26 results on '"Šimunić I"'
Search Results
2. Influence of Drainpipe Spacing on Nitrate Leaching and Maize Yield
- Author
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Simunic, I., Mesic, M., Sraka, M., Likso, T., and Coga, L.
- Published
- 2011
3. Comparison of three mathematical models for the estimation of 10-day drain discharge
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Pandžić, K., Šimunić, I., Tomić, F., Husnjak, S., Likso, T., and Petošić, D.
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- 2006
- Full Text
- View/download PDF
4. POGODNOST POLJOPRIVREDNOG ZEMLJIŠTA ZA NATAPANJE I MANJAK VODE U TLU PRI UZGOJU VAŽNIJIH POLJOPRIVREDNIH KULTURA NA PODRUČJU MEĐIMURSKE ŽUPANIJE.
- Author
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Husnjak, S., Šimunić, I., Kušan, V., Jungić, Danijela, and Magdić, I.
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FARMS ,AGRICULTURAL resources ,SOIL moisture ,ATMOSPHERIC temperature ,PRECIPITATION probabilities ,POTATOES ,AMARANTHUS palmeri - Abstract
Copyright of Agronomy Journal / Agronomski Glasnik (0002-1954) is the property of Croatian Society of Agronomists and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2022
5. Idejni projekt odvodnje i navodnjavanja objekta 'Đolta' - dionica
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Špoljar, Andrija, Kvaternjak, Ivka, and Šimunić, I.
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odvodnja ,navodnjavanje ,idejni projekt - Abstract
Temeljem provedenih terenskih i laboratorijskih istraživanja u tijeku 2015. godine te na osnovi postojećih podataka (Bašić et el., 1988 ; Vukadinović, 2012) izrađena je pedološka karta mjerila 1 : 5 000 s pripadajućom bazom pedoloških podataka. Također je izvršena procjena sadašnje i buduće namjenske pogodnosti tla za navodnjavanje i utvrđeni su prioriteti za navodnjavanje, uređenje i zaštitu tla. Uz navedeno, ova dionica agronomske osnove obuhvatila je i ocjenu kakvoće vode za navodnjavanje, date su preporuke za hidro i agromelioracijsko uređenje zemljišta, izračunati su osnovni elementi doziranja vode i utvrđena je redukcija prinosa uzgajanih kultura u uvjetima bez navodnjavanja.
- Published
- 2015
6. Impact of precipitation upon nitrate leaching and crop yield at different pipe drainage spacing
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Šimunić, I., Shutoska, Marija Vukelić, Mustać, I., Tanaskovik, V., Tatjana Prentovik, and Dimitrievski, Dragi
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education ,food and beverages ,Precipitation ,pipe spacing ,drainage discharge ,nitrate leaching ,crop yield ,Pipe spacing ,Drainage discharge ,Nitrate leaching ,Crop yield - Abstract
The goal of three-year investigations was to determine the influence of precipitation upon drainage discharge and its duration, concentration of nitrate in drainage water, amount of nitrates leached and yields of crops grown: winter wheat ; oilseed rape and soybea, at different pipe drainage spacings (15 m, 20 m, 25 m and 30 m) on pseudogley-gley soil. Investigation were carried out at the experimental amelioration site in the middle of Posavina, in the period from October 2008 up to September 2011. total nitrogen rates Applied amounted to 183.6 kg/ha (2008/09), 175.5 kg/ha (2009/10) and 130 kg/ha (2011). Results show that drainage discharge and its duration depended on the amount and distribution of precipitations during the study period. The highest drainage discharge was determined at the narrowest pipe spacing (15 m), while its longest duration was observed at the widest spacing (30 m. Drainage discharge was not registered in the growing season of the dry 2011. The higest nitrate concentration in drainage water, in all pipe spacing variants, was recorded with the occurrence of the First drainage discharges and was higher than MAC (10 mg/L NO3-N). It was only after the autumn crop harvest that nitrate concentration was below from MAC. The amount of nitrates leached, in each year and at each pipe spacing, depended on the drainage discharge and nitrate concentration in drainage water. Crop yield were statistically significant between the set pipe spacings in the 2008/09 and 2009/10 year, while was not statistically significant in 2011. Effect of pipe in drainage surplus water from soil was positive in humid years, while in the dry year was not registered effect of pipes.
