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23 results on '"Špaková, Ivana"'

4. The Pivotal Role of the Key Angiogenic Factors in the Development of Endometrioid Pathologies of the Uterus and Ovary.

Author

Sabolová, Gabriela, Špaková, Ivana, Artimovič, Peter, Bohuš, Peter, Rabajdová, Miroslava, and Mareková, Mária

Subjects
*VASCULAR endothelial growth factors, *ADENOCARCINOMA, *WOUND healing, *CELL communication, *GENOMICS, *DATA analysis, *RESEARCH funding, *OVARIAN tumors, *CELL motility, *DESCRIPTIVE statistics, *ENDOMETRIOSIS, *ENDOMETRIAL tumors, *CELL lines, *RNA, *ONE-way analysis of variance, *STATISTICS, *CONFIDENCE intervals, *DATA analysis software, *NEOVASCULARIZATION, *TRANSFORMING growth factors-beta
Abstract

Simple Summary: Angiogenesis is a synchronous mechanism of new blood vessel formation that is controlled by pro- and anti-angiogenic factors. Angiogenesis differs from tissue to tissue, especially in pathological conditions. The exact nature of the co-operation between angiogenic markers is still unknown. Herein, we describe the possible critical changes in VEGF-A, TGF-β1, ANG1, ANG2, and HIF-1α in ectopic endometriosis (with elevated pro-angiogenic factors in all studied fields), in ovarian endometrioid adenocarcinoma (showing typical upregulation on the TGF-β1 axis), and two different types of endometrial carcinoma (with an extreme pathological ANG2/ANG1 axis for mixed mesodermal tumors). Understanding the angiogenic mechanism within pathological tissues could help to improve angiogenic treatment strategies and lead to better prognoses. A characteristic feature of uterine pathologies is a specific change in cell metabolism, which predominantly manifests as a shift in the need for nutrients, thereby directing cells to engage in different angiogenic marker activities. Angiogenesis is one of the main signals supporting the survival and development of cells and tissues not only under physiological conditions. Therefore, it is necessary that we understand pathological hyperactivation in all uterine diseases, from endometriosis through ovarian endometrioid adenocarcinoma to malignant transformed cells of the uterine epithelium and body. This work presents the gene expression results of selected angiogenesis targets (VEGF-A, TGF-β1, ANG1/2, and HIF-1α), cell migration, and cell–cell interaction determined in vitro. Our results suggest that angiogenesis varies in the tested pathological conditions (ectopic endometriosis—12Z; ovarian endometrioid adenocarcinoma—A2780; tumors—SK-UT-1 and RL-95-2) compared to physiological angiogenesis (HME1). The differential expression of angiogenic factors may contribute (or is a contributing factor) to the observed differences to acknowledge an inherent variability in angiogenesis among cell lines. Determining the genomic phenomena responsible for processes associated with inadequate angiogenesis in the pelvic region could help us to develop individual treatment strategies and explain resistance to treatment. [ABSTRACT FROM AUTHOR]

Published
2024
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6. Fluorescence biomarkers of malignant melanoma detectable in urine

Author

Špaková Ivana, Dubayová Katarína, Nagyová Vladimíra, and Mareková Mária

Subjects
nad(p)h, lipofuscin, hif-1α, mitf-m, igf1, Chemistry, QD1-999
Abstract

Malignant melanoma (MM) is a cancerous transformation of melanocytes. It is a disease with the worst response to therapy and, compared to other malignancies, presents much earlier with metastases. MM still belongs to relatively late-detected malignant diseases. Even so, the MM mortality rate is up to 96% for a relatively small incidence (5%). The gold standard for MM diagnosis is a histopathological examination that requires invasive surgery. An invasive sampling method of a biological material can be a stressful factor for the patient, which is often the reason why patients do not seek medical assistance as soon as possible. Our goal was to find a link between metabolites in urine and the stage of MM. Two excitation peaks at 360–370 nm and 450 nm were characterised in spectra of urine samples. The emission spectra have shown one significant peak at 410–460 nm. After addition of glutathione reductase to the samples, fluorescence dropped down only in patient samples and hidden fluorophores appeared. Malignant diseases are associated with the presence of specific metabolites that can be detected fluorescently in biological material such as urine, which can be a suitable alternative for an early detection of cancer or for tracking changes during and after treatment.

