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目的探讨整合素β4（ITGB4）对脑血管生长发育的调控作用。方法通过逆转录PCR（RT-PCR）检测靶向itgb4 mRNA序列的吗啉环（MO）在斑马鱼胚胎中的敲低效果。利用体视显微镜和激光共聚焦显微镜（laser scanning confocal microscope， LSCM）观察抑制itgb4表达后，斑马鱼胚胎脑部血管的发育变化。通过在斑马鱼胚胎中注射4，6-联脒-2-苯基吲哚（DAPI）染料检测血脑屏障（BBB）发育的完整性。使用小干扰RNA（small interfering RNA， siRNA）在永生化人脑微血管内皮细胞（hCMEC/D3）中敲低ITGB4，用蛋白免疫印迹（WB）检测其敲低效果。通过活细胞成像分析仪（IncuCyte®）、基质胶（Matrigel® matrix）成管实验和transwell迁移实验分别检测ITGB4在hCMEC/D3中调控细胞增殖、成管和迁移的作用。应用实时荧光定量PCR（qRT-PCR）检测敲低itgb4后Wnt/β-catenin信号通路下游靶基因表达变化。结果在斑马鱼胚胎中敲降itgb4引起脑水肿、脑部出血、血脑屏障形成障碍以及后脑血管数量减少等相关表型。细胞实验结果显示，在hCMEC/D3细胞中抑制ITGB4的表达导致细胞增殖减缓，管腔形成减少及迁移能力减弱。此外，qRT-PCR结果表明，在斑马鱼胚胎中敲低itgb4抑制Wnt/β-catenin通路下游靶基因的表达。最后，在hCMEC/D3细胞中敲低ITGB4后，通过添加Wnt/β-catenin通路激活剂（BML-284）能明显挽回迁移减少的表型。结论ITGB4通过调节脑血管内皮细胞的增殖、迁移和成管等功能促进脑血管发育并参与调控血脑屏障的形成。

Objective To explore the mechanism of Nuanxinkang (NXK, composed of Ginseng Radix et Rhizoma Rubra and Ilex Pubescen) on alleviating myocardial fibrosis in mice with myocardial infarction by orchestrating myocardial cell metabolic remodeling based on macrophage polarization. Methods (1) C57BL/6J male mice were randomly divided into three groups：sham operation group, model group and NXK administration group (1.65 g·kg-1), with 10 rats in each group. The myocardial infarction model of mice was induced by ligating coronary artery. The drug administration group was given NXK once a day for four weeks. Masson staining was used to detect myocardial fibrosis, and quantitative PCR was used to detect the mRNA expression levels of LDHA, HK2, CD36, CPT-1A, PPAR-γ, PGC-1α, Ndufa8, SDHd, Cyc1, Cox4i2, CD86, iNOS and TNF-α in cardiac tissues. The content of inflammatory factor IL-1β in serum was detected by ELISA. (2) Lipopolysaccharide (LPS) was used to induce the polarization of RAW264.7 macrophages into pro-inflammatory subtype. The supernatant fluid of pro-inflammatory macrophages was prepared and subsequently acted on HL-1 cardiomyocytes. RAW264.7 macrophages and HL-1 cardiomyocytes were divided into control serum group (culture medium with 5% blank serum and 5% fetal bovine serum), NXK-contained serum group (culture medium with 5% NXK-contained serum and 5% fetal bovine serum), LPS treatment group (culture medium with 5% blank serum, 5% fetal bovine serum and 200 µg·mL-1 LPS) and NXK-contained serum+ LPS treatment group (culture medium with 5% NXK-contained serum, 5% fetal bovine serum and 200 µg·mL-1 LPS). After 24-h intervention in each group, the lipid in the cells was labeled by Oil Red O staining, and the reactive oxygen species (ROS) level of the cells was detected by DCFH-DA fluorescent probe. Results (1) Compared with the model group, collagen deposition area in cardiac tissue of mice was remarkably reduced (P＜0.01) and local ventricular wall damage in the infarcted area was alleviated in NXK group. Also, the mRNA expressions of LDHA, HK2, CD36, CPT-1A, PPAR-γ, Ndufa8, SDHd, Cyc1, Cox4i2, CD86, iNOS and TNF-α were significantly decreased (P＜0.05, P＜0.01, P＜0.001). The mRNA expression of PGC-1α was significantly increased (P＜ 0.05). Serum IL-1β level was obviously decreased (P＜0.001). (2) Compared with the LPS group, the deposition of lipid droplets in cytoplasm of HL-1 cardiomyocytes in NXK-contained serum + LPS treatment group was remarkably reduced (P＜0.05) and ROS level decreased significantly (P＜0.001). Conclusion NXK can alleviate myocardial fibrosis and ventricular remodeling after myocardial infarction in mice, and its mechanism may be related to inhibiting the polarization of pro-inflammatory macrophages, alleviating inflammation level, reducing the production of ROS, reducing the level of glycolysis in cardiac tissue, and improving oxidative phosphorylation and fatty acid metabolism. