In order to investigate the resistance of different mature leaves to tobacco brown spot, and also the efficacy of fungicides to this disease, the resistance of leaves with different maturities (mature, proper and immature) to tobacco brown spot was measured, and proper tobacco leaves and three kinds of maturity mixed tobacco leaves (mature, proper and immature) were selected as the evaluation object of disease index, and the field efficacy of 15 kinds of fungicides (12.5% Myclobutanil ME, 325 g/L Difenoconazole Azoxystrobin SC, 57.60% Cu (OH)2 WDG, 40% Dimethachlon WP, 8% Validamycin SL, 80% Bordeaux mixture WP, 50% Kresoxim-methyl WDG, 10% Difenoconazole WDG, 12% Difenoconazole Fluzoxamides SC, 25% Azoxystrobin SC, 400 g/L Cloflurfenazole Pyraclostrobin SC, 10% Polyoxin WP, 38% Pyraclostrobin Boscalid WDG, 200 g/L Pydiflumetofen Difenoconazole SC, 80% Mancozeb WP) against tobacco brown spot were evaluated by the increase of disease index. Results showed that highest resistance to brown spot was the immature leaves at upper position, followed by the proper mature leaves in the middle, and poorest resistance was the mature leaves at down position. Fifteen kinds of fungicides showed different field efficacy on tobacco brown spot. Results of the two investigation methods were relatively consistent in the field efficacy of 12 kinds of fungicides, such as 325 g/L Difenoconazole Azoxystrobin SC, 57.60% Cu(OH), WDG. 325 g/L Difenoconazole Azoxystrobin SC (the field efficacies were 100% and 85.64% at 5 days after treatment, and 57.14% and 64.92% at 10 days after treatment), 12% Difenoconazole Fluzoxamides SC (82.81% and 87.56%, 50% and 70.82%), 25% Azoxystrobin SC (100% and 81.75%, 50% and 53.93%), 38% Pyraclostrobin Boscalid WDG (100% and 89.18%, 77.78% and 58%), 200 g/L Pydiflumetofen Difenoconazole SC (86.74% and 92.84%, 92.86% and 81.79%) had better field efficacies. And 25% Azoxystrobin SC (56.46% and 54.95%) and 200 g/L Pydiflumetofen. Difenoconazole SC (60.90% and 55.95%) still had good field efficacies after 15 days of application. Above all, the field efficacies of 325 g/L Difenoconazole Azoxystrobin SC, 12% Difenoconazole Fluzoxamides SC, 25% Azoxystrobin SC, 38% Pyraclostrobin Boscalid WDG and 200 g/L Pydiflumetofen Difenoconazole SC against tobacco brown spot were much better, they could be used in the control of tobacco brown spot. [ABSTRACT FROM AUTHOR]