Published
2020
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9. Oxidative Stress and the Nrf2/PPARγ Axis in the Endometrium: Insights into Female Fertility.

Author

Artimovič, Peter, Badovská, Zuzana, Toporcerová, Silvia, Špaková, Ivana, Smolko, Lukáš, Sabolová, Gabriela, Kriváková, Eva, and Rabajdová, Miroslava

Subjects
OXIDATIVE stress, CELL communication, NEOVASCULARIZATION, FERTILITY, PHYSIOLOGY
Abstract

Successful pregnancy depends on precise molecular regulation of uterine physiology, especially during the menstrual cycle. Deregulated oxidative stress (OS), often influenced by inflammatory changes but also by environmental factors, represents a constant threat to this delicate balance. Oxidative stress induces a reciprocally regulated nuclear factor erythroid 2-related factor 2/peroxisome proliferator-activated receptor-gamma (Nrf2/PPARγ) pathway. However, increased PPARγ activity appears to be a double-edged sword in endometrial physiology. Activated PPARγ attenuates inflammation and attenuates OS to restore redox homeostasis. However, it also interferes with physiological processes during the menstrual cycle, such as hormonal signaling and angiogenesis. This review provides an elucidation of the molecular mechanisms that support the interplay between PPARγ and OS. Additionally, it offers fresh perspectives on the Nrf2/PPARγ pathway concerning endometrial receptivity and its potential implications for infertility. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Serum trace element levels and activity of enzymes associated with oxidative stress in endometriosis and endometrial cancer.

Author

Rabajdová, Miroslava, Špaková, Ivana, Smolko, Lukáš, Abrahamovská, Michaela, Baranovičová, Barbora, Birková, Anna, Vašková, Janka, and Mareková, Mária

Subjects
ENDOMETRIAL cancer, ENDOMETRIOSIS, OXIDATIVE stress, GLUTATHIONE transferase, TRACE elements, INTRACELLULAR space, GLUTATHIONE reductase
Abstract

Endometriosis and endometrial cancer are closely related to oxidative stress. However, the direct relationship between copper and zinc levels and oxidative stress in the extracellular and intracellular space remains unclear. The presented study is focused on the determination of serum Zn and Cu levels, glutathione concentration and enzyme activity in three groups: patients diagnosed with endometrial cancer (EC), patients diagnosed with endometriosis (EM), and a healthy control group. Spectrophotometric determination of trace elements revealed that levels of zinc and copper were lower in blood plasma of patients with endometriosis as compared with the other groups; however, there were no significant differences in the Cu/Zn ratio. Furthermore, significantly increased blood serum glutathione levels were detected in both EM and EC groups compared with the control group. While the activity of superoxide dismutase (SOD) was similar across the studied groups, we observed differences in the activity of other enzymes associated with oxidative stress, including glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione S‐transferase (GST), between the control group and the EM and EC patients. Additionally, analysis of gene expression based on free circulating mRNA indicated significant differences in the expression of SOD isoenzymes between the patient groups and the control group; expression of GPx isoenzymes was also altered. Obtained results may have potential application in diagnostics as well as monitoring of endometriosis and endometrial cancer. [ABSTRACT FROM AUTHOR]

Published
2024
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13. Small Non-Coding RNAs as New Biomarkers to Evaluate the Quality of the Embryo in the IVF Process

Author

Toporcerová, Silvia, primary, Špaková, Ivana, additional, Šoltys, Katarína, additional, Klepcová, Zuzana, additional, Kľoc, Marek, additional, Bohošová, Júlia, additional, Trachtová, Karolína, additional, Peterová, Lucia, additional, Mičková, Helena, additional, Urdzík, Peter, additional, Mareková, Mária, additional, Slabý, Ondřej, additional, and Rabajdová, Miroslava, additional

Published
2022
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14. Investigation of novel Mn(II) fenamato complexes with neocuproine and their effects on endometrial cell lines.

