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72 results on '"[ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology"'

1. Response of life-history traits to artificial and natural selection for virulence and nonvirulence in a Drosophila parastitoid, Asobara tabida

Author: Moiroux , Joffrey, van Baaren , Joan, POYET , Mathilde, Couty , Aude, Eslin , Patrice, Prévost , Geneviève, Seguin , Jérémy, Le Roux , Vincent, Ecologie et Dynamique des Systèmes Anthropisés - UMR CNRS 7058 (EDYSAN), Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Institut méditerranéen de biodiversité et d'écologie marine et continentale (IMBE), Avignon Université (AU)-Aix Marseille Université (AMU)-Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique (CNRS), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Génétique et évolution des interactions hôtes-parasites, Département génétique, interactions et évolution des génomes [LBBE] (GINSENG), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Agence Nationale de la Recherche, Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Ecologie et Dynamique des Systèmes Anthropisés ( EDYSAN ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Picardie Jules Verne ( UPJV ), Institut méditerranéen de biodiversité et d'écologie marine et continentale ( IMBE ), Université d'Avignon et des Pays de Vaucluse ( UAPV ) -Aix Marseille Université ( AMU ) -Institut de recherche pour le développement [IRD] : UMR237-Centre National de la Recherche Scientifique ( CNRS ), Ecosystèmes, biodiversité, évolution [Rennes] ( ECOBIO ), Université de Rennes 1 ( UR1 ), and Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -INEE-Observatoire des Sciences de l'Univers de Rennes ( OSUR ) -Centre National de la Recherche Scientifique ( CNRS )
Subjects: Male, [ SDV.MP.PAR ] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Virulence, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Climate, Reproduction, Wasps, geographic variation, host resistance, Host-Parasite Interactions, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Drosophila melanogaster, trade-offs, [ SDV.EE.IEO ] Life Sciences [q-bio]/Ecology, environment/Symbiosis, Animals, encapsulation, Female, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Selection, Genetic, Life History Traits, Locomotion, Selective Breeding, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract: International audience; Co-evolution of host-parasitoid interactions is determined by the costs of host resistance, which received empirical evidence, and the costs of parasitoid virulence, which have been mostly hypothesized. Asobara tabida is a parasitoid, which mainly parasitizes Drosophila melanogaster and D. subobscura, the first species being able to resist to the parasitoid development while the second species is not. To parasitize resistant hosts, including D. melanogaster, A. tabida develops sticky eggs, which prevent encapsulation, but this virulence mechanism may be costly. Interindividual and interpopulation variation in the proportion of sticky eggs respectively allowed us to (i) artificially select and compare life-history traits of a virulent and a nonvirulent laboratory strain, and (ii) compare a virulent and a nonvirulent field strain, to investigate the hypothetical costs of virulence. We observed strong differences between the 2 laboratory strains. The nonvirulent strain invested fewer resources in reproduction and walked less than the virulent one but lived longer. Concerning the field strains, we observed that the nonvirulent strain had larger wings while the virulent one walked more and faster. All together, our results suggest that virulence may not always be costly, but rather that different life histories associated with different levels of virulence may coexist at both intra- and interpopulation levels.
Published: 2018
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2. The regulation of interspecific variations of shell shape in bivalves: an illustration with the common 'clams' fauna along French coasts

Author: Béguinot , Jean, Biogéosciences [Dijon] ( BGS ), and Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS )
Subjects: morphospace, [ SDV.BID ] Life Sciences [q-bio]/Biodiversity, selective pressure, Veneroida, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, covariance, shell contour, Mollusc, growth parameters, functional parameters
Abstract: 12 pages; International audience; I report an unexpected negative covariance occurring between two major parameters governing shell growth in marine bivalves, especially within the order Veneroida. This relationship is highlighted, here, considering a set of forty, rather common species of clams collected from French coasts. Interestingly, this negative covariance has two (geometrically related) consequences on the pattern of variation of shell shape at the inter-specific level: (i) An extended range of variation of shell elongation 'E' is made compatible with. (ii) A severely restricted range of variation of the ventral convexity 'K' of the shell contour. I suggest that: (i) The extended range of interspecific variation of the shell elongation 'E' results from a trend towards larger differentiation between species according to this functionally important parameter E, while, in contrast, (ii) The strongly restricted range of variation of the ventral convexity 'K' of the shell contour might arguably result from a common need for improved shell resistance, face to mechanical solicitations from the environment, either biotic or abiotic. Accordingly, the negative covariance reported between these two growth parameters is understood as the indirect consequence of the selective pressures applying primarily upon the functionally-relevant shell shape parameters E and K.
Published: 2018
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3. Unexpected covariances between shell growth parameters are controlling the range of interspecific variations of shell shape in the deep-sea clams genus Calyptogena (Bivalvia: Veneroida)

Author: Jean Béguinot, Biogéosciences [Dijon] ( BGS ), and Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS )
Subjects: [ SDV.BID ] Life Sciences [q-bio]/Biodiversity, History, Mollusk, Veneroida, biology, Range (biology), ecological constraint selective pressure, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Shell (structure), Interspecific competition, growth parameters, biology.organism_classification, Bivalvia, Deep sea, functional parameters, Computer Science Applications, Education, morphospace, Oceanography, Genus, shell contour, Geology
Abstract: 13 pages; International audience; In Bivalves, shell shape descriptors (in particular, elongation E, dissymmetry D, ventral convexity K) are considered functionally-relevant parameters, each of them likely being exposed to specific selective pressures, according to environmental conditions. Indeed, previous investigations, have repeatedly confirmed that different types of constraints do apply to the respective ranges of interspecific variations of E, D, K: in particular, a trend for a substantially extended range of interspecific variation of shell-elongation E (likely attributed to niche differentiation) and, on the contrary, a trend for a severely restricted range of interspecific variation of ventral-convexity K (closely centered at an optimum for mechanical resistance of valves). In turn, due to rigid geometrical relationships linking shell-shape to shell-growth, this pattern of constraints on shell-shape variations implies, mathematically, the occurrence of specifically associated covariances between shell-growth parameters, the occurrence of which were, indeed, repeatedly observed within all groups of shallow-waters clams examined so far. Yet, the fairly different environmental conditions prevailing at bathyal / abyssal depths invite addressing comparatively some typically deep-sea clams; hereafter the genus Calyptogena. Contrary to expectation, the same types of covariances between shell-growth parameters are reported in deep-sea Calyptogena as those reported in shallow-waters clams, thus suggesting less dissimilar patterns of constraints than expected between shallow waters and deep-sea conditions.
Published: 2018
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4. The effect of landscape complexity and microclimate on the thermal tolerance of a pest insect

Author: Alford, Lucy, Tougeron, Kévin, Pierre, Jean‐Sébastien, Burel, Francoise, Baaren, Joan van, Ecosystèmes, biodiversité, évolution [Rennes] ( ECOBIO ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -INEE-Observatoire des Sciences de l'Univers de Rennes ( OSUR ) -Centre National de la Recherche Scientifique ( CNRS ), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), FP7-PEOPLE-2012-IEF-326943, Seventh Framework Programme, Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)
Subjects: Nymph, Thermotolerance, QL, landscape ecology, agroecosystems, S1, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, biological control, Microclimate, cold tolerance, Environment, Cold Temperature, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, cereal aphids, climate change, Aphids, Animals, France, Seasons
Abstract: International audience; Landscape changes are known to exacerbate the impacts of climate change. As such, understanding the combined effect of climate and landscape on agroecosystems is vital if we are to maintain the function of agroecosystems. This study aimed to elucidate the effects of agricultural landscape complexity on the microclimate and thermal tolerance of an aphid pest to better understand how landscape and climate may interact to affect the thermal tolerance of pest species within the context of global climate change. Meteorological data were measured at the landscape level, and cereal aphids (Sitobion avenae, Metopolophium dirhodum and Rhopalosiphum padi) sampled, from contrasting landscapes (simple and complex) in winter 2013/2014 and spring 2014 in cereal fields of Brittany, France. Aphids were returned to the laboratory and the effect of landscape of origin on aphid cold tolerance (as determined by CTmin) was investigated. Results revealed that local landscape complexity significantly affected microclimate, with simple homogenous landscapes being on average warmer, but with greater temperature variation. Landscape complexity was shown to impact aphid cold tolerance, with aphids from complex landscapes being more cold tolerant than those from simple landscapes in both winter and spring, but with differences among species. This study highlights that future changes to land use could have implications for the thermal tolerance and adaptability of insects. Furthermore, not all insect species respond in a similar way to microhabitat and microclimate, which could disrupt important predator–prey relationships and the ecosystem service they provide.
Published: 2018
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5. Intraspecific maternal competition induces summer diapause in insect parasitoids

Author: Tougeron , Kévin, Hraoui , George, Le Lann , Cécile, Van Baaren , Joan, Brodeur , Jacques, Ecosystèmes, biodiversité, évolution [Rennes] ( ECOBIO ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -INEE-Observatoire des Sciences de l'Univers de Rennes ( OSUR ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Recherches en Biologie Végétale ( IRBV ), Université de Montréal [Montréal], Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherches en Biologie Végétale [Montréal] (IRBV), Université de Montréal (UdeM), Biological Control, Canada Research Chairs, ARED, French Région Bretagne, Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)
Subjects: host density, [ SDV.MP.PAR ] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Hot Temperature, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, intraspecific competition, fungi, Mothers, Diapause, Insect, aestivation, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Aphids, Animals, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Female, Seasons, bet-hedging, superparasitism
Abstract: International audience; Organisms often live in unpredictable environments and have to adopt life history strategies that optimize their fitness under these conditions. According to bet‐hedging theory, individuals can reduce variation in fitness outcomes by investing in different strategies at the same time. For arthropods, facultative summer diapause enables survival during dry and hot periods of the year, and can be triggered by a decrease in resource abundance. However, the effect of resource depletion on diapause induction has never been disentangled from the effect of the perception of the presence of competitors. Using two solitary parasitoid species of cereal aphids as a model system, Aphidius avenae (Haliday) and Aphidius rhopalosiphi (De Stefani‐Perez) (Hymenoptera: Braconidae), we tested whether (i) low absolute host density and/or (ii) high levels of parasitoid females’ competition lead to maternal‐induced summer diapause in parasitoid offspring. Under summer‐like climatic conditions, emerging parasitoid females were (i) reared alone and exposed to different host densities (from 5 to 130 aphids), or (ii) reared together with competing females (from 2 to 20 females) and then exposed individually to 50 aphids. For both parasitoid species, low aphid densities did not induce summer diapause. However, the incidence of summer diapause increased up to a maximum of 11% with increasing levels of competition experienced by female parasitoids. More than 60% of the females produced both diapausing and nondiapausing offspring after being kept at the two highest competition densities. Such a “spreading‐the‐risk” strategy has likely evolved to optimize parasitoid fitness by preventing the following generation from exposure to low populations of suitable hosts and high mortality from superparasitism. These results provide the first experimental evidence of direct maternal competition‐induced diapause in insects, and may change the way we apprehend the evolution of arthropod seasonal ecology, by considering intraspecific competition.
Published: 2018
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6. Carryover effects of larval exposure to different environmental bacteria drive adult trait variation in a mosquito vector

Author: Dickson, Laura, Jiolle, Davy, Minard, Guillaume, Moltini-Conclois, Isabelle, Volant, Stevenn, Ghozlane, Amine, Bouchier, Christiane, Ayala, Diego, Paupy, Christophe, Valiente Moro, Claire, Lambrechts, Louis, Interactions Virus-Insectes (IVI), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Centre international de recherches médicales de Franceville (CIRMF), Organisation Mondiale de la Santé (OMS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), University of Helsinki, Centre de Bioinformatique, Biostatistique et Biologie Intégrative (C3BI), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Biomics, Centre de Recherche et Innovation Technologique (CITECH), Institut Pasteur [Paris]-Institut Pasteur [Paris], This study was supported by the French government’s Investissement d’Avenir program, Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID), the French Agence Nationale de la Recherche (MOSQUIBIOTA project, grant ANR-16-CE35-0004), the City of Paris Emergence(s) program in Biomedical Research, and the French Centre National de la Recherche Scientifique, Ecology of Health (ECOSAN) program (grant AFRICAEDES). The Genomics Facility is a member of the 'France Génomique' consortium (grant ANR-10-INBS-09-08). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript., ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases'(2010), ANR-16-CE35-0004,MOSQUIBIOTA,Contribution de la diversité bactérienne intestinale à la capacité vectorielle d'Aedes aegypti(2016), ANR-10-INBS-09-01/10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), Centre International de Recherches Médicales de Franceville (CIRMF), Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-10-INBS-0009,France-Génomique,Organisation et montée en puissance d'une Infrastructure Nationale de Génomique(2010), Interactions Virus-Insectes ( IVI ), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle ( MIVEGEC ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ), Centre international de recherches médicales de Franceville ( CIRMF ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), University of Helsinki [Helsinki], Centre de Bioinformatique, Biostatistique et Biologie Intégrative ( C3BI ), Institut Pasteur [Paris], Centre d’Innovation et Recherche Technologique ( CITECH ), ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases' ( 2010 ), ANR-16-CE35-0004,MOSQUIBIOTA,Contribution de la diversité bactérienne intestinale à la capacité vectorielle d'Aedes aegypti ( 2016 ), ANR : France Génomique,ANR10-INBS-09-08, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Institut Pasteur [Paris] (IP)-Institut Pasteur [Paris] (IP), Biosciences, Centre of Excellence in Metapopulation Research, Life-history Evolution Research Group, and Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)
Subjects: moustique, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, système immunitaire, Mosquito Vectors, Breeding, Environment, DENGUE VIRUS-INFECTION, Microbial Ecology, Dengue, Quantitative Trait, Heritable, ALTERS SUSCEPTIBILITY, parasitic diseases, Antibiosis, Environmental Microbiology, [ SDV.EE.IEO ] Life Sciences [q-bio]/Ecology, environment/Symbiosis, Animals, Humans, développement larvaire, TEMPERATURE, Ecosystem, Research Articles, bactérie, Life Cycle Stages, Bacteria, Microbiota, GUT MICROBIOTA, fungi, 1184 Genetics, developmental biology, physiology, SciAdv r-articles, Life Sciences, ANOPHELES-GAMBIAE MOSQUITOS, Gastrointestinal Microbiome, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Biological Variation, Population, HABITATS, Larva, diversité génétique, Host-Pathogen Interactions, Metagenome, GENETIC DIVERSITY, IMMUNE-SYSTEM, Metagenomics, COMMUNITIES, AEDES-AEGYPTI, Research Article, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract: The adult phenotype of mosquitoes depends on the types of bacteria encountered environmentally during development., Conditions experienced during larval development of holometabolous insects can affect adult traits, but whether differences in the bacterial communities of larval development sites contribute to variation in the ability of insect vectors to transmit human pathogens is unknown. We addressed this question in the mosquito Aedes aegypti, a major arbovirus vector breeding in both sylvatic and domestic habitats in Sub-Saharan Africa. Targeted metagenomics revealed differing bacterial communities in the water of natural breeding sites in Gabon. Experimental exposure to different native bacterial isolates during larval development resulted in significant differences in pupation rate and adult body size but not life span. Larval exposure to an Enterobacteriaceae isolate resulted in decreased antibacterial activity in adult hemolymph and reduced dengue virus dissemination titer. Together, these data provide the proof of concept that larval exposure to different bacteria can drive variation in adult traits underlying vectorial capacity. Our study establishes a functional link between larval ecology, environmental microbes, and adult phenotypic variation in a holometabolous insect vector.
Published: 2017
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7. The predatory behavior of the Neotropical social wasp Polybia rejecta

