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Floats are the primary buoyancy component of plastic fish cages in offshore aquaculture. They can increase fish cage stress and deformation under the action of sea currents, and the rise of the float's surface liquid level can cause waves on the fish cage walkway. Although plastic fish cages are a recent development in aquaculture, little research has been conducted on the arrangement of floats. Therefore, studying the resistance performance of floats is significant for safe offshore aquaculture production. This article first studies the resistance of single and double floats through on-site measurement methods. Then, based on viscous flow theory, numerical simulations are carried out to simulate the resistance and waveforms of single and double floats under different flow velocities. The simulation results are compared with the on-site measurement results to verify the accuracy of the numerical simulation. Using the same numerical simulation method as above, numerical simulations are carried out for square and diamond two typical float arrangement forms, to analyze liquid level and resistance under different flow velocities. The results show that the numerical model agrees with the on-site measurement results, with an error of 9. 5%. Three aspects are used to analyze the advantages and disadvantages of square and diamond arrangements. (1) Liquid level rise: When the flow velocity is the same, the total liquid level rise of the diamond-shaped floats arrangement is less than that of the square arrangement. (2) Flow field distribution in the middle of two floats: The flow field between the two floats of square arrangement is more chaotic than that of diamond arrangement. (3) Average resistance: When the flow velocity is greater than 1. 5 m/ s, the resistance of diamond arrangement is 29% less than that of square arrangement. The results of the on-site measurement of the small boat towing test are compared with the results of the numerical simulation output. The resistance and waveforms agree with the test results, proving that the numerical simulation method used in this article is accurate and reliable and can provide technical support for subsequent numerical studies of floats. Analyzing the liquid level situation and liquid level cloud images of the two arrangements shows that the diamond arrangement has more stable liquid level changes and better air gap performance than the square arrangement, so it is more suitable for complex offshore environments. The diamond arrangement can provide a better working environment for offshore aquaculture. By sorting out and analyzing the results of numerical simulation, it is found that the average resistance of square arrangement and diamond arrangement is less than that of individual floats. Further comparing average resistance shows that diamond arrangement has less average resistance than square arrangement, so it is more suitable for complex offshore conditions. Floats are the primary buoyancy components of plastic fishing rafts used in offshore aquaculture. Under the influence of sea currents, the resistance generated by floats can result in increased force and deformation of the fishing raft. Additionally, the elevation of the liquid level on the float's surface can induce waves on the raft platform. Therefore, studying the resistance performance of floats is crucial for ensuring the safety of offshore aquaculture production. The resistance performance of both single and double floats was investigated through practical measurements at sea. Numerical simulations based on viscous flow theory were employed to analyze the resistance and waveform of single and double floats at various flow rates. The accuracy of the numerical simulations was validated by comparing the results with actual sea measurements. Furthermore, numerical simulations were conducted to assess the placement of two typical floats in square and diamond configurations. Liquid surface heights and resistance at different flow rates were analyzed. The findings indicate that numerical simulations align well with actual sea measurements, with an error rate of less than 9. 5%. The diamond-shaped float configuration corresponds to an elevation in the liquid surface, creating a more stable flow field between the two floats. This results in a smaller average resistance, with values below 29% in flow velocities exceeding 1. 5 m/ s. Consequently, it is recommended that this placement configuration be adopted for production operations. [ABSTRACT FROM AUTHOR]

Objective To investigate the effects of anlotinib on proliferation and apoptosis of human glioma cells (T98G cells) through nuclear transcription factor-κB (NF-κB) signaling pathway. Methods T98G cells were cultured in vitro and randomly divided into the control group, 5 µmol/L anlotinib group, 10 µmol/L anlotinib group, 20 µmol/L anlotinib group, positive drug group, and inhibitor group. Cells in the control group were not intervened, cells in the 5, 10, and 20 µmol/L anlotinib group were treated with 5, 10 and 20 µmol/L anlotinib, respectively, and cells in the positive drug group were treated with 50 mg/L 5-fluorouracil; in the inhibitor group, 10 µmol/L anlotinib +5 µmol/L NF-κB pathway inhibitor BAY 11-7082 were added for intervention. Cell viability was measured by CCK-8, cell proliferation rate was measured by 5-acetylidene-2 'deoxyuracil riboside method, and apoptosis rate was measured by Hoechst33258 staining. The expression levels of cell cycle proteins D1 (CyclinD1), Caspase-3, NF-κB p65 and phosphorylated (p-) NF-κB p65 were detected by Western blotting. Results Compared with the control group, there was no significant difference in cell viability in the 5 µmol/L anlotinib group (P>0. 05), while the cell viability in the 10, 20 µmol/L anlotinib groups and positive drug group was lower (all P<0. 05). Compared with the control group, the cell proliferation rates, and the expression levels of CyclinD1 and p-NF-κB p65 protein were lower, while the apoptosis rates and the expression levels of Caspase-3 protein were higher in each anlotinib group and positive drug group (all P<0. 05). Compared with the positive drug group, the cell proliferation rate, the expression levels of CyclinD1 and P-NF-κB p65 protein in the 10 µmol/L anrotinib group were higher (all P<0. 05), while the apoptosis rate and the expression of Caspase-3 protein were lower (both P< 0. 05). There was no significant difference between 20 µmol/L anlotinib group and positive drug group (P>0. 05). Compared with the 10 µmol/L anrotinib group, the changes of all indexes in the inhibitor group were more significant (all P< 0. 05). Conclusion Anlotinib may inhibit proliferation and promote apoptosis of human glioma cells by inhibiting NFκB pathway signaling. [ABSTRACT FROM AUTHOR]

In order to solve the problem that aluminum coating on bolt surface may be uneven in thickness when aluminum paint was used to coat the bolts, the influence of spraying angle on the uniformity of bolt surface coating is researched in this thesis. Nozzles and bolts of the spraying system were modeled and the computational fluid dynamics simulation software Fluent was used to establish the physical model of the gas-liquid two-phase flow of the atomization flow field. DPM model was used to analyze the interaction between the gas phase and the liquid phase in the flow field. Besides, the spray flow fields at different angles were obtained. The thickness of liquid film deposition on the bolt surface was also predicted and the spraying test system was set up. Results of this research were verified with the simulation results. The simulation and experimental results all show that the bolt surface coating is the most uniform when the spraying angle is 30°. [ABSTRACT FROM AUTHOR]

The Haihe River Basin is one of the most important grain production bases in China, such as winter wheat and summer corn. Natural precipitation cannot fully meet the water demand of crops in this region. Supplementary irrigation has been widely used to meet the growth needs of crops. The annual irrigation water volume has exceeded 20 billion m3, in order to ensure national food security. Large-scale irrigation has posed a significant impact on the climate, thus altering the local ecological environment, crop cultivation and growth in the irrigation area. Therefore, there is a high demand to explore the impact of irrigation on the climate, particularly for a comprehensive understanding of regional climate change, water resource management, and ensuring food security. In this study, a new irrigation scheme was developed in the land surface module of the Weather Research and Forecasting (WRF) model, in order to simulate the climatic effects of large-scale irrigation in the Haihe River Basin. Actual statistical irrigation water volume data was used with the proportion of grid unit cultivated land area to total cultivated land area as the weight to spatially distribute the irrigation water volume. Then, the irrigation water volume was distributed to the month, according to the irrigation area and irrigation system of the main crops. The results indicated that the irrigation caused an increase in the soil moisture in farmland, leading to an increase in the evaporation, and latent heat flux, whereas, there was a decrease in the sensible heat flux. Irrigation led to an average increase of 10.76W/m2 in the latent heat flux, and a decrease of 7.19W/m2 in the sensible heat flux from April to June. The increase in the latent heat flux was consistent with the decrease in the sensible heat flux in both time and space. The increase in the latent heat flux was greater than the decrease in the sensible heat flux. The larger the irrigation area and month were, the greater the increase in latent heat flux and the decrease in sensible heat flux. Irrigation had a significant local cooling effect. There was a 0.30 ℃ decrease in temperature from April to June, with the largest decrease in May, reaching 0.45 ℃. The decrease in temperature in space was positively correlated with the amount of irrigation water, and the cooling from April to June in plain areas mostly exceeded 0.4 ℃. In addition, the cooling effect of irrigation was more limited to the local area, indicating a relatively small impact on the surrounding areas. There was a certain degree of rain increasing. An average increase of 6.07mm was found in the annual precipitation, which was mainly concentrated from April to June, with an increase of 4.83mm. There was an increase in the precipitation in the Ziya and Daqing River Basin from April to June, with the largest increase in the precipitation in the Daqing River Mountain area, ranging from 15 to 30mm. The dominant impact on convective precipitation, with the relatively small changes in the non-convective precipitation. There was the effect of irrigation on the regional climate, meteorological factors, and supported water security in the basin on both supply and demand sides. Regional precipitation also increased for the available water in the basin. At the same time, the irrigation increased the water vapor content near the surface, whereas, there was a decrease in the temperature, and the reference crop evapotranspiration in the watershed by 38.14mm/a, resulting in a decrease in crop water demand per unit area. There was the increasing precipitation while the less crop water demand after irrigation. Therefore, large-scale irrigation can be expected to alleviate the contradiction between water supply and demand in the Haihe River Basin. [ABSTRACT FROM AUTHOR]

Ship docking plays an important role in the shipbuilding industry as a key part of maintaining and repairing ship structures. However, at present, the surface grinding process during ship docking relies on traditional manual work, which has problems such as low efficiency, long working hours and high danger. For this reason, a new cable-driven grinding mechanism was proposed, which used four cable to drive the grinding device to achieve three degrees of freedom of movement. Firstly, the dynamics model of the system was established by Lagrangian method. Then a Fuzzy-PID( proportional integral derivative) control strategy with cable tension optimization term was proposed on the basis of the dynamics model, which could achieve accurate trajectory tracking and ensured the cable was in tension. Finally, the effectiveness of the proposed control strategy was verified by numerical simulation. The results show that the control accuracy of the proposed control strategy is improved by 25% compared with the PID control commonly used in cable-driven parallel robots, which has high control accuracy and stability. The cable-driven grinding mechanism and its control strategy proposed in this paper can provide some theoretical support for applications such as surface treatment and precision manufacturing of large structural parts. [ABSTRACT FROM AUTHOR]

