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Reasons for performing study Mesenchymal stem cells ( MSCs) have been used to treat equine tendonitis with promising results; however, little is known about the potential migration of these cells. Objectives To assess the possible migration of MSCs from an implantation site in the superficial digital flexor tendon ( SDFT) to a lesion in the SDFT of the contralateral limb. Study design In vivo experimental study. Methods Adipose-derived MSCs were isolated from 4 healthy horses. Lesions were induced in the SDFTs of both forelimbs, followed by intralesional implantation of autologous adipose-derived MSCs labelled with nanocrystals into one of the limbs. Flow cytometry of the peripheral blood mononuclear cells and fluorescence microscopy of biopsies of the SDFT lesions were used to search for the labelled cells. Results Labelled cells were detected among the peripheral blood mononuclear cells in all animals, but labelled cells were present only in the SDFTs that were treated with the intralesional implants. Conclusion Nanocrystals were a valuable in vivo marker of MSCs to be used for tendonitis treatment. Although migration of MSCs to the bloodstream was observed, it was not possible to identify the labelled cells in the untreated tendons. [ABSTRACT FROM AUTHOR]

The article discusses research on the effectiveness of stem cell therapy in the treatment of tendinitis and osteoarthritis in equine medicine. It aims to characterize the adipose-derived mesenchymal stem cells in horse. It looks at collagenase digestion as the basis of the process of isolating the nucleated cell fraction from the adipose tissue. A proposed standard protocol to ensure the correct characterization of equine mesenchymal stem cells is also discussed.