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Anhui Agricultural Sciences is the property of Journal of Anhui Agricultural Sciences Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Science & Technology (1008-0864) is the property of Journal of Agricultural Science & Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To explore the application effect of hypertension suture technology in clinical practice and the analysis and treatment of the causes of related complications. Methods From January 2022 to March 2023, a total of 162 outpatients and inpatients were treated with hypertension suture technology in the Medical Aesthetic Center of the People's Hospital of the Autonomous Region, including 95 cases in the facial surgery area, 8 cases in the chest wall surgery area, 26 cases in the abdominal surgery area, 23 cases in the upper limb surgery area, 4 cases in the lower limb surgery area, 6 cases in the back surgery area, The length of the operating area is 1.2 to 13.6 cm, and the width of the surgical area is 0.2 to 5.3 cm, the follow-up was carried out in 3, 6 and 12 months to evaluate the postoperative effects and complications. Results All 162 patients had one-stage healing, 3 patients had absorbable threads discharged from the surgical area, 2 patients had fat liquefaction, 2 patients had blisters, and 1 patient had blood blisters, all of which were healed in stage one after treatment. Conclusion Overreductive suture surgery is definitive but should be used appropriately to reduce associated complications. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Shanxi Agricultural Sciences is the property of Shanxi Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Asian Journals of Ecotoxicology is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Objective To analyze the acupoint compatibility rules of acupuncture and moxibustion in the treatment of male infertility by using complex network technology, and to provide a basis for clinical acupuncture and moxibustion treatment and research on acupoint selection and prescription for male infertility. Methods The clinical literatures on acupuncture and moxibustion in the treatment of male infertility in the past 20 years were retrieved from the database of CNKI, Wanfang Data Knowledge Service Platform, VIP. com and PubMed, and relevant literatures were screened according to the inclusion and exclusion criteria, and a prescription database of acupuncture and moxibustion in the treatment of male infertility was established. The Apriori algorithm in SPSS Modelerl8. 0 was used for correlation analysis, the core acupoints nodes and compatibility relationship were analyzed, and the complex network co-occurrence map was drawn by Gephi0. 9. 2 software. Results A total of 91 literatures that met the standards were finally obtained, and 134 acupuncture prescriptions were extracted, involving 70 acupoints, and the total frequency of acupoints was 666 times. Among them, Guanyuan (RN4) was most frequently used (75 times, 11. 26%), followed by Shenshu (BI23), Sanyinjiao (SP6), Zusanli (ST36), Qihai (RN6), and Mingmen (GV4) points. The main acupoints returned to the Ren channel and the bladder channel of Foot Taiyang. Jiaohui acupoints were most frequently used for specific acupoints, followed by Wushu acupoints and Mu acupoints. Through complex network topology analysis, it was found that Guanyuan (RN4), Zusanli (ST36), Qihai (RN6), Shenshu (BI23), Sanyinjiao (SP6), Taichong (LR3) and other 23 acupoints were the core node points for acupuncture treatment of male infertility. Conclusion The acupoints selected in the acupuncture treatment for male infertility are mainly distributed in the lower abdomen, back and lower extremities, and Jiaohui acupoints, Wushu acupoints, and Mu acupoints are mostly selected for specific acupoints. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Chinese Institute of Food Science & Technology / Zhongguo Shipin Xuebao is the property of Journal of Chinese Institute of Food Science & Technology Periodical Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Chemical Engineering of Oil & Gas / Shi You Yu Tian Ran Qi Hua Gong is the property of PetroChina Southwest Oil & Gas Field Company and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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Copyright of Journal of Xinyang Normal University Natural Science Edition is the property of Journal of Xinyang Normal University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

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