Author: Héctor Rodríguez-Pérez, Frédéric Azémar, Bruno Corbara, James M. Carpenter, Alain Dejean, Laboratoire Ecologie Fonctionnelle et Environnement (LEFE), Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Observatoire Midi-Pyrénées (OMP), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Ecologie des forêts de Guyane (UMR ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Division of Invertebrate Zoology [New York], American Museum of Natural History (AMNH), Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Financial support for this study was provided by an 'Investissement d’Avenir' grants managed by the Agence Nationale de la Recherche (CEBA, ref. ANR-10-LABX-25-01)., ANR-10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), Laboratoire Ecologie Fonctionnelle et Environnement (ECOLAB), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Université des Antilles (UA)-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Microorganismes : Génome et Environnement - Clermont Auvergne (LMGE), Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), ANR-10-LABX-25-01/10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia(2010), Laboratoire Ecologie Fonctionnelle et Environnement - ECOLAB ( ECOLAB ), Institut National Polytechnique [Toulouse] ( INP ) -Université Paul Sabatier - Toulouse 3 ( UPS ) -Observatoire Midi-Pyrénées ( OMP ) -Centre National de la Recherche Scientifique ( CNRS ), Ecologie des forêts de Guyane ( ECOFOG ), Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ) -Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech-Université de Guyane ( UG ) -Centre National de la Recherche Scientifique ( CNRS ) -Université des Antilles ( UA ), Ecosystèmes, biodiversité, évolution [Rennes] ( ECOBIO ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -INEE-Observatoire des Sciences de l'Univers de Rennes ( OSUR ) -Centre National de la Recherche Scientifique ( CNRS ), American Museum of Natural History ( AMNH ), Laboratoire Microorganismes : Génome et Environnement - Clermont Auvergne ( LMGE ), Université Clermont Auvergne ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), and ANR-10-LABX-25-01/10-LABX-0025,CEBA,CEnter of the study of Biodiversity in Amazonia ( 2010 )
Subjects: 0106 biological sciences, Male, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Prey detection, ved/biology.organism_classification_rank.species, Wasps, Predation, Biology, 010603 evolutionary biology, 01 natural sciences, Nesting Behavior, Epiponini, Behavioral Neuroscience, Social Facilitation, Predatory behavior, Nest, Polistinae, [ SDV.EE.IEO ] Life Sciences [q-bio]/Ecology, environment/Symbiosis, Animals, Epiponine wasps, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, Vespidae, prey selection, Ecology, ved/biology, Polybia rejecta, General Medicine, Feeding Behavior, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 010602 entomology, Predatory Behavior, Visual Perception, Animal Science and Zoology, Female, Recruitment, Cues, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract: International audience; We experimentally studied the predatory behavior of Polybia rejecta (Vespidae, Polistinae, Epiponini) towards 2-88 mm-long insects attracted to a UV light trap. Foragers, which began to hunt at 6:30, selected 4-14 mm-long prey insects. Prey detection by sight by hovering wasps was confirmed using decoys. After the wasps landed and walked along a sinuous path, prey were detected by contact or from a distance (1-3cm). This was followed by seizure, stinging (contrarily to most other known cases), prey manipulation and retrieval. Prey that flew off might be caught in flight. The prey load, representing 30.7% of a forager's weight, was optimized by capturing up to six small prey or two medium-sized prey successively (both of which might be consumed in situ). The foragers cut off the wings of larger prey or cut them into two pieces and returned to gather the second piece. The handling time increased exponentially with the weight of the prey. Partial loading (i.e., retrieving a load much inferior to the maximum possible) was likely related to social facilitation, a form of nest-based recruitment that was demonstrated through the experimental elimination of local enhancement by removing foragers (both mechanisms favor the exploitation of favorable patches).
Published: 2017
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8. Cancer brings forward oviposition in the fly Drosophila melanogaster

Author: Lucas Léger, François Renaud, Jacques Montagne, Andreu Casali, Camille Jacqueline, Frédéric Thomas, Celine Moreno, Frederic Mery, Aurélie Tasiemski, Céline Boidin-Wichlacz, Dominique Faugere, Beata Ujvari, Audrey Arnal, Dorothée Missé, Benjamin Roche, Centre de Recherches Ecologiques et Evolutives sur le Cancer (MIVEGEC-CREEC), Processus Écologiques et Évolutifs au sein des Communautés (PEEC), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Deakin University, Burwood, Australia, Deakin University [Burwood], Laboratoire Evolution, Génomes et Spéciation [Gif-sur-Yvette] (LEGS), Institut de recherche pour le développement [IRD] : UR072-Centre National de la Recherche Scientifique (CNRS), Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 (Evo-Eco-Paléo (EEP)), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Croissance et métabolisme chez la Drosophile (METABO), Département Biologie Cellulaire (BioCell), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institute for Research in Biomedicine (IRB Barcelona), Unité de modélisation mathématique et informatique des systèmes complexes [Bondy] (UMMISCO), Institut de Recherche pour le Développement (IRD)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université de Yaoundé I-Institut de la francophonie pour l'informatique-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Université Gaston Bergé (Saint-Louis, Sénégal)-Université Cadi Ayyad [Marrakech] (UCA), Centre de Recherches Ecologiques et Evolutives sur le Cancer ( CREEC ), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle ( MIVEGEC ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ) -Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ), Laboratoire Evolution, Génomes et Spéciation [Gif-sur-Yvette] ( LEGS ), Institut de recherche pour le développement [IRD] : UR072-Centre National de la Recherche Scientifique ( CNRS ), Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 ( Evo-Eco-Paléo ), Université de Lille-Centre National de la Recherche Scientifique ( CNRS ), Croissance et métabolisme chez la Drosophile ( METABO ), Département Biologie Cellulaire ( BioCell ), Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Institut de Biologie Intégrative de la Cellule ( I2BC ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ) -Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ), Université Paris-Sud - Paris 11 ( UP11 ) -Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay-Centre National de la Recherche Scientifique ( CNRS ), Unité de modélisation mathématique et informatique des systèmes complexes [Bondy] ( UMMISCO ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut de la francophonie pour l'informatique-Université Cheikh Anta Diop ( UCAD ) -Université Gaston Bergé (Saint-Louis, Sénégal)-Universtié Yaoundé 1 (Cameroun)-University Cadi Ayyad ( UCA ), Évolution, Écologie et Paléontologie (Evo-Eco-Paleo) - UMR 8198 (Evo-Eco-Paléo), Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Université Pierre et Marie Curie - Paris 6 (UPMC)-Université de Yaoundé I-Institut de la francophonie pour l'informatique-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Université Gaston Bergé (Saint-Louis, Sénégal)-Université Cadi Ayyad [Marrakech] (UCA), and Université Cadi Ayyad [Marrakech] (UCA)-Université de Yaoundé I-Université Gaston Bergé (Saint-Louis, Sénégal)-Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD)-Institut de la francophonie pour l'informatique-Université Pierre et Marie Curie - Paris 6 (UPMC)
Subjects: 0106 biological sciences, 0301 basic medicine, [ SDV.BDLR.RS ] Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, media_common.quotation_subject, Period (gene), fecundity, Zoology, life-history strategy, 010603 evolutionary biology, 01 natural sciences, Life history theory, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, reproduction, 03 medical and health sciences, medicine, cancer, Allele, Ecology, Evolution, Behavior and Systematics, Nature and Landscape Conservation, media_common, Original Research, Genetics, Ecology, biology, Cancer, biology.organism_classification, medicine.disease, Fecundity, 3. Good health, Genetically modified organism, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 030104 developmental biology, Reproduction, Drosophila melanogaster, life‐history strategy
Abstract: International audience; Hosts often accelerate their reproductive effort in response to a parasitic infection, especially when their chances of future reproduction decrease with time from the onset of the infection. Because malignancies usually reduce survival, and hence potentially the fitness, it is expected that hosts with early cancer could have evolved to adjust their life-history traits to maximize their immediate reproductive effort. Despite the potential importance of these plastic responses, little attention has been devoted to explore how cancers influence animal reproduction. Here, we use an experimental setup, a colony of genetically modified flies Drosophila melanogaster which develop colorectal cancer in the anterior gut, to show the role of cancer in altering life-history traits. Specifically, we tested whether females adapt their reproductive strategy in response to harboring cancer. We found that flies with cancer reached the peak period of oviposition significantly earlier (i.e., 2 days) than healthy ones, while no difference in the length and extent of the fecundity peak was observed between the two groups of flies. Such compensatory responses to overcome the fitness-limiting effect of cancer could explain the persistence of inherited cancer-causing mutant alleles in the wild. K E Y W O R D S cancer, fecundity, life-history strategy, reproduction
Published: 2017
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9. Identification of sympatric cryptic species of Aedes albopictus subgroup in Vietnam: new perspectives in phylosymbiosis of insect vector

Author: Guillaume, Minard, Van, Tran Van, Florence Hélène, Tran, Christian, Melaun, Sven, Klimpel, Lisa Katharina, Koch, Khanh, Ly Huynh Kim, Trang, Huynh Thi Thuy, Huu, Tran Ngoc, Patrick, Potier, Patrick, Mavingui, Claire, Valiente Moro, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon, Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Senckenberg Biodiversity & Climate Research Centre, Institute of Ecology, Evolution and Diversity, Goethe-Universität Frankfurt am Main, Institut Pasteur d'Ho Chi Minh Ville, Réseau International des Instituts Pasteur (RIIP), Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Université Claude Bernard Lyon 1 ( UCBL ), Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Recherche Agronomique ( INRA ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Goethe University, Processus Infectieux en Milieu Insulaire Tropical ( PIMIT ), Université de la Réunion ( UR ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IRD-Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Senckenberg Biodiversity and Climate Research Centre (SBiK-F), Goethe-Universität Frankfurt am Main-Senckenberg – Leibniz Institution for Biodiversity and Earth System Research - Senckenberg Gesellschaft für Naturforschung, Leibniz Association-Leibniz Association, Centre National de la Recherche Scientifique (CNRS)-IRD-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), EC2CO CNRS, CMIRA Region Rhone-Alpes, ERA-NET BiodivERsA ANR-13-EBID-0007-01 FWF I-1437, DFG KL 2087/6-1, FR41 Bio-Environment and Health (University Lyon 1), Academy of Finland 273098 265641, Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Biosciences, Centre of Excellence in Metapopulation Research, and Life-history Evolution Research Group
Subjects: asian tiger mosquito, sibling species, wolbachia, microbiota, dysgonomonas, moustique, MITOCHONDRIAL-DNA, TRANSMISSION, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, viruses, DIVERSITY, Microbiota, Wolbachia, Sibling species, Dysgonomonas, Asian tiger mosquito, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, BACTERIAL MICROBIOTA, lcsh:Infectious and parasitic diseases, DIPTERA-CULICIDAE, ddc:590, microbiote, Aedes, RNA, Ribosomal, 16S, [ SDV.EE.IEO ] Life Sciences [q-bio]/Ecology, environment/Symbiosis, Animals, lcsh:RC109-216, Symbiosis, ENDOSYMBIONT, WOLBACHIA-PIPIENTIS, 1183 Plant biology, microbiology, virology, SEQUENCE ALIGNMENT, Bacteria, Research, Microbiology and Parasitology, fungi, virus diseases, Biodiversity, vietnam, Microbiologie et Parasitologie, Insect Vectors, Mitochondria, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Culicidae, 1181 Ecology, evolutionary biology, POPULATIONS, [ SDV.BID.SPT ] Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, CULEX-PIPIENS MOSQUITO, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract: Background The Aedes (Stegomyia) albopictus subgroup includes 11 cryptic species of which Ae. albopictus is the most widely distributed. Its global expansion associated with a documented vector competence for several emerging arboviruses raise obvious concerns in the recently colonized regions. While several studies have provided important insights regarding medical importance of Ae. albopicus, the investigations of the other sibling species are scarce. In Asia, indigenous populations within the Ae. albopictus subgroup can be found in sympatry. In the present study, we aimed to describe and compare molecular, morphological and bacterial symbionts composition among sympatric individuals from the Ae. albopictus subgroup inhabiting a Vietnamese protected area. Results Based on morphological structure of the cibarial armarture, we identified a cryptic species in the forest park at Bù Gia Mập in the south-eastern region of Vietnam. Analysis of nuclear (ITS1-5.8S-ITS2) and mitochondrial (cox1, nad5) markers confirmed the divergence between the cryptic species and Ae. albopictus. Analysis of midgut bacterial microbiota revealed a strong similarity among the two species with a notable difference; contrary to Ae. albopictus, the cryptic species did not harbour any Wolbachia infection. Conclusions These results could reflect either a recent invasion of Wolbachia in Ae. albopictus or alternatively a loss of this symbiont in the cryptic species. We argue that neglected species of the Ae. albopictus subgroup are of main importance in order to estimate variation of host-symbionts interactions across evolution. Electronic supplementary material The online version of this article (doi:10.1186/s13071-017-2202-9) contains supplementary material, which is available to authorized users.
Published: 2017
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10. Deep conservation of bivalve nacre proteins highlighted by shell matrix proteomics of the Unionoida Elliptio complanata and Villosa lienosa

Author: Jaison Arivalagan, Benjamin Marie, Arul Marie, Lucrèce Mathéron, Sophie Berland, Frédéric Marin, Gérard Bolbach, Molécules de Communication et Adaptation des Micro-Organismes ( MCAM ), Muséum National d'Histoire Naturelle ( MNHN ) -Centre National de la Recherche Scientifique ( CNRS ), Analyse, Interactions Moléculaires et Cellulaires ( LBM-E2 ), Laboratoire des biomolécules ( LBM UMR 7203 ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Département de Chimie - ENS Paris, École normale supérieure - Paris ( ENS Paris ) -École normale supérieure - Paris ( ENS Paris ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Département de Chimie - ENS Paris, École normale supérieure - Paris ( ENS Paris ) -École normale supérieure - Paris ( ENS Paris ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Biologie des Organismes et Ecosystèmes Aquatiques ( BOREA ), Université des Antilles ( UA ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut de Recherche pour le Développement ( IRD ) -Muséum National d'Histoire Naturelle ( MNHN ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), Biogéosciences [Dijon] ( BGS ), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Funding from the Marie Curie European Union Seventh Framework Programme under grant agreement no. 605051., Molécules de Communication et Adaptation des Micro-Organismes (MCAM), Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Analyse, Interactions Moléculaires et Cellulaires (LBM-E2), Laboratoire des biomolécules (LBM UMR 7203), Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Biogéosciences [UMR 6282] (BGS), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC), Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Biogéosciences [UMR 6282] [Dijon] (BGS), and Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)
Subjects: Unionidae, 0301 basic medicine, Unionoida, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Villosa lienosa, Biomedical Engineering, Biophysics, Life Sciences–Earth Science interface, Bioengineering, Biology, Proteomics, Biochemistry, bivalve, Evolution, Molecular, Biomaterials, 03 medical and health sciences, Paleontology, Calcification, Physiologic, proteomics, Animal Shells, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], shell nacre, Shell matrix, Animals, calcium carbonate, 14. Life underwater, Nacre, Mantle (mollusc), chemistry.chemical_classification, Extracellular Matrix Proteins, Elliptio, biology.organism_classification, biomineralization, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 030104 developmental biology, chemistry, [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], organic matrix proteins, Glycoprotein, Biotechnology, Biomineralization
Abstract: The formation of the molluscan shell nacre is regulated to a large extent by a matrix of extracellular macromolecules that are secreted by the shell-forming tissue, the mantle. This so-called ‘calcifying matrix’ is a complex mixture of proteins, glycoproteins and polysaccharides that is assembled and occluded within the mineral phase during the calcification process. Better molecular-level characterization of the substances that regulate nacre formation is still required. Notable advances in expressed tag sequencing of freshwater mussels, such as Elliptio complanata and Villosa lienosa , provide a pre-requisite to further characterize bivalve nacre proteins by a proteomic approach. In this study, we have identified a total of 48 different proteins from the insoluble matrices of the nacre, 31 of which are common to both E. complanata and V. lienosa . A few of these proteins, such as PIF, MSI60, CA, shematrin-like, Kunitz-like, LamG, chitin-binding-containing proteins, together with A-, D-, G-, M- and Q-rich proteins, appear to be analogues, if not true homologues, of proteins previously described from the pearl oyster or the edible mussel nacre matrices, thus forming a remarkable list of deeply conserved nacre proteins. This work constitutes a comprehensive nacre proteomic study of non-pteriomorphid bivalves that has enabled us to describe the molecular basis of a deeply conserved biomineralization toolkit among nacreous shell-bearing bivalves, with regard to proteins associated with other shell microstructures, with those of other mollusc classes (gastropods, cephalopods) and, finally, with other lophotrochozoans (brachiopods).
Published: 2017
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11. Bacterial diversity of cosmopolitan Culex pipiens and invasive Aedes japonicus from Germany