- Published
- 2012
7. Water Quality in Hydroameliorated Agricultural Areas
- Author
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Šimunić, I., Orlović-Leko, P., Likso, T., Filipović, V., and Tatiana Minkina
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hydroameliorated soil ,drainage water quality ,nitrate ,chlortoluron ,Hidroameliorated soil ,Drainage water quality ,Nitrate ,Chlortoluron - Abstract
Three-year investigations (2007-2009) of water quality in hydroameliorated agricultural areas were carried out at the experimental amelioration field “Jelenščak” Kutina, on hydroameliorated Gleyic Podzoluvisol. Soil was drained in four different drainpipe spacing variants (15 m, 20 m, 25 m and 30 m), set up in four replications. The areas of spacing variants were: 1425 m2, 1900 m2, 2375 m2 and 2850 m2. The same crop was grown in each research year in all variants and the same agricultural management practices were applied. Winter wheat was grown in 2007 and in 2009 and soybean in 2008. Samples of drainage water were taken at drainpipe outlets into the canal. The following parameters were determined in the samples: nitrate concentration and concentration of chlortoluron. Based on the drainage water analysis, it was established that nitrate concentration as well as chlortoluron concentration exceeded the prescribed MAC values (10 mg.dm-3 NO3-N) in each year and in all variants. Nitrogen concentration in drainage water exceeded the MAC in five months (2006/07), in two months (2008) and in seven months (2008/09). Concentration of chlortoluron in drainage water exceeded the MAC (100 ŋg.dm-3) in five months (2006/07) and in seven months (2008/09). Maximum nitrate concentration was up to 28.42 mg.dm-3, and that of chlortoluron up to 365 ŋg.dm-3.
- Published
- 2011
8. Suvišna voda u dreniranim tlima
- Author
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Šimunić I., Tomić F.
- Subjects
suvišna voda - Abstract
In the period 1999-2001, investigations involving a drainpipe spacing of 15 m combined with contact hydraulic material – gravel were conducted on drained Gleyic Podzoluvisol with the following objectives: To determine the total annual drainage discharge (excess water) and compare it with the mathematical model after Palmer. Reference evapotranspiration was calculated according to the Penman-Monteith method. Investigations were carried out at the amelioration trial field Jelenščak near Kutina, on drained Gleyic Podzoluvisol. The studied drainpipe spacing was 15 m in combination with contact hydraulic material – gravel (ø ; 5-25 mm). Drainpipe length was 95 m, diameter 65 mm, average slope 3 ‰ and average depth 1 m. Drainpipes discharged directly into open canals. Plastic (PVC) pipes, corrugated and perforated, were used. Drainage discharge was measured continuously by means of automatic electronic meters (limnimeters), which were installed at the drainpipe end discharging into open canals. Maize was sown at the beginning of May and harvested in October 1999. Winter wheat was sown in the autumn of 2000 and harvested in July 2001. Excess water measured with electronic meters amounted to 236 mm in 1999, 130 mm in 2000, and 133 mm in 2001. Excess water in soil calculated by Palmer’ s method was 263 mm in 1999, 124 mm in 2000, and 160 mm in 2001. The obtained results clearly show that the difference between of direct measurements and those given by Palmer’ s method was the lesser in 2000 (drainage discharge was higher for 6 mm), while differences were indentical in 1999 and in 2001 (drainage discharges were higher for 27 mm). Consequently, excess water in drained soil of the central Sava valley calculated by Palmer’ s method is acceptable and gave rather reliable date of excess water in drained soil.
- Published
- 2004
9. Navodnjavanje korištenjem koeficijenta irigacije u uzgoju jabuke i lubenice
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Šimunić I., Tomić F.
