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305 results on '"Álvarez, Claudio A."'

1. Extending an Intelligent Tutoring System for Oral Communication with Peer Assessment Capabilities: An Evaluation Study

Author

Ibarra, Javier, Álvarez, Claudio, Recabarren, Matías, Goos, Gerhard, Series Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Santos, Patricia, editor, Álvarez, Claudio, editor, Hernández-Leo, Davinia, editor, Kobayashi, Minoru, editor, and Zurita, Gustavo, editor

Published
2024
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2. Exploring Group Behavior and Discussion Productivity in Anonymous Chatrooms for Ethical Decision-Making

Author

Álvarez, Claudio, Zurita, Gustavo, Farías, Antonio, Yunga, Manuel, Goos, Gerhard, Series Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Santos, Patricia, editor, Álvarez, Claudio, editor, Hernández-Leo, Davinia, editor, Kobayashi, Minoru, editor, and Zurita, Gustavo, editor

Published
2024
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3. Academic Assessment: Usability Evaluation of an Integrated Platform for Students with Disabilities

Author

Rojas, Luis A., Calderon, Juan Felipe, Castro, John W., Álvarez, Claudio, Goos, Gerhard, Series Editor, Hartmanis, Juris, Founding Editor, van Leeuwen, Jan, Series Editor, Hutchison, David, Editorial Board Member, Kanade, Takeo, Editorial Board Member, Kittler, Josef, Editorial Board Member, Kleinberg, Jon M., Editorial Board Member, Kobsa, Alfred, Series Editor, Mattern, Friedemann, Editorial Board Member, Mitchell, John C., Editorial Board Member, Naor, Moni, Editorial Board Member, Nierstrasz, Oscar, Series Editor, Pandu Rangan, C., Editorial Board Member, Sudan, Madhu, Series Editor, Terzopoulos, Demetri, Editorial Board Member, Tygar, Doug, Editorial Board Member, Weikum, Gerhard, Series Editor, Vardi, Moshe Y, Series Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Woeginger, Gerhard, Editorial Board Member, Coman, Adela, editor, and Vasilache, Simona, editor

Published
2024
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4. Improving the Learning Experience in Ethics Education with Groupware: A Case Study

Author

Álvarez, Claudio, Zurita, Gustavo, Rojas, Luis A., Goos, Gerhard, Series Editor, Hartmanis, Juris, Founding Editor, van Leeuwen, Jan, Series Editor, Hutchison, David, Editorial Board Member, Kanade, Takeo, Editorial Board Member, Kittler, Josef, Editorial Board Member, Kleinberg, Jon M., Editorial Board Member, Kobsa, Alfred, Series Editor, Mattern, Friedemann, Editorial Board Member, Mitchell, John C., Editorial Board Member, Naor, Moni, Editorial Board Member, Nierstrasz, Oscar, Series Editor, Pandu Rangan, C., Editorial Board Member, Sudan, Madhu, Series Editor, Terzopoulos, Demetri, Editorial Board Member, Tygar, Doug, Editorial Board Member, Weikum, Gerhard, Series Editor, Vardi, Moshe Y, Series Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Woeginger, Gerhard, Editorial Board Member, Coman, Adela, editor, and Vasilache, Simona, editor

Published
2024
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6. Analyzing Peer Influence in Ethical Judgment: Collaborative Ranking in a Case-Based Scenario

Author

Álvarez, Claudio, Zurita, Gustavo, Carvallo, Andrés, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Takada, Hideyuki, editor, Marutschke, D. Moritz, editor, Alvarez, Claudio, editor, Inoue, Tomoo, editor, Hayashi, Yugo, editor, and Hernandez-Leo, Davinia, editor

Published
2023
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8. Hydroclimate variability in the Tropical Andes recorded by δ18O isotopes from a new network of Polylepis tarapacana tree-rings

Author

Álvarez, Claudio, Christie, Duncan A., González-Reyes, Álvaro, Veblen, Thomas T., Helle, Gerhard, LeQuesne, Carlos, Rodriguez-Caton, Milagros, Szejner, Paul, Flores-Sáez, Felipe, Gipoulou-Zúñiga, Tania, Suazo-Álvarez, Manuel, Muñoz-Salazar, Tomás, Aliste, Diego, Morales, Mariano S., Muñoz, Ariel, and Villalba, Ricardo

