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Objective To study the relative expression and function of the nuclear transcription factor ZNF407 in melanoma and its influence and mechanism on the migration and invasion. Methods From January 2015 to January 2021, 42 cases of cutaneous malig- nant melanoma and 20 eases of pigmented nevus in Department of Dermatology, Chongqing Traditional Chinese Medicine Hospital were collected. We examined the expression of ZNF407 in melanoma cell lines and tissues through semi-quantitative polymerase chain Reaction (qRT-PCR) and westernblot. Pearson correlation analysis detection was used to analysis mRNA expression of ZNF407 and TGF-B-1. Melanoma cell line A375 was set as target cells, pcDNA3.1 (+) Flag-ZNF407 (experimental group) and pcDNA3.1 (+)-Flag-Vector (negative control group) were transfected, respectively. The non transfected group was the set as blank control group. Transwell assay was used to detect the effects of celluar migration and invasion; the effects of ZNF407 on the expression levels of epithelial mesenchymal transition (EMT) related mRNA were detected by semi quantitative PCR and real-time quantitative PCR. Westernblots were used to ex- plore the mechanisms of ZNF407 in epithelial-mesenchymal transition and the mechanisms of ZNF407 on TGF/B signaling pathway. Results ZNF407 protein and mRNA were downregulated in human malignant melanoma tissues compared with normal tissues and its mRNA was down-regulated in melanoma cell lines (P < 0.001) Compared with the control group, transwell migration assay showed that the number of cells in the ZNF407 group (116±17) was lower than that in the control group (265±27, P<0.01) and blank control group (259 ± 28, P < 0.01 ) . Transwell invasion assay showed: The number of invated cells in the experimental group (82 ± 14) was lower than that in the control group (215±31, P<0.01) and blank control group (209 ± 32, P<0.01). Westernblot showed that overexpression of ZNF407 resulted in downregulation of protein expression of Vimentin, N-cadherin, Twist, and MMP7; The protein expression of E-cadherin was upregulated (P < 0.001) ; RT-PCR and qRT-PCR showed that over-expression of ZNF407 mRNA resulted in downregulation of stem cell markers ABCG2, Snail2, and OCT4 mRNA expression in the experimental group (P < 0.001) Correlation analysis showed that ZNF407 and TGF-β1 mRNA expression was negatively correlated in melanoma tissues ( P = 0.033, r = - 0.714 ). And over-expressed of ZNF407 inhibited cell migration and invation by downregulating of TGF-β-1 pathways and its downstream target proteins p-Smad1, p-Smad2, p-Smad3, p-Smad4 and c-Myc. Conclusion The expression of ZNF407 protein and mRNA was down-regulated in melanoma, over-expressed of ZNF407 expression could inhibit the cell migration and invasion of melanoma cell lines. Overexpression of ZNF407 could inhibit the ex- pression of TGF-β/Smad signal and its downstream of the pathway, and it could be acted as an important tumor suppressor gene in primary melanoma. [ABSTRACT FROM AUTHOR]

Objective To explore the application effect of functional dressings in the nursing of pressure injuries, and to provide evidence-based basis for the nursing of pressure injuries. Methods Computer search for randomized controlled trials on the effects of functional dressings in nursing of pressure injury in the Cochrane Library, PubMed, WOS, CNKI, Wanfang Data and VIP databases, screened and included the literature, and the search time limit was from the establishment of the database to June 2020. Two reviewers independently conducted literature screening, data extraction, and quality evaluation in accordance with Cochrane quality evaluation standards, and then used RevMan 5. 3 software for Meta analysis. Results Totally 17 articles were included, with a total of 1206 patients. The results of Meta analysis showed that functional dressings were superior to the control group in terms of nursing satisfaction, healing status, and healing time except for the number of dressing changes (P

Copyright of Acta Materiae Compositae Sinica is the property of Acta Materiea Compositae Sinica Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chinese Journal of Computational Mechanics / Jisuan Lixue Xuebao is the property of Chinese Journal of Computational Mechanics Editorial Office, Dalian University of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the expression of MAP4K1 in melanoma cell lines and tissues and the effect of MAP4K1 on the migration and invasion of melanoma cell lines and molecular mechanism. Methods Fifty-four patients with melanoma admitted to the Department of Dermatology of Chongqing Traditional Chinese Medicine Hospital from January 2010 to December 2015 were selected. The expressionof MAP4K1 in melanoma was analyzed by online database. The expression of MAP4K1 mRNA and protein in melanoma was detected by qRT-PCR and immunohistochemistry. The MAP4K1 gene knockout was detected by Western blot and RT-PCR. The effecton migration and invasion of A375 cells after MAP4K1 gene knockout was detected by scratch assay and Transwell assay. The regulation of YAP/TAZ by MAP4K1 was detected by dual luciferase reportergene assay. The correlation between MAP4K1 and the expression of downstream target of Hippo-YAP pathway was proved by GEPIA online database. The effect on Hippo-YAP signaling pathway after MAP4K1 gene knockout was verified by Westernblot. Results Compared with the adjacent tissues, the expression of MAP4K1 mRNA and protein in cutaneous malignant melanoma cells and tissues was up-regulaged (P<0.001). The migration and invasionof A375 cells after MAP4K1 gene knockout were significantly inhibited (P<0.001). The transcription activity of Yap/TAZ after MAP4K1 gene knockout and downstream target molecules were down-regulated. The Hippo-YAP signaling transduction pathway was inhibited by MAP4K1 gene knockout. Con原 clusion The expression of MAP4K1 in melanoma is up-regulated. The MAP4K1 can be served as potential oncogene to promote the development of melanoma by regulating Hippo-YAP pathway. [ABSTRACT FROM AUTHOR]

Copyright of Practical Oncology Journal is the property of Journal of Practical Oncology Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In order to study the bearing mechanical properties of 2D woven ceramic matrix composites with different depths and angles of countersunk head bolted joints, tensile tests were carried out under an uniform tightening torque, the tensile load-displacement curves of the joints with different sink depth and angle were obtained. Referring to the data processing method in ASTM D5961 standard, the hearing strength and the ultimate load were analyzed. The relationship between the bearing strength and countersunk head angle and depth was obtained. The whole field strain distribution near the joint hole of the composite plate was observed by DIG 3D Digital Image Correlation) system. The continuous strain accumulation process and the typical strain nephogram for various failure modes were obtained by DIC. The failure modes were also observed by three-dimensional microscope. The research can provide a reference for the engineering design of ceramic matrix composites countersunk bolted joints. [ABSTRACT FROM AUTHOR]

Copyright of Acta Materiae Compositae Sinica is the property of Acta Materiea Compositae Sinica Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Harbin Institute of Technology. Social Sciences Edition / Haerbin Gongye Daxue Xuebao. Shehui Kexue Ban is the property of Harbin Institute of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Nursing of Integrated Traditional Chinese & Western Medicine is the property of Journal of Clinical Nursing in Practice (Editorial Board, Shanghai Jiao Tong University Press) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)