Author: Manuela Spaeth, Chinhda Xoumpholphakdy, Antje Steinbrink, Patrick Mavingui, Sina Zotzmann, Kathrin Schleich, Claire Valiente Moro, Sven Klimpel, Felix Frantzmann, Institute of Ecology, Evolution and Diversity, Goethe-Universität Frankfurt am Main, Senckenberg Biodiversity and Climate Research Centre (SBiK-F), Goethe-Universität Frankfurt am Main-Senckenberg – Leibniz Institution for Biodiversity and Earth System Research - Senckenberg Gesellschaft für Naturforschung, Leibniz Association-Leibniz Association, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Univ, Réunion, Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Centre National de la Recherche Scientifique (CNRS)-IRD-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), Senckenberg Biodiversity & Climate Research Centre, Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Goethe University, Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Processus Infectieux en Milieu Insulaire Tropical ( PIMIT ), and Université de la Réunion ( UR ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IRD-Centre National de la Recherche Scientifique ( CNRS )
Subjects: 0301 basic medicine, Culex, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 030106 microbiology, 030231 tropical medicine, Zoology, Biology, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, DNA, Ribosomal, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Aedes, Aedes japonicus, Germany, Culex pipiens, Botany, [ SDV.EE.IEO ] Life Sciences [q-bio]/Ecology, environment/Symbiosis, Animals, Polymerase chain reaction, General Veterinary, Bacteria, Phylum, Microbiota, fungi, General Medicine, [SDV.EE.IEO] Life Sciences [q-bio]/Ecology, environment/Symbiosis, biology.organism_classification, 16S ribosomal RNA, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 3. Good health, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Infectious Diseases, Bacterial diversity, Insect Science, [SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Parasitology, Female, Proteobacteria, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Symbiotic bacteria, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract: International audience; Symbiotic bacteria have gained significant attention in recent years. For example, microbiota of some mosquito species seems to influence the development and transmission of pathogens. Furthermore, several attempts using bacteria as a paratransgenetic tool have been made in order to assist the control of mosquito-borne diseases. In this study, we examined the bacterial diversity of wild-caught adult Culex (Cx.) pipiens and laboratory-reared adult Aedes japonicus (Ae. japonicus) in Germany using a culture-independent method. Genomic DNA was extracted from each specimen and submitted to PCR amplification of eubacterial 16S rDNA. After the cloning reaction , 28 bacterial transformants per sample containing the 16S rDNA inserts were selected per each sample for se-quencing. The analysed specimens of Cx. pipiens as well as of Ae. japonicus showed a diverse bacterial community including some common bacterial genera. Blast analysis allowed to identify 21 bacterial genera belonging to 2 phyla among the 23 specimens of Cx. pipiens. The 14 analysed Ae. japonicus revealed 11 bacterial genera belonging to 3 phyla. In both mosquito species, identified isolates were mainly Proteobacteria. Only 4 of the bacterial genera were found in both mosquito species, with the most prevalent genera Sphingomonas and Rahnella in Cx. pipiens and in Ae. japonicus respectively. Most of the bacterial genera found in our study have been identified in other mosquito species before. Due to the currently scarce data situation, ongoing examinations on the very abundant bacterial genera or species are strongly required to determine their relevance for the biology and adaptiveness of mosquitoes including pathogen-host relationship.
Published: 2017
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12. Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon

Author: Saucereau, Yoann, Valiente Moro, Claire, Dieryckx, Cindy, Dupuy, Jean-William, Tran, Florence-Hélène, Girard, Vincent, Potier, Patrick, Mavingui, Patrick, Institut National de la Recherche Agronomique (INRA), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Université de Lyon, Université Claude Bernard Lyon 1 (UCBL), Centre National de la Recherche Scientifique (CNRS), Génomique fonctionnelle des champignons pathogènes des plantes (FungiPath), Microbiologie, adaptation et pathogénie (MAP), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Centre Génomique Fonctionnelle Bordeaux [Bordeaux] (CGFB), Institut Polytechnique de Bordeaux-Université de Bordeaux Ségalen [Bordeaux 2], Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Centre National de la Recherche Scientifique (CNRS)-IRD-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Region Rhone-Alpes, ERA-NET BiodivERsA [ANR-13-EBID-0007-01], [FWF I-1437 ], DFG part of BiodivERsA [KL 2087/6-1], Institut National de la Recherche Agronomique ( INRA ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Université Claude Bernard Lyon 1 ( UCBL ), Centre National de la Recherche Scientifique ( CNRS ), Microbiologie, adaptation et pathogénie ( MAP ), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon ( INSA Lyon ), Université de Lyon-Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS ), Centre Génomique Fonctionnelle Bordeaux [Bordeaux] ( CGFB ), Processus Infectieux en Milieu Insulaire Tropical ( PIMIT ), Université de la Réunion ( UR ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IRD-Centre National de la Recherche Scientifique ( CNRS ), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL)
Subjects: Proteomics, chikungunya, lcsh:QH426-470, Proteome, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, lcsh:Biotechnology, viruses, Biotechnologies, Cell Line, chromatographie en phase liquide, [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology, Aedes, spectrométrie de masse, lcsh:TP248.13-248.65, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], parasitic diseases, [ SDV.EE.IEO ] Life Sciences [q-bio]/Ecology, environment/Symbiosis, Animals, protéome, glycolyse, électrophorèse, miRNA, virus diseases, Aedes albopictus, inhibition, Viral inhibition, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, lcsh:Genetics, Metabolism, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], virus dengue type 2, micro arn, Wolbachia, Glycolysis, Arboviruses, Research Article, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract: Background Aedes albopictus is a vector of arboviruses that cause severe diseases in humans such as Chikungunya, Dengue and Zika fevers. The vector competence of Ae. albopictus varies depending on the mosquito population involved and the virus transmitted. Wolbachia infection status in believed to be among key elements that determine viral transmission efficiency. Little is known about the cellular functions mobilized in Ae. albopictus during co-infection by Wolbachia and a given arbovirus. To decipher this tripartite interaction at the molecular level, we performed a proteome analysis in Ae. albopictus C6/36 cells mono-infected by Wolbachia wAlbB strain or Chikungunya virus (CHIKV), and bi-infected. Results We first confirmed significant inhibition of CHIKV by Wolbachia. Using two-dimensional gel electrophoresis followed by nano liquid chromatography coupled with tandem mass spectrometry, we identified 600 unique differentially expressed proteins mostly related to glycolysis, translation and protein metabolism. Wolbachia infection had greater impact on cellular functions than CHIKV infection, inducing either up or down-regulation of proteins associated with metabolic processes such as glycolysis and ATP metabolism, or structural glycoproteins and capsid proteins in the case of bi-infection with CHIKV. CHIKV infection inhibited expression of proteins linked with the processes of transcription, translation, lipid storage and miRNA pathways. Conclusions The results of our proteome profiling have provided new insights into the molecular pathways involved in tripartite Ae. albopictus-Wolbachia-CHIKV interaction and may help defining targets for the better implementation of Wolbachia-based strategies for disease transmission control. Electronic supplementary material The online version of this article (doi:10.1186/s12864-017-3985-y) contains supplementary material, which is available to authorized users.
Published: 2017
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13. Free flight odor tracking in Drosophila: Effect of wing chemosensors, sex and pheromonal gene regulation

Author: Benjamin Houot, Vincent Gigot, Alain Robichon, Jean-François Ferveur, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (INSB) Burgundy Regional Council (PARI) Universite de Bourgogne CEFIPRA 5103-A CONICYT MEC 80140013, ANR-11-BSV7-0017,GUSTAILE2011,Rôle des chimiosenseurs de l'aile sur le comportement d'orientation de la mouche(2011), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Institut Sophia Agrobiotech [Sophia Antipolis] ( ISA ), Centre National de la Recherche Scientifique ( CNRS ) -Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut National de la Recherche Agronomique ( INRA ), ANR-11-BSV7-0017,GUSTAILE2011,Rôle des chimiosenseurs de l'aile sur le comportement d'orientation de la mouche ( 2011 ), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA)
Subjects: Fatty Acid Desaturases, Male, exploratory-behavior, melanogaster, animal structures, coordination, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, neurons, system, Pheromones, Article, orientation, Animals, Genetically Modified, expression, Animals, Drosophila Proteins, Wings, Animal, Sex Attractants, Sex Characteristics, communication, fungi, Sense Organs, Smell, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, modulation, Drosophila melanogaster, Gene Expression Regulation, Flight, Animal, Odorants, fruit-flies, Female
Abstract: indexation en coursSupplementary information accompanies this paper at http://www.nature.com/srep; International audience; The evolution of powered flight in insects had major consequences for global biodiversity and involved the acquisition of adaptive processes allowing individuals to disperse to new ecological niches. Flies use both vision and olfactory input from their antennae to guide their flight; chemosensors on fly wings have been described, but their function remains mysterious. We studied Drosophila flight in a wind tunnel. By genetically manipulating wing chemosensors, we show that these structures play an essential role in flight performance with a sex-specific effect. Pheromonal systems are also involved in Drosophila flight guidance: transgenic expression of the pheromone production and detection gene, desat1, produced low, rapid flight that was absent in control flies. Our study suggests that the sex-specific modulation of free-flight odor tracking depends on gene expression in various fly tissues including wings and pheromonal-related tissues.
Published: 2017
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14. L’attraction exercée par la flore microbienne de la peau sur le moustique tigre : La microflore cutanée comme source de molécules attractantes pour le moustique tigre Aedes albopictus : application au développement de stratégies innovantes en lutte anti-vectorielle

Author: VALIENTE-MORO, Claire, Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), PNR EST, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), and Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)
Subjects: [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, bactérie, moustique, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, kairomone, insecticide, Aedes albopictus, insecte, [ SDV.EE.SANT ] Life Sciences [q-bio]/Ecology, environment/Health, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, peau, méthode alternative, résistance traitement, pesticide
Abstract: L’évolution des populations de moustiques dépend de différents facteurs (alimentaire, non alimentaire, reproductif) qui vont conditionner à la fois leur comportement et leur écologie. Parmi eux, les odeurs, l’olfaction étant le principal sens par lequel les moustiques localisent leurs hôtes cibles. Récemment, le développement de l’écologie chimique a permis de formuler de nouvelles hypothèses pour expliquer le tropisme des moustiques vis-à-vis de l’homme. Il semblerait que les sensilles chémoréceptrices (organes sensoriels) au niveau des antennes répondent, chez certains insectes hématophages (y compris les moustiques), à divers composés organiques volatils présents sur la peau de l’homme et dans sa sueur (ex. ammonium, acide lactique…) qui agiraient comme des stimuli.
Published: 2017

15. Lateralization of Eye Use in Cuttlefish: Opposite Direction for Anti-Predatory and Predatory Behaviors

Author: Roger T. Hanlon, Alexandra K. Schnell, Aïcha Benkada, Christelle Jozet-Alves, Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Marine Biological Laboratory (MBL), University of Chicago, Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), and Marine Biological Laboratory ( MBL )
Subjects: 0106 biological sciences, Cuttlefish, genetic structures, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Physiology, [SDV]Life Sciences [q-bio], 01 natural sciences, lcsh:Physiology, Visual processing, 0302 clinical medicine, wild, Sepia, cephalopod, Original Research, lcsh:QP1-981, hemispheric specialization, Functional specialization, octopus-vulgaris, advantages, Anatomy, Visual field, medicine.anatomical_structure, [ SCCO.NEUR ] Cognitive science/Neuroscience, brain lateralization, asymmetry, vision, invertebrate, visual lateralization, Biology, 010603 evolutionary biology, Lateralization of brain function, 03 medical and health sciences, brain specialization, lizard podarcis-muralis, Physiology (medical), evolution, medicine, 14. Life underwater, [ SDV ] Life Sciences [q-bio], [SCCO.NEUR]Cognitive science/Neuroscience, biology.organism_classification, functional lateralization, disadvantages, Lobe, eye use, Cephalopod, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Neuroscience, 030217 neurology & neurosurgery
Abstract: WOS:000389670100001; International audience; Vertebrates with laterally placed eyes typically exhibit preferential eye use for ecological activities such as scanning for predators or prey. Processing visual information predominately through the left or right visual field has been associated with specialized function of the left and right brain. Lateralized vertebrates often share a general pattern of lateralized brain function at the population level, whereby the left hemisphere controls routine behaviors and the right hemisphere controls emergency responses. Recent studies have shown evidence of preferential eye use in some invertebrates, but whether the visual fields are predominately associated with specific ecological activities remains untested. We used the European common cuttlefish. Sepia officinalis, to investigate whether the visual field they use is the same, or different, during anti-predatory, and predatory behavior. To test for lateralization of anti-predatory behavior, individual cuttlefish were placed in a new environment with opaque walls, thereby obliging them to choose which eye to orient away from the opaque wall to scan for potential predators (i.e., vigilant scanning). To test for lateralization of predatory behavior, individual cuttlefish were placed in the apex of an isosceles triangular arena and presented with two shrimp in opposite vertexes, thus requiring the cuttlefish to choose between attacking a prey item to the left or to the right of them. Cuttlefish were significantly more likely to favor the left visual field to scan for potential predators and the right visual field for prey attack. Moreover, individual cuttlefish that were leftward directed for vigilant scanning were predominately rightward directed for prey attack. Lateralized individuals also showed faster decision-making when presented with prey simultaneously. Cuttlefish appear to have opposite directions of lateralization for anti-predatory and predatory behavior, suggesting that there is functional specialization of each optic lobe (i.e., brain structures implicated in visual processing). These results are discussed in relation to the role of lateralized brain function and the evolution of population level lateralization.
Published: 2016
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16. Feeding choice and predation pressure of two invasive gammarids, Gammarus tigrinus and Dikerogammarus villosus, under increasing temperature