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irigacioni koeficijent ,jabuka ,lubenica - Abstract
The goal of the investigations was to determine the onset of irrigation using the coefficient of irrigation, which is based on the calculation of everyday evapotranspiration. The coefficient of irrigation represents the water consumption (mm) per one degree of mean daily air temperature (oC ). Three-year investigations (1999-2001) were carried out at an apple plantation on the soil type Calcaric Fluvisol and water-melon plantation on the soil type Eutric Gleysol, hydromeliorated. Irrigation of apple was applied by means of mini sprinklers. Irrigation rate was calculated by the standard method and amounted to 47.6 mm. Irrigation of water-melon was applied by means of drip system and irrigation rate amounted to 15.6 mm, 31, 2 mm and 39, 0 mm. The coefficient of irrigation was estimated for decades periods according to the data on: soil moisture, precipitation, mean daily air temperature and the amount of water added with irrigation. The said data were processed to calculate the coefficient of irrigation, per decades, for the entire irrigation period. In 1999, the coefficient of irrigation for apple ranged from 0.17 mm/oC in the third decade of May to 0.40 mm/oC in the first and second decades of August. In 2000, the coefficient of irrigation ranged from 0.20 mm/oC in the first decade of May to 0.63 mm/oC in the third decade of August. In the last investigation year, the coefficient of irrigation amounted to 0.18 mm/oC in the first decade of May to 0.61 mm/oC in the second and third decades of August. In 1999, the coefficient of irrigation for water-melon ranged from 0.19 mm/oC in the second and third decades of May to 0.43 mm/oC in the third decade of June. In 2000 the coefficient of irrigation, ranged from 0.21 mm/oC in the second and third decades of May to 0.43 mm/oC in the third decade of June. In 2001, the coefficient of irrigation amounted to 0.25 mm/oC in the second decade of May to 0.47 mm/oC in the third decade of June. Water consumption for a particular day is calculated by multiplying the coefficient of irrigation by the mean daily air temperature (oC). When, in adding up the consumption of water per days, the previously added irrigation rate is spent, irrigation should be applied again. This fairly practical procedure of determining the onset of irrigation can be applied in a wider area with equal or similar pedological and climatic conditions.
- Published
- 2003
10. Doziranje vode prema koeficijentu irigacije u uzgoju lubenice
- Author
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Šimunić I., Tomić F., Romić D.
- Subjects
irigacioni koeficijent ,lubenica - Abstract
The goal of the investigations was to determine the onset of irrigation using the coefficient of irrigation, which is based on the calculation of everyday evapotranspiration. The coefficient of irrigation represents the water consumption (mm) per one degree of mean daily air temperature (oC). Three-years investigation (1999-2001) were carried out at a water-melon plantation in Neretva Vally, on the soil type Eutric Gleysol, hydromeliorated. Irrigation was applied by means of drip system. Irrigation rate was calculated by the standard method and amounted to 15.6 mm, 31, 2 mm and 39, 0 mm. The coefficient of irrigation was estimated for ten-day periods (decades) according to the data on: soil moisture, precipitation, mean daily air temperature and the amount of water added with irrigation. Soil moisture was estimated gravimetrically. When soil moisture approached the value of lentocapillary moisture (0.625 MPa), irrigation was applied with the amount of water corresponding to the irrigation rate. The said data were processed to calculate the coefficient of irrigation, per decades, for the entire irrigation period. The average coefficient of irrigation ranged from 0.22 mm/oC in the second and the third decade of May to 0.45 mm/oC in the third decade of June. Water consumption for a particular day is calculated by multiplying the coefficient of irrigation by the mean daily air temperature (oC). When, in adding up the consumption of water per days, the previously added irrigation rate is spent, irrigation should be applied again. This fairly practical procedure of determining the onset of irrigation can be applied in water-melon production also in a wider area with equal or similar pedological and climatic conditions.
- Published
- 2002
11. režim vlage tla melioriranog glejnog stagnisola
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Husnjak S., Bogunović M., Šimunić I.
