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Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

[Objective] Macrolactins (MLNs) are a class of 24-membered macrolides formed by cyclization of polyketones, which have a variety of biological activities such as antibacterial, antifungal, antiviral, and anti-tumor. However, due to the low yield of macrolides in traditional fermentation, its development and application are limited. Therefore, this article aims to increase the yield of MLNs synthesized by Bacillus siamensis K53, in order to provide scientific basis for the mass production of MLNs. [Method] Based on previous research, single factor experiments was used to study the effects of carbon, nitrogen and amino acids on the MLNs yield of strain K53 and to determine the key nutritional factors. Response surface analysis was used to optimize the key nutritional factors for obtaining the optimal parameters in fermenting MLNs. [Result] In the single factor experiment, glucose was the optimal carbon source in the fermentation medium of strain K53. The yeast extract and (NH4)2SO4 were the optimal organic and inorganic nitrogen sources, respectively. The addition of histidine and valine increased the yield of MLNs. In response surface optimization, the optimal fermentation medium for strain K53 was 22.0 g/L glucose, 3.0 g/L (NH4)2SO4, 1.0 g/L yeast extract, 6.1 g/L valine, and 5.9 g/L histidine. The valine and histidine were precursors for the synthesis of MLNs. The MLNs yield in this medium reached 5.37×10² μg/mL, which was very close to the maximum value (5.46×10² μg/mL) predicted by theory, and 4.10 times higher than that in the non-optimized medium (1.31×10² μg/mL). [Conclusion] The optimized medium formula by response surface methodology is conducive to the MLNs yield of the strain K53, and the results provide theoretical bases for the consequent development and application of the strain K53. [ABSTRACT FROM AUTHOR]

Copyright of Food Research & Development is the property of Food Research & Development Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of China Brewing is the property of China Brewing Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective:To explore the therapeutic effect of Xiangpi Shengji Ointment on the wounds of rats after anal fistularesection and its correlation with PI3K/Akt/mTOR pathway. Methods: SD male rats were randomly divided into four groups: shamoperation group, model group, Xiangpi Shengji ointment group, and MEBO group, with 10 rats in each group. All four groups were usedto create an anal fistula model using No. 0 steel wire. After successful modeling, except for the sham operation group, anal fistularesection was performed under anesthesia, and the wound was kept open to form an infectious anal fistula postoperative wound that was"open, oozing blood, oozing fluid, and purulent secretion." The sham operation group retained the fistula without changing the dressing.The model group was rinsed with physiological saline and disinfected with complex iodine without changing the dressing. The treatmentgroup was given corresponding drugs to change the dressing for the wound, respectively, A total of 10 days. After 10 days, the woundhealing rate and granulation tissue coverage rate of rats in the model group, the Xiangpi Shengji ointment group, and the MEBO group after anal fistula surgery were compared using conventional area detection methods; HE staining was used to observe the pathologicalchanges of perianal tissues of rats in each group; The expression levels of bFGF, EGF, and VEGF in serum of rats in each group weredetected by ELISA, and the protein expression levels of PI3K, Akt, mTOR, p70 S6K, p-PI3K (S473), p-AKT (S473), p-mTOR (Ser2448),and p-p70 S6K in perianal granulation tissue of rats in each group were compared by WB detection. Results: Compared with the modelgroup, the wound healing rate and granulation tissue filling rate of the Xiangpi Shengji Ointment group and the MEBO groupsignificantly increased on the 3rd, 7th, and 10th day of treatment (P<0.01); Pathological sections showed that in the sham operationgroup, there was less inflammatory cell infiltration, while in the other groups, there were different degrees of inflammatory cellinfiltration, and different degrees of inflammatory repair type granulation tissue proliferation, collagen fiber regeneration, and vasculardilation were visible in the wound area. Pathological sections of the model group showed a large amount of inflammatory cell infiltration, significant vascular dilation, and significant vascular bleeding. Pathological sections of the Xiangpi Shengji Paste group showed fewerinflammatory cells, Fibroblasts are mature and evenly distributed, and collagen fibers are abundant and neatly arranged in the dermis. Angiogenesis is visible, without significant vasodilation and bleeding. Pathological sections in the MEBO group show a small amount ofinflammatory cell infiltration and vasodilation, and fibroblasts are visible in the dermis. The results of ELISA showed that the levels ofb FGF, EGF, and VEGF in the serum of the model group were significantly lower than those of the sham operation group (P<0.01);Compared with the model group, the content of bFGF, EGF, and VEGF in the serum of the Xiangpi Shengji Ointment group wassignificantly increased (P<0.01), while the content of EGF, VEGF in the serum of the rats in the MEBO group was significantly increased(P<0.01). The WB test results showed that compared with the sham operation group, the expression of p-PI3K, p-Akt, p-mTOR, andp-p70 S6K protein in the perianal tissue of the model group was significantly decreased (P<0.01); Compared with the model group, theexpression of p-PI3K, p-Akt, p-mTOR, and p-p70 S6K protein in perianal tissue in the Xiangpi Shengji ointment group was significantlyincreased (P<0.05). Conclusion: Xiangpi Shengji Ointment can effectively promote wound repair after anal fistula surgery, alleviateinflammatory reactions on the wound after anal fistula surgery, and promote the growth of wound granulation. Its healing mechanism maybe related to the expression of PI3K/Akt/mTOR signal pathway related proteins, which can accelerate the process of wound repair byupregulating PI3K/Akt/mTOR signal pathway related proteins, activating PI3K/Akt/mTOR signal pathway. [ABSTRACT FROM AUTHOR]

Copyright of Modern Food Science & Technology is the property of Editorial Office of Modern Food Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Southwest China Journal of Agricultural Sciences is the property of Editorial Department of Southwest China Journal of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Guihaia is the property of Guihaia Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Southern Agriculture is the property of Journal of Southern Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)