Background and Objectives: Light spectrum of growing environment is a determinant factor for plant growth and photosynthesis. Photoregulation is an effective strategy to improve productivity of plants. Light sources such as metal-halide, fluorescent, high-pressure sodium, neon lamps and light-emitting diode (LED) can be used for production of plants in closed environments instead of sun light. Nowadays, by using the LED technology, it is possible to study the physiological effect of different light spectra for optimization of growth conditions and for increase the production of plants in controlled environments. Due to high marketability, producers continually investigate to maximize yield and productivity of sage plant. The main purpose of this study was to examine and compare different combinations of LED light spectra on morphological, growth and biochemical response of sage plant. Materials and Methods: In this study, the effects of different light spectra were implemented and performed as a pot experiment soilless media in the plant growth chamber based on a completely randomized design with 6 lighting spectra including white, blue, red, red: blue (30:70, 50:50 and 70:30) with tree replication. The light intensity in all growth chambers was adjusted to photosynthetic photon flux density (PPFD) of 250 ±10 μmol m-2 s-1 intensity and light spectrum were monitored using a sekonic light meter. Growth condition was set at 14/10 h day/night cycles, 25/20 oC day/night temperatures and 40% relative humidity. In this study, different morphological traits (plant height, root length, root volume, stem diameter, number of leaves, Leaf length, Leaf width, Leaf area, specific leaf area, Number of nodes, Internode length, Number of branches), vegetative parameters, photosynthetic pigment concentrations and biochemical characteristics were measured analyzed. Data analysis of variance (ANOVA) was performed using IBM SAS software (Version 9.1), and the differences between means were assessed using Duncan’s multiple range tests at P≤0.01. Results: The results showed that the morphological, growth and biochemical characteristics of sage plant were affected by different light spectra. Red: blue combinational lights caused a better growth in comparison with monochromatic red and blue lights. The plants grown under red light had the tallest stem in comparison with the stem lengths of plants grown under other light spectra. The highest shoot and root fresh weight were measured in red: blue (70:30) light. Highest root dry weight and root volume were detected under red: blue (70:30) and lowest values of them were observed under red light. In the present study, increasing the ratio of blue light led to the generation of short and small plants, while increasing the ratio of red light led to the improvement of growth characteristics. Concentrations of all photosynthetic pigments in the sage leaves were significantly influenced by the light spectra. The highest Chl a and Chl b concentration was observed under red: blue (50:50) and red: blue (70:30) lights. Highest Chl a+b content was detected under red: blue (70:30). Highest amount of total phenolic content and antiradical activity was observed in leaves of plants grown under red: blue (70:30) light. Conclusion: Growth, morphology and biochemical characteristics of sage plants were considerably influenced by light spectra in this study. In conclusion, combined red and blue lights (70:30) by inducing of production of more photosynthetic pigments improved growth and improving growth of sage plant. Therefore, it can be expressed that the presence of both wavelengths (blue and red) is necessary for a better and more complete growth of the plant. In general, it can be suggested that the use of LEDs can result in better economic production-controlled environment systems. [ABSTRACT FROM AUTHOR]