【Objective】 The effect of different concentrations of brassinolide (BR) on growth and physiological characteristics of Matthiola incana L.seedlings under salt stress were explored, which provided theoretical basis for resistance cultivation to salt of M.incana L. 【Method】 Under the pot cultivation condition, M.incana L.seedlings were treated with 0.5% complex salt (NaCl mixed with Na2CO3 with same concentration), then were sprayed with 0, 0.02, 0.04, 0.06, 0.08 and 0.10 mg/L BR, respectively. The variation of plant height, stem diameter, root length, fresh weight, the content of Chl, SS, SP and Pro, the activity of CAT, POD, SOD, REC, MDA content and physio-chemical indexes were analyzed, as each index was comprehensively evaluated according to subordinate function. 【Result】 With the condition of 0.5% salt stress, the plant height, stem diameter, main root length and fresh weight of M.incana L.treated with different BR concentration increased by 0.72-11.83 mm, 0.10-0.32 mm, 3.89-19.88 mm and 0.19-0.99 g/plant, respectively; Chl, SS, SP and Pro content all increased as Chl and Pro content treated with 0.08 mg/L BR were the highest, and SS and SP content treated with 0.06 mg/L BR were the highest; The activity of POD, CAT and SOD all increased, which were all the highest when treated with 0.08 mg/L BR; The content of REC and MDA both decreased, among which REC content treated with 0.08 mg/L BR was the lowest (decreased by 29.22%), and MDA content treated with 0.06 mg/L BR was the lowest (decreased by 16.73%). The subordinate function analysis indicated that 0.06>0.08>CK>0.04>0.10>0.02>0 mg/L, among which the subordinated function value of 0.04 mg/L BR was the highest (0.7038).【Conclusion】 Under the stress of 0.5% salt concentration, spraying BR with different concentrations can increase the plant height, stem diameter, root length, fresh weight and the content of Chl, SS, SP and Pro content, improve the activity of POD, SOD and CAT, all delay the increase of REC and MDA content, so as to reduce the inhibition of plant growth by salt stress, reduce the damage of salt stress to plants, and improve the salt tolerance of plant. The tolerant effect of M.incana L.to salt stress is the best with spraying 0.06 mg/L BR. [ABSTRACT FROM AUTHOR]