[Objectives]The objective of this experiment was to study the microbial composition, testosterone and endotoxin content of feces, from boars with different semen quality, and analyze the correlation between semen quality and fecal microbiota, testosterone and endotoxin. It will provide a basis for improving semen quality by regulating gut microbiota in pig production. [Methods]According to semen quality parameters of all boars in the three months before the experiment, 33 York boars of similar age(479.33±2.85)and body condition were selected and divided into low semen quality group(LSQ, n=12)and high semen quality group(HSQ, n=21). Fresh feces of experimental boars were collected to detect the content of testosterone and endotoxin, and the microbial composition of feces was analyzed by 16S ribosomal RNA gene sequencing technique. [Results]The fecal testosterone level of boars in the HSQ group was significantly higher than that in the LSQ group(P<0.01), and there was no significant difference in endotoxin levels. There were 3 differential bacteria on family level and 5 differential bacteria on genera level in the boar fecal microbiota of the HSQ group and LSQ group. At the family level, Eubacterium_coprostanoligenes_group was significantly enriched in the LSQ group(P<0.05), Atopobiaceae and Akkermansiaceae were significantly enriched in the HSQ group(P<0.05); at the genus level, g_norank_f_Eubacterium_coprostanoligenes_group and Faecalibaculum were significantly enriched in the LSQ group(P<0.05)and g_norank_f_Atopobiaceae, Akkermansia and Clostridium_sensu_stricto_6 were significantly enriched in the HSQ group(P<0.05). There was a significant negative correlation between Eubacterium_coprostanoligenes_group and sperm motility and sperm density(P<0.01), and there was a negative correlation between Eubacterium_coprostanoligenes_group and fecal testosterone levels(P=0.11); there was a significant positive correlation between Atopobiaceae and sperm motility, sperm density and fecal testosterone levels(P<0.05); Akkermansiaceae significantly positively correlated with sperm motility(P<0.01)and sperm density(P<0.05), and positively correlated with fecal testosterone levels(P=0.09). [Conclusions]The results showed that there were differences in the fecal testosterone level and microbial composition of boars with different semen quality, and there were different degrees of correlations between boar fecal microbial structure and semen quality, fecal testosterone levels. The results provide a new theoretical support for improving boar semen quality by regulating gut microbiota. [ABSTRACT FROM AUTHOR]

The increase in water quality by large reservoirs impoundment will have an impact on the earth internal structure. The change of earth internal structure will change the gravity. The huge water loading caused by the river reservoir after the impoundment of the Three Gorges Reservoir area is bound to respond more obviously to gravity change. In order to study the degree of the gravity change, the formula of the gravity change of the cuboid is deduced. Based on the formula, the influence of the water load change of the Three Gorges Reservoir on the gravity of the ground station under different water storage heights are simulated. At the same time, with the influence of soil water change, the remote gravity change around the reservoir can be interpolated as the influence of other factors. The results show that the cuboid gravity change formula has good calculation results in the simulation of the gravity change caused by the impoundment process. Among many influencing factors, the impoundment is the main one. From the influence of soil water change and the interpolation of remote gravity change around the reservoir, the calculation accuracy is improved much. The average absolute error near the stations in three periods from 7, 10. 4 and 6. 5 μGal raised to 4. 3, 8. 1 and 6. 1 μGal. At the same time, the cuboid model provides reference for later inversion work. [ABSTRACT FROM AUTHOR]

In this paper, transparent intumescent flame retardant coatings were prepared with melamine-formaldehyde resin (MF) as the film-forming resin, phosphoric acid-pentaerythritol (H3PO4 -PER) as the intumescent flame retardant system, and graphene oxide (GO) as the flame retardant co-agent to improve the flame retardant and smoke suppression performance of wood-plastic composites (WPC). Results showed that the coatings had highly transparency, good physical properties, excellent flame retardancy and smoke suppression properties. The addition of GO did not change the physical properties of the coatings, but significantly improved the thermal stability, flame retardancy and smoke suppression properties of the WPC composites. Thermogravimetric analysis (TG) tests showed that the charcoal residue of the coating increased by 11. 0% when 0. 2% GO was added. The simulated large plate burning method showed that the flame resistance time of the coating increased by 75. 0% compared with the control group when 0. 2% GO was added. Cone calorimeter (CONE) combustion test showed that the addition of GO effectively suppressed the heat release and smoke release of the coating. The total heat release and total smoke release of the coating decreased by 35. 6% and 43. 5%, respectively. The flake structure of graphene oxide became cross-linked points after the coating was expanded by heat, which improved the overall mechanical strength and stability of the carbon layer, and then achieved the effect of flame and smoke suppression. [ABSTRACT FROM AUTHOR]

[Objective] The present paper aimed to provide a basis for the recycling of nutrient resources of organic solid waste in urban and rural areas of China and the rational application of compost.[Method] The organic carbon (C) and phosphorus (P) characteristics of four organic waste composts, i.e., food waste (FW), garden waste (GW), chicken manure (ChM) and cattle manure composts (CaM), were systematically analyzed by Hedley P sequential fractionation method, infrared spectroscopy and thermogravimetric analysis. [Result] The obvious differences in the content and fraction of P and the content and composition of organic C among four composts were observed. The total and labile P content of animal manure composts (ChM and CaM) were significantly higher than those of plant-derived organic waste compost (FW and GW), in which the highest content of water extracted P (H2O-P) and NaHCO3 extracted P (NaHCO3-P) was observed in ChM and CaM composts, respectively. FW compost had high total organic C (TOC), but low humification index (i.e., humic C/fulvic C = 0.09) . Meanwhile, FW compost had the highest content of labile organic C (LOC), i.e., dissolved organic C (DOC), light molecular organic acids (LMOA) and amino acids (AA), the lower degree of aromatization with more carboxylic acids and polysaccharide structures, and the lowest thermal stability. GW compost had the highest content of readily oxidizable organic C (ROC) and humic substances C (HSC), and a high aromatized structure and thermal stability. Animal manure compost had the lowest HSC content and the medium DOC content. ChM compost had the highest free AA content and rich LMOA species, while the CaM compost contained a certain amount of AA and oxalic acid. Two animal manure composts had a similar aromatization degree and thermal stability, which was between FW and GW. [Conclusion] Manure composts with high P content and labile organic C can be used as a good P source to completely or partially replace chemical fertilizer. FW compost had low P content but high LOC content, which can improve the availability of P fertilizer via promoting P by C. GW compost with low P content and high stable organic C, in the long run, can improve soil fertility, which in turn can enhance soil P availability. Thus, rational application of composts according to their C and P characteristics or combined application of different composts or compost with chemical fertilizers can realize the C and P synergy and the highly efficient recycling of P nutrient resources. [ABSTRACT FROM AUTHOR]

The dewatering mechanism ol deep-cone rake thickener is complex, which has the problems ol nonlinearity, large delay and low system stability, in order to improve the perlormance ol the rake, the hydraulic jet equipment is installed, and the torque relation model ol rake ol thickener is established, and the PID torque and underflow concentration control algorithm based on double luzzy neural network is established. Simulation results show that the parameters ol the algorithm are better than those ol neural network PID and traditional PID, The algorithm has a stable adjustment time ol 1 709. 32 s and an overshoot ol 2. 55 %・ The application ol the algorithm in engineering shows that the concentration control is more stable, the continuous high concentration lilling time was prolonged by 0. 7 hour. [ABSTRACT FROM AUTHOR]

The study was to optimize the composition of Clostridium butyricum fermentation medium. The effects of different fermentation conditions and fermentation medium components on the biomass of Clostridium butyricum were studied by uniform experimental design and response surface methodology, and the optimal fermentation process parameters were explored. The results showed that four highly significant components of glucose, yeast extract powder, dipotassium hydrogen phosphate and calcium chloride were screened from 16 kinds of fermentation media by using uniform experimental design. Further response surface methodology was used to optimize the combination of the four components, and finally the optimized medium compositions were as follows: Glucose 1.15%, starch 0.34%, peptone 2.05%, yeast extract 2.65%, bran 0.23%, corn pulp 0.21%, potassium dihydrogen phosphate 0.64%, potassium dihydrogen phosphate 0.75%, magnesium sulfate 0.006%, manganese sulfate 0.001 5%, calcium chloride 0.018%, sodium chloride 0.52%, ferrous sulfate 0.018%, calcium carbonate 0.42%. The study indicate that the biomass of Clostridium butyricum measured by the two methods was 18.1×108 CFU/mL, which is 2.42 times higher than that of the initial medium. [ABSTRACT FROM AUTHOR]

Ionospheric foF2 is great important for the short wave communication and over the horizon radar. In this study, an empirical ionospheric foF2 model was developed based on the polynomial method and the global ionospheric foF2, observed by COSMIC from 2006 to 2014. Meanwhile, the independent observations between 2015 and 2019 are used to test this empirical model. As a result, the correlation coefficients between this empirical model and the modeling and testing observations are 0.948 and 0.937. Meanwhile, the corresponding average deviations are about 2.38% and 3.08%, and the relative errors are 11.72% and 12.69%, respectively. The temporal and spatial variations of ionospheric foF2 are also comprehensively discussed by using this empirical model. The day-night variation of ionospheric foF2 increases with the increased latitude, and it is greater in the southern hemisphere than the northern hemisphere during the Spring and Autumn months. However, the diurnal variation of ionospheric foF2 in the summer hemisphere is much lower than that in the winter hemisphere. Significantly, the seasonal variation of ionospheric foF2 at night is mainly annual variation, but dominated by the annual and semiannual variations during the daytime. Moreover, the magnitude of seasonal variation is increasing with the increased latitude, with much greater values at nighttime and in the southern hemisphere. Noteworthy, the mid-latitude trough of ionospheric foF2 at nighttime usually occurs in the Spring and Autumn months, and the 4-waves structure in the longitude mainly occurs under the lower solar activity levels. [ABSTRACT FROM AUTHOR]

