1. Primary cilia in chondrogenic differentiation of equine bone marrow mesenchymal stem cells: ultrastructural study
- Author
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Antonio Romero, B. Ranera, Ana Rosa Remacha, Inmaculada Martín, María José Luesma, C. Junquera, Clementina Rodellar, Irene Cantarero, Tomás Castiella, [Jose Luesma, Maria] Fac Med, Dept Human Anat & Histol, Zaragoza, Spain, [Junquera, Concepcion] Fac Med, Dept Human Anat & Histol, Zaragoza, Spain, [Jose Luesma, Maria] Aragon Hlth Sci Inst, Zaragoza, Spain, [Ranera, Beatriz] Aragon Hlth Sci Inst, Zaragoza, Spain, [Rosa Remacha, Ana] Aragon Hlth Sci Inst, Zaragoza, Spain, [Castiella, Tomas] Aragon Hlth Sci Inst, Zaragoza, Spain, [Romero, Antonio] Aragon Hlth Sci Inst, Zaragoza, Spain, [Martin, Inmaculada] Aragon Hlth Sci Inst, Zaragoza, Spain, [Rodellar, Clementina] Aragon Hlth Sci Inst, Zaragoza, Spain, [Junquera, Concepcion] Aragon Hlth Sci Inst, Zaragoza, Spain, [Cantarero, Irene] Fac Med, Physiol & Immunol, Dept Cellular Biol, Cordoba, Spain, [Cantarero, Irene] Maimonides Inst Biomed Res Cordoba IMIBIC, Cordoba, Spain, [Ranera, Beatriz] Fac Vet, Lab Biochem Genet Lagenbio, Zaragoza, Spain, [Rosa Remacha, Ana] Fac Vet, Lab Biochem Genet Lagenbio, Zaragoza, Spain, [Romero, Antonio] Fac Vet, Lab Biochem Genet Lagenbio, Zaragoza, Spain, [Martin, Inmaculada] Fac Vet, Lab Biochem Genet Lagenbio, Zaragoza, Spain, [Rodellar, Clementina] Fac Vet, Lab Biochem Genet Lagenbio, Zaragoza, Spain, [Castiella, Tomas] Lozano Blesa Univ Clin Hosp, Dept Pathol, Zaragoza, Spain, and University of Zaragoza, Spain
- Subjects
0301 basic medicine ,Morphology ,Pathology ,medicine.medical_specialty ,040301 veterinary sciences ,Progenitors ,Biology ,Matrix (biology) ,0403 veterinary science ,Extracellular matrix ,Primary cilium chondrocyte ,03 medical and health sciences ,Chondrocytes ,Osteoarthritis ,Receptors ,medicine ,Stromal cells ,Stem cell transplantation for articular cartilage repair ,Equine ,Mesenchymal stem cell ,04 agricultural and veterinary sciences ,Chondrogenesis ,030104 developmental biology ,medicine.anatomical_structure ,Blood ,Intraflagellar transport ,Ultrastructure ,Chondrocyte ultrastructure ,Equine BM-MSCs ,Muscle ,Bone marrow ,Stem cell ,Human adipose-tissue - Abstract
Locomotor system disorders in equine species, such as tendon lesions, osteoarthritis, or ligament injuries, are some of the most frequent causes of dramatic reduction in horse performance. Traditional therapies are aimed at the inflammatory process and pain, but they do not regenerate normal tendon or ligament matrix and do not reduce re-injured rates. Mesenchymal stem cells have started to use as therapeutic option to repair these injured tissues. Most studies have focused on their isolation, in vitro culture and pheno-typing. However, mesenchymal stem cell ultrastructure has been disregarded in the last years. We investigate the ultrastructural characteristics of these cells once differentiated into chondrocytes. Ultrastructural analysis was conducted on suspension cultures of differentiated chondrocytes from bone marrow mesenchymal stem cells by means of transmission electron microscopy. The morphologic characteristics of these cells, their ability to produce the extracellular matrix, and the presence of a single cilium could be indicative of the mesenchymal cells differentiation into chondrocyte phenotype. This study provides essential data to evaluate the degree of suitable phenotypic stability, for these cells can be used with repair purposes. (C) 2016 Elsevier Inc. All rights reserved.
- Published
- 2016