Author: Vincent Médoc, Laura Pellan, Thierry Spataro, David Renault, Christophe Piscart, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Institut d'écologie et des sciences de l'environnement de Paris (IEES (UMR_7618 / UMR_D_242 / UMR_A_1392 / UM_113) ), Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), AgroParisTech, Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Institut d'écologie et des sciences de l'environnement de Paris (iEES Paris ), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecosystèmes, biodiversité, évolution [Rennes] ( ECOBIO ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -INEE-Observatoire des Sciences de l'Univers de Rennes ( OSUR ) -Centre National de la Recherche Scientifique ( CNRS ), Biogéosciences [Dijon] ( BGS ), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Institut d'écologie et des sciences de l'environnement de Paris ( IEES ), Institut National de la Recherche Agronomique ( INRA ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 ) -Centre National de la Recherche Scientifique ( CNRS ), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2), and Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Predatory impact, Foraging, Functional response, Aquatic Science, 010603 evolutionary biology, 01 natural sciences, Predation, Trophic ecology, Gammarus, [ SDV.EE.BIO ] Life Sciences [q-bio]/Ecology, environment/Bioclimatology, Climate change, 14. Life underwater, Biological invasions, ComputingMilieux_MISCELLANEOUS, Herbivore, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, biology, Ecology, 010604 marine biology & hydrobiology, Detritivore, Dikerogammarus villosus, Amphipods, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Ectotherm, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, [SDV.EE.BIO]Life Sciences [q-bio]/Ecology, environment/Bioclimatology
Abstract: International audience; In most European freshwater ecosystems, the invasive gammarids Gammarus tigrinus and Dikerogammarus villosus strongly impair recipient communities through predation of a wide range of native invertebrates. Due to the effects of temperature on both the physiology and the behaviour of such ectotherms, understanding how global warming may influence their ecological impact is a research priority. These species were therefore exposed to three different food types to determine their detritivorous, herbivorous and carnivorous characters, and predation was measured characterizing the Holling’s functional response. The effect of increasing water temperatures (15, 20, 25°C) on both the food choice and predatory activities was investigated. Both species showed a significant preference for animal tissues at all temperatures. The total food intake increased with temperature for G. tigrinus but did not change for D. villosus, which may result from specific species differences in metabolic requirements. The consumption of live prey strongly increased with temperature. The main differences were an increased searching efficiency in G. tigrinus and a decreased handling time in D. villosus as temperature increased, which may result from differences in foraging strategies. These results suggest that climate change is likely to increase the predation pressure of both invasive gammarids on prey species.
Published: 2016
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17. Functional Gustatory Role of Chemoreceptors in Drosophila Wings

Author: Maria Capovilla, Alain Robichon, Hussein Raad, Neil Ledger, Jean-François Ferveur, Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), the ANR ‘‘blanc’’, acronym: ‘‘ Gustaile’’, and by the French Government (National Research Agency, ANR) through the LABEX SIGNALIFE program (reference # ANR-11-LABX-0028-01)., Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Robichon, Alain, Institut Sophia Agrobiotech [Sophia Antipolis] ( ISA ), Centre National de la Recherche Scientifique ( CNRS ) -Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut National de la Recherche Agronomique ( INRA ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), and Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS )
Subjects: 0301 basic medicine, melanogaster, Taste, Chemoreceptor, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, neurons, Insect, male courtship behavior, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Animals, Genetically Modified, 0302 clinical medicine, Cytosol, Conditioning, Psychological, Drosophila Proteins, Wings, Animal, Sensilla, lcsh:QH301-705.5, media_common, Animal biology, biology, Behavior, Animal, Anatomy, transduction, bitter, Chemoreceptor Cells, Drosophila melanogaster, Alimentation et Nutrition, candidate taste receptors, apis-mellifera, insect flight, gene, trasnsduction, odorant, animal structures, media_common.quotation_subject, Carbohydrates, Time-Lapse Imaging, General Biochemistry, Genetics and Molecular Biology, Fluorescence, 03 medical and health sciences, Biologie animale, Animals, Food and Nutrition, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Calcium Signaling, RNA, Messenger, Drosophila, Wing, fungi, Neurosciences, Water, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BDD.MOR]Life Sciences [q-bio]/Development Biology/Morphogenesis, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SDV.GEN.GA]Life Sciences [q-bio]/Genetics/Animal genetics, 030104 developmental biology, lcsh:Biology (General), Food, Neurons and Cognition, Calcium, Neuroscience, 030217 neurology & neurosurgery
Abstract: Summary: Neuroanatomical evidence argues for the presence of taste sensilla in Drosophila wings; however, the taste physiology of insect wings remains hypothetical, and a comprehensive link to mechanical functions, such as flight, wing flapping, and grooming, is lacking. Our data show that the sensilla of the Drosophila anterior wing margin respond to both sweet and bitter molecules through an increase in cytosolic Ca2+ levels. Conversely, genetically modified flies presenting a wing-specific reduction in chemosensory cells show severe defects in both wing taste signaling and the exploratory guidance associated with chemodetection. In Drosophila, the chemodetection machinery includes mechanical grooming, which facilitates the contact between tastants and wing chemoreceptors, and the vibrations of flapping wings that nebulize volatile molecules as carboxylic acids. Together, these data demonstrate that the Drosophila wing chemosensory sensilla are a functional taste organ and that they may have a role in the exploration of ecological niches. : The function of Drosophila wing chemosensilla is poorly understood. GFP gene reporter assays and electrophysiology are hampered by the nano-architecture of bristles and dense chitin. Raad et al. report that the wing taste organ responds to bitter and sugar stimuli and is critical for exploration of ecological niches.
Published: 2016
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18. The Effect of an Artificial Incubation Environment on Hatchling Size and Behavior in the Cuttlefish, Sepia Officinalis

Author: O Brien, C. E., Bowie, M., Billard, P., Darmaillacq, A. S., christelle jozet-alves, Benhaim, D., Basuyaux, O., Dickel, L., Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Normandie Université ( NU ), Hólar University College, ETHOS, UMR6552, Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), and Normandie Université (NU)
Subjects: prey-capture, [ SDV ] Life Sciences [q-bio], [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SCCO.NEUR]Cognitive science/Neuroscience, [SDV]Life Sciences [q-bio], growth, [SCCO.NEUR] Cognitive science/Neuroscience, consecutive generations, perception, postembryonic maturation, laboratory environment, behavioral plasticity, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SDV] Life Sciences [q-bio], memory, body patterning, body patterns, early experience, embryonic experience, [ SCCO.NEUR ] Cognitive science/Neuroscience, [SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, cuttlefish culture, cephalopoda, predation
Abstract: WOS:000379002800010; International audience; A great deal is known about development in Sepia officinalis; however, much of this knowledge comes from animals incubated in laboratory conditions. Since cuttlefish are behaviorally plastic and known as embryos to perceive environmental stimuli from within the egg, we wondered if they are affected by incubation environment and thus whether laboratory-incubated cuttlefish exhibit natural behavior. We investigated the effects of incubation environment on hatchling size, defense and predation behavior in Sepia officinalis by comparing artificially-incubated hatchlings to naturally-incubated ones. Contrary to our expectations, no significant differences were apparent in hatchling size, disruptive body patterning and predation. A significant difference did exist between groups in one type of body patterning: Artificially-incubated hatchlings appeared to be better at producing a uniform body pattern than naturally-incubated individuals, possibly due to their prenatal experience with a homogeneous artificial substrate. This difference suggests some caution when interpreting experiments utilizing laboratory-incubated hatchlings, but overall, the limited effect of artificial incubation conditions demonstrated in this experiment bolsters confidence in previous behavioral results. These results are also promising for cuttlefish culture; eggs that would otherwise be lost as bycatch could instead be cultured artificially.
Published: 2016

19. Co-infection of Ticks: The Rule Rather Than the Exception

Author: Elise Vaumourin, Elodie Devillers, Gwenaël Vourc’h, Patrick Gasqui, Lorraine Michelet, Patrick Mavingui, Muriel Vayssier-Taussat, Florence Hélène Tran, Jean-François Cosson, Sara Moutailler, Claire Valiente Moro, Van Tran Van, Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche d'Épidémiologie Animale (UR EpiA), Institut National de la Recherche Agronomique (INRA), Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IRD-Centre National de la Recherche Scientifique (CNRS), Cyclotron Réunion Océan Indien (CYROI), Université de La Réunion (UR)-Centre Hospitalier Universitaire de La Réunion (CHU La Réunion), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Ecole Nationale Vétérinaire de Lyon (ENVL), Centre National de la Recherche Scientifique (CNRS)-IRD-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de La Réunion (UR), Biologie moléculaire et immunologie parasitaires et fongiques ( BIPAR ), Université Paris-Est Créteil Val-de-Marne - Paris 12 ( UPEC UP12 ) -AFSSA-Ecole Nationale Vétérinaire d'Alfort-Institut National de la Recherche Agronomique ( INRA ), Ecologie microbienne ( EM ), Centre National de la Recherche Scientifique ( CNRS ) -Ecole Nationale Vétérinaire de Lyon ( ENVL ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -VetAgro Sup ( VAS ), Épidémiologie des maladies animales et zoonotiques ( UMR EPIA ), Institut National de la Recherche Agronomique ( INRA ), Processus Infectieux en Milieu Insulaire Tropical ( PIMIT ), Université de la Réunion ( UR ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -IRD-Centre National de la Recherche Scientifique ( CNRS ), Cyclotron Réunion Océan Indien ( CYROI ), Université de la Réunion ( UR ) -Centre Hospitalier Universitaire de La Réunion ( CHU La Réunion ), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Vétérinaire de Lyon (ENVL)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Épidémiologie des Maladies Animales et Zoonotiques - UMR 346 (EPIA), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Univ, Réunion, and École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie
Subjects: 0301 basic medicine, Bacterial Diseases, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Epidemiology, Disease Vectors, Pathology and Laboratory Medicine, 0302 clinical medicine, Lyme disease, Ticks, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, RNA, Ribosomal, 16S, Theileria, [ SDV.EE.IEO ] Life Sciences [q-bio]/Ecology, environment/Symbiosis, Medicine and Health Sciences, Pathogen, Flowering Plants, Lyme Disease, biology, Transmission (medicine), Coinfection, lcsh:Public aspects of medicine, Plants, 3. Good health, Bacterial Pathogens, [ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, RNA, Bacterial, Infectious Diseases, Medical Microbiology, [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Female, France, Pathogens, RNA, Protozoan, Research Article, DNA, Bacterial, lcsh:Arctic medicine. Tropical medicine, Ixodes ricinus, Arthropoda, Borrelia Burgdorferi, lcsh:RC955-962, 030231 tropical medicine, Babesia, Microbiology, 03 medical and health sciences, Rheumatology, Virology, Arachnida, parasitic diseases, medicine, Animals, Microbial Pathogens, Ixodes, Bacteria, Ricinus, Borrelia, Public Health, Environmental and Occupational Health, Organisms, Biology and Life Sciences, lcsh:RA1-1270, [SDV.EE.IEO] Life Sciences [q-bio]/Ecology, environment/Symbiosis, DNA, Protozoan, biology.organism_classification, medicine.disease, bacterial infections and mycoses, Invertebrates, Borrelia Infection, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 030104 developmental biology, Co-Infections, [SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Animal Distribution, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract: Introduction Ticks are the most common arthropod vectors of both human and animal diseases in Europe, and the Ixodes ricinus tick species is able to transmit a large number of bacteria, viruses and parasites. Ticks may also be co-infected with several pathogens, with a subsequent high likelihood of co-transmission to humans or animals. However few data exist regarding co-infection prevalences, and these studies only focus on certain well-known pathogens. In addition to pathogens, ticks also carry symbionts that may play important roles in tick biology, and could interfere with pathogen maintenance and transmission. In this study we evaluated the prevalence of 38 pathogens and four symbionts and their co-infection levels as well as possible interactions between pathogens, or between pathogens and symbionts. Methodology/principal findings A total of 267 Ixodes ricinus female specimens were collected in the French Ardennes and analyzed by high-throughput real-time PCR for the presence of 37 pathogens (bacteria and parasites), by rRT-PCR to detect the presence of Tick-Borne encephalitis virus (TBEV) and by nested PCR to detect four symbionts. Possible multipartite interactions between pathogens, or between pathogens and symbionts were statistically evaluated. Among the infected ticks, 45% were co-infected, and carried up to five different pathogens. When adding symbiont prevalences, all ticks were infected by at least one microorganism, and up to eight microorganisms were identified in the same tick. When considering possible interactions between pathogens, the results suggested a strong association between Borrelia garinii and B. afzelii, whereas there were no significant interactions between symbionts and pathogens. Conclusion/significance Our study reveals high pathogen co-infection rates in ticks, raising questions about possible co-transmission of these agents to humans or animals, and their consequences to human and animal health. We also demonstrated high prevalence rates of symbionts co-existing with pathogens, opening new avenues of enquiry regarding their effects on pathogen transmission and vector competence., Author Summary Ticks transmit more pathogens than any other arthropod, and one single species can transmit a large variety of bacteria and parasites. Because co-infection might be much more common than previously thought, we evaluated the prevalence of 38 known or neglected tick-borne pathogens in Ixodes ricinus ticks. Our results demonstrated that co-infection occurred in almost half of the infected ticks, and that ticks could be infected with up to five pathogens. Moreover, as it is well established that symbionts can affect pathogen transmission in arthropods, we also evaluated the prevalence of four symbiont species and demonstrated that all ticks were infected by at least one microorganism. This work highlights the co-infection phenomenon in ticks, which may have important implications for human and animal health, emphasizing the need for new diagnostic tests better adapted to tick-borne diseases. Finally, the high co-occurrence of symbionts and pathogens in ticks, reveals the necessity to also account for these interactions in the development of new alternative strategies to control ticks and tick-borne disease.
Published: 2016
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20. How differential management strategies affect Ips typographus L. dispersal

Author: Bertheau Coralie, Krumböck Susanne, Moodley Yoshan, Montano Valeria, Okrouhlík Jan, Doležal Petr, Stauffer Christian, Department of Ecology and Evolution, University of Lausanne, Université de Lausanne (UNIL), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institute of Forest Ecology, Universität für Bodenkultur (BOKU), Biology Center, AS CR, Institute of Entomology, Université de Lausanne ( UNIL ), Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), and Institute of Forest Ecology, Universität für Bodenkultur ( BOKU )
Subjects: 0106 biological sciences, 0301 basic medicine, Ips typographus, Bark beetle, Ecology, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Population genetics, Forestry, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, 15. Life on land, Management, Monitoring, Policy and Law, Biology, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, Gene flow, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 03 medical and health sciences, 030104 developmental biology, [ SDV.BID.EVO ] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Biological dispersal, ComputingMilieux_MISCELLANEOUS, Nature and Landscape Conservation
Abstract: Bark beetle outbreaks have a devastating effect on economically important forests worldwide, thus requiring extensive application of management control strategies. The presence of unmanaged protected areas in close proximity to managed forests can instigate concerns that bark beetle infestations may spread from unmanaged into managed stands. We studied the impact of differential management of forest stands on the dispersal dynamics of the European spruce bark beetle, Ips typographus, making use of inferential population genetics on mitochondrial and nuclear genomes. Bayesian inferences of migration rates and a most parsimonious dispersal tree show that outgoing gene flow was consistently higher from managed to unmanaged areas. Reason for that is likely the thorough removal of potential breeding material in managed forests and thus the dispersal of the base stock beetles from these areas to unmanaged areas where breeding material is available. Our study suggests that the potential threat posed by unmanaged to managed forests in regard to I. typographus infestation needs to be carefully re-considered.
Published: 2016
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21. Chalky versus foliated: a discriminant immunogold labelling of shell microstructures in the edible oyster Crassostrea gigas