Background and purpose: Adriamycin also named as doxorubicin, is one of the most widely used cytotoxic chemotherapeutic agents for clinical practice for the treatment of various tumors, and belongs to anthracycline antitumor drugs. Unfortunately, this drug will cause serious side effects, especially dose-dependent cardiotoxicity, which has become a concern in the field of onco-cardiology. At present, there is no universally recognized, unified and robust method to construct Doxorubicininduced cardiomyopathy model. This experiment was designed to explore the optimal dose and frequency of doxorubicin-induced cardiomyopathy in a mouse model. Methods: Forty 8-10-week-old male C57BL/6J mice were randomly divided into 4 groups (control group and 3 model groups). The model group was divided into model 1 (M1) group (15 mg/kg, single dose) and M2 group (5 mg/ kg, once a day for 3 days continuously) and M3 group (7.5 mg/kg, twice on alternate days). General vital signs (body weight change and survival rate), echocardiography, body surface electrocardiogram (ECG), N-terminal pro-B-type natriuretic peptide (NT-proBNP), cardiac troponin I (cTnI) and myocardial tissue morphological changes were evaluated comprehensively. Results: Compared with the control group, the body weight of mice in M1, M2 and M3 groups decreased significantly (P＜0.001); M3 group had higher survival rate than M1 and M2 group (80% vs 40% and 50%, P＜0.05). Compared with the control group, M1 group and M2 group, the body surface ECG showed that the PR interval of M3 group [(0.064 2±0.003 8)s vs (0.042 3±0.000 9)s, (0.052  7±0.007  9) s and (0.062  0±0.001  2)s, P＜0.05] and QT interval [(0.047  5±0.000  2)s vs (0.022 0±0.000 9)s, (0.038 6±0.004 4)s and (0.044  4±0.003  0)s, P＜0.05] were significantly prolonged. Cardiac ultrasound showed that M3 ejection fraction decreased significantly (40.40%±2.24% vs 54.72%±1.64%, 46.00%±4.41% and 54.68%±3.38%, P＜0.05). M3 short-axis shortening rate decreased significantly (19.40%±1.20% vs 27.88%±1.05%, 22.57%±2.50% and 27.86%±2.20%, P＜0.05). Cardiac markers showed that compared with CON, the serum NT-proBNP levels of M1, M2 and M3 in adriamycin group were significantly increased [(638.13±12.69) pg/mL vs (1 271.36±11.76) pg/mL, (1 270.85±36.19) pg/mL and (1 225.26±24.19)  pg/ mL, P＜0.05] . Histomorphology showed that the vacuoles in cardiomyocyte of M3 group was significantly increased compared with CON, M1 and M2 group (81/field vs 3/field, 65/field and 34/field, P＜0.05). Conclusion: Intraperitoneal injection of adriamycin in acute heart failure model is simple and reliable. The model in which adriamycin is administered by intraperitoneal injection at a dose of 7.5 mg/ kg, twice on alternate days, with cumulative dose of 15 mg/kg is optimal. [ABSTRACT FROM AUTHOR]

To investigate the effects of Tspan8 gene knockout combined with anlotinib on the proliferation, migration, invasion, and apoptosis of colon cancer SW480 cells. Methods The plasmid was constructed by CRISPR/Cas9 technique, and Tspan8 gene was knocked out in SW480 cells. The knockout effect was detected by Western blot. The IC50 of anlotinib was calculated by MTT assay. The experiment was divided into control group, anlotinib group, Tspan8 knockout group, and combined group. Cell proliferation, migration, invasion, and apoptosis were detected by cell proliferation assay, clonal formation assay, scratch assay, Transwell chamber assay, and flow cytometry. Western blot was used to detect the effect of anlotinib on Tspan8 expression in SW480 cells. Results SW480-KO-Ⅲ cells had the highest knockout efficiency in the Tspan8 knockout group. They were used in subsequent experiments. Different concentrations of anlotinib could inhibit the proliferation of SW480 cells at different times (P<0.01) in a concentration-dependent and time-dependent manner (P<0.01). According to IC50, 14 μmol/L was selected as the subsequent experimental concentration. Compared with those in the control group, the cell proliferation, migration, and invasion abilities in the anlotinib group, Tspan8 knockout group, and combined group were significantly decreased, and the cell apoptosis level was significantly increased (P<0.05). The above changes in the combined group were more significant than those in the anlotinib group or Tspan8 knockout group (P<0.05). Compared with that in the control group, the expression level of Tspan8 in SW480 cells in the anlotinib group was significantly decreased (P<0.01). Conclusion Tspan8 gene knockout combined with anlotinib can synergistically inhibit the proliferation, migration and invasion of SW480 cells. This combination can also promote the apoptosis of these cells. [ABSTRACT FROM AUTHOR]

Objective To investigate the value of double-labeled time-resolved fluorescence immunoassay (TRFIA) for quantitatively detecting serum levels of tumor necrosis factor-α (TNF-α) and high-sensitivity C-reactive protein (hs- CRP). Methods Anti-TNF-α and hs-CRP monoclonal antibodies were coated on the 96-well plate, meanwhile prepared the europium (Eu3+) and samarium (Sm3+)-detection antibody conjugates, and then established the double-antibody sandwich TRFIA method and assembly into a kit, and finally evaluated the detection performance of this kit, such as sensitivity, linear range, spike recovery rate. Results A new method for detecting serum TNF- α and hs-CRP levels by TRFIA was successfully established and assembled into a kit. The linear range of the prepared TRFIA kit for TNF-α was 0-100 ng/L, sensitivity was 0.05 ng/L, the linear range for hs-CRP was 0-100 mg/L, sensitivity was 0.02 mg/L. The spiked recovery rate of TNF-α was between 92.00% and 107.00%, and that of hs-CRP was between 95.00% and 106.82%. There was no obvious cross-reaction with the symptom detection indexes. The TNF-α CV of intra-assay was between 4.57% and 9.24%. The interassay was between 5.13% and 9.27%. The hs-CRP CV of intra-assay was between 5.12% and 7.69%, and the inter-assay was 6.07%-10.00%. Additionally, the kit can be stored stably at 4 ℃ for half a year and at 37 ℃ for 7 days. The detection threshold of TNF-α was 0.44 ng/L, and the detection threshold of hs-CRP was 1.41 mg/L. The test results of the kit were consistent with the clinical situation, and the coincidence rate reached 100%. Conclusion The double-labeled TRFIA method can quantitatively detect TNF-α and hs-CRP levels, which has the advantages of high sensitivity, high specificity, convenience and fast. This TRFIA kit provides a new detection method for the early screening, efficacy evaluation and prognostic assessment of clinical samples of sepsis. [ABSTRACT FROM AUTHOR]

BACKGROUND: Relevant studies have shown that anterior cruciate ligament rupture may be related to the anatomical morphology of the distal femur and proximal tibia. OBJECTIVE: To investigate the correlation between anterior cruciate ligament rupture and the match between the tibial intercondylar eminence and the femoral intercondylar fossa. METHODS: A total of 74 male soldier patients who were admitted at the 904 Hospital of the Joint Logistics Support Force from January 2016 to March 2021 and suffered unilateral knee injuries or discomfort due to non-contact mechanism were selected. According to X-ray and magnetic resonance imaging findings, they were divided into two groups (n=37 per group): an anterior cruciate ligament rupture group and an intact anterior cruciate ligament group. Width index of the intercondylar fossa was detected using coronal magnetic resonance imaging, and the morphology of the intercondylar fossa was observed using axial magnetic resonance imaging. In X-ray, posteroanterior films were used to detect the width, lateral and medial height of the tibial intercondylar eminence. Ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa was used to describe the match between the tibial intercondylar eminence and the femoral intercondylar fossa. RESULTS AND CONCLUSION: There was no significant difference between the two groups in the width of femoral bicondyle, the lateral and medial heights of the tibial intercondylar eminence (P > 0.05). The width of the intercondylar fossa, the width index of the intercondylar fossa, the width of the tibial intercondylar eminence, and the ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa in the anterior cruciate ligament rupture group was lower than that in the intact anterior cruciate ligament group (P < 0.05). The A-shaped intercondylar fossa was more common in the anterior cruciate ligament rupture group, and the U-shaped intercondylar fossa was more common in the intact anterior cruciate ligament group. Univariate Logistic regression analysis showed that the width of the intercondylar fossa, the width index of the intercondylar fossa, the morphology of the intercondylar fossa, the parameters of the tibial intercondylar eminence, and the ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa were all the influencing factors for anterior cruciate ligament rupture (P < 0.05). Multivariate Logistic regression analysis showed that the ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa was an independent risk factor for anterior cruciate ligament rupture (P < 0.05). Receiver operating characteristic curve showed that the ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa had a certain predictive value for anterior cruciate ligament rupture. To conclude, the ratio of the width of the tibial intercondylar eminence to the width of the intercondylar fossa is an independent risk factor for anterior cruciate ligament rupture. It has a certain predictive value for anterior cruciate ligament rupture. The worse the match between the tibial intercondylar eminence and the femoral intercondylar fossa is, the higher the risk of anterior cruciate ligament rupture is. [ABSTRACT FROM AUTHOR]

