年无进展生存率均低于血糖控制达标者（P＜0.05）。多因素COX回归分析提示，CA125水平（≥ 35 U/mL）、淋巴脉管间隙浸润（LVSI）阳性是5年生存率的独立危险因素；脂代谢异常、LVSI阳性、深肌层浸润是5年无进展生存率的独立危险因素。结论Ⅰ期子宫内膜样腺癌患者中合并糖代谢异常者较糖耐量正常者预后差，血糖控制良好可改善合并糖尿病的早期子宫内膜样腺癌患者预后，CA125水平、LVSI阳性、脂代谢异常和深肌层浸润等因素可能影响Ⅰ期子宫内膜样腺癌患者的预后。

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With the rapid development of intensive culture of shrimp, the pursuit of high yield coupled with excessive feeding leads to the aggravation of NH4+-N, NO2-N) and sulfide pollution in the aquaculture water, which seriously harms the growth and physiological function of shrimp. This study used heterotrophic nitrification-aerobic denitrifying bacteria (HHVEN1 and SDVEA2) and sulfur-oxidizing bacteria (GHWS3 and GHWS5) screened from shrimp aquaculture environment to successfully construct two efficient denitrification/desulfurization bacterial consortia NS1-1 and NS2-2, and explored their denitrification and desulfurization capabilities. The results showed the NS1-1 consortium displayed removal rates exceeding 95% for NH4+-N, NO2-N), and sulfide, and NS2-2 exhibited removal rates of 90.27%, 97.38%, and 89.62%, respectively, all of which were superior to a single strain. The bacterial consortia NS1-1 and NS2-2 can exert maximum denitrification/desulfurization efficiency under the culture conditions of temperature 20-35 °C, carbon-nitrogen ratio (C/N) 5-20, salinity 20-30, and utilizing glucose or sodium succinate as the carbon source. The optimum pH ranges of the two consortia were different, with NS1-1 favoring 7.5-8.0 and NS2-2 preferring 7.0-8. 5. The bacterial consortia NS1-1 and NS2-2 had good coexistence among various strains in aquaculture tailwater. NS1-1 and NS2-2 displayed effective removal rates of 78.75%,75.09%,79.61%, and 81.44%,62.68%, 72.64% for NH4+-N, NO2-N)-and sulfide in practical aquaculture tailwater, respectively. The research shows that the bacterial consortia NS1-1 and NS2-2 have high-efficiency denitrification desulfurization removal efficiency and exceptional environmental adaptability, providing scientific support for eliminating nitrogen and sulfur pollution in aquaculture. [ABSTRACT FROM AUTHOR]

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The purpose of this study was to investigate the effect of FABP4 gene on lipid accumulation and migration of endometrial epithelial cells in sheep. Three FABP4 interference fragments were designed and ligated with pGBplv-U6-mcherry-puro to construct interference vectors. The empty plasmid was used as the control (NC group), and the three interference recombinant plasmids were FABP4-shRNA1, FABP4-shRNA2, and FABP4-shRNA3, respectively. The recombinant plasmids were identified by enzyme digestion. After plasmid transfection, RT-qPCR was used to identify the interference efficiency. Western Blot was used to further verify the interference effect of the vector. The vector was transfected into endometrial epithelial cells cultured in vitro. The accumulation of lipid droplets was detected by BODIPY method. The triglyceride content in the cells was detected by detection kit. RT-qPCR was used to detect genes related to lipid metabolism, proliferation and migration. Cell scratch test was used to detect cell migration. The results showed that all vectors can effectively interfere with the expression of the FABP4 gene, with the FABP4-shRNA2 vector showing the best interference effect. After interfering with the expression of the FABP4 gene in endometrial epithelial cells, compared with the NC group, the ability of cells to aggregate lipid droplets decreased, the content of triglycerides was reduced, the expression of mRNA, related to cellular lipid metabolism and proliferation and migration decreased, and the speed of scratch healing was reduced. After reducing the expression of the FABP4 gene in endometrial epithelial cells, the level of cellular lipid accumulation and the ability of cells to migrate decreased. The study indicates that the FABP4 gene may play an important role in the pregnancy of sheep and the normal development of embryos, and it is worth further in-depth study in breeding practice. [ABSTRACT FROM AUTHOR]

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Objective: To evaluate the feasibility of using the Chinese version of Addenbrooke's cognitive examination version Ⅲ (ACE-Ⅲ) on cognitive assessment in patients with cerebellar infarction. Method: Forty patients with isolated cerebellar infarction were collected in the department of neurology, Beijing Tiantan Hospital, along with 40 healthy controls were recruited matched by age and education. The clinical demographics was collected and the severity of ataxia and balance dysfunction was assessed. ACE-Ⅲ was used to evaluate cognitive function. The Cronbach's alpha and receiver operating characteristic (ROC) curve were used to assess the reliability and validity of ACE-Ⅲ respectively. Furthermore, the correlation of ACE-Ⅲ total score with age and education was examined by spearman analysis. Result: The total score of ACE-Ⅲ in patients' group and healthy controls was 88.3±6.0 and 93.1±3.0 respectively, showing a significant different (P<0.001). Compared with healthy group, patients with cerebellar infarction had lower performance in attention (P<0.001), language fluency (P<0.001) and visuo-spatial function (P=0.001). The overall Cronbach's alpha of ACE-Ⅲ was 0.58, and the area under curve was 0.79±0.05 (95% confidence interval: 0.69—0.89). According to Youden index, the optimal cut- off for ACE-Ⅲ was 88. Furthermore, spearman analysis showed cognitive function was significant negative associated with age in patients' group (r=-0.52, P<0.0001) and healthy controls (r=-0.67, P<0.001), and was significant positive associated with education in patients' group (r=0.74, P<0.01) and healthy controls (r=0.72, P<0.0001) Conclusion: ACE-Ⅲ scale is a good tool to detect cognitive dysfunction in patients with isolated cerebellar infarction. The total score of ACE-Ⅲ is associated with age and educational attainment. [ABSTRACT FROM AUTHOR]

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糖尿病脑病(DE)是糖尿病最常见的慢性并发症之一, 其发病机制目前尚未完全阐明, DE作为中枢神经系统的严重并发症正逐渐成为国内外研究的热点之一。近年来, 随着学者们对DE 的研究进展,DE的发病机制也愈渐明确, 且针对不同的发病机制研究了多种治疗方法。该文就目前 对DE的发病机制和治疗已有的文献及新进展进行综述, 以期为DE的早期防治提供参考. [ABSTRACT FROM AUTHOR]

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