Author

Klepcová, Zuzana, Špaková, Ivana, Madreiter-Sokolowski, Corina T., Graier, Wolfgang, Kalinová, Katarína, Samoová, Erika, Smolková, Romana, Smolko, Lukáš, and Rabajdová, Miroslava

Subjects
*CELL lines, *MOLECULAR structure, *LIGANDS (Chemistry), *BENZOIC acid, *ENDOMETRIAL cancer, *INFLAMMATION, *X-ray diffraction, *SCHIFF bases
Abstract

Two novel Mn(II) complexes with non-steroidal anti-inflammatory drugs, fenamic (Hfen = 2-(phenylamino)benzoic acid) and flufenamic acid (Hflu = N-(3-trifluoromethylphenyl)-2-aminobenzoic acid) and neocuproine (neo) as a supporting ligand were designed, synthesized and characterized. Their molecular structure was determined by the single-crystal X-ray diffraction method, which revealed that the complexes are isostructural and form analogous molecules [Mn(neo)(fen)2] and [Mn(neo)(flu)2]. Both complexes create an interesting structural motif resembling molecular tweezers, with an open cavity between the fenamato and flufenamato ligands, and exhibit moderate radical scavenging activity, with the fenamato complex being more active. The cytotoxic effects of the complexes were studied on three selected cell lines, including epithelial hTERT HME1 used as a healthy control, endometriotic 12Z and endometrial cancer SK-UT-1. The complexes were most active against the 12Z among the studied cell lines, while the flufenamato complex, which showed an IC50 value around 2 μM, was able to effectively trigger apoptosis through the caspase pathway. The impact of the complexes on the expression of selected genes in the tested cell lines was further investigated by qRT-PCR methods and their DNA binding properties towards the isolated genomic DNA samples were evaluated by competitive binding studies with ethidium bromide. Although some differences in the biological activity of the complexes were found, it can be concluded that both complexes target the inflammatory processes on a cellular level. [ABSTRACT FROM AUTHOR]

Published
2023
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17. List of contributors

Author

Ruchika Agrawal, Pallavi Bhat Ajakkala, Amjad Ali, Qinza Ali, Gautam Anand, Faryal Mehwish Awan, Hayeqa Shahwar Awan, Amina Basheer, Maloyjo Joyraj Bhattacharjee, Isabel S. Carvalho, James Chapman, Daniel Cozzolino, Vijaya Kumar Deekshit, Salah ud Din, Shailendra Dwivedi, Shruti Dwivedi, Supriya Gupta, Mubashir Hassan, Zhixia He, Shanmugam Hemaiswarya, Chia-Hsien Hsu, Aqsa Ikram, Špaková Ivana, Yusuf Izci, Sabariswaran Kandasamy, Šoltýs Katarína, Surekha Kishore, Ballamoole Krishna Kumar, Manish Kumar, Kenneth Lundstrom, Kľoc Marek, Mareková Mária, Rabajdová Miroslava, Radhieka Misra, Sanjeev Misra, Juliet Roshini Mohan Raj, Sawaira Naqvi, Mathiyazhagan Narayanan, Anam Naz, Urdzík Peter, Purvi Purohit, Praveen Rai, Rathinam Raja, Neelam Sangwan, Fatima Shahid, Saba Shahzadi, Nishat Ahmed Sheikh, Ammara Siddique, Aiman Tanveer, Tugba Tezcan, Basant K. Tiwary, Vi Khanh Truong, Abhimanyu Vasudeva, Maaz Waseem, Dinesh Yadav, Kanchan Yadav, Nisha Yadav, Pramod K. Yadav, Zainab Yaseen, and Tahreem Zaheer

Published
2022
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18. Contributors