BACKGROUND: The anatomical parameters of the femoral condyle and tibial plateau affect the biomechanics of the knee joint. The matching of the medial femoral condyle and the medial tibial plateau may affect the magnitude of the annular stress on the medial meniscus, and thus affect the posterior root tear of medial meniscus. OBJECTIVE: To evaluate the effect of the match between the medial femoral condyle and the tibial plateau on the tear of the posterior root of the medial meniscus. METHODS: The data of patients who underwent knee MRI examination at the 904 Hospital of the Joint Service Support Force of Chinese PLA due to knee joint pain from January 2016 to January 2020 were retrospectively analyzed. Finally, 52 patients with medial meniscus posterior root tear were selected as observation group; 40 patients without abnormal bone and soft tissue anatomy were selected as control group. The anatomical parameters of the knee joint were measured on MRI: medial femoral condyle anteroposterior diameter, medial tibial plateau anteroposterior diameter, medial femoral condyle transverse diameter, and the transverse diameter of the medial tibial plateau. The lateral diameter ratio of the medial femoral condyle to the medial tibial plateau and the anteroposterior diameter ratio of the medial femoral condyle to the medial tibial plateau were calculated. At the same time, receiver operating characteristic curve analysis was performed to determine the critical value of risk factors for posterior root tear of the medial meniscus. RESULTS AND CONCLUSION: (1) The medial femoral condyle transverse diameter and medial femoral condyle anteroposterior diameter were greater in the observation group than those in the control group (P < 0.05). The lateral diameter ratio of the medial femoral condyle to the medial tibial plateau and the anteroposterior diameter ratio of the medial femoral condyle to the medial tibial plateau were greater in the observation group than those in the control group (P < 0.05). There were no significant differences in the transverse diameter of the medial tibial plateau and medial tibial plateau anteroposterior diameter between the two groups (P > 0.05). (2) Receiver operating characteristic curves demonstrated that the best cut-off value of the transverse diameter ratio of the medial femoral condyle to the medial tibial plateau was 0.85, the sensitivity was 57.69%, and the specificity was 97.50%; area under the curve was 0.815. The best cut-off value of the anteroposterior diameter ratio of the medial femoral condyle to the medial tibial plateau was 1.29, and the sensitivity was 61.54%; the specificity was 95.00%; area under the curve was 0.710. (3) These findings suggest that the lateral diameter ratio of the medial femoral condyle to the medial tibial plateau greater than 0.85 and the anteroposterior diameter ratio of the medial femoral condyle to the medial tibial plateau greater than 1.29 may be an important risk factor for the occurrence of posterior root tear of the medial meniscus. The method of measuring the lateral diameter ratio and the anteroposterior diameter ratio of the medial condyle to the plateau in coronal and sagittal MRI views of knee joint has a high sensitivity and detection rate for the diagnosis of posterior root tears of the medial meniscus. . [ABSTRACT FROM AUTHOR]

The mutation rate of anaplastic lymphoma kinase ( ALK) in patients with non-small cell lung cancer is 3%, to 7%. Due to its low mutation rate and better long-term survival compared with epidermal growth factor receptor-positive non-small cell lung cancer patients, therefore, it' s called " diamond mutation" . At present, there are three generations of ALK tyrosine kinase inhibitor (TKI) drugs in the world. The first-generation ALK-TKI drug approved in China is crizotinib, and the second-generation drugs are alectinib, ceritinib and ensartinib. Among them, ensartinib is an ALK-TKI domestically developed, and its efficacy is similar to that of alectinib. The main adverse event is transient rash, and compliance to cnsa11inib is better from the perspective of long-term survival of patients. The manifestation of rash caused by cnsat1inib is different from that of other ALK-TKI drugs. In order to facilitate clinical application and provide patients with more treatment options, under the guidance of the Committee of Cancer Rehabilitation and Palliative Care of China Anti-Cancer Association, this article collects and summarizes the common adverse reactions of ensartinib. Based on the clinical practice, a clear adverse classification and specific treatment plan are formulated, in order to provide a corresponding reference for clinicians to make more comprehensive clinical decisions. [ABSTRACT FROM AUTHOR]

To investigate the effects of sodium hypochlorite and chlorhexidine as root canal disinfection rinsing solution on oral flora and root canal infection substances. Seventy-six patients who received root canal treatment were divided into observation group and control group according to the random number table method, in which the control group was treated with sodium hypochlorite and the observation group was treated with sodium hypochlorite and chlorhexidine combined. The detection rate of oral flora, bacterial colony count in the root canal, infectious substances in the root canal, therapeutic effect, long-term effective rate and incidence of adverse reactions in two groups were compared and analyzed. After root canal disinfection and irrigation, the bacterial colony count and root canal infection substances in the observation group were significantly lower than those in the control group, with statistical significance (P<0.05). The incidence of total effective rate and adverse reaction in the observation group was 100.00% and 13.16%, respectively, and the incidence of total effective rate and adverse reaction in the control group was 88.00% and 65.79%, respectively (P<0.05). In addition, the effective rate of observation group was higher than control group at 3 months, 6 months and 1 year later. Sodium hypochlorite and chlorhexidine, as root canal disinfection rinsing solutions, can effectively reduce the number of oral flora and infectious substances in the root canal, improve the treatment efficiency and reduce the incidence of adverse reactions. Therefore, they are worthy of widespread clinical use. [ABSTRACT FROM AUTHOR]

A long-term site-specific experiment of fertilizer recommendation from 2012 to 2020 was conducted to evaluate the comprehensive effects of nutrient expert decision support system on yield, benefit, fertilizer use efficiency, and soil nutrient of spring maize in northeast China. Five treatments included no-fertilizer (CK) as the control, currently traditional farmers’ practices (FP), fertilizer recommended using a nutrient expert system (NE), slow/controlled-release nitrogen fertilizer using the same rate as NE treatment (NER), and conventional fertilizer recommendation using soil testing (ST). The current study also investigated the long-term changes of maize yield, benefit, fertilizer use efficiency, the contents of inorganic N, available P, and available K in the soil, as well as the balance of nutrient input/output during the nine-year period. The results showed that the maximum, minimum, or mean values of fertilizer inputs in NE, NER, and ST treatments were all significantly lower than that of FP treatment (P<0.05), respectively. In different nutrients, NE, NER and ST treatments significantly reduced the amounts of nitrogen (N) and phosphorus (P) fertilizers application (P<0.05), but significantly increased the amount of potassium (K) fertilizer application (P<0.05), compared with that of FP treatment, respectively. Compared with FP treatment, NE, NER, and ST treatments significantly improved the stability of maize yield and net income, where increased by 5.6%-11.0% and 8.3%-14.3%, respectively. The NE treatment achieved the highest value, followed by ST and NER treatment. The recovery efficiency, agronomic efficiency, and partial factor productivity in the NE, NER, and ST treatments were significantly higher than those in FP treatment, where increased by 29.0%-40.1%, 31.3%-44.3%, and 22.0%-31.7%, respectively. Specifically, the highest value was observed in NE treatment, followed by NER and ST treatments. Compared with FP treatment, NE, NER and ST treatments significantly improved the inorganic N content in 0-30 cm soil layer (P<0.05), but significantly reduced inorganic N content in 30-90 cm soil layer (P<0.05) and available P content in 0-30 cm soil layer (P<0.05). But the soil available K content wasn’t significantly different (P>0.05) among different fertilization treatments. The contents of soil inorganic N, available P, and available K in NE treatment were close to the initial testing values before planting. The nutrient input/output balance was obtained during the nine-year period, where the N and P balances were surplus under all fertilization treatments, whereas, the K balance was deficient. But the surplus of N, P, and the deficiency of K in NE treatment attained the lowest values, followed by NER and ST treatments. In conclusion, compared with farmers’ practices and the conventional fertilizer recommendation system, the NE fertilization recommendation system fully met the nutrient requirements of maize, with optimal fertilization rate, time, and ratio. Consequently, the NE fertilization recommendation system has the potential to improve maize yield, benefits, and fertilizer use efficiency, thereby maintaining the soil nutrient stability under the reduction of fertilizer application amount, compared with farmers’ practices. However, the simplified fertilization of slow/controlled-release nitrogen fertilizer in the NE fertilization recommendation system also obtained higher benefits, but reduced labor cost. Therefore, the NE system is an appropriate fertilizer recommendation for maize in northeast China. [ABSTRACT FROM AUTHOR]

The current common types of biological membrane suspended fillers, as well as the research status and applications of suspended fillers in the field of water treatment are reviewed・ At the same time, the modified research and application on the hydrophilicity, biological affinity and magnetic effect of suspended fillers are introduced・ Discussed the effect of the modification of suspended fillers on the properties of fillers, and made a prospect for the future development of suspended fillers. [ABSTRACT FROM AUTHOR]

BACKGROUND: Partial meniscectomy can effectively reduce the mechanical symptoms of degenerative medial meniscus posterior root tear, but it cannot prevent the progression of knee osteoarthritis, which may be related to the effect of lower limb force line on prognosis. OBJECTIVE: To investigate the correlation between the progression of knee osteoarthritis and the hip-knee-ankle angle in patients with degenerative medial meniscus posterior root tears after partial meniscectomy. METHODS: The medical records of 138 patients with degenerative medial meniscus posterior root tears treated by partial meniscectomy in 904 Hospital of the Joint Service Support Force of Chinese PLA from January to December 2014 were analyzed retrospectively. The average follow-up was (5.53±0.58) years. Patients were divided into group A (hip-knee-ankle angle > 176.2°, n=77) and group B (hip-knee-ankle angle ≤ 176.2°, n=61) according to the best truncation value of hip-knee-ankle angle before operation. Receiver operator characteristic (ROC) curve was used to analyze the predictive value of preoperative hipknee-ankle angle to postoperative American special surgery hospital score HSS. Spearman correlation analysis was used to explore the correlation between preoperative hip-knee-ankle angle and postoperative final HSS value. The width of knee medial joint space and Kaglan-Lawrence classification were compared between the two groups. The trial was approved by the Ethics Committee of 904 Hospital of the Joint Service Support Force of Chinese PLA (approval No. 2018- 10-002). RESULTS AND CONCLUSION: (1) The ROC curve showed that the preoperative hip-knee-ankle angle had a certain predictive value to the postoperative American special surgery hospital score HSS, and the area under the curve was 0.75. (2) Univariate Logistics regression analysis results showed that hip-kneeankle angle and preoperative course of disease were influencing factors for postoperative HSS score. Multivariate Logistics regression analysis results showed that preoperative hip-knee-ankle angle was an independent risk factor for HSS score (P < 0.01), while body mass index and preoperative course of disease did not affect the correlation between hip-knee-ankle angle and HSS score. (3) Spearman correlation analysis results showed that the preoperative hip-kneeankle angle was positively correlated with postoperative HSS score (r=0.472, P < 0.01). The greater the preoperative hip-knee-ankle angle and the higher the preoperative HSS score, the better the prognosis. (4) The width of postoperative joint space in group A was larger than that in group B (P < 0.01), and the degree of Kaglan-Lawrence grade in group A was lower than that in group B (P < 0.05), indicating progression of postoperative osteoarthritis in group A was slower than that in group B. (5) The results showed that for patients with degenerative medial meniscus posterior root tears, partial meniscectomy was not suitable if the hip-knee-ankle angle was ≤ 176.2°. Partial meniscectomy could be performed if the hip-knee-ankle angle was > 176.2°, but relevant risk factors should be carefully considered. [ABSTRACT FROM AUTHOR]

