Search

In order to establish the quality control method of Tian-Dong-Tang-Gan powder (TDTGP), TLC was used to qualitatively identify the total saponins of Panax notoginseng in TDTGP, the content of Panax notoginseng saponins in TDTGP was determined by high performance liquid chromatography, and the content of total polysaccharides in TDTGP was determined by the anthrone sulfate method. Iodine-potassium iodide reaction, ninhydrin reaction, Molish reaction, and ultraviolet spectroscopy are carried out to qualitatively identify polysaccharides in TDTGP. The results showed that TDTGP and Panax notoginseng control materials had the same spots in the same position in the TLC, and the spots were clear and non-interference, which could effectively identify the total saponins of Panax notoginseng. High performance liquid chromatography can effectively determine the content of total saponins of Panax notoginseng in TDTGP, which was stable and reliable. The content of total polysaccharides in TDTGP was 59.90% determined by anthrone sulfuric acid method. The results of physical and chemical properties showed that the reaction of iodine-potassium iodide and ninhydrin was negative, the Molish reaction was positive, and ultraviolet spectrum analysis showed no nucleic acid and protein. The experiment indicates that the method established in the experiment is simple and easy to implement, with high stability and reproducibility, and can effectively control the quality of TDTGP. [ABSTRACT FROM AUTHOR]