Author

Shah Rukh Abbas, Richa Agarwal, Ruchika Agrawal, Saba Anjum, Jaspreet Singh Arora, Debmalya Barh, Siddhivinayak Barve, Muhammad Talha Basir, Attya Bhatti, Gülay Büyükköroğlu, Bilgen Çalışkan, Rekha Chawla, Mauricio Corredor, Pranjali Dhawal, Shailendra Dwivedi, Ota Fuchs, Edward Giniger, Naureen Ehsan Ilahi, Sana Ilahi, Muhammad Imran, Špaková Ivana, Zeenath Jehan, Peter John, Hari Shanker Joshi, Surekha Kishore, Birendra Kumar, Sudhir Shyam Kushwaha, Kenneth Lundstrom, Rabbiah Manzoor Malik, Sahar Malik, Rabajdová Mirka, Radhieka Misra, Sanjeev Misra, Mareková Mária, Amalia Muñoz-Gómez, Bela Nabar, Fakhira Nazir, Rasika Pawar, Abdur Rahman, Ayesha Rehman, Neelam S. Sangwan, Gençay Sevim, Anurag Sharma, Vipin Kumar Sharma, Vikas Shrivastava, Arvind Kumar Shukla, Abubakar Siddique, Toporcerová Silvia, Emine Şalva, Tahira Tayyaba, Behiye Şenel, Alka Tripathi, Abhimanyu Vasudeva, Nitin Wakchaure, Andrew P.K. Wodrich, and Vasudeo Zambare

Published
2022
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19. Detekcia expresie miRNA v IVF procese – možnosť využitia v diagnostike neplodnosti

Author

Kalinová, Katarína, Remešová, Dominika, Špaková, Ivana, Toporcerová, Silvia, and Rabajdová, Miroslava

Subjects
miREIA, RT-PCR, neplodnosť / infertility, miRNA
Abstract

SÚHRN Neplodnosť v súčasnosti postihuje približne 10% populácie a na základe vysokej prevalencie je zaraďovaná Svetovou zdravotníckou organizáciou medzi populačné ochorenia. Za fyziologických okolností sú v reprodukcii kľúčovými procesmi okno implantácie a receptivita endometria, ktoré podliehajú regulácii na génovej úrovni. Narušením tejto regulácie, môže následne dôjsť k patologickým zmenám, ktoré prispievajú k poruchám plodnosti. Hlavnými faktormi, ktoré ovplyvňujú reguláciu génovej expresie sú nekódujúce RNA, ktoré modifikujú génovú expresiu najmä na posttranskripčnej úrovni. Posttranskripčná úroveň regulácie sa považuje za kľúčovú pri vzniku rôznych deregulácií. Ich naakumulovaním môže dôjsť k vzniku rôznych patologických procesov. Objasnením mechanizmu pôsobenia konkrétnych nekódujúcich RNA v rámci infertility sa ukazuje ako možnosť ich využitia v diagnostike. Využitím detekcie citlivých miRNA markerov sa celý proces diagnostiky môže významne urýchliť a tiež prispieť k zlepšeniu výsledku liečby neplodnosti. ABSTRACT Infertility affects about 10% of the population currently and, due to its high prevalence, is classified by the World Health Organization as a population disease. Under physiological circumstances, the key processes in reproduction are implantation window and endometrial receptivity, which are regulated at the gene level. By disrupting this regulation, pathological changes can subsequently occur, which contribute to fertility disorders. The main factors that influence the regulation of gene expression are non-coding RNAs that modify gene expression, especially at the post-transcriptional level. The post-transcriptional level of regulation is considered to be key in the emergence of various dysregulations. Accumulation of this dysregulations can lead to various pathological processes. By elucidating the mechanism of action of specific non-coding RNAs in the context of infertility, is possible to use them in diagnostic process. By using the detection of sensitive miRNA markers, the whole diagnostic process can be significantly accelerated and also contribute to improving the outcome of infertility treatment., Práca vznikla na základe grantovej podpory: VEGA 1/0873/18 a VEGA 1/0620/19. Tieto projekty sú realizované v spolupráci s Gynekologicko-pôrodníckou klinikou UPJŠ LF a UNLP v Košiciach a Centrom pre asistovanú reprodukciu Gyncare v Košiciach., {"references":["BioVendor (2021a): miRNA-RT-qPCR. Dostupné na: https:// www.biovendor.com/mirna-rt-qpcr (Accessed: 7 March 2021).","BioVendor (2021b): Two-Tailed RT-qPCR. Dostupné na: https://www.biovendor.com/two-tailed-rt-qpcr (Accessed: 7 March 2021).","Buh Gašparič, M. et al. (2010): Comparison of nine different real-time PCR chemistries for qualitative and quantitative applications in GMO detection. 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(2009): The role of microRNAs in endometriosis and associated reproductive conditions. Hum Reprod Update, pp. 142–165. doi: 10.1093/humupd/dmp034.","Öner, Ç. (2019): Two different mechanisms of two different non-coding RNAs—MicroRNAs and PIWI-interacting RNAs: From origin to cancer. In AGO-Driven Non-Coding RNAs. Elsevier, pp. 3–34. doi: 10.1016/b978-0-12-815669-8. 00001-4.","Pan, Q. et al. (2007): The expression profile of micro-RNA in endometrium and endometriosis and the influence of ovarian steroids on their expression. Molecular Human Reproduction, 13(11), pp. 797–806. doi: 10.1093/molehr/gam063.","Pateisky, P. et al. (2018): hsa-miRNA-154-5p expression in plasma of endometriosis patients is a potential diagnostic marker for the disease. Reproductive BioMedicine Online, 37(4), pp. 449–466. doi: 10.1016/j.rbmo.2018.05.007.","Petracco, R. et al. (2011): MicroRNA 135 regulates HOXA10 expression in endometriosis. 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Published
2021
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20. Is There a Role for the IGF System and Epidermal Growth Factor (EGF) in the Pathogenesis of Adrenocortical Adenomas? A Preliminary Case-Control Study.