In order to obtain accurate identification and real-time monitoring of mesh damage location, a new modeling method for mesh damage location based on acceleration measurement was proposed by combining physical test and numerical simulation. The method increases the data difference between the monitoring points in the broken area and the non-broken area, so as to determine the location of the broken fishing net. The fishing net physical tests and numerical simulation calculations were combined to study the fishing net breakage localization. Firstly, we carry out the physical model test of mesh coat damage, validate the numerical model of mesh coat, and use the validated numerical model of mesh coat to deeply explore the influencing factors affecting the modeling of mesh coat damage location detection, which serves as the basis of the mesh coat damage location detection model proposed in this study. The damage localization model includes four monitoring areas, and eight damage conditions are listed to test the localization detection ability of the model. The model uses artificial neural networks to localize and identify the damages. The Y-direction acceleration data collected from the monitoring points in the four regions were processed as inputs, and the eight types of damage and one intact case were used as outputs. The training data are the data obtained from numerical simulation and the test data are the sensing data from the physical model. (1) The results show that under the effect of regular waves, when the center of the mesh line is selected as the monitoring point, the data of the monitoring points in the broken and non-broken areas almost overlap, and the difference is within 1%. When the mesh node of the mesh coat is used as the monitoring point, the data change trends of the monitoring points in the two monitoring regions are different, and the data peak and change range of the monitoring point in the broken region at 210-240 s are three times of that of the monitoring point in the non-broken region. (2) Ydirection acceleration component comparing the acceleration, in addition to this broken when the data difference changes the same, with the increase of the degree of broken, the acceleration data changes in the intact state in the range of 0. 75-1. 2 times, while the Y-direction acceleration changes in the range of 0. 75- 23 times, and comparing the other direction of the acceleration component, the intact region and the broken region of the data difference is greater. (3) The accuracy of the model in locating the breakage in the training set is 98. 5 % and the test accuracy is 97. 9 %, which can locate the breakage accurately. In this study, we proposed a modeling method for detecting breakage of mesh garments based on region division, proved that using mesh nodes as monitoring points can increase the difference between monitoring points that are in the intact and broken regions, and the selection of acceleration component as an indicator parameter can increase the ability of the monitoring points to perceive the breakage. The results of this study provide some guidance for the modeling of digital twin of net clothing, and at the same time provide theoretical basis for the sensor arrangement and type selection of fishing nets. [ABSTRACT FROM AUTHOR]

In order to improve the hydrodynamic characteristics of aquaculture tanks with top and bottom compartments and low diameter-to-depth ratio, so as to improve the welfare of aquaculture animals and the economic benefits. In this study, a new structural optimization parameter was proposed, namely the parameter of the bend ratios a/ B(a/ B, a is the corner wall side length, B is the culture liquid bulkhead side length), to evaluate the improvement effect of the flow field inside the aquaculture tank. Based on computational fluid dynamics (CFD) simulation technology, the effects of the parameter of the bend ratios a/ B (0-0. 40) and water inlet velocity (0.8- 1.2 m/s) on the hydrodynamic characteristics in the aquaculture tank were studied. Firstly, in order to verify the correctness of the numerical model and the accuracy of the simulation results, a physical test model of the square aquaculture tank was established, and a numerical model of equal proportions was constructed. Next, the numerical simulation results of the water flow velocity in the aquaculture tank were compared with the test results of the physical model. On this basis, a 40 : 1 scale was carried out on the prototype of the Japan Bulk Carrier to construct a numerical model of the aquaculture ship. Next, the flow field under 9 bend ratios a/ B and 3 inlet velocity conditions of the aquaculture tank system were numerically simulated. Under the same inlet velocity, the hydraulic retention time of each tank was basically equal by adjusting the number of water inlets, so as to quantitatively analyze the influence of the bend ratios a/ B on the hydrodynamic of the aquaculture tank. (1) The average flow velocity v in the tank increases with the increase of the bend ratios a/ B, and the trend is the same under the three inlet velocities. When the bend ratios a/ B=0. 25-0. 40, the average flow velocity of each section in the aquaculture tank is 35% higher than that of the original square aquaculture tank, and the velocity of each section is large and evenly distributed, and there is no obvious low flow velocity area. (2) The uniform distribution coefficient γA in the aquaculture tank decreases first and then increases with the increase of the bend ratios a/ B. When the the bend ratios a/ B=0- 0.1 and 0.25-0.40, the uniform distribution coefficient γA is increased by 7% compared with a/ B= 0.1-0.25.(3) The energy utilization coefficient ηe in the aquaculture tank increases with the increase of the the bend ratios a/ B. When the bend ratios a/ B=0.3- 0.40, the energy effective utilization coefficient ηe is 70% higher than that of the original square aquaculture tank. (4) With the increase of the bend ratios a/B, the vortex column in the center of the aquaculture tank gradually stabilizes, so that the water can move regularly in the tank, and a large vortex ring gradually appears. From the color shading of the velocity, it can be seen that the flow velocity is higher near the tank wall. In aquaculture tanks with smaller bend ratios a/ B, large-scale vortex structures break down into many smaller-scale structures, vortex filaments, and irregular vortex distributions. The aquaculture tanks with the bend ratios a/B in the range of 0.25-0.40 have the characteristics of high average flow velocity, uniform flow field distribution, and no low flow velocity area. They also have a good water environment, high energy effective utilization rate and efficient utilization of aquaculture space. The results of this study have certain guiding significance for optimizing the design of aquaculture tank systems on aquaculture vessels and improving production efficiency. In practical applications, the flow field environment inside the aquaculture tanks can be adjusted based on the bend ratios a/B to maintain good water quality, improve aquaculture efficiency, and reduce operational costs. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of escitalopram combined with psychotherapy on sleep quality, quality of life and serum neurotransmitter levels in patients with anxiety disorder. Methods: 117 patients with anxiety disorder admitted to our hospital from July 2017 to March 2020 were selected, and randomly divided into control group (58 cases) and study group (59 cases). The control group was treated with escitalopram, and the study group was combined with psychotherapy on the basis of the control group. The efficacy, Hamilton Anxiety Scale (HAMA) and Pittsburgh sleep quality index (PSQI) and short form of health survey (SF-36) scores, serum neurotransmitter levels [5-hydroxytryptamine (5-HT), norepinephrine (NE), myeloperoxidase (MPO), neuropeptide (NPY)] and adverse reactions were compared between the two groups. 8 weeks after treatment, the clinical total effective rate of the study group was 88.14% (52/59), which was significantly higher than that of the control group 70.69% (41/58)(P<0.05). Methods: 8 weeks after treatment, the MPO levels of the study group were lower than those of the control group, and the 5-HT, NE and NPY level was higher than that of the control group (P<0.05). 8 weeks after treatment, HAMA and PSQI scores of the study group were lower than those of the control group (P<0.05). 8 weeks after treatment, the scores of mental health, physiological function, physical pain, social function, emotional function, vitality, physiological function and health status of the study group were higher than those of the control group (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). Conclusion: Escitalopram combined with psychotherapy has good efficacy and safety in the treatment of patients with anxiety disorder. It can effectively improve the anxiety symptoms, sleep quality and quality of life, and effectively regulate the serum neurotransmitter levels. [ABSTRACT FROM AUTHOR]

精使用摩務(AUD)是常见的或些性疾病,对个人的身体,认知和社会功能造成不良后果。磁共振成像技术 可无评估 AUD 者脑結梅、功能、代谢及血流动力学改变,就察 AUD 的动态过在,从最初的曲到戒酒及复发,并用于 治疗的研究,为AUD患者认知损伤的神经机制提供了新的见解, 现已广泛应用于临床。本文就近年来藏共振成像技术在 AUD 患者相关的改变的主要研究及进展进行综述. [ABSTRACT FROM AUTHOR]

Objective To establish a clinical anatomic composite grading system and to evaluate the combined effects of the European Heart Surgery Risk Scoring System (EuroSCORE) Ⅱ and SYNTAX scoring system in predicting the prognosis of patients undergoing coronary artery bypass grafting (CABG). Methods The perioperative data of CABG patients in Tianjin chest hospital from July 2017 to December 2018 were retrospectively analyzed. EuroSCOREⅡ and SYNTAX were used to stratify the cardiovascular risk of patients. According to the classification of the two systems, they were arranged and redistributed to construct the clinical anatomy composite grading system. The discrimination and calibration of the system were evaluated compared by EuroSCOREⅡ and SYNTAX. Results The actual hospital mortality for 301 patients was 1.54% (20/1 301). According to the comparison of baseline data after grading by scoring system, there were significant differences between EuroSCORE Ⅱ groups except unstable angina pectoris and SYNTAX. There were significant differences in age, unstable angina pectoris and 30-day death between SYNTAX groups. There were significant differences in other variables except unstable angina pectoris, NYHA and peripheral arterial disease between composite grading system groups. The results of H-L goodness of fit test showed that EuroSCORE Ⅱ, SYNTAX and composite grading system had good fitting degree. ROC curve analysis showed that the accuracy of composite classification system was the best, (AUC=0.862). AUC of EuroSCORE Ⅱ and SYNTAX were 0.849 and 0.732 (P＜0.01). This indicates that the composite grading system can significantly improve the predictive ability of 30-day mortality after operation. Compared with EuroSCORE Ⅱ, NRI of composite grading system was 14.05% (Z=7.205, P＜0.01), compared with SYNTAX, NRI=37.85% (Z=4.240, P＜0.01). Conclusion The composite classification system of EuroSCORE Ⅱ and SYNTAX can improve the ability of single type scoring system to predict the short-term mortality of CABG. [ABSTRACT FROM AUTHOR]

Travel note Fu has its unique features in Han Fu with the description of journey. It was found that travel note Fu inherited some characteristics and form of Chu Ci. In this paper,the inheritance of travel note Fu to Chu Ci in aspects such as the theme,the conclusion form with "乱曰"at the end as well as the use of "兮",the vocabulary and the chilly and deep style are discussed. [ABSTRACT FROM AUTHOR]