Published
2024
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10. Comparison of Different Pedagogical Designs for an ITS: The Case of Oral Speech as an Ill-Defined Domain

Author

Recabarren, Matías, Correa, Vicente, Álvarez, Claudio, Milrad, Marcelo, Kacprzyk, Janusz, Series Editor, Gomide, Fernando, Advisory Editor, Kaynak, Okyay, Advisory Editor, Liu, Derong, Advisory Editor, Pedrycz, Witold, Advisory Editor, Polycarpou, Marios M., Advisory Editor, Rudas, Imre J., Advisory Editor, Wang, Jun, Advisory Editor, Milrad, Marcelo, editor, Otero, Nuno, editor, Sánchez‑Gómez, María Cruz, editor, Mena, Juan José, editor, Durães, Dalila, editor, Sciarrone, Filippo, editor, Alvarez-Gómez, Claudio, editor, Rodrigues, Manuel, editor, Vittorini, Pierpaolo, editor, Gennari, Rosella, editor, Di Mascio, Tania, editor, Temperini, Marco, editor, and De la Prieta, Fernando, editor

Published
2023
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11. Development and Usability Evaluation of an Application for Language Literacy of Spanish-Speaking Children with Autism Spectrum Disorder

Author

Rojas, Luis A., Sorbello, Katrina, Castro, John W., Alvarez, Claudio, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Coman, Adela, editor, and Vasilache, Simona, editor

Published
2023
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12. Towards Cybersecure Maritime Supply Chains in Latin America and the Caribbean

Author

Alvarez, Claudio, Hinojosa, Camila, Gonzalez, Sebastián, Rojas, Luis, Clausen, Uwe, Series Editor, Hompel, Michael ten, Series Editor, de Souza, Robert, Series Editor, García Alcaraz, Jorge Luis, editor, Manotas Duque, Diego Fernando, editor, González-Ramírez, Rosa Guadalupe, editor, Chong Chong, Mario Gustavo, editor, and de Brito Junior, Irineu, editor

Published
2023
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13. Scaffolding of Intuitionist Ethical Reasoning with Groupware: Do Students’ Stances Change in Different Countries?

Author

Álvarez, Claudio, Zurita, Gustavo, Farías, Antonio, Collazos, César, González-Calleros, Juan Manuel, Yunga, Manuel, Pezoa, Álvaro, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Wong, Lung-Hsiang, editor, Hayashi, Yugo, editor, Collazos, Cesar A., editor, Alvarez, Claudio, editor, Zurita, Gustavo, editor, and Baloian, Nelson, editor

Published
2022
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20. Detection and Localization of IL-8 and CXCR1 in Rainbow Trout Larvae in Response to Pseudomonas aeruginosa Lipopolysaccharide.

Author

Santana, Paula A., Forero, Juan C., Guzmán, Fanny, Gaete, Sandra, Acosta, Félix, Mercado, Luis A., and Álvarez, Claudio A.

Subjects
MUCOUS membranes, RAINBOW trout, INTERLEUKIN receptors, CELL receptors, FISH pathogens
Abstract