Author

LAZÚROVÁ, Ivica, JOCHMANOVÁ, Ivana, SOTAK, Štefan, ŠPAKOVÁ, Ivana, and MAREKOVÁ, Mária

Subjects
EPIDERMAL growth factor, METABOLIC syndrome, GROWTH factors, CASE-control method, INSULIN resistance, INTERLEUKIN-23, SOMATOTROPIN
Abstract

Adrenal incidentalomas (AI) are very common and mostly they are non-functioning adenomas (NFA). NFAs are often associated with insulin resistance and metabolic syndrome. Several biomarkers, including certain growth factors, may participate in the pathogenesis of metabolic changes in patients with adrenal adenomas. Patients with NFA and age-matched control subjects were enrolled in the study. Data on age, gender, presence of metabolic syndrome or its components were obtained for each subject. Blood samples were obtained and glycemia, insulinemia, lipid profile, and selected growth factor levels were measured. Forty-three patients with NFA and 40 controls were included in the study. Differences were not found in the metabolic syndrome and its components prevalence or in the biochemical profile between patients and the control group. Significant differences were noticed in the levels of IGF1, IGF2, and IGFBP3 (p=0.016, p=0.005, p=0.004, respectively), but there were no differences in VEGF or EGF concentrations. In NFA patients, an association between glycemia and EGF levels was present (p=0.026). No significant correlations between tumor size and insulin or growth factor concentrations were present in AI patients. Significantly higher serum IGF1, IGF2, and IGFBP3 concentrations in NFA patients may support the role of the IGF axis in the pathogenesis of adrenocortical lesions. No correlation between IGFs or IGFBP3 and parameters of glucose or lipid metabolism was found. Present results may support the role of the growth hormone axis rather than hyperinsulinemia and insulin resistance in the pathogenesis of adrenocortical adenomas. [ABSTRACT FROM AUTHOR]

Published
2020
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