The large-scale data computig process includes different modes which are streaming computing, memory computing, batching computing and graph computing mode. Each mode has different access memory, communication and resource utilization. GPU clusters are widely used in large-scale data processing. However, there is lack of computing model for GPU cluster in large-scale data process. The collaborative computing between GPU and CPU not only increases density of computing resources but also improves communication complexity between inter-nodes and intra-nodes. To explore rule of collaborative computing process, this paper built a novel multi-stage collaborative computing model ( p-DCOT ) for multi-computing modes . This model was based on ESP model, which was large synchronous parallel model. It divided the collaborative computing process into three levels: data layer, computing layer and communication layer. This model also used matrix of DOT model to describe computing and communication behaviors. Then it extended the p-DOT model to describe collaborative computing behavior within computing nodes and refined the parameters of workloads balancie. This model could prove the time cost function of collaborative computing model. Finally, the typical computing tasks verified the model validity and parameter analys is. This collaborative computing model will be a tool to reveal collaborative computing behaviors in large-scale data analysis processing. [ABSTRACT FROM AUTHOR]

Objective To systematically evaluate the long-term efficacy and complications of hybrid coronary artery revascularization (HCR) and coronary artery bypass grafting (CABG) in the treatment of the multiple coronary artery disease. Methods The databases of Embase, PubMed, Web of Science, Cochrane Central Registry of Controlled Trials (Central), Wanfang Data and China Knowledge Network were systematically searched. The documents that met the inclusion criteria were screened, and the odds ratio (OR) and 95% confidence interval (CI) were calculated. RevMan 5.3 software was used for meta-analysis. Results Nine articles were included in the study. A total of 4 030 subjects were included, of which 1 142 were treated with HCR and 2 888 were treated with conventional CABG. Postoperative mortality (OR=0.72, 95%CI: 0.54- 0.96) and major cardiovascular and cerebrovascular events (MACCE, OR=0.54, 95%CI: 0.35-0.82) were lower in HCR group than those of CABG group in the mid- and long-term follow-up. There were no significant differences in the revascularization (OR=0.90, 95%CI: 0.61-1.34) and the incidence of angina (OR=0.51, 95%CI:0.18-1.41) between the two groups. In the midterm follow-up, the incidence of MACCE was lower in the HCR group than that in the conventional group (OR=0.31, 95%CI: 0.15-0.66), while there were no significant differences in the mortality (OR=0.79, 95%CI: 0.52-1.22), revascularization (OR=0.90, 95%CI: 0.61-1.34) and the incidence of myocardial infarction angina (OR=0.71, 95%CI: 0.05- 9.46) between the two groups. In the long-term follow-up, the postoperative mortality (OR=0.67, 95%CI: 0.54-0.96) and the incidence of myocardial infarction angina (OR=0.32, 95%CI: 0.15-0.68) were significantly lower in HCR group than those of the CABG group. There were no significant differences in MACCE (OR=0.72, 95%CI: 0.43-1.21) and revascularization (OR= 0.93, 95%CI: 0.60-1.45) between the two groups. Conclusion Compared with CABG, HCR can reduce the medium-term MACCE, long-term all-cause mortality and myocardial infarction/angina pectoris in patients with multi-vessel coronary artery disease. [ABSTRACT FROM AUTHOR]

To improve the high temperature stability of asphalt mixture, the three-axis numerical test method of asphalt mixture was constructed by partical flow code in 3 dimensions (PFC3D), and the reliability was evaluated by indoor triaxial test. The effects of coarse aggregate grading, fine aggregate grading and coarse aggregate ratio on the shear strength of asphalt mixture were investigated by numerical triaxial test and laboratory test. The AC-20 mineral gradation was proposed based on the shear strength, and the road performance was verified according to the effects of 19.000, 4.750, 0.075 mm mesh pass rates on AC-20 performance. The results show that the three-axis numerical test of asphalt mixture has good similarity with the indoor test. When the volume ratio of coarse aggregates of 19.000~26.500，16.000~19.000，13.200 ~16.000，9.500~13.200 and 4.750~9.500 mm is 4 ∶12 ∶4 ∶10 ∶15, and the fine aggregate takes the corresponding gradation for I equaling to 0.75 with the volume ratio of coarse aggregate of 60 ∶40, the asphalt mixture has the highest shear strength. The coarse aggregates are recommended to be 19.000, 4.750 and 0.075 mm with the pass rate ranges of 90.0%~100.0%, 35.0%~45.0% and 4.0%~7.0%, respectively, and the AC-20 optimized gradation is proposed based on shear strength. Compared with the standard gradation, the shear strength and the dynamic stability of the optimized AC-20 gradation are increased by 26% and 29%, respectively. [ABSTRACT FROM AUTHOR]

To d eal with the low resource utilization of virtual cluster due to the time-varying and complexity of workloads, this paper used the flexible resource management strategy to improve the global resource utilization of virtual cluster and absorb the mutation of resource demand when multiple computing modes were mixed. This paper proposed a novel dynamic deployment mod el based on Docker. According to the dynamic demand of workloads, this model could change the computing form of virtual cluster in real time. Its computing form included the node type and cluster size. This dynamic deployment model not only realized the dynamic customization of j ob execution environment, but also achieved the purpose of virtual cluster peak calculation. Simulation experiments show that this model can improve the virtual node CPU utilization by 5. 3% and improve the task execution efficiency. This model is suitable for data centers or large-scale clusters to improve the utilization of computing resources . [ABSTRACT FROM AUTHOR]

BACKGROUND: The intraoperatlve blood loss of unicompartmental knee arthroplasty is lower than that of total knee arthroplasty, but the difference of严!operative occult blood loss between them has not been widely studied. OBJECTIVE: To compare the difference of occult blood loss between unicompartmental knee arthroplasty and total knee arthroplasty, and to analyze whether patients with preoperative anemia can also undergo unicompartmental knee arthroplasty without the risk of blood transfusion. METHODS: 148 patients with knee osteoarthritis treated in 904 Hospital of the Joint Service Support Force of Chinese PLA from January 2014 to December 2016 were selected. Among them, 58 cases underwent unicompartmental knee arthroplasty and 90 cases underwent total knee arthroplasty. Hemoglobin levels and hematocrit were measured before and 1, 4, 21 and 42 days after operation, and the occult blood loss and blood transfusion rate were calculated. The trial was approved by the Ethics Committee of 904 Hospital of the Joint Service Support Force of Chinese PLA (approval No. 2019-01-03). RES UL TS AND CONCLUSION: (1) The hemoglobin level of unicompartmental knee arthroplasty group was higher than that of total knee arthroplasty group at 1 and 4 days after operaation (P < 0.01). There was no significant difference in hemoglobin level between the two groups before operation and 21, 42 days after operation (P > 0.05). (2) Hematocrit in the unicompartmental knee arthroplasty group was higher than that in the total knee arthroplasty group at 1 and 4 days after operation (P < 0.01). There was no significant difference in hematocrit between the two groups before operation and 21, 42 days after operation (P > 0.05).(3) From day one to day four after surgery, there was no occult blood loss in the female of unicompartmental knee arthroplasty group, and the average occult blood loss in the male was 4 g/L. The average occult blood loss was 1 O g/L in female and 7 g/L in male in total knee arthroplasty group. The occult blood loss in male and female in unicompartmental knee arthroplasty group was lower than that in total knee arthroplasty group (P < 0.05 or P < 0.01). (4) The blood transfusion rate was 0% in unicompartmental knee arthroplasty group and 4.4% in total knee arthroplasty group. In patients with preoperative moderate anemia (hemoglobin 60-89 g/L), there was no need for blood transfusion in unicompartmental knee arthroplasty group (n=3), but blood transfusion was needed in 2 of 6 patients in total knee arthroplasty group (33%). (5) The results showed that unicompartmental knee arthroplasty had obvious advantages over total knee arthroplasty in postoperative occult blood loss. [ABSTRACT FROM AUTHOR]

Semantic similarity measurement is the key technology to realize the integration and fusion of multi-source vector spatial data. Firstly, this paper analyzes and describes the semantic information of geographical entities from the perspective of vector spatial data representation, and proposes a semantic similarity measurement model based on multi-feature constraints.Secondly,the model uses the classification relation of geographical elements to control the extraction of the target entity set. On the basis of constructing the corresponding relationship of semantic features among entities, the concept of attribute feature entropy is introduced to calculate the weight values of different features, and then the overall semantic similarity of geographical entities is measured by synthesizing the multi-feature similarity. Finally, the model is applied to road entity matching. The road matching is realized by calculating the semantic similarity between entities. Meanwhile, the validity of the model is verified. The experimental results show that the semantic similarity measurement model based on multi - feature constraints can reasonably calculate the semantic similarity of geographical entities and improve the efficiency of semantic matching of geographical entities. [ABSTRACT FROM AUTHOR]

Objective To explore the therapeutic effect of verbascoside（ Ver） combined with 99Tc-methylenediphosphate( 99TcMDP) on glucocorticoid-induced osteoporosis（ GIO） in rats and its molecular mechanism.Methods SD rats received muscular injection of dexamethasone（ Dex） sodium phosphate（ 1 mg/kg,twice a week） for 8 weeks to establish GIO model.After GIO model was established,rats in Ver group,combined group,99Tc-MDP group,and model group received Ver（ 30 mg/kg）,Ver combined99Tc-MDP（ 5 mg/kg） 99Tc-MDP（ 5 mg/kg）,or normal saline,respectively.Rats in the control group were not modeled.The related indexes were detected after 8 weeks of the drug administration.Results Compared with those in rats of the control group,the body weight and weight of the femoral shaft in rats of the model group reduced significantly,and the blood indexes related to bone metabolism were obviously abnormal.The result of dual-energy X-ray absorption（ DXA） showed that bone mineral density of the whole body,the lumbar spine,and the femur in the model group decreased significantly.The maximum load,elastic load,elastic displacement,and elastic modulus of the three-point bending test of the femur also reduced significantly.RT-PCR result showed that the expressions of USP10 and FLT3 in the bone tissue of the model group were higher than those in the control group.The above symptoms were relieved after the treatment with Ver and 99Tc-MDP.Further study showed that USP10/FLT3 expression in the Ver group and the combination group was significantly lower than that in the 99Tc-MDP group and the model group,but there was no significant difference between the99Tc-MDP group and the model group.Conclusion Ver inhibits the activity of USP10/FLT3 pathway in rat bone tissue and relieves GIO.Ver and 99Tc-MDP have a good pharmacological synergistic effect on the treatment GIO rats.This is accomplished by acting on different molecular targets. [ABSTRACT FROM AUTHOR]