- Subjects
drenirano tlo ,podzemna voda ,nedrenirano tlo - Abstract
The paper presents the results of investigation of performance of drainage systems on ameliorated gleyic stagnosol in the Central Sava Basin, in order to recommend pipe drainage for the purpose of more intensive use of drained land. Investigation of the soil moisture regime were carried out on drained and non-drained gleyic stagnosol in the period from 1987 to 1991, within regular crop production which included monitoring of soil moisture dynamics, ground water levels, occurrence of stagnant water and drain outflows. Subsoil pipe drains on the drained plot were placed at the depth of 0.9 m, with the spacing of 20 m. for the vegetation period (April-September) small differences have been determined between the durations of the wet phase in non-drained and drained gleyic stagnosol. In this period the average annual wet phase lasts 17 days at the depth of 15 cm, 47 days at 30 cm and 10 days at 60 cm in non-drained soil, and 23 days at 15 cm, 43 days at 30 cm, and 10 days at 60 cm in drained soil. In the non-vegetation period (October-march), the differences in duration of the wet phase between drained and non- drained soil were also small at depths of 15 and 60 cm, while the difference at 30 cm was considerably larger The average annual duration of the wet phase was 50 days at 15 cm, 140 days at 30 cm, and 17 days at 60 cm in non-drained soil, or 50 days at 15 cm, 97 days at 30 cm, and 13 days at 60 cm in drained soil. Statistically significant difference in the dynamics of soil moisture between drained and non-drained soil was determined only for the depth of 30 cm, and only in the non-vegetation period. The ground water level was practically always below 1.0 m ; however in the upper part of the soil profile the presence of stagnatrain water has been determined. No significant difference between the duration of presence of stagnat water in drained and non- drained soil has been determined. Maximum values of drain discharge, which were about 0.003 m/day were over three times less than the design drainage module The noticed differences between crop yields on drained and non- drained soils are not significant. Research of the soil moisture regime in soil and monitoring of ground and stagnant water in drained and non-drained gleyic stagnosol show that, in drained soils the problem of evacuation of excess water in rhyzospheres still remains, first of all as a consequence of the absence of agricultural measures, i.e. subsoiling, liming and humization. In such conditions, drainage systems have only limited effects.
- Published
- 2002
12. Comparison of three mathematical models for the estimation of 10-day drain discharge
- Author
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Pandžić, K., primary, Šimunić, I., additional, Tomić, F., additional, Husnjak, S., additional, Likso, T., additional, and Petošić, D., additional
- Published
- 2005
- Full Text
- View/download PDF
13. The content of Pb, Zn and Cd in hydroameliorated soil and drainage water and their uptake by plants
- Author
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Šimunić, I., primary, Tomić, F., additional, Kisić, I., additional, and Romić, M., additional
- Published
- 2002
- Full Text
- View/download PDF
14. Content of mineral N in soil and tomato yields considering fertigation and mulch
- Author
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Jungić, D., Jerko Gunjača, Herak-Ćustić, M., Šimunić, I., Ban, D., and Sraka, M.
- Subjects
fertigation ,mulch ,soil ,mineral nitrogen, tomato - Abstract
The objective of this study was to determine the effect of application of different amounts of N fertilizer added by fertigation and different mulches on the total mineral N in the plough soil layer and yield of tomatoes. The two-year study (2007 and 2009) was conducted on hydromeliorated deep Terra Rossa near Pula. In two-factor experiment, set in a split-plot design, the main factor was N fertilization (NF) in three levels: 60 (NF1), 120 (NF2), and 180 (NF3) kg N ha-1. Subfactor was soil mulch (M), also in three levels: without mulch (WM), straw mulch (SM), and mulch with black polyethylene film (BPM). Different NF significantly affected the amount of N min in the soil. Only in 2009, mulch as well as interaction between NF and DAT had significant impact on the amount of N min in soil. Nitrogen fertilization did not significantly affect the total yields of tomatoes, except the application of NF2 and BPM that generated significantly higher yields of tomatoes in 2007. Recommended technology is application of NF2 and BPM, but with ecological point of view it would be justified to use the nitrogen fertilization with only 60 kg N ha-1.
15. Impact of water shortage in the soil on crop yields,Utjecaj nedostatka vodeutlu na prinose poljoprivrednih kultura
- Author
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Šimunić, I., Spalević, V., Vukelić-Shutoska, M., Šoštarić, J., and Monika Marković
16. Determination of Total Phenolic Content of Royal Jelly by Using a Spectrophotometric Method.
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Šimunić, I., Kristić, D., Ibragić, S., Čulum, D., Klepo, L., and Tahirović, I.