Author: Nathalie Guichard, Franck Lartaud, Vincent Mouchi, Marc de Rafélis, Frédéric Marin, Cédric Broussard, Françoise Immel, Quentin Crowley, School of Natural Sciences, Trinity College Dublin, Institut des Sciences de la Terre de Paris (iSTeP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecogéochimie des environnements benthiques (LECOB), Observatoire océanologique de Banyuls (OOB), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Biogéosciences [UMR 6282] [Dijon] (BGS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire SUBATECH Nantes (SUBATECH), Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Nantes (UN)-Mines Nantes (Mines Nantes), Laboratoire de biogenèse membranaire (LBM), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Géosciences Environnement Toulouse (GET), Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Biogéosciences [UMR 6282] (BGS), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Mines Nantes (Mines Nantes)-Université de Nantes (UN)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), HAL-UPMC, Gestionnaire, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Midi-Pyrénées (OMP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de la Terre de Paris ( iSTeP ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'Ecogéochimie des environnements benthiques ( LECOB ), Centre National de la Recherche Scientifique ( CNRS ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ), Biogéosciences [Dijon] ( BGS ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire SUBATECH Nantes ( SUBATECH ), Mines Nantes ( Mines Nantes ) -Université de Nantes ( UN ) -Institut National de Physique Nucléaire et de Physique des Particules du CNRS ( IN2P3 ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de biogenèse membranaire ( LBM ), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique ( CNRS ), Géosciences Environnement Toulouse ( GET ), Institut de Recherche pour le Développement ( IRD ) -Université Toulouse III - Paul Sabatier ( UPS ), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées ( OMP ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), and Institut de Recherche pour le Développement ( IRD ) -Université Paul Sabatier - Toulouse 3 ( UPS ) -Observatoire Midi-Pyrénées ( OMP ) -Centre National de la Recherche Scientifique ( CNRS )
Subjects: 0301 basic medicine, 010506 paleontology, Oyster, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Mineralogy, [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, Aquatic Science, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Labelling, biology.animal, Mantle (mollusc), Ecology, Evolution, Behavior and Systematics, 0105 earth and related environmental sciences, Calcite, Ecology, biology, Differential staining, [ SDU.STU ] Sciences of the Universe [physics]/Earth Sciences, Immunogold labelling, biology.organism_classification, Microstructure, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 030104 developmental biology, chemistry, Biophysics, [SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SDU.STU] Sciences of the Universe [physics]/Earth Sciences, Crassostrea
Abstract: International audience; Mollusc shells are organic–inorganic biocomposites, arranged in a limited number of superimposed calcified layers that generally exhibit very different organization of their crystallites. Because of their attractive mechanical and crystallographic properties, these shell layers have been the focus of several physical and biochemical characterizations. In particular, recent proteomic data obtained from individual layers suggest that their protein contents are different. However, the direct visual evidence that some macromolecular components are layer-specific is rather tenuous. This paper is based on a non-conventional immunogold labelling approach to localize proteins in the shell of the edible oyster Crassostrea gigas. The shell microstructure of this model organism is predominantly composed of foliated calcite, interspersed by discontinuous pockets of ‘chalky layers’, a porous microstructure typical of bivalves of the ostreid family. By developing a polyclonal antibody (in two rats) elicited against a proteinaceous shell fraction, we obtained differential staining of the two microstructures. We assert that our labelling is microstructure discriminant. The difference in labelling of the two shell microstructures suggests either that they are formed by a variation of the secretory repertoire of the shell-forming cells of the calcifying mantle epithelium or that the chalky layer may be formed via a completely different mechanism. Our results allow a first glimpse on the subtle regulatory mechanisms that drive the process of chalky and foliated layers deposition.
Published: 2016
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22. Guidelines for the Care and Welfare of Cephalopods in Research –A consensus based on an initiative by CephRes, FELASA and the Boyd Group

Author: Daniel Osorio, Daniela Melillo, Ludovic Dickel, Alison G. Cole, Felix Christopher Mark, Livia D'Angelo, Paul L.R. Andrews, Jane A. Smith, Nadav Shashar, Kerry Perkins, Michael J. Kuba, Jennifer A. Basil, Graziano Fiorito, Frank W. Grasso, Andrea Affuso, Giovanna Ponte, Camino Gestal, David D. Smith, Paolo De Girolamo, Stazione Zoologica Anton Dohrn, Association for Cephalopod Research CephRes, Brooklyn College, City University of New-York [New-York] ( CUNY ), Department of Veterinary Medicine and Animal Productions, 'Federico II' University of Naples Medical School, Associazione Italiana per le Scienze degli Animali da Laboratorio ( AISAL ), Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Instituto de Investigaciones Marinas, Consejo Superior de Investigaciones Científicas [Spain] ( CSIC ), BiioMimetic and Cognitive Robotics, Max Planck Institute for Brain Research, Max-Planck-Institut, Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research ( AWI ), University of Sussex, Ben-Gurion University of the Negev ( BGU ), Federation for Laboratory Animal Science Associations ( FELASA ), Boyd Group, St George's University of London, European Cooperation in Science and Technology, Fiorito, Graziano, Affuso, Andrea, Basil, Jennifer, Cole, Alison, DE GIROLAMO, Paolo, D'Angelo, Livia, Dickel, Ludovic, Gestal, Camino, Grasso, Frank, Kuba, Michael, Mark, Felix, Melillo, Daniela, Osorio, Daniel, Perkins, Kerry, Ponte, Giovanna, Shashar, Nadav, Smith, David, Smith, Jane, Andrews, Paul L. R., Stazione Zoologica Anton Dohrn (SZN), Brooklyn College [CUNY, New York], City University of New York [New York] (CUNY), Associazione Italiana per le Scienze degli Animali da Laboratorio (AISAL), Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Brooklyn College [CUNY], City University of New York [New York] (CUNY)-City University of New York [New York] (CUNY), Max-Planck-Gesellschaft, Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung (AWI), Ben-Gurion University of the Negev (BGU), and Federation for Laboratory Animal Science Associations (FELASA)
Subjects: 0106 biological sciences, Research design, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 01 natural sciences, 3R, [ SDV.NEU.SC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Animal welfare, Medicine, Animal Husbandry, media_common, Veterinarian, 0303 health sciences, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, Animal Care Committees, Medicine (all), [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Directive, Research Design, [ SCCO.NEUR ] Cognitive science/Neuroscience, Risk assessment, Inclusion (education), United State, Cephalopods, Consensus, media_common.quotation_subject, invertebrate, Consensu, Veterinarians, Cephalopod, 3Rs, Directive 2010, [ SDV.NEU.PC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, 03 medical and health sciences, media_common.cataloged_instance, Animals, 14. Life underwater, European Union, European union, 030304 developmental biology, Directive 2010/63/EU, Medical education, General Veterinary, business.industry, Animal, 010604 marine biology & hydrobiology, [SCCO.NEUR]Cognitive science/Neuroscience, Animal Care Committee, Invertebrates, United States, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Cephalopoda, Animal Science and Zoology, business, EU, Neuroscience, Welfare
Abstract: 90 pages, This paper is the result of an international initiative and is a first attempt to develop guidelines for the care and welfare of cephalopods (i.e. nautilus, cuttlefish, squid and octopus) following the inclusion of this Class of ∼700 known living invertebrate species in Directive 2010/63/EU. It aims to provide information for investigators, animal care committees, facility managers and animal care staff which will assist in improving both the care given to cephalopods, and the manner in which experimental procedures are carried out. Topics covered include: implications of the Directive for cephalopod research; project application requirements and the authorisation process; the application of the 3Rs principles; the need for harm-benefit assessment and severity classification. Guidelines and species-specific requirements are provided on: i. supply, capture and transport; ii. environmental characteristics and design of facilities (e.g. water quality control, lighting requirements, vibration/noise sensitivity); iii. accommodation and care (including tank design), animal handling, feeding and environmental enrichment; iv. assessment of health and welfare (e.g. monitoring biomarkers, physical and behavioural signs); v. approaches to severity assessment; vi. disease (causes, prevention and treatment); vii. scientific procedures, general anaesthesia and analgesia, methods of humane killing and confirmation of death. Sections covering risk assessment for operators and education and training requirements for carers, researchers and veterinarians are also included. Detailed aspects of care and welfare requirements for the main laboratory species currently used are summarised in Appendices. Knowledge gaps are highlighted to prompt research to enhance the evidence base for future revision of these guidelines., CephRes, AISAL and FELASA support this work. This publication is also supported by COST (European Cooperation in Science and Technology).
Published: 2015
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23. Spatial learning in the cuttlefish Sepia officinalis: preference for vertical over horizontal information

Author: Céline Thomasse, Nadav Shashar, Noam Josef, Gabriella Scatà, Christelle Jozet-Alves, Ben-Gurion University of the Negev (BGU), Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Ben-Gurion University of the Negev ( BGU ), Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), and Normandie Université ( NU ) -Normandie Université ( NU )
Subjects: 0106 biological sciences, Cuttlefish, Cephalopods, cuttlefish, Horizontal and vertical, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Physiology, Hydrostatic pressure, Spatial learning, Aquatic Science, Biology, 010603 evolutionary biology, 01 natural sciences, memory, Cognition, camouflage, 0501 psychology and cognitive sciences, 050102 behavioral science & comparative psychology, Sepia, Set (psychology), Molecular Biology, Sensory cue, Spatial analysis, Ecology, Evolution, Behavior and Systematics, Communication, business.industry, [SCCO.NEUR]Cognitive science/Neuroscience, 05 social sciences, Pattern recognition, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Spatial perception, Insect Science, Camouflage, [ SCCO.NEUR ] Cognitive science/Neuroscience, Animal Science and Zoology, Artificial intelligence, hydrostatic-pressure, business
Abstract: The world is three-dimensional; hence, even surface-bound animals need to learn vertical spatial information. Separate encoding of vertical and horizontal spatial information seems to be the common strategy regardless of the locomotory style of animals. However, a difference seems to exist in the way freely moving species, such as fish, learn and integrate spatial information as opposed to surface-bound species, which prioritize the horizontal dimension and encode it with a higher resolution. Thus, the locomotory style of an animal may shape how spatial information is learned and prioritized. An alternative hypothesis relates the preference for vertical information to the ability to sense hydrostatic pressure, a prominent cue unique to this dimension. Cuttlefish are mostly benthic animals, but they can move freely in a volume. Therefore, they present an optimal model to examine these hypotheses. We tested whether cuttlefish could separately recall the vertical and horizontal components of a learned two-dimensional target, and whether they have a preference for vertical or horizontal information. Sepia officinalis cuttlefish were trained to select one of two visual cues set along a 45 deg diagonal. The animals were then tested with the two visual cues arranged in a horizontal, vertical or opposite 45 deg configuration. We found that cuttlefish use vertical and horizontal spatial cues separately, and that they prefer vertical information to horizontal information. We propose that, as in fish, the availability of hydrostatic pressure, combined with the ecological value of vertical movements, determines the importance of vertical information.
Published: 2015
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24. Rhodnius prolixus smells repellents: Behavioural evidence and test of present and potential compounds inducing repellency in Chagas disease vectors

Author: Paula Zermoglio, Yohan Bignon, Claudio R. Lazzari, Hadrien Martin-Herrou, Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours-Centre National de la Recherche Scientifique (CNRS), Departamento de Ecología, Genética y Evolución [Buenos Aires] (EGE), Facultad de Ciencias Exactas y Naturales [Buenos Aires] (FCEyN), Universidad de Buenos Aires [Buenos Aires] (UBA)-Universidad de Buenos Aires [Buenos Aires] (UBA), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut de recherche sur la biologie de l'insecte ( IRBI ), Université de Tours-Centre National de la Recherche Scientifique ( CNRS ), Departamento de Ecología, Genética y Evolución, IEGEBA (CONICET-UBA), Univ. Buenos Aires, Argentina, Centre de Recherche des Cordeliers ( CRC (UMR_S 872) ), and Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS )
Subjects: 0106 biological sciences, Triatomines, Physiology, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Disease Vectors, 01 natural sciences, Toxicology, DEET, chemistry.chemical_compound, Piperidines, 0303 health sciences, biology, Behavior, Animal, 3. Good health, Smell, Rhodnius, TRIATOMINES, Icaridine, CIENCIAS NATURALES Y EXACTAS, Ir40a+, Chagas disease, Nymph, animal structures, Otras Ciencias Biológicas, Zoology, VECTOR INSECTS, Ciencias Biológicas, 03 medical and health sciences, parasitic diseases, medicine, Animals, Humans, Chagas Disease, Rhodnius prolixus, ICARIDINE, 030304 developmental biology, Vector insects, Host (biology), fungi, Feeding Behavior, biology.organism_classification, medicine.disease, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 010602 entomology, Biting, chemistry, Insect Science, Vector (epidemiology), Insect Repellents, Odorants
Abstract: Insect repellents are known since many decades ago and constitute a major tool for personal protection against the biting of mosquitoes. Despite their wide use, the understanding of why and how repellents repel is relatively recent. In particular, the question about to what extent insects other than mosquitoes are repulsed by repellents remains open. We developed a series of bioassays aimed to test the performance of well established as well as potential repellent molecules on the Chagas disease vector Rhodnius prolixus. Besides testing their ability to prevent biting, we tested the way in which they act, i.e., by obstructing the detection of attractive odours or by themselves. By using three different experimental protocols (host-biting, open-loop orientation to odours and heat-triggered proboscis extension response) we show that DEET repels bugs both in the presence and in the absence of host-associated odours but only at the highest quantities tested. Piperidine was effective with or without a host and icaridine only repelled in the absence of a living host. Three other molecules recently proposed as potential repellents due to their affinity to the Ir40a+ receptor (which is also activated by DEET) did not evoke significant repellency. Our work provides novel experimental tools and sheds light on the mechanism behind repellency in haematophagous bugs. Fil: Zermoglio, Paula Florencia. Centre National de la Recherche Scientifique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Ecología, Genética y Evolución de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Ecología, Genética y Evolución de Buenos Aires; Argentina Fil: Martin Herrou, Hadrien. Centre National de la Recherche Scientifique; Francia Fil: Bignon, Yohan. Centre National de la Recherche Scientifique; Francia Fil: Lazzari, Claudio Ricardo. Centre National de la Recherche Scientifique; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Published: 2015
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25. Time decay of object, place and temporal order memory in a paradigm assessing simultaneously episodic-like memory components in mice

