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Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

为快速、准确监测水产养殖环境水体中孔雀石绿含量，建立了一种超高效液相色谱-串联质谱法(UPLC-MS/MS)测定水产养殖水体中孔雀石绿及隐色孔雀石绿的方法.水样经甲酸酸化预处理后，采用多壁碳纳米管固相萃取柱富集和净化目标物，超高效液相色谱分离，三重四级杆质谱检测，氘代同位素内标定量.在优化条件下，孔雀石绿和隐色孔雀石绿在0. 02~10. 0 µg • L-1浓度范围内均满足线性关系，相关系数R2>0. 998，方法检出限、定量限分别为0. 000 3和0. 001 µg • L-1.在0. 005~0. 100 µg • L-1添加水平下，淡水中孔雀石绿加标回收率为98. 7%~107. 8%,隐色孔雀石绿加标回收率为95. 3%~112. 6%，海水中孔雀石绿加标回收率为89. 5%~92. 7%，隐色孔雀石绿加标回收率为94.2%~101.4%，测定相对标准偏差均在7.4%以内.结果表明，所建立的超高效液相色谱-质谱法简单快速、前处理成本低、灵敏度高、重现性好、回收率高，适合于养殖水体中孔雀石绿的测定.