To optimize communication efficiency and reduce the data transmission of shuffle in large-scale clusters running for MapReduce jobs, this paper built a distributed collaborative data mapping model by replacing the communication locality using storage locality. Then it extracted the local features of jobs by random sampling and machine learning method in order to realize the effective deployment of map tasks. Finally, it selected the nodes with good communication links based on the software define network technology due to its global flexible control capabilities, and scheduled the map tasks to such nodes. Experimental results show that the model has better optimization effect on shuffle-intensive jobs. The communication delay is reduced by 4.3% to 5.8% . This solution can reduce shuffle traffic and data migration delay and it is suitable for various scheduling strategies and network topologies. [ABSTRACT FROM AUTHOR]

Firstly, Ni-Mn-Ga-Co alloy powders with different particle sizes were prepared b y ball milling. Then, porous Ni-Mn-Ga-Co magnetic shape memory alloy with foamed structure was successfully prepared by 3D printing technology. Microstructure, phase structure, phase transformation and related magnetic properties of the alloy were studied by means of SEM, DSC and XRD. The results showed that the alloy powders with different particle sizes obtained by ball milling and sieving were irregular in shape. Ni-Mn-Ga-Co alloy powder had a non-modulated tetragonal martensite structure at room temperature, and its characteristic peak wasvery obvious. The DSC curves of Ni-Mn-Ga-Co alloys showed wide-peak phase transition, and the add it ion of Co had little effect on the initial martensitic transformation temperature (Ms), but the Curie temperature (Tc) of Ni Mn-Ga-Co alloys had a significant increase. The maximum saturation magnetization of the magnetic alloys prepared by sintering with 50-100 µm alloy powder was 68 Am² / kg. The smaller the particle size of the alloy powder, the higher the density of the sintered porous Ni-Mn-Ga-Co magnetic shape memory alloy. When the particle size of alloy powder was less than 50 µm, the density could reach 90 %, and when the particle size of alloy powder was 50-100 µm, the density was only 75 % . Compared with the alloy powder with smaller particle size, the magnetic alloy produced by the alloy powder with larger particle size had higher magnetic induction strain capacity, which was because the foam structure could effectively reduce the internal and external constraints, thereby improving the induced strain of magnetic field. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of steroid coenzyme A desaturase 1(SCD1) overexpression on osteogenic differentiation of bone marrow mesenchymal stem cells(BM-MSCs), and to analyze the changes of whole genome expression profile by gene chip technology. Methods: BM-MSCs were transfected with SCD1 lentivirus. The overexpression and activity of SCD1 in BM-MSCs were detected by RT-PCR and C14 technique. OC and other related osteogenesis indexes were detected by Western blot and alizarin red staining after BM-MSCs were induced and cultured. The effect of overexpression of SCD1 on osteogenic differentiation expression profile of BM-MSCs was further examined by whole gene chip. Results: SCD1 was successfully overexpressed in BM-MSCs,and SCD1 activity in overexpressing group was significantly higher than that in control group. The levels of alkaline phosphatase(APL)and osteocalcin(OC) in the overexpression group were higher than those in the control group at 7 and 14 days after osteogenesis induction(P < 0.05). Alkaline phosphatase staining and alizarin red staining were more in the overexpression group than in the control group at 7 and 14 days after osteogenesis induction. The results of gene expression microarray showed that 2896 differentially expressed genes were detected by overexpression of SCD1 in bone marrow mesenchymal stem cells. Gene pathway analysis showed that the interferon pathway was the most significant pathway(P<0.05). Conclusion: Overexpression of SCD1 can promote the osteogenic differentiation of BM-MSCs, which may affect the osteogenic differentiation function through interferon pathway. This finding may provide important thinking and potential therapeutic strategies for fracture healing, and is worthy of further study. [ABSTRACT FROM AUTHOR]

Objective: To clarify the pathophysiological correlation between alpha-nuclear synaptic protein and Parkinson's disease and its clinical significance. Methods: Chromatography - mass spectrometry (UPLC -ms) detection of wild type mice and endogenous metabolic product of gene mutant mice brain tissue by mzcloud endogenous metabolic substances identified in brain tissue in mice, the corresponding data of principal component analysis (PCA) and cluster analysis, analysis of the relevant differentially expressed metabolites, and construct the circuit diagram and interaction network diagram. Results: (1) the results of metabolism analysis based on LC/MS method showed that the metabolites between the two groups were mainly amino acids and phospholipids, including beta-alanyl-histidine, l-arginine, l-histidine, l-leucine, l-phenylalanine, l-valine, l-aspartic acid, l-alanine, phospholipid choline, etc. (2) build the metabolic pathways of mainly relates to the synthesis and degradation of ketone body, taurine and the metabolism of taurine, alanine, glutamic acid and aspartic acid metabolism, arginine and proline metabolism, histidine metabolism, phenylalanine metabolism, valine, leucine and isoleucine biosynthesis, glycerol phospholipid metabolism, etc., find 18 landmark metabolic components. Conclusion: after alpha nuclear synaptic protein gene mutations, ketone body of synthesis and degradation, taurine and the metabolism of taurine, alanine, glutamic acid and aspartic acid metabolism, arginine and proline metabolism, histidine metabolism, phenylalanine metabolism, valine, leucine and isoleucine biosynthesis and glycerol phospholipid metabolism in the changes of metabolic pathways, involved in beta - propylene ammonia acyl - L - histidine, L - arginine, L - histidine, L leucine, L - phenylalanine, L - valine, L - aspartate, L - alanine, phosphatidyl choline etc. Change the biological signature of metabolites. [ABSTRACT FROM AUTHOR]

[Objectives] This paper aimed to study the nematicidal activity of the Pseudomonas protegens Pf-5, and to identify the major nematicidal compounds produced by this strain. [Methods] The unmarked deletion mutants of Pf-5 were constructed based on sucrose-induced(sacB gene)lethalality. The model nematodes Caenorhabditis elegans was used as the target. The strains were labeled with green fluorescent protein gene to study the lethal effect of the strain on this nematode. The possible action site of Pf-5 strain was observed by using scanning and transmission electron microscopy. [Results] The Pf-5 strain had strong lethal activity against C. elegans. The LCM of Pf-5 against C. elegans at 48 h and 96 h.was respectively 4.92xl07 CFU·mL-1 and 3.58xlO6 CFU·mL-1. When treated C. elegans with Pf-5 for 48 h, the lethal rate was 73.57%,but when Jit I) gene was knocked out, the lethal activity of PfSufUD was significantly reduced, only 25.04% compared to the wide type. When Delivery domain and CHOPS domain of July gene were respectively deleted, the lethal activity of mutants also significantly decreased. This indicated that the FiteD protein was the major nematicidal substance produced by Pf-5 strain. Scanning electron microscopy showed that Pf-5 strain and its mutant did not destroy the body wall of nematode. But when observed using transmission electron microscopy,the results had showed that the intestinal villi of nematode became thinner, and even fell off, but the mutant Pf-5μfiD didn't have such effect. [Conclusions] The nematicidal substance in the P. protegens Pf-5 strain is the FitD protein,and its site of action is the intestinal tract. [ABSTRACT FROM AUTHOR]

[Objectives]The aim of this experiment was to investigate the protective effect of retinoic acid on testicular injury in heat stressed mice. [Methods]Forty 7-week-old male ICR mice were randomly divided into four groups:control group(CON), heat stressed group(HTRA0), 5 mg·kg-1·d-1retinoic acid-treated group(HTRA5)and 10 mg·kg-1·d-1retinoic acid-treated group(HTRA10). Mice were gavaged with appropriate doses of retinoic acid or corn oil and then subjected to systemic heat stress at 42 ℃ for 2 h. The mice were slaughtered and sampled at the end of the test. [Results]Compared with the CON group, the testicular weight, minimum diameter, cross-sectional area, diameter, and epithelial height of spermatogenic tubules in the HTRA0 group were significantly reduced(P<0.05), the epididymal sperm motility significantly decreased(P<0.05), the malondialdehyde(MDA)content and the expression levels of antioxidant related genes(nrf2, ho-1, nqo1, sod1, gpx1)in testis tissue significantly increased(P<0.05), while total superoxide dismutase(T-SOD)activity significantly decreased(P<0.05), the mRNA expression level of bax significantly increased(P<0.05), the expression levels of blood-testis barrier related genes(occludin, zo-1, n-cadherin and connexin 43)significantly increased(P<0.05), and the expression levels of spermatogenesis related genes(plzf, c-kit)expression significantly increased(P<0.05). Compared with HTRA0, the cross-sectional area, diameter and epithelial height in the HTRA5 group significantly increased(P<0.05), the MDA content significantly decreased(P<0.05)while T-SOD activity significantly increased(P<0.05), and the mRNA expression levels of plzf and c-kit significantly decreased(P<0.05). Compared with HTRA0, the cross-sectional area, diameter and epithelial height of spermatogenic tubules in the HTRA10 group significantly increased(P<0.05), sperm motility significantly increased(P<0.05), MDA content significantly decreased(P<0.05), T-SOD activity significantly increased(P<0.05), and the mRNA expression levels of nqo1, bax, bcl-2, zo-1, n-cadherin, plzf and c-kit were significantly reduced(P<0.05). [Conclusions]Retinoic acid could improve testicular weight, testicular histomorphology, and epididymal sperm motility in heat-stressed mice. This protective effect may be achieved by increasing the antioxidant capacity of mouse testes, reducing germ cell apoptosis, enhancing the blood-testis barrier, and improving the spermatogenic microenvironment. [ABSTRACT FROM AUTHOR]