Author: Thomas Freret, Hassina Belblidia, Pascale Schumann-Bard, Abdelmalek Abdelouadoud, Christelle Jozet-Alves, Hélène Dumas, Marianne Leger, Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Université des Sciences et de la Technologie Houari Boumediene [Alger] ( USTHB ), Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), and Université des Sciences et de la Technologie Houari Boumediene [Alger] (USTHB)
Subjects: Male, Time Factors, hippocampus, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SDV]Life Sciences [q-bio], Memory, Episodic, task, Neuropsychological Tests, Spatial memory, 03 medical and health sciences, Behavioral Neuroscience, Mice, 0302 clinical medicine, Explicit memory, Semantic memory, Animals, Episodic-like memory, Visual short-term memory, Episodic memory, 030304 developmental biology, Spatial Memory, 0303 health sciences, Analysis of Variance, Psychological Tests, [ SDV ] Life Sciences [q-bio], Long-term memory, [SCCO.NEUR]Cognitive science/Neuroscience, Sensory memory, Recognition, Psychology, Object recognition, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, rats, NMRI mice, Time Perception, [ SCCO.NEUR ] Cognitive science/Neuroscience, Exploratory Behavior, Psychology, 030217 neurology & neurosurgery, medial prefrontal cortex, Cognitive psychology
Abstract: WOS:000353599600013; International audience; A common trait of numerous memory disorders is the impairment of episodic memory. Episodic memory is a delay-dependant memory, especially associating three components, the "what", "where" and "when" of a unique event. To investigate underlying mechanisms of such memory, several tests, mainly based on object exploration behaviour, have been set up in rodents. Recently, a three-trial object recognition task has been proposed to evaluate simultaneously the different components of episodic-like memory in rodents. However, to date, the time course of each memory component in this paradigm is not known. We characterised here the time course of memory decay in adult mice during the three-trial object recognition task, with inter-trial interval (ITI) ranging from 1 h to 4 h. We found that, with 1 hand 2 h, but not 4h ITI, mice spent more time to explore the displaced "old object" relative to the displaced "recent object", reflecting memory for "what and when". Concomitantly, animals exhibited more exploration time for the displaced "old object" relative to the stationary "old object", reflecting memory for "what and where". These results provide strong evidence that mice establish an integrated memory for unique experience consisting of the "what", "where" and "when" that can persist until 2 h ITI. (C) 2015 Elsevier B.V. All rights reserved.
Published: 2015
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26. Effects of rearing conditions on sand digging efficiency in juvenile cuttlefish

Author: Raymond Chichery, Roseline Poirier, Ludovic Dickel, EA3211 Physiologie du Comportement des Céphalopodes, Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Université de Caen Normandie ( UNICAEN ), and Normandie Université ( NU ) -Normandie Université ( NU )
Subjects: 0106 biological sciences, Cuttlefish, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Environment, Sepia officinalis, 010603 evolutionary biology, 01 natural sciences, Cephalopod, [ SDV.NEU.PC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, Sand digging, [ SDV.NEU.SC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Behavioural development, 03 medical and health sciences, Behavioral Neuroscience, 0302 clinical medicine, Animal science, Animals, Substrate (aquarium), Juvenile, 14. Life underwater, Mollusca, Developmental stage, Behavior, Animal, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, biology, Hatching, Ecology, [SCCO.NEUR]Cognitive science/Neuroscience, Age Factors, Fishes, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, General Medicine, Silicon Dioxide, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Digging, [ SCCO.NEUR ] Cognitive science/Neuroscience, Early experience, Animal Science and Zoology, 030217 neurology & neurosurgery
Abstract: International audience; The effect of environment on the maturation of sand digging behaviour in cuttlefish was studied. Sand digging behaviour of cuttlefish individually reared on sand was daily observed in their rearing tanks (first study). Other cuttlefish were individually reared from hatching to 2 weeks of life in different conditions (Group A, on a sandy substrate and group B, without sand). At days 0, 3, 6, 9, 12 and 15, cuttlefish from Groups A and B were placed in a novel tank, the bottom of which was covered by sand (second study). The first study shows that more and more cuttlefish sand dig in their rearing tank during the first 6 days of life. The second study shows that, confronted with a novel sand bottom, cuttlefish from Group A show shorter latencies of sand digging and they cover more completely than do cuttlefish from Group B. This indicates that the developmental changes in sand digging appear not totally pre-programmed, but at least partially experience-dependent. Presence of sand in rearing tanks may allow cuttlefish to acquire experience of digging to make this behaviour more efficient.
Published: 2004
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27. Genetic split between coastal and continental populations of gypsy moth separated by Dinaric Alps

Author: Milan Pernek, Nikola Lacković, Dimitrios N. Avtzis, Christian Stauffer, Coralie Bertheau, Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), University of Natural Resources and Life Sciences [Wien] ( BOKU ), Université médicale de Vienne, Autriche, Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), and University of Natural Resources and Life Sciences [Wien] (BOKU)
Subjects: Range (biology), [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Population, Biology, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, genetic differentiation, geographic isolation, Lymantria dispar, outbreak dynamics, population expansion, range shift, Genetic variation, parasitic diseases, Genetic variability, education, ComputingMilieux_MISCELLANEOUS, education.field_of_study, Genetic diversity, Ecology, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], fungi, Gypsy moth, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Deciduous, [ SDV.BID.EVO ] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], 13. Climate action, Insect Science, Agronomy and Crop Science, [ SDV.BID.SPT ] Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy
Abstract: The gypsy moth, a polyphagous herbivore species, infests mainly deciduous trees in the northern hemisphere, being invasive in North America. In Croatia, gypsy moth is infesting both continental and coastal forests, with the Dinaric Alps posing a physical migratory barrier between two regions. During outbreaks, caterpillars cause severe damages in both regions, though with different outbreak dynamics, which suggests genetic differences between populations. Representative populations from these two regions were screened by sequencing a region of the mitochondrial COI gene. Ninety-nine sequences resulted in seventeen haplotypes, and analyses revealed a significant genetic differentiation between coastal and continental populations, quite likely attributed to geographic isolation and post-glacial history. This differentiation arises from significantly higher genetic variability in Mediterranean population, indicating their higher adaptability, an intriguing fact in case of possible northward range shift of gypsy moth.
Published: 2015
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28. Spatial memory deficit across aging: current insights of the role of 5-HT7 receptors

Author: Christelle Jozet-Alves, Gregory Beaudet, FranÃ§ois Dauphin, Michel Boulouard, Thomas Freret, Valentine Bouet, Pascale Schumann-Bard, Eleni Paizanis, Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), and Normandie Université (NU)-Normandie Université (NU)
Subjects: [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SDV]Life Sciences [q-bio], Cognitive Neuroscience, double dissociation, Hippocampal formation, recognition memory, in-situ hybridization, Spatial memory, lcsh:RC321-571, Mini Review Article, Behavioral Neuroscience, age-differences, rat-brain, spatial cognition, Cognitive decline, navigational strategies, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, Recognition memory, [ SDV ] Life Sciences [q-bio], Cognitive map, [SCCO.NEUR]Cognitive science/Neuroscience, 5-HT 7 R, aging, Cognition, Spatial cognition, Alzheimer's disease, gene-expression, serotonin, Barnes maze, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Neuropsychology and Physiological Psychology, messenger-rna, barnes maze, [ SCCO.NEUR ] Cognitive science/Neuroscience, caudate-nucleus, 5-ht7r, Psychology, Alzheimer’s disease, Neuroscience
Abstract: WOS:000348100700001; International audience; Elderly persons often face biological, psychological or social changes over time that may cause discomfort or morbidity. While some cognitive domains remain stable over time, others undergo a decline. Spatial navigation is a complex cognitive function essential for independence, safety and quality of life. While egocentric (body centered) navigation is quite preserved during aging, allocentric (externally centered) navigation based on a cognitive map using distant landmarks declines with age. Recent preclinical studies showed that serotonergic 5-HT7 receptors are localized in brain regions associated with allocentric spatial navigation processing. Behavioral assessments with pharmacological or genetic tools have confirmed the role of 5-HT7 receptors in allocentric navigation. Moreover, few data suggested a selective age-related decrease in the expression of 5-HT7 receptors in pivotal brain structures implicated in allocentric navigation such as the hippocampal CA3 region. We aim to provide a short overview of the potential role of 5-HT7 receptors in spatial navigation, and to argue for their interests as therapeutic targets against age-related cognitive decline.
Published: 2015
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29. Combining two-dimensional gel electrophoresis and metabolomic data in support of dry-season survival in the two main species of the malarial mosquito Anopheles gambiae

Author: Hidalgo, K., Mouline, K., Mamai, W., Foucreau, N., Dabiré, K.R., Bouchereau, A., Simard, F., Renault, D., Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Institut de Recherche en Sciences de la Santé (IRSS), CNRST, Institut de Recherche pour le Développement (IRD), Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés (LEHNA), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique, Environnement et Protection des Plantes (IGEPP), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Diversity, ecology, evolution & Adaptation of arthropod vectors (MIVEGEC-DEEVA), Evolution des Systèmes Vectoriels (ESV), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), ANR-08-MIEN-0006,DS3-MAL,Stratégies de survie en saison sèche chez les vecteurs majeurs de paludisme en Afrique(2008), Institut de Recherche pour le Développement (IRD [France-Sud])-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE), Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle ( MIVEGEC ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ), Ecosystèmes, biodiversité, évolution [Rennes] ( ECOBIO ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -INEE-Observatoire des Sciences de l'Univers de Rennes ( OSUR ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Recherche en Sciences de la Santé ( IRSS ), Institut de Recherche pour le Développement ( IRD ), Laboratoire d'Ecologie des Hydrosystèmes Naturels et Anthropisés ( LEHNA ), Institut National de la Recherche Agronomique ( INRA ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État ( ENTPE ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Génétique, Environnement et Protection des Plantes ( IGEPP ), Institut National de la Recherche Agronomique ( INRA ) -Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -AGROCAMPUS OUEST, ANR-08-MIEN-0006,DS3-MAL,Stratégies de survie en saison sèche chez les vecteurs majeurs de paludisme en Afrique ( 2008 ), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), and Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST
Subjects: [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 2D electrophoresis, Amino acid, Metabolomic, Mosquito, Proteomic, Sugar, lcsh:Computer applications to medicine. Medical informatics, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, parasitic diseases, lcsh:R858-859.7, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, lcsh:Science (General), Data Article, lcsh:Q1-390
Abstract: In dry savannahs of West-Africa, the malarial mosquitoes of the Anopheles gambiae sensu stricto complex annually survive the harsh desiccating conditions of the dry season. However, the physiological and biochemical mechanisms underlying how these mosquitoes survive such desiccating conditions are still undefined, and controversial. In this context, we provide the first work examining both proteomic and metabolomic changes in the two molecular forms of A. gambiae s.s (M and S forms) experimentally exposed to the rainy and dry season conditions as they experience in the field. Protein abundances of the mosquitoes were measured using a two-dimensional fluorescence difference gel electrophoresis (2D DIGE) coupled with a matrix-assisted laser desorption/ionisation-time of flight (MALDI-TOF) and tandem mass spectrometry (MS) for protein identification. These assays were conducted by Applied Biomics (http://www.appliedbiomics.com, Applied Biomics, Inc. Hayward, CA, USA), and the mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier http://www.ebi.ac.uk/pride/archive/projects/PXD000294. The metabolomic analysis was conducted using both Acquity UPLC® system (for amino acid identification), and a gas-chromatography-mass spectrometry platform (for sugars identification). Metabolomic fingerprintings were assessed in the University of Rennes 1, UMR CNRS 6553 EcoBio (France). A detailed interpretation of the obtained data can be found in Hidalgo et al. (2014) [1] (Journal of Insect Physiology (2014)).
Published: 2015
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30. The nasus gland: A new gland in soldiers of Angularitermes (Termitidae, Nasutitermitinae)

Author: Alain Robert, Christian Bordereau, Eliana M. Cancello, Jan Šobotník, Joice P. Constantini, Thomas Bourguignon, Tiago F. Carrijo, Barbora Křížková, Czech University of Life Science, University of Sydney, Museu de Zoologia (MZ), Universidade de São Paulo (USP), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), Université de Bourgogne (UB), Projects CIGA No. 20154314 and IGA No. B03/15 (Czech University of Life Sciences, Prague), the São Paulo Research Foundation (FAPESP) (2013/03767-0), and the project grant 2013/20068-9, the National Council for Scientific and Technological Development, Brazil (CNPq) (308227/2013-0 and 562256/2010-5)., Museu de Zoologia ( MZ ), Universidade de São Paulo ( USP ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Recherche Agronomique ( INRA ), Centre National de la Recherche Scientifique ( CNRS ), and Université de Bourgogne ( UB )
Subjects: defence, Exocrine gland, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Zoology, Biology, [ SDV.BA ] Life Sciences [q-bio]/Animal biology, exocrine organ, Exocrine Glands, Microscopy, Electron, Transmission, Single species, stomatognathic system, biology.animal, medicine, Animals, Angularitermes, Secretion, Ecology, Evolution, Behavior and Systematics, termitoidea, Synapomorphy, Cockroach, cephalic gland, [SDV.BA]Life Sciences [q-bio]/Animal biology, General Medicine, Anatomy, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Termitidae, medicine.anatomical_structure, Insect Science, Microscopy, Electron, Scanning, Ultrastructure, isoptera, neotropical region, termite, Developmental Biology
Abstract: Termites have developed many exocrine glands, generally dedicated to defence or communication. Although a few of these glands occur in all termite species, or represent synapomorphies of larger clades, others are morphological innovations of a single species, or a few related species. Here, we describe the nasus gland, a new gland occurring at the base of the nasus of Angularitermes soldiers. The nasus gland is composed of class 1, 2, and 3 secretory cells, a rare combination that is only shared by the sternal and tergal glands of some termites and cockroaches. The ultrastructural observations suggest that the secretion is produced by class 2 and 3 secretory cells, and released mostly by class 3 cells. The base of the nasus has a rough appearance due to numerous pits bearing openings of canals conducting the secretion from class 3 secretory cells to the exterior. We tentatively assign a defensive function to the nasus gland, although further research is needed to confirm this function. Although the gland is described only from species of Angularitermes, other genera of Nasutitermitinae also present a rough nasus base, suggesting the presence of a similar, possibly homologous, gland.
Published: 2015
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31. Efficient PhiC31 integrase-mediated site-specific germline transformation of Anopheles gambiae

