Search

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Control Theory & Applications / Kongzhi Lilun Yu Yinyong is the property of Editorial Department of Control Theory & Applications and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Transportation Science & Technolgy is the property of Transportation Science & Technology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To clone human Thocl gene and construct the eukaryotic expression vector pThocl-DsRed2-N1 , to observe the co-localization of Thocl with voltage-gated chloride channel 3 (C1C-3) in human cervical cancer HeLa cells, and to analyze whether C1C-3 is involved in the cell transcription. Methods Thocl coding sequence was amplified by RT-PCR and cloned into the pDsRed2-Nl vector to construct the eukaryotic expression vector pThocl-DsRed2-Nl , which was then identified by PCR, enzyme digestion (Sac 1 and RamH 1 ) , and gene sequencing. HeLa cells in the logarithmic phase were divided into 3 groups: the transfection group, empty transfection group, and control group, which were transfected with pThocl -DsRed2-Nl plasmid, pDsRed2-Nl plasmid, and lipidosome by Lipofectamine 2000, respectively. At 48 h, the gene expression was observed under inverted fluorescence microscope. The co-localization of Thocl with C1C-3 was detected by immunofluorescence in the the transfection group. Results PCR results showed that there was a 2. 1 kb target band, and meanwhile, 4. 7 kb and 2. 1 kb bands were obtained by enzyme digestion. Sequencing proved that the sequence and splicing order of Thocl gene was matched with Genebank, suggesting that the fluorescent eukaryotic expression vector pThocl-DsRed2-Nl was constructed successfully. Overt red fluorescence was observed in the empty transfection group. The red fluorescence intensity of the transfection group was between those of the empty transfection group and the blank control group, suggesting that Thocl was expressed in HeLa cells. C1C-3 was localized both in the nucleus and cytoplasm, mainly in nucleus. At the same time, Thocl was localized in the nucleus only, and yellow fluorescence was observed after we merged C1C-3 with Thocl , indicating that C1C-3 and Thocl were co-located in the nucleus. Conclusion The eukaryotic expression vector pThocl-DsRed2-Nl is successfully constructed, Thocl co-localizes with C1C-3 in the nucleus, suggesting that C1C-3 may be involved in the cell transcription process. [ABSTRACT FROM AUTHOR]

The dynamic characteristics of variable damping paradrogue are researched. The dynamic response process of hose-drogue system at different paradrogue angles during aerial refueling is simulated. Results demonstrate that the position of hose-drogue system can be regulated by changing paradrogue angles to control variable paradrogue damping. [ABSTRACT FROM AUTHOR]

The longitudinal aerodynamic characteristics of transport aircraft at the large flap angle are studied by the numerical method, and the flow mechanisms for such flows are analyzed. The results show that the limited separate flow of horizontal tail at the large flap angle causes the dis-original elevation oscillation, and the blended area of tail and body has large adverse pressure gradient, where exists the partial separated flow. The flow characteristics in this area causes the abnormal elevation oscillation for the aircraft. The results are also validated by experiments and flight tests. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)