In order to investigate the effect of extraction methods, including cold pressing method, soxhlet method and aqueous enzymatic method, on the quality of walnut oil, and explore the key chemical components affecting the antioxidant activity of walnut oil. The physicochemical properties, chemical compositions and antioxidant activities of the walnut oils extracted by the extraction methods mentioned above were determined. In addition, the antioxidant evaluation model of walnut oil was established through multiple linear regression analysis to analyze the key chemical components, affecting the antioxidant activity of walnut oil. The results showed that the three extraction methods had little effect on the content and compositions of fatty acids and glycerides in walnut oil, but had great influences on the contents of total phenols and flavonoids, physicochemical properties and antioxidant activities of the oils. According to the antioxidant evaluation model of walnut oil, linoleic acid and total phenols were the key chemical components affecting the antioxidant activity of walnut oil. In conclusion, among the three extraction methods, the quality of walnut oil extracted by cold pressing method is the best, followed by that obtained by aqueous enzyme method. However, the yield of walnut oil extracted by aqueous enzyme method was higher than that by cold pressing method. More importantly, aqueous enzyme method can simultaneously produce bioactive peptides during the process of oil extraction, so it is a extraction method with great potential for application. [ABSTRACT FROM AUTHOR]