- Subjects
- *
PHENOL content of food , *ROYAL jelly , *SPECTROPHOTOMETRY - Abstract
The aim of this study was to determine the total phenolic content (TPC) of royal jelly harvested in the municipality of Konjic, by a spectrophotometric method using the Folin-Ciocalteu reagent. A gallic acid (GA) solution was used as a standard. All measurements were performed in triplicate at the wavelength of 764 nm The TPC was expressed in mg of GA equivalent (GAE) per 100 g royal jelly sample. The mean value of TPC in 12 samples of royal jelly was 411.72±8.83 mg GAE/100 g. The mean TPC in the 11 polyfloral samples of royal jelly (407.87±9.46 mg GAE/100 g) was slightly higher than mean TPC (369.53±11.65 mg GAE/100 g) in the monofloral sample (only one sample, measured in triplicate). The selected and applied spectrophotometric method proved to be an efficient and simple method for the quantitative determination of TPC in samples of royal jelly. [ABSTRACT FROM AUTHOR]
- Published
- 2018
17. Determination of Total Protein Content in Royal Jelly Samples by an UV Spectrophotometric Method.
- Author
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Kristić, D., Šimunić, I., Ibragić, S., Čulum, D., Klepo, L., and Tahirović, I.
- Subjects
- *
PROTEIN content of food , *ROYAL jelly , *SERUM albumin - Abstract
The aim of this study was to quantify the total protein content (TPrC) in samples of royal jelly harvested in the municipality of Konjic, using an UV spectrophotometric method. Bovine serum albumine was used as a protein standard to conctruct the calibration curve. The value of TPrC in polyfloral samples of royal jelly was 21.21±1.18%, while monofloral samples of royal jelly (Acacia flowers) showed the TPrC of 20.23±0.24%. Compared to literature data, this method produced higher values of TPrC in the complex matrices such as royal jelly. The employed spectrophotometric method proved to be very simple and convenient for the quantification of total proteins. [ABSTRACT FROM AUTHOR]
- Published
- 2018
18. Usporedba jezičnih sposobnosti i obiteljske jezične okoline osnovnoškolske djece sa i bez slušnog oštećenja
- Author
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Vidović, Jerka, Brković, Irma, and Z. Penezić, A. Slišković, V. Ćubela Adorić, Lj. Gregov, M. Nikolić, M. Nekić, J. Ombla, A. Šimunić i A. Tokić
- Subjects
jezične sposobnosti, obiteljska jezična okolina, slušno oštećenje, fonološko dekodiranje, stavovi o čitanju - Abstract
Cilj istraživanja bio je ispitati i usporediti jezične sposobnosti i obilježja obiteljske jezične okoline osnovnoškolske djece sa i bez slušnog oštećenja. Sudjelovalo je 209 djece od 3. do 8. razreda osnovne škole (10 - ero djece sa i 199 - ero bez slušnog oštećenja). Korišteni su zadaci fonološkog i ortografskog dekodiranja, Test nizova riječi, Test razumijevanja čitanja te Upitnik obite ljske jezične okoline. Prvi problem bio je ispitati primjenljivost tako konstruiranih zadataka jezičnih sposobnosti na gluhoj djeci. Univarijatne karakteristike zadataka pokazale su da su svi zadaci, osim ortografskog dekodiranja, bili teži gluhoj djeci. Drugi problem bio je ispitati razlike u uratku u zadacima između skupina djece koje su testirane usporedbom njihovih distribucija. Rezultati pokazuju da se gluhi u svim jezičnim testovima, osim ortografskog dekodiranja, nalaze ispod prosjeka čujućih, kao i po broju knjiga u kući s obzirom na obiteljsku jezičnu okolinu. Problem je bio i ispitati doprinos mjera jezičnih sposobnosti i mjera obiteljske jezične okoline razumijevanju čitanja. Hijerarhijska regresijska analiza za čujuće sudionike pokazala je kako zadaci i testovi jezičnih sposobnosti objašnjavaju značajan dio varijace razumijevanja čitanja, a od obiteljske jezične okoline, stavovi o čitanju imaju značajan samostalan doprinos. S obzirom na manji broj sudionika, na uzorku djece sa slušnim oštećenjem provedena je korelacijska analiza. Pronađeno je da su jezične sposobnosti povezane s razumijevanjem čitanja, s tim da je ortografsko dekodiranje jednako važno kao i fonološko (za razliku od čujuće djece). Broj članova obitelji povezan je s čitalačkim nav ikama i stavovima o čitanju, a zajedničke čitalačke aktivnosti s roditeljima i vrijeme provedeno s njima s prosjekom ocjena, po čemu se također razlikuju od čujuće djece. Ovi nalazi poklapaju se u većini slučajeva s nalazima iz literature te pokazuju u koj im područjima jezičnog razvoja gluhi pokazuju slabiji uspjeh od čujućih, ali i prostor na kojem se može raditi u svrhu poboljšanja njihovih jezičnih vještina.
