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Additional file 6: Phylogenetic tree of Haemonchus contortus and Ovis aries short-chain dehydrogenases (SDRs) in full details. A consensus phylogenetic tree was constructed using the Maximum Likelihood method based on the Whelan And Goldman model [27] in MEGA7. Initial tree is display, the bootstrap consensus tree was calculated (100 replicates), partitions are denoted above branches. Hco_SDRs analysed are marked by black dot, other Hco_SDRs are marked by black triangle.

Additional file 9: The comparison of expression levels of SDRs based on the relative quantification by qPCR (left axis, circles and dotted line) and relative quantification based on TPM values (transcript per million - right axes, squares and full line) all TPM data for SDRs available in Additional file 2) by differential RNA sequencing [39]).

Additional file 8: The comparison of all Hco_SDRs. Multiple alignment was performed using MultAlin [44]. Red residues represent high (>90%) consensus level and blue residues represent low (>50%) consensus level.

5 ABSTRACT Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Bc. Karolína Štěrbová Supervisor: prof. RNDr. Lenka Skálová Ph.D. Title of diploma thesis: Changes in expression of biotransformation enzymes in nematodes affected by albendazole Barber's pole worm (Haemonchus contortus) is highly pathogenic parasitic nematode, which assaults gastrointestinal system of small ruminants. Disease. Which caused is named by the agent haemonchoses. Long-term infection leads to develop of anaemia, lose weight of host and often even death. For treatment of this disease, synthetic antiparasitic drugs called anthelmintics are used. However, worldwide problem is growth of resistance against all used anthelmintics. Cause of this problem is probably common and unreasonable using of anthelmintics and the exposure of the parasites to sub-lethal doses. This thesis is focused on study of the mechanisms of resistance of barber's pole worm to albendazole, anthelmintic belonging to benzimidazoles group. The increased expression of biotransformation enzymes could contribute to resistance development. In our study, the influence of albendazole on expression of selected genes in adults of H. contortus from the ISE (Inbread Susceptible Edinburgh) strain was studied. Worms...

Additional file 1. The sequences, amplicon sizes, and efficiencies of primers.

Annotation: Bachelor's dissertation discuss about eating disorders, its caise and medical treatment. Dissertation is divided into two parts, theoretical and practical. In the first part, we will get to know a complex information about eating disorders issue. In the second one, we will find a research based on quality interview method. Interviews are processed with casuistry and grounded theory.