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内毒素耐受是指脂多糖(LPS)刺激后,机体或细胞对后续刺激反应性降低的一种现象。Toll样受体(TLR)参与了LPS信号的跨膜转导。TLRs信号转导通路在内毒素耐受发生中发挥了重要作用,其通过改变信号蛋白和下游细胞因子的结构和功能,导致炎性因子释放减少。丝裂原活化蛋白激酶(MAPKs)及IL-1受体相关激酶(IRAK)家族成员、细胞膜表面的清道夫受体SR-A、TLR4同源体RP-105、细胞因子信号抑制物(SOCS)等均可参与TLRs信号转导通路的调控。miRNA作为小分子非编码RNA,通过作用于炎症信号传导通路调控内毒素耐受的发生发展,同时可调控免疫反应和炎性因子表达。关于miRNA在内毒素耐受调控中的作用的研究较多。在TLRs信号转导通路中产生调控作用的miRNA主要有miRNA-155、miRNA-146、miRNA-98、miRNA-125b和let-7e,具体作用机制有待进一步探索。 [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression changes and clinical significance of serum N-terminus of type Ⅰ collagen (NTx), C-terminus of type Ⅰ collagen (CTX), and osteocalcin (BGP) in postmenopausal osteoporosis patients. Methods: Sixty postmenopausal osteoporosis patients admitted to our hospital from August 2017 to August 2022 were selected as the study subjects and divided into the observation group, and another 60 postmenopausal healthy volunteers who came to our hospital for physical examination during the same period were selected as the control group. The expression levels of NTx, CTX and BGP were compared between the two groups, and subject work characteristic (ROC) curves were established to analyze the diagnostic efficacy of NTx, CTX and BGP on postmenopausal osteoporosis. 35 postmenopausal osteoporosis patients with obvious and significant symptom reduction, significant improvement in X-ray examination and significant increase in BMD values were divided into the good prognosis group, and the remaining 25patients who did not meet the above criteria were divided into the poor prognosis group. The clinical general conditions of the two groups were compared, and logistic regression was applied to analyze the prognostic predictive value of NTx, CTX and BGP on postmenopausal osteoporosis. Results: The comparison of NTx, CTX and BGP expression levels between the two groups of subjects was significantly different, with NTx and CTX higher in the observation group and BGP lower in the control group (P＜0.05) ; the diagnostic efficacy of the combination of NTx, CTX and BGP for postmenopausal osteoporosis was better than that of a single test (P＜0.05) ; the comparison of age,BMI, combined underlying disease, and Ca expression levels between the good prognosis group and the poor prognosis group was not significantly different (P＞0.05), There was no significant difference between the good prognosis group and the poor prognosis group in terms of age, BMI, combined underlying disease, disease duration, Ca expression level (P＞0.05), and significant difference between the good prognosis group and the poor prognosis group in terms of severity of disease, BMD T value, estradiol, serum NTx, CTX, BGP expression level (P＜0.05) ; logistic regression analysis showed that CTX and BGP were independent influences on poor prognosis of postmenopausal osteoporosis factors (P＜0.05) . Conclusion: The expression levels of type I collagen amino terminal and type Ⅰ collagen carboxyl terminal in serum of postmenopausal osteoporosis patients are higher than those of non osteoporosis groups, and osteocalcin is lower than that of non osteoporosis groups. The combination of the three can improve the diagnostic efficacy of postmenopausal osteoporosis.In addition, CTX and BGP are independent influencing factors for poor prognosis of postmenopausal osteoporosis. Higher levels of CTX and lower levels of BGP may indicate poor prognosis in patients. Therefore, timely improvement of treatment measures is necessary in clinical practice to improve the prognosis of such patients. [ABSTRACT FROM AUTHOR]

Objective: To investigate the relationship between serum tumor markers and pathological features of cervical cancer and the prediction of postoperative recurrence. Methods: 82 patients with cervical cancer who came to our hospital from January 2015 to December 2017 were selected as the observation group, and 50 healthy women who came to our hospital for physical examination during the same period were selected. The serum levels of CA125, CA153, CA199 and CEA in both groups were detected by electrochemical luminescence immunoassay. Patients in the observation group were followed up until December 2022. The serum CA125, CA153, CA199, CEA levels of the two groups were compared, and the relationship between the serum CA125, CA153, CA199, CEA levels and clinicopathological characteristics of the observation group was analyzed. The recurrence of patients in the observation group was analyzed after the postoperative follow-up, and the univariate and multivariate Cox regression results of the recurrence of patients after radical resection of cervical cancer were analyzed. Prognostic value of serum CA125, CA153, CA199 and CEA levels for recurrence of cervical cancer after radical resection. Results: The levels of serum CA125, CA153, CA199 and CEA in observation group were significantly higher than those in control group (P<0.05). The levels of serum CA125, CA153, CA199 and CEA among patients with cervical cancer at different FIGO stages, depth of interstitial invasion and presence of lymph node metastasis were statistically significant (P<0.05). The follow-up time of 82 patients was 13-60 months, and the median survival time was 39 months. By the end of December 2022, 18 of 82 patients (21.95%) had relapse after surgery. Univariate and multivariate Cox regression analysis showed that FIGO stage in stage Ⅱ A, interstitial infiltration depth ≥ 1/2, lymph node metastasis, CA125 ≥ 307.41U/mL, CA153 ≥ 185.89U/mL, CA199 ≥ 153.23U/mL, CEA≥ 30.15ng/mL are independent risk factors for postoperative recurrence of cervical cancer.ROC curve showed that the AUC value of CA125+CA153+CA199+CEA in predicting postoperative recurrence of cervical cancer was significantly higher than that of CA125, CA153, CA199 and CEA alone (P<0.05). Conclusion: Serum CA125, CA153, CA199 and CEA are highly expressed in patients with cervical cancer, which is related to the depth of interstitial infiltration, FIGO stage, lymph node metastasis and postoperative recurrence. The combination of the four can be used as a predictor of postoperative recurrence of cervical cancer. [ABSTRACT FROM AUTHOR]