- Published
- 2016
19. Što vaš kućni ljubimac govori o vašoj ličnosti?
- Author
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Gavranović, Petra, Vukasović Hlupić, Tena, and Z. Penezić, A. Slišković, V. Ćubela Adorić, Lj. Gregov, M. Nikolić, M. Nekić, J. Ombla, A. Šimunić i A. Tokić
- Subjects
ličnost ,Pet-faktorski model ,ljubitelji pasa ,ljubitelji mačaka - Abstract
Stereotipi o ljubiteljima pasa i ljubiteljima mačaka te razlike među njima često se spominju u svakodnevnom životu i prikazuju u medijima, a izazivaju i interes u području psihologije ličnosti. Dosadašnja istraživanja ne daju jednoznačne i konzistentne rezultate o prirodi i smjeru tih razlika. Cilj ovog istraživanja bio je ispitati odnos između osobina Pet-faktorskog modela ličnosti i preferencije određene vrste životinja. U tu svrhu provedeno je internetsko istraživanje u kojem je korištena elektronička verzija upitnika BFI, prikupljeni su sociodemografski podaci sudionika i postavljeno je pitanje o tome izjašnjava li se osoba kao ljubitelj pasa, ljubitelj mačaka, oboje ili nijedno od toga. Prikupljeni su podaci za 437 sudionika (315 žena) te se 49.4% sudionika izjasnilo kao ljubitelj pasa, 13.5% kao ljubitelj mačaka, 25.2% kao oboje i 11.9% kao nijedno od toga. Rezultati su pokazali da postoje statistički značajne razlike između ljubitelja pasa i ljubitelja mačaka na osobinama ekstraverzije i otvorenosti u smjeru da ljubitelji pasa postižu više rezultate na objema osobinama. Na poduzorku muškaraca te su razlike utvrđene na osobinama ekstraverzije, otvorenosti i savjesnosti u smjeru da ljubitelji pasa postižu više rezultate. Razlike između ljubitelja pasa i ljubitelja mačaka nisu potvrđene na poduzorku žena. Buduća istraživanja trebala bi se usmjeriti i na druge osobine ličnosti te ispitivanje razlika među ljudima koji preferiraju druge vrste životinja.
- Published
- 2016
20. Transplantation of neural stem cells improves recovery of stroke-affected mice and induces cell-specific changes in GSDMD and MLKL expression.
- Author
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Lisjak D, Alić I, Šimunić I, and Mitrečić D
- Abstract
Introduction: Stroke, the second leading cause of death and disability in Europe, is primarily caused by interrupted blood supply, leading to ischemia-reperfusion (IR) injury and subsequent neuronal death. Current treatment options are limited, highlighting the need for novel therapies. Neural stem cells (NSCs) have shown promise in treating various neurological disorders, including stroke. However, the underlying mechanisms of NSC-mediated recovery remain unclear., Methods: Eighty C57Bl/6-Tyrc-Brd mice underwent ischemic stroke induction and were divided into four groups: sham, stroke-affected, stroke-affected with basal cell medium injection, and stroke-affected with NSCs transplantation. NSCs, isolated from mouse embryos, were stereotaxically transplanted into the stroke-affected brains. Magnetic resonance imaging (MRI) and neurological scoring were used to assess recovery. Immunohistochemical analysis and gene expression assays were performed to evaluate pyroptosis and necroptosis markers., Results: NSC transplantation significantly improved neurological recovery compared to control groups. In addition, although not statistically significant, NSCs reduced stroke volume. Immunohistochemical analysis revealed upregulation of Gasdermin D (GSDMD) expression post-stroke, predominantly in microglia and astrocytes. However, NSC transplantation led to a reduction in GSDMD signal intensity in astrocytes, suggesting an effect of NSCs on GSDMD activity. Furthermore, NSCs downregulated Mixed Lineage Kinase Domain-Like Protein ( Mlkl ) expression, indicating a reduction in necroptosis. Immunohistochemistry demonstrated decreased phosphorylated MLKL (pMLKL) signal intensity in neurons while stayed the same in astrocytes following NSC transplantation, along with increased distribution in microglia., Discussion: NSC transplantation holds therapeutic potential in stroke recovery by targeting pyroptosis and necroptosis pathways. These findings shed light on the mechanisms underlying NSC-mediated neuroprotection and support their further exploration as a promising therapy for stroke patients., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Lisjak, Alić, Šimunić and Mitrečić.)
