The aim of the study was to investigate the effect of Mongolian veterinarymedicine Babu-7 compound effective ingredient (BSEIC) on intestinal mucosal barrier related indexes of calves with diarrhea. Eighteen calves were randomly divided into healthy group (NC group), diarrhea group (EC group), antibiotic group (CIP group), BSEIC low dose group (BSEIC-L group), BSEIC medium dose group (BSEIC-M group), BSEIC high dose group (BSEIC-H group), with three calves in each group. The calves were dosed twice daily for 7 d. Except NC group, the calves in other five groups were given 2.50×1011 CFU/mL of pathogenic E. coli O1, 100 mL/head orally on the 5th day. The results showed that compared with EC group, the T-AOC, the content of IL-4 and IL-10, the activity of CAT, GSH-Px and SOD in duodenum of BSEIC-M group were significantly increased (P<0.05), the content of IL-2 was extremely increased (P<0.01), and the content of MDA was extremely decreased (P<0.01), the content of IFN-γ, TNF-α, TGF-β and IL-1β were significantly decreased (P<0.05), and the number of duodenal goblet cells was extremely increased (P<0.01). Compared with EC group, DAO activity and D-LA content in serum of calves in all treatment groups were significantly decreased (P<0.05). The study indicates that the compound effective ingredient of Mongolian veterinary medicine Babu-7 can regulate the immune responseof calves, protect the permeability of intestinal mucosa and strengthen the function of intestinal mucosal barrier. [ABSTRACT FROM AUTHOR]