Liver cirrhosis is a progressive chronic liver disease due to one or more causes, with diffuse fibrosis in liver tissue, pseudolobules, and regenerative nodules as major histological features. Once liver cirrhosis enters the decompensated stage, the liver and several other organs are injured, which can hardly be recovered or reversed. This article introduces the process of Toll — like receptors in recognizing the changes in intestinal flora and the influence of this process on the development and progression of liver cirrhosis, as well as the protective effect of bile acid against liver cirrhosis by regulating intestinal flora. This article also reviews the advances in delaying liver cirrhosis after the treatment of intestinal dysbacteriosis and points out that the treatment of intestinal dysbacteriosis may become the major direction of liver cirrhosis treatment in future. [ABSTRACT FROM AUTHOR]

The performance of satellite gravimetry is determined not only by orbital parameters, sensitivity of payloads, resolution of data, et al, but also by inaccuracy of the Earth gravity recovered methods. In past years, the performance analysis results were unavoidably affected by the mathematical model error from recovering methods such as dynamic method, short-arc integrated method, et al. To solve this problem, space-wise least square method is present. The effects of each items which affected the performance of satellite gravimetry are evaluated by this method. The results indicate that the highest degree of the earth gravity model recovered is 240 for the satellite with 500 km orbital height. Then if the error of payload is 1×10-10 m·s-2·Hz-1/2, the degree of model recovered only approach to 136 with the accumulated gravity anomaly error 2.7 mGal and the accumulated geoid height error 14 cm. In order to achieve the best surveying performance, the orbital inclination should be greater than 89°. While, the max degree of the earth gravity model recovered should be greater than 240 so as to reduce the effect of high frequency gravity signal on low degree coefficients recovered. All these conclusions benefit to satellite designing and data processing. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of lactobacillus acidophilus on the expression of nitric oxide (NO) and its oxidative mediators, and to explore the anti-atherosclerosis mechanism of lactobacillus acidophilus. Methods Twentyfour male SPF rats were randomly divided into normal diet group, high-fat diet group and lactobacillus acidophilus group, with 8 rats in each group. The rats in the normal diet group were fed with normal diet, and the high-fat diet group was fed with high-fat diet + intraperitoneal injection of vitamin D3 + immune injury + FeSO4 to establish an atherosclerosis model. The rats in the lactobacillus acidophilus group were intragastrically administered with 0.5 mL of lactobacillus acidophilus per day (1×109 CFU/mL) on the basis of the high-fat diet. Rats were weighed at the 4th, 8th and 12th weekends. After 12 weeks of feeding, the serum was taken from abdominal aorta to detect the contents of oxidized low density lipoprotein (oxLDL), NO, arginine, arginase, and peroxynitrite (ONOO-). The aorta was isolated to observe the morphological change by HE staining. The expressions of eNOS and iNOS mRNA were detected by Real-time PCR, and the expressions of NF-κB p65 subunits were detected by Western blot assay. Results (1) There was no significant difference in body weight between the high-fat diet group and the lactobacillus acidophilus group during the whole experiment (P＞0.05), but both were significantly increased compared with those of the normal diet group (P < 0.01). (2) HE staining showed that the aorta formed a wide range of atherosclerotic lesions in the high-fat diet rats, and the aortic morphology was significantly improved in the lactobacillus acidophilus group. Only endothelial cell proliferation was found, and no smooth muscle cell necrosis was observed in this group. (3) Compared with normal diet group, the serum expressions of oxLDL, ONOO- , arginase, aortic iNOS mRNA and nuclear NF-κB p65 were increased in high-fat diet group (P＜0.01), and the serum arginine content and aortic eNOS mRNA expression levels were decreased (P＜0.01). While lactobacillus acidophilus can just reverse the above changes (P＜0.01). Conclusion Lactobacillus acidophilus may exert anti-atherosclerotic effects by regulating the expression of NOS, increasing NO bioavailability, and protecting endothelial function. [ABSTRACT FROM AUTHOR]

Objective: To explore the potential mechanism of celastrol in inflammatory bowel disease (IBD) by investigating its effects on the serum bile acid metabolite profiling of IBD mice . Methods ： The IBD mice model was developed by using dextran sulfate sodium . The relative concentrations of 22 bile acids in the serum of IBD mice were determined by using U P L C - M S / M S . Results ： In EBD mice，the relative concentrations of free primary bile acids，such as cholic acid (C A) and chenodeoxycholic acid (C D C A) were significantly increased，while the levels of several binding primary bile acids，such as tauroc ho lie acid (T C A) and taurodeoxycholic acid (T D C A) were obviously decreased，and the levels of some secondary bile acids， such as lithocholic acid (L C A) were also significantly reduced .After administration of celastrol，the metabolic changes of the above primary and secondary bile acids in the serum of the IBD mice recovered to a certain extent .Conclusion ：Celastrol might regulate the liver function and metabolic enzyme expression of the IBD mice，intervene the binding process of primary bile acids， thus improve the symptoms of the EBD mice. [ABSTRACT FROM AUTHOR]

Thirty-two teachers from 3 key middle schools in Harbin have been interviewed on the concept, object, timing, motivation and process of learning evaluation in the course of education, focusing on the extent to which teachers use student learning evaluation discourse and teachers’ evaluation of students’ learning and whether there is a difference between the two. The research aims to understand the extent to which middle school teachers adopt a new language of instruction in the practice of learning evaluation. The results show that teachers will add educational discourse in the practice of learning evaluation by renaming the educational discourse related to learning evaluation, and that the formative evaluation of the use of discourse can help to improve the effectiveness of the teacher education process. [ABSTRACT FROM AUTHOR]

The quartz, bauxite and light burned magnesium oxide are used as the main raw materials which the cordierite glass-ceramics was synthesized by melting method and solid-state reaction method respectively. The structure and performance of the obtained samples were characterized to explore and compare the advantages and disadvantages of the two kinds of processes. The results show that the melting method can precipitate a large number of a-cordierite phase at 950 °C .and the coefficient of thermal expansion is 2. 23 × 10-6°C-1 after crystallized at 1 000 °C for 2 h. However, the solid-state reaction method can precipitate a large number of a-cordierite phase at 1 300 °C . and the coefficient of thermal expansion is 1.46 X 10-6°C-1 after crystallized at 1 350 °C for 2 h. In addition, the synthesis temperature of melting method is nearly 350 °C lower than that of the solid-state reaction,and the range of the synthesis temperature is wide. But for the melting method, its preparation process is more complicated and cost is higher. [ABSTRACT FROM AUTHOR]

Objective: Explored the effect of Stearoyl-CoA desaturase 1 (SCD1) overexpression on the endothelial differentiation function of human bone marrow mesenchymal stem cells (BM-MSCs) and genes expression profile, through the gene chip and intelligent pathway analysis system (IPA). Methods: Used the SCD1 lentivirus which had been successfully built to transfect BM-MSCs. Performed RT-PCR to detect the expression of SCD1 and used ratio of ([ 14C] oleic acid)/ ([14C] oleic and stearic acids) to detected the activity of SCD1. After endothelial induction culture, used RT-PCR to detect the endothelial index including CD31, vWF and CDH5. Gene microarray was performed and used the Ingenuity Pathway Analysis (IPA) system to analyze the differential gene expression profile of the BM-MSCs modulated by SCD1 overexpression. Results: SCD1 was stably overexpressed in BM-MSCs and maintained a highly activity. After 7 days of endothelial induction culture. CD31 and vWF mRXA of overexpression group were higher than that of the control groupp(p< 0.05). and CD31. vWF and CDH5 mRXA of overexpression group were higher than that of the control group after 14 days (p< 0.05). The microarray showed there are total 522 differential expression genes and IPA results show that the genes of NRF2 pathway and the genes of Cell Differentiation Function were significantly differential expression genes (p < 0.05). Conclusion: SCD1 expression can promote the endothelial differentiation of BM-MSCs. probably by reducing the cell oxidative stress and improving the ability of cell proliferation differentiation. This effect of SCD1 may provide the potential strategy for the endothelial function repair and cardiovascular diseases treatment, which is worth further study. [ABSTRACT FROM AUTHOR]

Objective To study the effects of lithium chloride (LiCI) preconditioning on bone loss, JAK/STAT signaling pathway, and osteoblast (OB) in bilaterally ovariectomized mice. Methods In vivo experimental: 24 female 8-week-old C57BL6/J mice were randomly divided into sham operation group (Sham), ovariectomized operation group (OVX), and ovariectomized operation+Lithium chloride intervention (OVX+LiCI) group. The left and right femurs and the right tibia of the animals were collected and tested using micro-CT, immunohistochemistry, and bone biomechanics. In vitro experiment: 3-day-old C57BL/6 mice were sacrificed. The calvarial bone was isolated to culture primary osteoblasts and randomly divided into blank group (Control) and LiCl group (LiCI). Alkaline phosphatase staining, Alizarin red staining, cell activity detection, and real-time quantitative PCR analysis were used. Results Micro-CT analysis result showed that the bone microstructure in the Sham group was generally normal. However, the microstructure in the OVX group changed abnormally, mainly including decreased bone mass, thinning of trabecular thickness, and decreased number of trabecular bone. The degree of separation of trabecular bone was increased. The intervention with LiCl significantly improved bone loss. Biomechanics result showed that the maximum load and maximum stress in mice of the OVX group were significantly lower than those of the Sham group (P<0.05), and the maximum load and maximum stress of the OVX + LiCl group were significantly higher than those of the OVX group (P<0.05). Immunohistochemical staining showed that the expressions of JAK2 and STAT3 in the OVX+LiCl group were significantly higher than those in the OVX group (P<0.05). The expression of P-STAT3 in the OVX group was significantly lower than that in the Sham group (P<0. 05 vs. Sham), while the expression in OVX+LiCl group increased significantly (P<0.05 vs. OVX). The proliferation activity of osteoblasts in the LiCl group was significantly higher than that in the control group (P<0.05). The alkaline phosphatase activity and the number of calcium nodules in the LiCl group were significantly higher than those in the control group (P <0.05). The expressions of STAT3 mRNA and OCN mRNA in LiCl group were significantly higher than those in control group (P <0.05). Conclusion LiCl improves bone mass and bone microstructure of OVX mice by regulating OB. The mechanism may be related to the regulation of the JAK/STAT3 signaling pathway. [ABSTRACT FROM AUTHOR]