- Published
- 2024
- Full Text
- View/download PDF
21. The temporal dynamic of bradykinin type 2 receptor effects reveals its neuroprotective role in the chronic phase of cerebral and retinal ischemic injury.
- Author
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Justić H, Barić A, Ratko M, Šimunić I, Radmilović M, Pongrac M, Škokić S, and Dobrivojević Radmilović M
- Abstract
The activation of the bradykinin type 2 receptor is intricately involved in acute post-ischemic inflammatory responses. However, its precise role in different stages of ischemic injury, especially in the chronic phase, remains unclear. Following simultaneous cerebral and retinal ischemia, bradykinin type 2 receptor knockout mice and their controls were longitudinally monitored for 35 days via magnetic resonance imaging, fundus photography, fluorescein angiography, behavioral assessments, vascular permeability measurements, and immunohistochemistry, as well as glycemic status assessments. Without impacting the lesion size, bradykinin type 2 receptor deficiency reduced acute cerebral vascular permeability preventing the loss of pericytes and tight junctions. In the chronic phase of ischemia, however, it resulted in increased astrogliosis and cortical neuronal loss, as well as higher functional deficits. The retinal findings demonstrated a similar pattern. Bradykinin type 2 receptor deficiency delayed, but exacerbated the development of retinal necrosis, increased subacute vascular permeability, and promoted retinal ganglion cell loss in the chronic phase of ischemia. This investigation sheds light on the temporal dynamic of bradykinin type 2 receptor effects in ischemia, pointing to a therapeutic potential in the subacute and chronic phases of ischemic injury., Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
- Published
- 2024
- Full Text
- View/download PDF
22. Author Correction: Lusca: FIJI (ImageJ) based tool for automated morphological analysis of cellular and subcellular structures.
- Author
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Šimunić I, Jagečić D, Isaković J, Dobrivojević Radmilović M, and Mitrečić D
- Published
- 2024
- Full Text
- View/download PDF
23. Lusca: FIJI (ImageJ) based tool for automated morphological analysis of cellular and subcellular structures.
- Author
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Šimunić I, Jagečić D, Isaković J, Dobrivojević Radmilović M, and Mitrečić D
- Subjects
- Humans, Mitochondria, Image Processing, Computer-Assisted methods, Neurons
- Abstract
The human body consists of diverse subcellular, cellular and supracellular structures. Neurons possess varying-sized projections that interact with different cellular structures leading to the development of highly complex morphologies. Aiming to enhance image analysis of complex biological forms including neurons using available FIJI (ImageJ) plugins, Lusca, an advanced open-source tool, was developed. Lusca utilizes machine learning for image segmentation with intensity and size thresholds. It performs particle analysis to ascertain parameters such as area/volume, quantity, and intensity, in addition to skeletonization for determining length, branching, and width. Moreover, in conjunction with colocalization measurements, it provides an extensive set of 29 morphometric parameters for both 2D and 3D analysis. This is a significant enhancement compared to other scripts that offer only 5-15 parameters. Consequently, it ensures quicker and more precise quantification by effectively eliminating noise and discerning subtle details. With three times larger execution speed, fewer false positive and negative results, and the capacity to measure various parameters, Lusca surpasses other existing open-source solutions. Its implementation of machine learning-based segmentation facilitates versatile applications for different cell types and biological structures, including mitochondria, fibres, and vessels. Lusca's automated and precise measurement capability makes it an ideal choice for diverse biological image analyses., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
24. The Oxygen and Glucose Deprivation of Immature Cells of the Nervous System Exerts Distinct Effects on Mitochondria, Mitophagy, and Autophagy, Depending on the Cells' Differentiation Stage.