Author: Kenneth D. Vernick, Nicolas Puchot, Catherine Bourgouin, Gareth J Lycett, Emilie Pondeville, Amy Lynd, J. M. Meredith, Paul Eggleston, Génétique et Génomique des Insectes vecteurs, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Keele University [Keele], Liverpool School of Tropical Medicine (LSTM), Support to E.P. was from an ANR-07-MIME-O25-01 award to C.B., from the Roux Foundation (Pasteur Institute) and from a UE323173 Anopath award to K.D.V., to C.B. from award no. ANR-10-LABX-62-IBEID, to P.E. from a Wellcome Trust Programme grant (0084582) and the EU FP7 (INFRAVEC), and to G.J.L. from European Commission grant no. 223736 (TransMalariaBloc) and a Biotechnology and Biological Sciences Research Council grant (no. BB/F021933/1)., We are grateful to the Photography Department of Institut Pasteur, especially F. Gardy and J.-M. Panaud for the production of the video for embryo alignment, and to M. Calos for pET11-ΦC31 and original attP and attB constructs. We thank A. James, A. Handler and N. Windbichler for helpful technical discussions., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), European Project: 323173,EC:FP7:ERC,ERC-2012-ADG_20120314,ANOPATH(2013), European Project: 228421,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2008-1,INFRAVEC(2009), European Project: 223736,EC:FP7:HEALTH,FP7-HEALTH-2007-B,TRANSMALARIABLOC(2008), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Liverpool School of Tropical Medicine ( LSTM ), ANR-10-LABX-62-IBEID,IBEID,Laboratoire d'Excellence 'Integrative Biology of Emerging Infectious Diseases' ( 2010 ), European Project : 323173,EC:FP7:ERC,ERC-2012-ADG_20120314,ANOPATH ( 2013 ), European Project : 228421,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2008-1,INFRAVEC ( 2009 ), European Project : 223736,EC:FP7:HEALTH,FP7-HEALTH-2007-B,TRANSMALARIABLOC ( 2008 ), and Centre National de la Recherche Scientifique ( CNRS ) -Institut Pasteur [Paris]
Subjects: MESH : RNA, Messenger, MESH: Integrases, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Anopheles gambiae, MESH : Animals, Genetically Modified, Messenger/metabolism, Genome, Germline, Animals, Genetically Modified, Genetic Engineering/*methods, Plasmid, MESH: Animals, Genetics, [ SDV.SPEE ] Life Sciences [q-bio]/Santé publique et épidémiologie, 3. Good health, Genetically modified organism, Transformation, MESH : Anopheles, Larva, MESH: Genetic Engineering, Genetic Engineering, Functional genomics, [ SDV.MP.PAR ] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Transgene, Genetically Modified, MESH: Transformation, Genetic, Biology, Integrases/*genetics, General Biochemistry, Genetics and Molecular Biology, MESH : Genetic Engineering, MESH: Animals, Genetically Modified, MESH: Anopheles, MESH : Integrases, Transformation, Genetic, Genetic, Anopheles, parasitic diseases, Animals, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, RNA, Messenger, Anopheles/*genetics, MESH: RNA, Messenger, MESH : Larva, Integrases, fungi, Larva/genetics, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Transformation (genetics), RNA, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, MESH : Animals, MESH : Transformation, Genetic, MESH: Larva
Abstract: International audience; Current transgenic methodology developed for mosquitoes has not been applied widely to the major malaria vector Anopheles gambiae, which has proved more difficult to genetically manipulate than other mosquito species and dipteran insects. In this protocol, we describe PhiC31-mediated site-specific integration of transgenes into the genome of A. gambiae. The PhiC31 system has many advantages over 'classical' transposon-mediated germline transformation systems, because it allows integration of large transgenes at specific, characterized genomic locations. Starting from a general protocol, we have optimized steps from embryo collection to co-injection of transgene-containing plasmid and in vitro-produced PhiC31 integrase mRNA. We also provide tips for screening transgenic larvae. The outlined procedure provides robust transformation in A. gambiae, resulting in homozygous transgenic lines in approximately 2-3 months.
Published: 2014
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32. Cryptic and biochemical responses of young cuttlefish Sepia officinalis exposed to environmentally relevant concentrations of fluoxetine

Author: Flavie Bidel, Cécile Bellanger, Ludovic Dickel, Carole Di Poi, Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), and Normandie Université ( NU ) -Normandie Université ( NU )
Subjects: Cuttlefish, Locomotor activity, Monoamines, Camouflage, Cephalopods, Sepia, animal structures, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Health, Toxicology and Mutagenesis, Zoology, Selective Serotonin Reuptake Inhibitors (SSRI), Growth, 010501 environmental sciences, Aquatic Science, Biology, 01 natural sciences, Toxicology, [ SDV.NEU.PC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, 03 medical and health sciences, [ SDV.NEU.SC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, 0302 clinical medicine, Fluoxetine, Animals, 14. Life underwater, 0105 earth and related environmental sciences, Brain Chemistry, Behavior, Animal, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, Hatching, [SCCO.NEUR]Cognitive science/Neuroscience, Dopaminergic, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Environmental Exposure, biology.organism_classification, Cephalopod, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Monoamine neurotransmitter, [ SCCO.NEUR ] Cognitive science/Neuroscience, Antidepressant, Serotonin, Sand digging behavior, 030217 neurology & neurosurgery, Locomotion, Water Pollutants, Chemical
Abstract: International audience; Antidepressants released in the environment have the potential to generate neural disrupting effects in non-target organisms, yet their putative effects on behaviors have never been studied in cephalopod molluscs. This study assessed the impact of the antidepressant fluoxetine (FLX) on the efficiency of cryptic behaviors (body patterns on uniform, checkerboard and sandy substrates), locomotor activity, and brain chemistry in young cuttlefish exposed to environmental concentrations (1 and 100 ng L−1 of FLX) during the perinatal period. Behavioral responses of cuttlefish were monitored at hatching and two weeks later, and brain monoamine contents were quantified at one month of age. FLX significantly altered the camouflage efficiencies on uniform and sandy backgrounds only at the lowest concentration, but not at 100 ng L−1. Hatchlings exposed to 1 ng L−1 of FLX exhibited a duration exposure-dependent decrease in the uniform camouflage. They also showed a significant increase of the frequency of sand digging behaviors which might make them highly visible to predators in nature. When tested again two weeks later, cuttlefish seemed to have recovered and no more behavioral alterations were observed showing a transitory effect of the antidepressant. FLX did not affect the levels of serotonin, norepinephrine and their metabolites; however, it seemed to influence dopaminergic activity between the two FLX-exposed groups. The results show for the time that environmentally realistic concentrations of a single SSRI significantly impair the cryptic performances of newly hatched cuttlefish, and may ultimately reduce their chance for survival.
Published: 2014
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33. Increase of learning abilities and maturation of the vertical lobe complex during postembryonic development in the cuttlefish,Sepia

Author: Ludovic Dickel, Marie-Paule Chichery, Raymond Chichery, EA3211 Physiologie du Comportement des Céphalopodes, Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), and Normandie Université (NU)-Normandie Université (NU)
Subjects: Cuttlefish, cuttlefish, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, education, Learning abilities, memory, [ SDV.NEU.PC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, [ SDV.NEU.SC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, 03 medical and health sciences, Behavioral Neuroscience, Octopus, 0302 clinical medicine, Predatory behavior, Developmental Neuroscience, biology.animal, Developmental and Educational Psychology, medicine, Animals, 0501 psychology and cognitive sciences, 050102 behavioral science & comparative psychology, Sepia, cephalopod, development, learning, Behavior, Animal, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, biology, [SCCO.NEUR]Cognitive science/Neuroscience, 05 social sciences, Fishes, Brain, Retention, Psychology, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Anatomy, biology.organism_classification, Lobe, Cephalopod, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, medicine.anatomical_structure, [ SCCO.NEUR ] Cognitive science/Neuroscience, Visual learning, Neuroscience, vertical lobe complex, 030217 neurology & neurosurgery, Developmental Biology
Abstract: International audience; When shown prawns in a glass tube, cuttlefish quickly learn to inhibit their predatory behavior. By using a visual learning paradigm, we studied training and retention performances of cuttlefish aged from 8 to 90 days. We found an improvement in the acquisition of learning abilities during the first 2 months of life as well as an increase of 24-hr retention performance between 30 and 90 days of age. Using morphometric measurements of different lobes of the central nervous system, we correlated the emergence of these learning abilities with the postembryonic development of related nervous structures. Our results show that only the growth of the superior frontal and vertical lobes appears to be significantly correlated with the improvement of learning and long-term retention performances. Thus, as found in earlier data collected in Octopus, the vertical lobe complex of the cuttlefish seems to be involved in these learning processes.
Published: 2001
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34. Toxicity of tebufenozide, an ecdysteroid agonist, to [i]Blattella germanica[/i] (Blattodea: Blattellidae)

Author: Kilani-Morakchi, S., Badi, A., Aribi, N., Farine, Jean-Pierre, Soltani, N., ProdInra, Archive Ouverte, Laboratoire de Biologie Animale Appliquée [Annaba] (LBAA), Université Badji Mokhtar - Annaba [Annaba] (UBMA), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), National Fund for Scientific Research, CNEPRU, PNR, Laboratoire de Biologie Animale Appliquée [Annaba] ( LBAA ), Université Badji Mokhtar - Annaba [Annaba] ( UBMA ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), and Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS )
Subjects: [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, reproduction, [SDV.TOX] Life Sciences [q-bio]/Toxicology, Blattella germanica, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SDV.TOX]Life Sciences [q-bio]/Toxicology, [ SDV.TOX ] Life Sciences [q-bio]/Toxicology, [SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, toxicity, tebufenozide
Abstract: The toxicity of the non-steroidal ecdysteroid agonist tebufenozide was evaluated topically on adults of the cosmopolitan cockroach Blattella germanica (Blattodea: Blattellidae). The LD50 value was estimated at 402 mu g/g of insect (range, 95% fiducial limits: 293-552) at 15 days after treatment. The effects on reproduction were observed in adult females of cockroaches that survived exposure to a LD25 (146 mu g/g of insect) of tebufenozide. Dissection of treated females showed a clear reduction in both the number of oocytes per paired ovaries and the size of basal oocytes, as compared to controls. In addition, tebufenozide significantly reduced the mating success of pairs and altered the fertility as shown through the reduction of ootheca development and total absence of viable nymphs. Tebufenozide disrupted the German cockroach reproduction by interferring with the homeostasis of the insect hormones.
Published: 2014

35. Cuttlefish preschool or how to learn in the peri-hatching period

Author: Christelle Jozet-Alves, Anne-Sophie Darmaillacq, Cécile Bellanger, Ludovic Dickel, Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Anne-Sophie Darmaillacq, Ludovic Dickel, Jennifer Mather, Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), and ETHOS, UMR6552
Subjects: Cuttlefish, biology, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, Hatching, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Period (gene), [SCCO.NEUR]Cognitive science/Neuroscience, Peri, [SCCO.NEUR] Cognitive science/Neuroscience, [SDV.NEU.PC] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Cognition, biology.organism_classification, Cephalopod, Developmental psychology, Associative learning, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [ SDV.NEU.PC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, [ SDV.NEU.SC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, [ SCCO.NEUR ] Cognitive science/Neuroscience, [SDV.BA.ZI] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Sepia, Psychology, [SDV.NEU.SC] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2014

36. Response to Commentary on 'Are some invertebrates exquisitely sensitive to the human pharmaceutical fluoxetine?'

Author: C. Di Poi, Cécile Bellanger, Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), and Normandie Université ( NU ) -Normandie Université ( NU )
Subjects: 0106 biological sciences, Fluoxetine, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Ecology, 010604 marine biology & hydrobiology, Health, Toxicology and Mutagenesis, [SCCO.NEUR]Cognitive science/Neuroscience, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, 010501 environmental sciences, Aquatic Science, Pharmacology, Biology, 01 natural sciences, Aquatic toxicology, Aquatic organisms, [ SDV.NEU.PC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [ SDV.NEU.SC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, [ SCCO.NEUR ] Cognitive science/Neuroscience, medicine, 14. Life underwater, 0105 earth and related environmental sciences, medicine.drug, Invertebrate
Abstract: International audience; We appreciate the commentary made by Sumpter and Margiotta-Casaluci (in this issue) who are participating in the brainstorming around the effects of antidepressants on non-target aquatic organisms based on two recent articles published in Aquatic Toxicology, our paper Di Poi et al. (2013) and Franzellitti's article (Franzellitti et al., 2013).
Published: 2014
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37. Objective sampling design in a highly heterogeneous landscape - characterizing environmental determinants of malaria vector distribution in French Guiana, in the Amazonian region

Author: Emmanuel Roux, Isabelle Dusfour, Nadine Dessay, Romain Girod, Christine A Romaña, Pascal Gaborit, UMR 228 Espace-Dev, Espace pour le développement, Université des Antilles ( UA ) -Université de Guyane ( UG ) -Université de Montpellier ( UM ) -Université de la Réunion ( UR ) -Université d'Avignon et des Pays de Vaucluse ( UAPV ) -Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Université de Perpignan Via Domitia ( UPVD ) -Institut de Recherche pour le Développement ( IRD ), Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur ( RIIP ), Université Paris Descartes - Paris 5 ( UPD5 ), Unité d'Entomologie Médicale, Réseau International des Instituts Pasteur ( RIIP ) -Réseau International des Instituts Pasteur ( RIIP ), This study was sponsored by the General Delegation of French Overseas Regions (Délégation Générale à l'Outre-Mer) within the framework of the project 'Bioecology of the vectors of malaria in Cacao, French Guiana: towards assessing the exposure risk and improving the vector control'. The study was also supported by OSE-Guyamapá, a cross-border cooperation project funded by the operational Program 'PO-Amazonie' of the European Regional Development Fund in French Guiana., Université de Guyane (UG)-Université des Antilles (UA)-Institut de Recherche pour le Développement (IRD)-Université de Perpignan Via Domitia (UPVD)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM), Réseau International des Instituts Pasteur (RIIP), Université Paris Descartes - Paris 5 (UPD5), Unité d'entomologie médicale, Vectopôle Amazonien Emile Abonnenc [Cayenne, Guyane française], Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut Pasteur de la Guyane, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Université des Antilles (UA)-Université de Guyane (UG)-Université de Montpellier (UM)-Université de La Réunion (UR)-Avignon Université (AU)-Université de Perpignan Via Domitia (UPVD)-Institut de Recherche pour le Développement (IRD), and Institut de Recherche pour le Développement (IRD)-Université de Perpignan Via Domitia (UPVD)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Université de Guyane (UG)-Université des Antilles (UA)
Subjects: Jaccard index, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 030231 tropical medicine, Land cover, 03 medical and health sciences, 0302 clinical medicine, Environmental Science(all), Abundance (ecology), Anopheles, Environmental monitoring, Sampling design, [ INFO.INFO-TI ] Computer Science [cs]/Image Processing, Animals, Cluster Analysis, 030212 general & internal medicine, Ecosystem, Ecology, Evolution, Behavior and Systematics, General Environmental Science, [STAT.AP]Statistics [stat]/Applications [stat.AP], [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, Ecology, [ STAT.AP ] Statistics [stat]/Applications [stat.AP], Sampling (statistics), 15. Life on land, French Guiana, Insect Vectors, Malaria, 3. Good health, Stratified sampling, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Geography, [INFO.INFO-TI]Computer Science [cs]/Image Processing [eess.IV], Remote Sensing Technology, Principal component analysis, Geographic Information Systems, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Environmental Monitoring, Research Article
Abstract: International audience; BACKGROUND: Sampling design is a key issue when establishing species inventories and characterizing habitats within highly heterogeneous landscapes. Sampling efforts in such environments may be constrained and many field studies only rely on subjective and/or qualitative approaches to design collection strategy. The region of Cacao, in French Guiana, provides an excellent study site to understand the presence and abundance of Anopheles mosquitoes, their species dynamics and the transmission risk of malaria across various environments. We propose an objective methodology to define a stratified sampling design. Following thorough environmental characterization, a factorial analysis of mixed groups allows the data to be reduced and non-collinear principal components to be identified while balancing the influences of the different environmental factors. Such components defined new variables which could then be used in a robust k-means clustering procedure. Then, we identified five clusters that corresponded to our sampling strata and selected sampling sites in each stratum. RESULTS: We validated our method by comparing the species overlap of entomological collections from selected sites and the environmental similarities of the same sites. The Morisita index was significantly correlated (Pearson linear correlation) with environmental similarity based on i) the balanced environmental variable groups considered jointly (p = 0.001) and ii) land cover/use (p-value
Published: 2013
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38. Chapter 25. Learning, Memory, and Brain Plasticity in Cuttlefish (Sepia officinalis)