Simple Summary: The salmonid industry is susceptible to opportunistic pathogens particularly affecting fish in early developmental stages. Understanding the immunological capacity during these stages is crucial for effective disease control. The receptor for Interleukin 8 (CXCR1), plays a key role in various inflammatory conditions. This study analyzed the expression of IL-8R and IL8 in rainbow trout larvae in response to bacterial lipopolysaccharide (LPS). The larvae 19 day post-hatching (dph) exhibited strong immune responses after 8 h of LPS stimulation, with IL-8 and omCXCR1 protein expression increasing. The use of specific antisera allowed localization of both proteins in mucosal tissue cells. This study highlights the effectiveness of LPS immersion in activating the innate immune system of trout larvae using IL-8/CXCR1 as immunological markers. The salmonid industry faces challenges due to the susceptibility of fish to opportunistic pathogens, particularly in early developmental stages. Understanding the immunological capacity during these stages is crucial for developing effective disease control strategies. IL-8R, a member of the G-protein-coupled receptor family, acts as a receptor for Interleukin 8 (IL-8). The binding of IL-8 to IL-8R plays a major role in the pathophysiology of a wide spectrum of inflammatory conditions. This study focused on the immune response capacity of rainbow trout (Oncorhynchus mykiss) larvae by analyzing IL-8/CXCR1 response to lipopolysaccharide (LPS) from Pseudomonas aeruginosa. Previous research demonstrated that LPS from P. aeruginosa acts as a potent immunostimulant in teleost, enhancing pro-inflammatory cytokines. The methodology included in silico analysis and the synthesis and characterization of an omCXCR1-derived epitope peptide, which was used to produce omCXCR1-specific anti98 serum in mice. The research revealed that rainbow trout larvae 19 days post-hatching (dph) exhibited pronounced immune responses post-stimulation with 1 µg/mL of LPS. This was evidenced by the upregulated protein expression of IL-8 and omCXCR1 in trout larvae 2 and 8 h after LPS challenge, as analyzed by ELISA and immunohistochemistry. Furthermore, fluorescence microscopy successfully revealed the colocalization of IL-8 and its receptor in cells from mucosal tissues after LPS challenge in larvae 19 dph. These findings underscore the efficacy of LPS immersion as a method to activate the innate immune system in trout larvae. Furthermore, we propose IL-8 and its receptor as molecular markers for evaluating immunostimulation in the early developmental stages of salmonids. [ABSTRACT FROM AUTHOR]

Published
2024
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21. Automatic Content Analysis of Student Moral Discourse in a Collaborative Learning Activity

Author

Alvarez, Claudio, Zurita, Gustavo, Carvallo, Andrés, Ramírez, Pablo, Bravo, Eugenio, Baloian, Nelson, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Woeginger, Gerhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Hernández-Leo, Davinia, editor, Hishiyama, Reiko, editor, Zurita, Gustavo, editor, Weyers, Benjamin, editor, Nolte, Alexander, editor, and Ogata, Hiroaki, editor

Published
2021
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25. Six hundred years of South American tree rings reveal an increase in severe hydroclimatic events since mid-20th century

Author

Morales, Mariano S., Cook, Edward R., Barichivich, Jonathan, Christie, Duncan A., Villalba, Ricardo, LeQuesne, Carlos, Srur, Ana M., Ferrero, M. Eugenia, González-Reyes, Álvaro, Couvreux, Fleur, Matskovsky, Vladimir, Aravena, Juan C., Lara, Antonio, Mundo, Ignacio A., Rojas, Facundo, Prieto, María R., Smerdon, Jason E., Bianchi, Lucas O., Masiokas, Mariano H., Urrutia-Jalabert, Rocio, Rodriguez-Catón, Milagros, Muñoz, Ariel A., Rojas-Badilla, Moises, Alvarez, Claudio, Lopez, Lidio, Luckman, Brian H., Lister, David, Harris, Ian, Jones, Philip D., Williams, A. Park, Velazquez, Gonzalo, Aliste, Diego, Aguilera-Betti, Isabella, Marcotti, Eugenia, Flores, Felipe, Muñoz, Tomás, Cuq, Emilio, and Boninsegna, José A.

Published
2020

29. Evaluation of the Biological Activities of Peptides from Epidermal Mucus of Marine Fish Species from Chilean Aquaculture.

Author

Álvarez, Claudio A., Toro-Araneda, Teresa, Cumillaf, Juan Pablo, Vega, Belinda, Tapia, María José, Roman, Tanya, Cárdenas, Constanza, Córdova-Alarcón, Valentina, Jara-Gutiérrez, Carlos, Santana, Paula A., and Guzmán, Fanny