- Author
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Jagečić D, Petrović DJ, Šimunić I, Isaković J, and Mitrečić D
- Abstract
Perinatal brain damage, one of the most common causes of lifelong impairment, is predominantly caused by a lack of oxygen and glucose during early development. These conditions, in turn, affect cells of the nervous tissue through various stages of their maturation. To quantify the influence of these factors on cell differentiation and mitochondrial parameters, we exposed neural cell precursors to oxygen and glucose deprivation (OGD) during three stages of their differentiation: day 1, day 7, and day 14 (D1, D7, and D14, respectively). The obtained results show that OGD slows down cellular differentiation and causes cell death. Regardless of the level of cell maturity, the overall area of the mitochondria, their length, and the branching of their filaments decreased uniformly when exposed to OGD-related stress. Moreover, the cells in all stages of differentiation exhibited an increase in ROS production, hyperpolarization of the mitochondrial membrane, and autophagy. Interestingly, day 7 was the only stage in which a significant increase in mitochondrial fission, along with measurable instances of mitophagy, were detected. Taken together, the results of this study suggest that, apart from common reactions to a sudden lack of oxygen and glucose, cells in specific stages of neural differentiation can also exhibit increased preferences for mitochondrial fission and mitophagy. Such findings could play a role in guiding the future development of novel therapeutic approaches targeting perinatal brain damage during specific stages of nervous system development.
- Published
- 2023
- Full Text
- View/download PDF
25. Redefining the Koizumi model of mouse cerebral ischemia: A comparative longitudinal study of cerebral and retinal ischemia in the Koizumi and Longa middle cerebral artery occlusion models.
- Author
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Justić H, Barić A, Šimunić I, Radmilović M, Ister R, Škokić S, and Dobrivojević Radmilović M
- Subjects
- Animals, Disease Models, Animal, Infarction, Middle Cerebral Artery pathology, Ischemia pathology, Longitudinal Studies, Mice, Middle Cerebral Artery, Brain Ischemia diagnostic imaging, Brain Ischemia etiology, Brain Ischemia pathology, Retinal Diseases diagnostic imaging, Retinal Diseases etiology
- Abstract
Cerebral and retinal ischemia share similar pathogenesis and epidemiology, each carrying both acute and prolonged risk of the other and often co-occurring. The most used preclinical stroke models, the Koizumi and Longa middle cerebral artery occlusion (MCAO) methods, have reported retinal damage with great variability, leaving the disruption of retinal blood supply via MCAO poorly investigated, even providing conflicting assumptions on the origin of the ophthalmic artery in rodents. The aim of our study was to use longitudinal in vivo magnetic resonance assessment of cerebral and retinal vascular perfusion after the ischemic injury to clarify whether and how the Koizumi and Longa methods induce retinal ischemia and how they differ in terms of cerebral and retinal lesion evolution. We provided anatomical evidence of the origin of the ophthalmic artery in mice from the pterygopalatine artery. Following the Koizumi surgery, retinal responses to ischemia overlapped with those in the brain, resulting in permanent damage. In contrast, the Longa method produced only extensive cerebral lesions, with greater tissue loss than in the Koizumi method. Additionally, our data suggests the Koizumi method should be redefined as a model of ischemia with chronic hypoperfusion rather than of ischemia and reperfusion.
- Published
- 2022
- Full Text
- View/download PDF
26. Overview of Neural Tube Defects: Gene-Environment Interactions, Preventative Approaches and Future Perspectives.
- Author
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Isaković J, Šimunić I, Jagečić D, Hribljan V, and Mitrečić D
- Abstract
Neural tube defects (NTDs) are the second most common congenital malformations of humans, characterized by impaired development of the central nervous system. Even though the etiology of most birth defects remains undetermined, genetic and environmental risk factors in the background of NTDs have been identified and extensively reported. On top of genetic and nutritional risks which include mutations in both coding and non-coding regions and maternal folate status, respectively, recent years have seen a rise in the identification of a variety of teratogens that could be implicated in NTD development. These include polycyclic aromatic hydrocarbons, arsenic, pesticides, maternal hyperthermia and antibiotics as well as pain and seizure medication. With an increase in understanding of teratogens leading to NTD formation, preventative and treatment approaches have witnessed great advances throughout the years. While the most common preventative approach includes folic acid food fortification as well as suggested inositol supplementation, treatment and management approaches differ greatly depending on the developmental stage and the site of the lesion and include prenatal surgery, stem cell transplantation and postnatal surgery. Because NTDs still represent a large health and financial burden for the patient and society as a whole, it is crucial to investigate potential risk factors and develop novel approaches in order to fully prevent this category of disorders.
- Published
- 2022
- Full Text
- View/download PDF
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