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Agricultural reforms, land consolidation, and the abandonment of livestock farming effects grassland ecosystems worldwide. Utilising data from four medium-sized livestock farms across different regions in Lithuania, we assess floristic composition, productivity, and grass quality in both permanent grasslands and leys. Our findings reveal significant differences in flora diversity, with 120 species identified in permanent grasslands compared to only 20 in leys. Additionally, dry matter yield was notably higher in leys (13.97 t ha−1) than in permanent grasslands (5.66 t ha−1), underscoring the productivity potential of leys. The crude protein levels remained stable across both types, but leys demonstrated significantly lower neutral detergent fibre content, indicating better forage quality. However, the high biodiversity of permanent grasslands supports ecosystem services. [ABSTRACT FROM AUTHOR]