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Endometrial receptivity (ER), one of important factors related to the successful implantation of embryo, refers to the ability of endometrium to accept the embryo. Benifited from the development of assisted reproductive technology, the number of high-quality embryos has increased. However, the success rate of embryo implantation has not reached a high level. Most studies on the implantation process are focused on embryo, while the study on endometrial factors is less. It is necessary to pay attention to ER, and how to improve ER, so as to improve the embryo implantation rate and the clinical pregnancy rate. Heparin as an anticoagulant plays an important role in anticoagulant, inhibition of platelet aggregation and anti-inflammation. Heparin was also widely used in the field of obstetrics and gynecology, such as the prevention of postoperative thrombosis and recurrent abortion, and the treatment of gestational hypertension and other diseases. In addition, heparin has also been used to improve the success rate of embryo implantation in the clinical practice of reproductive medicine due to its positive effect on ER. The clinical application of heparin in reproductive medicine, as well as the mechanism related to ER, was reviewed. [ABSTRACT FROM AUTHOR]

To evaluate the application value of serum 25-hydroxyvitamin D [25 (OH)D], anti-Mullerian hormone (AMH) and inhibin B (INHB) in pregnancy outcome of in vitro fertilization-embryo transfer (IVR-ET) in patients with diminished ovarian reserve (DOR). 292 DOR patients who underwent IVR-ET assisted pregnancy in our hospital from August 2019 to October 2021 were selected, according to the pregnancy outcome after assisted pregnancy, they were divided into pregnancy group (n=158) and non-pregnancy group (n=134), the clinical data and the levels of serum 25(OH)D, AMH and INHB in the two groups were compared, the predictive value of serum 25 (OH)D, AMH and INHB in separate and combination detection for pregnancy failure was evaluated by the receiver operating characteristic (ROC) curve. The influencing factors of pregnancy failure were analyzed by single factor analysis and multiple logistic regression. The levels of serum 25 (OH)D, AMH and INHB in pregnant group were significantly higher than those in non-pregnancy group (P<0.05). ROC curve analysis result showed that the area under the curve (AUC) of serum 25(OH)D, AMH, INHB and their combined detection in predicting IVR-ET pregnancy failure in patients with DOR were 0.714 (0.631~0.802), 0.801 (0.731~0.852), 0.634 (0.578~0.741), 0.853 (0.806~0.913) respectively, the AUC of the combined detection in predicting IVR-ET pregnancy failure in patients with DOR was higher than those in the separate detection. Single factor analysis results showed that the number of sinus follicles<5, the number of retrieved eggs<8, follicle stimulating hormone (FSH) / luteinizing hormone (LH)≥ 1.8, serum 25 (OH)≤ 25.05 μg/L, serum AMH≤ 1.08 ng/mL, serum INHB≤ 84.28 pg/ml in the non-pregnancy group were significantly higher than those in pregnancy group (P<0.05). Multiple logistic regression analysis results showed that FSH/LH≥1.8, serum 25(OH)D≤ 25.05 μg/L, serum AMH≤ 1.08 ng/mL, serum INHB≤ 84.28 pg/mL were the risk factors of IVR-ET pregnancy failurein patients with DOR (P<0.05). The low levels of serum 25(OH)D, AMH and INHB are related to IVR-ET pregnancy failure in patients with DOR, and the combined detection of the above index levels has high auxiliary predictive value for IVR-ET pregnancy failure. [ABSTRACT FROM AUTHOR]