Abstract

The skin of fish is a physicochemical barrier that is characterized by being formed by cells that secrete molecules responsible for the first defense against pathogenic organisms. In this study, the biological activity of peptides from mucus of Seriola lalandi and Seriolella violacea were identified and characterized. To this purpose, peptide extraction was carried out from epidermal mucus samples of juveniles of both species, using chromatographic strategies for purification. Then, the peptide extracts were characterized to obtain the amino acid sequence by mass spectrometry. Using bioinformatics tools for predicting antimicrobial and antioxidant activity, 12 peptides were selected that were chemically produced by simultaneous synthesis using the Fmoc-Tbu strategy. The results revealed that the synthetic peptides presented a random coil or extended secondary structure. The analysis of antimicrobial activity allowed it to be discriminated that four peptides, named by their synthesis code 5065, 5069, 5070, and 5076, had the ability to inhibit the growth of Vibrio anguillarum and affected the copepodite stage of C. rogercresseyi. On the other hand, peptides 5066, 5067, 5070, and 5077 had the highest antioxidant capacity. Finally, peptides 5067, 5069, 5070, and 5076 were the most effective for inducing respiratory burst in fish leukocytes. The analysis of association between composition and biological function revealed that the antimicrobial activity depended on the presence of basic and aromatic amino acids, while the presence of cysteine residues increased the antioxidant activity of the peptides. Additionally, it was observed that those peptides that presented the highest antimicrobial capacity were those that also stimulated respiratory burst in leukocytes. This is the first work that demonstrates the presence of functional peptides in the epidermal mucus of Chilean marine fish, which provide different biological properties when the fish face opportunistic pathogens. [ABSTRACT FROM AUTHOR]

Published
2024
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30. A CSCL Script for Supporting Moral Reasoning in the Ethics Classroom

Author

Alvarez, Claudio, Zurita, Gustavo, Baloian, Nelson, Jerez, Oscar, Peñafiel, Sergio, Goos, Gerhard, Founding Editor, Hartmanis, Juris, Founding Editor, Bertino, Elisa, Editorial Board Member, Gao, Wen, Editorial Board Member, Steffen, Bernhard, Editorial Board Member, Woeginger, Gerhard, Editorial Board Member, Yung, Moti, Editorial Board Member, Nakanishi, Hideyuki, editor, Egi, Hironori, editor, Chounta, Irene-Angelica, editor, Takada, Hideyuki, editor, Ichimura, Satoshi, editor, and Hoppe, Ulrich, editor

Published
2019
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36. Promoting Active Learning in Large Classrooms: Going Beyond the Clicker

Author

Álvarez, Claudio, Baloian, Nelson, Zurita, Gustavo, Guarini, Fabio, Hutchison, David, Series editor, Kanade, Takeo, Series editor, Kittler, Josef, Series editor, Kleinberg, Jon M., Series editor, Mattern, Friedemann, Series editor, Mitchell, John C., Series editor, Naor, Moni, Series editor, Pandu Rangan, C., Series editor, Steffen, Bernhard, Series editor, Terzopoulos, Demetri, Series editor, Tygar, Doug, Series editor, Weikum, Gerhard, Series editor, Gutwin, Carl, editor, Ochoa, Sergio F., editor, Vassileva, Julita, editor, and Inoue, Tomoo, editor

Published
2017
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40. CollPhoto: A Paper + Smartphone Problem Solving Environment for Science and Engineering Lectures

Author

Alvarez, Claudio, Milrad, Marcelo, Borie, Francisco, Luna, Martín, Junqueira Barbosa, Simone Diniz, Series editor, Chen, Phoebe, Series editor, Cuzzocrea, Alfredo, Series editor, Du, Xiaoyong, Series editor, Filipe, Joaquim, Series editor, Kara, Orhun, Series editor, Kotenko, Igor, Series editor, Sivalingam, Krishna M., Series editor, Ślęzak, Dominik, Series editor, Washio, Takashi, Series editor, Yang, Xiaokang, Series editor, Yuizono, Takaya, editor, Zurita, Gustavo, editor, Baloian, Nelson, editor, Inoue, Tomoo, editor, and Ogata, Hiroaki, editor

Published
2014
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42. Polycystic ovary syndrome throughout a woman’s life

Author

Bellver, José, Rodríguez-Tabernero, Luis, Robles, Ana, Muñoz, Elkin, Martínez, Francisca, Landeras, José, García-Velasco, Juan, Fontes, Juan, Álvarez, Mónica, Álvarez, Claudio, Acevedo, Belén, and Group of interest in Reproductive Endocrinology (GIER) of the Spanish Fertility Society (SEF)

Published
2017
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