1. Recombinant production of Thermus aquaticus single-strand binding protein for usage as PCR enhancer
- Author
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İsa Gökçe, Rizvan Imamoğlu, İskender Şahingöz, and Özlem Kaplan
- Subjects
Engineering, Chemical ,Thermus aquaticus ,biology ,Chemistry ,Engineering, Multidisciplinary ,General Medicine ,Mühendislik, Ortak Disiplinler ,biology.organism_classification ,eye diseases ,Mühendislik, Kimya ,law.invention ,Single-stranded binding protein ,stomatognathic diseases ,Biochemistry ,stomatognathic system ,law ,Polymerase Chain Reaction,Single Strand Binding Protein,Thermus aquaticus ,Recombinant DNA ,biology.protein ,bacteria ,Pharmacology (medical) ,Enhancer ,skin and connective tissue diseases ,Polymerase chain reaction - Abstract
Single-stranded DNA-binding (SSB) proteins play an important role in DNA metabolism involving DNA replication, recombination, and repair in all living beings. In molecular biology, SSB proteins are used as enhancers to increase the efficiency and specificity of PCR. Thermostable SSB protein eliminates secondary structure or dimer formation and significantly increase the effectiveness of amplification of DNA fragments. In this study, it was ensured that the SSB gene of thermophilic bacteria Thermus aquaticus (T. aquaticus) was cloned into the pET28b vector and expressed in E. coli BL21 (DE3) PLysE cells. Then, the purification of the SSB protein produced in E. coli BL21 (DE3) PLysE cells was performed. 20 mg SSB protein was obtained from 1L bacterial culture, and its purity was more than 90%. It was shown by the PCR experiment that the SSB protein produced in this study could increase the amplification efficiency.
- Published
- 2020