Objective: To study the relationship between serum leptin, irisin, sex hormone binding globulin (SHBG) and aquaporin 8 (AQP8) and sex hormone levels and pregnancy outcome in patients with polycystic ovary syndrome (PCOS) infertility undergoing in vitro fertilization-embryo transfer (IVF-ET). A total of 293 patients with PCOS infertility undergoing IVF-ET who were admitted to our Hospital from January 2020 to January 2022 were selected, and the patients were divided into clinical pregnancy failure group (n=118) and clinical pregnancy success group (n=175) according to whether or not the pregnancy was clinically successful. Clinical data of patients were collected, and serum leptin, irisin, SHBG and AQP8 levels were measured. Pearson's correlation coefficient was used to analyze the correlation between serum leptin, irisin, SHBG and AQP8 levels and sex hormone levels in PCOS infertility, multivariate Logistic regression was used to analyze influencing factors of pregnancy outcome in PCOS infertility patients undergoing IVF-ET, and receiver operating characteristic (ROC) curves were used to analyze the value of serum leptin, irisin, SHBG and AQP8 levels alone and in combination to predict clinical pregnancy failure in PCOS infertility patients undergoing IVF-ET. Compared to the clinical pregnancy success group, the clinical pregnancy failure group had lower follicle stimulating hormone (FSH), gonadotropin (Gn) dosage, number of eggs gained, fertilization rate, excellent embryo rate and SHBG levels, and higher luteinizing hormone (LH), LH/FSH, testosterone (T), insulin resistance index (HOMA-IR), leptin, irisin and AQP8 levels. Pearson correlation coefficients showed that serum leptin, irisin and AQP8 were negatively correlated with FSH level, and positively correlated with LH, LH/FSH and T levels in patients with PCOS infertility (all P＜0.05). SHBG was positively correlated with FSH level, and negatively correlated with LH, LH/FSHand T levels (all P＜0.05). Multivariate Logistic regression analysis showed that LH/FSH, T, HOMA-IR, leptin, irisin and AQP8 were independent risk factors for clinical pregnancy failure in patients undergoing IVF-ET for PCOS infertility, and excellent embryo rate and SHBG were independent protective factors (all P＜0.05). The ROC curves showed that the area under the curve (AUC) for serum leptin, irisin, SHBG and AQP8 levels in combination to predict clinical pregnancy failure in PCOS infertility patients undergoing IVF-ET were greater than each indicator alone. Serum leptin, irisin, SHBG and AQP8 levels are associated with FSH, LH, LH/FSH, T levels and clinical pregnancy failure in PCOS infertility patients undergoing IVF-ET, and it is helpful to predict the pregnancy outcome of patients with PCOS infertility. [ABSTRACT FROM AUTHOR]

Objective：To evaluate the clinical application of non -invasive prenatal testing (NIPT) in screening of fetal chromosomal aneuploidy. Methods：Totally 5 756 pregnant women receiving NIPT in People′s Liberation Army No.202 Hospital from December 2014 to December 2016 were retrospectively analyzed. Amniocentesis and fetal karyotyping were recommended in those women with the high -risk results of NIPT. Results：Among 5 756 pregnant women, 62 cases were found the high-risk by NIPT, with a positive rate of 1.08%. 48 cases underwent chromosome karyotype analysis, including 24 cases with trisomy 21, 2 cases with trisomy 18, 1 case with trisomy 13, and 8 cases with sex chromosome abnormality. The positive predictive values of trisomy 21, trisomy 18, trisomy 13 and sex chromosome number abnormality were 86%, 40%, 25% and 73%, respectively. Among those pregnant women receiving NIPT, the three proportions of pregnant women with advanced age, lowrisk but asking for NIPT and critical risk in their serum screening were 37.70%, 32.35% and 16.09%, respectively. The positive rate of NIPT in those women with abnormal ultrasonic soft index was statistically higher than that in others (P＜0.01). Conclusions：NIPT has high detection rates of trisomy 21, trisomy 13, trisomy 18 and sex chromosome abnormalities. It has high positive predictive values of trisomy 21 and sex chromosome number abnormality. NIPT has been widely accepted by those pregnant women with advanced age, low-risk but asking for NIPT and critical risk in serum screening. NIPT has a good clinical prospect because NIPT can greatly reduce the invasive prenatal diagnosis. [ABSTRACT FROM AUTHOR]