BACKGROUND: Currently, there have been studies on three-dimensional digitalization and visualization systems for adult acupoints, but there are not many reports on the visualization of pediatric acupoints based on real pediatric digital sectional anatomical datasets. OBJECTIVE: To design and develop a digital three-dimensional visualization system for children’s neck acupoints, to provide a basis for acupuncture and moxibustion, meridian and acupoint science teaching, clinical practice, acupuncture manipulation practice, and acupuncture safety research, and to provide a basis for the development of children’s acupoint simulation system. METHODS: Based on a real cross-sectional anatomical dataset of pre-school boys, a three-dimensional digital virtual anatomical model of the neck region of children and internal multi-organ three-dimensional reconstruction were completed using PhotoShop 2021 and Digihuman Reconstruction System software. A database of 11 acupoints was compiled, including Fengfu and Fengchi, using the Unity database language. A three-dimensional model of children’s neck anatomy, acupoint database, and writing acupuncture operation codes were integrated in Unity3D software. A three-dimensional digital visualization system for children’s neck acupoints was successfully created, which integrated simulation acupoint positioning, three-dimensional acupoint anatomy, acupuncture training, clinical teaching, and acupuncture safety research. RESULTS AND CONCLUSION: (1) This study was based on real child specimens. Manual layer by layer segmentation of cross-sectional images was used to ensure the accuracy of the three-dimensional model to the greatest extent possible. The 3D software Digihuman Reconstruction System was utilized to extract and save independent segmentation data. PhotoShop 2021 software was collaborated with to complete dozens of three-dimensional reconstruction anatomical models of the outer skin of the neck and its internal bone structure, cervical spinal cord, blood vessels and nerves, muscles, and ligaments in children. The basic morphology and overall contour integrity verification of each independent structure were completed in MeshLab software. The 3-material research 13.0 software was applied for final fine tuning and anatomical position confirmation, successfully simulating and restoring the true anatomical morphology of the neck of preschool children. (2) Based on and referring to the national standards of the People’s Republic of China, a database of commonly used acupoints in children’s neck region was collected and organized, including their names, meridians, positioning, local anatomy, needle insertion levels, acupuncture methods, acupuncture accidents and prevention, acupoint indications, and two-dimensional anatomical sectional images. (3) Unity3D software was employed to integrate the three-dimensional model of children’s neck, acupuncture simulation operation, and acupoint database, and a three-dimensional digital children’s neck acupoint acupuncture visualization system was successfully constructed. The system displayed information on children’s neck acupoints, two-dimensional and three-dimensional anatomical structures, and achieved two-dimensional and three-dimensional acupuncture simulation functions and acupuncture safety research functions for children’s neck acupoints. Based on the ultra-thin sectional anatomical dataset of real child specimens, the first three-dimensional digital and visualization system for acupoints in the neck region of children had been constructed. Compared with previous acupoint acupuncture systems, it is more in line with the anatomical and morphological development characteristics of Asian children and has high application value in the fields of acupuncture safety research, clinical teaching, and acupuncture simulation training. [ABSTRACT FROM AUTHOR]