Author: Dickel , Ludovic, Darmaillacq , Anne-Sophie, Jozet-Alves , Christelle, Bellanger , Cécile, Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Randolf Menzel, Paul Benjamin, Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), and Normandie Université ( NU ) -Normandie Université ( NU )
Subjects: [ SDV.NEU.PC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [ SDV.NEU.SC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SCCO.NEUR]Cognitive science/Neuroscience, [ SCCO.NEUR ] Cognitive science/Neuroscience, education, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences
Abstract: International audience; This chapter summarizes the literature on the anatomical and functional organization of the cuttlefish brain, with a focus on the structures involved in learning and memory processes (namely the vertical lobe system and optic lobes). Also, different learning paradigms that are commonly used in Sepia officinalis are described with, when possible, their neural correlates. Recent work on the early development of brain and memory is also reviewed. Some research directions to follow in the field of neurobiology of learning and memory in cuttlefish are suggested to better understand the extraordinary behavioral plasticity of these sophisticated invertebrates.
Published: 2013
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39. Maturation of polarization and luminance contrast sensitivities in cuttlefish (Sepia officinalis)

Author: Anne-Sophie Darmaillacq, Lelia Cartron, Ludovic Dickel, Nadav Shashar, Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Department of Life Sciences, Ben-Gurion University of the Negev ( BGU ), Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), and Ben-Gurion University of the Negev (BGU)
Subjects: 0106 biological sciences, Cuttlefish, Cephalopods, Luminescence, Sepia, Light, Physiology, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, media_common.quotation_subject, Prey detection, Aquatic Science, Biology, 010603 evolutionary biology, 01 natural sciences, Luminance, contrast vision, Contrast Sensitivity, 03 medical and health sciences, 0302 clinical medicine, Optics, prey detection, predatory behavior, Contrast (vision), Animals, optomotor response, 14. Life underwater, Molecular Biology, Ecology, Evolution, Behavior and Systematics, Vision, Ocular, media_common, business.industry, Ecology, [SCCO.NEUR]Cognitive science/Neuroscience, Polarization (waves), biology.organism_classification, Cephalopod, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Insect Science, [ SCCO.NEUR ] Cognitive science/Neuroscience, Optomotor response, Animal Science and Zoology, business, 030217 neurology & neurosurgery
Abstract: SummaryPolarization sensitivity (PS) is a characteristic of the visual system of cephalopods. It has been well documented in adult cuttlefish which use PS in a large range of tasks such as communication, orientation and predation. Since cuttlefish do not benefit from parental care, their visual system and motion detection ability need to be efficient from hatching to enable them to detect prey or predators. We studied the maturation and functionality of polarization sensitivity in newly hatched cuttlefish. In a first experiment, we examined the response of juvenile cuttlefish from hatching to the age of one month, towards a moving, vertically-oriented grating (contrasting and polarized stripes) using an optomotor response apparatus. Cuttlefish showed differences in maturation of polarization vs. intensity contrast motion detection. In a second experiment, we examined the involvement of polarization information in prey preference and detection in cuttlefish of the same age. Cuttlefish chose preferentially not to attack transparent prey in which the polarization contrast has been removed with a depolarizing filter. Performances of prey detection based on intensity contrast improved with age. Polarization contrast can help cuttlefish detect transparent prey. Our results suggest that polarization is not a simple modulation of intensity information, but rather that it is processed as a distinct channel of visual information. Both intensity and polarization sensitivity are functional, though not fully matured, in newly hatched cuttlefish and seem to help in prey detection.
Published: 2013
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40. Feel, smell and see in an egg: emergence of perception and learning in an immature invertebrate, the cuttlefish embryo

Author: Ludovic Dickel, Cécile Bellanger, Anne-Sophie Darmaillacq, Sébastien Romagny, Mathieu Guibé, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), French Ministry of Education and Research (MESR), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), and Normandie Université ( NU ) -Normandie Université ( NU )
Subjects: Cuttlefish, Embryo, Nonmammalian, Sepia, animal structures, cuttlefish, Sensory development, genetic structures, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Physiology, media_common.quotation_subject, Sensory system, Aquatic Science, Biology, Stimulus (physiology), Motor Activity, In ovo, 03 medical and health sciences, 0302 clinical medicine, Rhythm, Memory, Perception, Animals, Learning, [ SDV.BDD ] Life Sciences [q-bio]/Development Biology, Habituation, Mantle (mollusc), Habituation, Psychophysiologic, Molecular Biology, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Ecology, Evolution, Behavior and Systematics, 030304 developmental biology, media_common, 0303 health sciences, Communication, business.industry, [SCCO.NEUR]Cognitive science/Neuroscience, Olfactory Perception, habituation, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Touch Perception, Embryo, Insect Science, [ SCCO.NEUR ] Cognitive science/Neuroscience, embryonic structures, Visual Perception, Animal Science and Zoology, business, Neuroscience, 030217 neurology & neurosurgery
Abstract: SUMMARYIt is now well established that prenatal sensory experience affects development itself and has long-term consequences in terms of postnatal behavior. This study focused on the functionality of the sensory system in cuttlefish in ovo. Embryos of stage 23, 25 and 30 received a tactile, chemical or visual stimulus. An increase of mantle contraction rhythm was taken to indicate a behavioral response to the stimulus. We clearly demonstrated that tactile and chemical systems are functional from stage 23, whereas the visual system is functional only from stage 25. At stage 25 and 30, embryos were also exposed to a repeated light stimulus. Stage 30 embryos were capable of habituation, showing a progressive decrease in contractions across stimulations. This process was not due to fatigue as we observed response recovery after a dishabituation tactile stimulus. This study is the first to show that cuttlefish embryos behaviorally respond to stimuli of different modalities and that the visual system is the last to become functional during embryonic development, as in vertebrate embryos. It also provides new evidence that the memory system develops in ovo in cuttlefish.
Published: 2012
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41. Laboratory selection for an accelerated mosquito sexual development rate

Author: Clelia F. Oliva, Guy Lempérière, Jeremie R. L. Gilles, Mark Q. Benedict, Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle (MIVEGEC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Insect Pest Control Laboratory (IPC laboratory), Food and Agriculture Organization of the United Nations [Rome, Italie] (FAO)-International Atomic Energy Agency [Vienna] (IAEA), Centre de Recherche et de Veille sur les Maladies Émergentes dans l'Océan Indien (CRVOI), Université de La Réunion (UR), Laboratoire de Parasitologie-Mycologie, Montpellier ( MIVEGEC ), Université Montpellier 1 ( UM1 ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Centre Hospitalier Régional Universitaire [Montpellier] ( CHRU Montpellier ) -Institut de recherche pour le développement [IRD] : UR224-Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Insect Pest Control Laboratory, Joint FAO/IAEA Division, International Atomic Energy Agency [Vienna] ( IAEA ), Centre de Recherche et de Veille sur les Maladies Émergentes dans l'Océan Indien ( CRVOI ), and Université de la Réunion ( UR )
Subjects: 0106 biological sciences, Male, lcsh:Arctic medicine. Tropical medicine, [ SDV.BDLR.RS ] Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, lcsh:RC955-962, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Sterility, 030231 tropical medicine, Zoology, Biology, Insemination, 01 natural sciences, lcsh:Infectious and parasitic diseases, [SDV.BDLR.RS]Life Sciences [q-bio]/Reproductive Biology/Sexual reproduction, Sudan, 03 medical and health sciences, Sterile insect technique, 0302 clinical medicine, Spermatheca, Genetic, CULICIDAE, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Anopheles arabiensis, Sexual maturity, Animals, lcsh:RC109-216, Adult stage, Genitalia, Selection, Genetic, Selection, Clinical Laboratory Techniques, Research, Sexual Development, Anatomy, Pupa, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases, 010602 entomology, Infectious Diseases, Parasitology, Female, Entomology
Abstract: Background Separating males and females at the early adult stage did not ensure the virginity of females of Anopheles arabiensis (Dongola laboratory strain), whereas two years earlier this method had been successful. In most mosquito species, newly emerged males and females are not able to mate successfully. For anopheline species, a period of 24 h post-emergence is generally required for the completion of sexual maturation, which in males includes a 180° rotation of the genitalia. In this study, the possibility of an unusually shortened sexual maturity period in the laboratory-reared colony was investigated. Methods The effect of two different sex-separation methods on the virginity of females was tested: females separated as pupae or less than 16 h post-emergence were mated with males subjected to various doses of radiation. T-tests were performed to compare the two sex-separation methods. The rate of genitalia rotation was compared for laboratory-reared and wild males collected as pupae in Dongola, Sudan, and analysed by Z-tests. Spermatheca dissections were performed on females mated with laboratory-reared males to determine their insemination status. Results When the sex-separation was performed when adults were less than 16 h post-emergence, expected sterility was never reached for females mated with radio-sterilized males. Expected sterility was accomplished only when sexes were separated at the pupal stage. Observation of genitalia rotation showed that some males from the laboratory strain Dongola were able to successfully mate only 11 h after emergence and 42% of the males had already completed rotation. A small proportion of the same age females were inseminated. Wild males showed a much slower genitalia rotation rate. At 17 h post-emergence, 96% of the laboratory-reared males had completed genitalia rotation whereas none of the wild males had. Conclusion This colony has been cultured in the laboratory for over one hundred generations, and now has accelerated sexual maturation when compared with the wild strain. This outcome demonstrates the kinds of selection that can be expected during insect colonization and maintenance, particularly when generations are non-overlapping and similar-age males must compete for mates.
Published: 2011
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42. Fatty-acid preference changes during development in Drosophila melanogaster

Author: Jean-Pierre Farine, Anne-Sophie Fougeron, Claude Everaerts, Jean-François Ferveur, Justin Flaven-Pouchon, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), and Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS )
Subjects: [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, lcsh:Medicine, Insect, MESH : Behavior, Animal, Biochemistry, chemistry.chemical_compound, Behavioral Ecology, MESH : Drosophila melanogaster, MESH: Behavior, Animal, MESH: Animals, Palatability, lcsh:Science, MESH : Fatty Acids, media_common, chemistry.chemical_classification, Larva, Multidisciplinary, MESH : Food Preferences, Ecology, Animal Behavior, Behavior, Animal, MESH : Fatty Acids, Unsaturated, Drosophila Melanogaster, Fatty Acids, Age Factors, Animal Models, Neuroethology, MESH: Fatty Acids, Unsaturated, trp, Lipids, Preference, MESH: Fatty Acids, MESH: Dietary Fats, Sex pheromone, dietary fat, Fatty Acids, Unsaturated, taste receptor cell, Sensory Perception, Drosophila melanogaster, Research Article, linoleic acid, media_common.quotation_subject, Linoleic acid, Zoology, larvae, Biology, MESH: Drosophila melanogaster, Food Preferences, Model Organisms, lipid, Animals, MESH: Food Preferences, MESH: Age Factors, Evolutionary Biology, Chemical Ecology, MESH : Larva, lcsh:R, fungi, Fatty acid, biology.organism_classification, Dietary Fats, aggregation pheromone, palatability, metabolism, mutation, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, chemistry, lcsh:Q, MESH : Age Factors, MESH : Animals, MESH: Larva, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, MESH : Dietary Fats, Neuroscience
Abstract: WOS:000296521400044; International audience; Fatty-acids (FAs) are required in the diet of many animals throughout their life. However, the mechanisms involved in the perception of and preferences for dietary saturated and unsaturated FAs (SFAs and UFAs, respectively) remain poorly explored, especially in insects. Using the model species Drosophila melanogaster, we measured the responses of wild-type larvae and adults to pure SFAs (14, 16, and 18 carbons) and UFAs (C18 with 1, 2, or 3 double-bonds). Individual and group behavioral tests revealed different preferences in larvae and adults. Larvae preferred UFAs whereas SFAs tended to induce both a strong aversion and a persistent aggregation behavior. Adults generally preferred SFAs, and laid more eggs and had a longer life span when ingesting these substances as compared to UFAs. Our data suggest that insects can discriminate long-chain dietary FAs. The developmental change in preference shown by this species might reflect functional variation in use of FAs or stage-specific nutritional requirements, and may be fundamental for insect use of these major dietary components.
Published: 2011
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43. A new Eriophyid, Aceria madhucae n. sp. (Acari: Eriophyidae) from India

Author: V. V. Ramamurthy, Sushila Joshi, Pratibha Menon, and Indian Agricultural Research Institute ( IARI )
Subjects: 0106 biological sciences, mites, Arthropoda, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, 010607 zoology, Madhuca longifolia, India, Eriophyidae, Acariformes, 01 natural sciences, Eriophyoidea, Arachnida, Botany, Animalia, Acari, Empodium, Taxonomy, new species, biology, Prostigmata, Seta, Biodiversity, biology.organism_classification, 010602 entomology, Insect Science, Aceria, [ SDV.BID.SPT ] Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy
Abstract: International audience; A new species of Eriophyidae (Acari: Prostigmata: Eriophyoidea), Aceria madhucae n. sp., collected from the lower surface of leaves of Madhuca longifolia (J. Konig) J.F. Macbr. (Sapotaceae), is described from India. Aceria madhucae is distinguished by its 4-rayed empodium, shield with median line present from rear to almost middle of shield, admedian lines, two pair of submedian lines and a pair of short lateral lines present, all parallel to median line and slightly diverging laterally, opisthosomal seta h1 absent, pedipalp genual seta d present.
Published: 2011
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44. Spatial Memory

Author: Healy, Susan D., Jozet-Alves, Christelle, University of St Andrews [Scotland], Groupe Mémoire et Plasticité comportementale ( GMPc ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Michael D. Breed, Janice Moore, Groupe Mémoire et Plasticité comportementale (GMPc), Université de Caen Normandie (UNICAEN), and Normandie Université (NU)-Normandie Université (NU)
Subjects: [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [SCCO.NEUR]Cognitive science/Neuroscience, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Cognitive map, Hippocampus, Navigation, Landmark maze, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [ SDV.NEU.PC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, [ SDV.NEU.SC ] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Sex differences, [ SCCO.NEUR ] Cognitive science/Neuroscience, Beacon
Abstract: International audience; Spatial memory is used to return to rewarding locations (home, foraging sites, and so on) and has been demonstrated in a wide diversity of animals, ranging from the invertebrates through to humans. The particular kinds of information (cues) used are also variable, although visual landmarks, distances, and directions often form the basis for this kind of memory. Almost all tests of spatial memory have been conducted in the laboratory, using mazes of different kinds, relying on food or escape as the motivation for performance. The main brain region involved in processing spatial information (in vertebrates) is the hippocampus, which varies in size in relation to the spatial demands placed on animals by their ecology.
Published: 2010
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45. Evolution et radiations adaptatives chez les échinides

Author: Didier Néraudeau, Bruno David, Rich Mooi, Thomas Saucède, Loïc Villier, Biogéosciences [Dijon] ( BGS ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Géosciences Rennes ( GR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre Armoricain de Recherches en Environnement-Centre National de la Recherche Scientifique ( CNRS ), Géologie des Systèmes Carbonatés ( FRE 2761 ), Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique ( CNRS ), Biogéosciences [UMR 6282] [Dijon] (BGS), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Department of Invertebrate Zoology & Geology, California Academy of Sciences, Géosciences Rennes (GR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre Armoricain de Recherches en Environnement-Centre National de la Recherche Scientifique (CNRS), Géologie des Systèmes Carbonatés (FRE 2761 ), Centre National de la Recherche Scientifique (CNRS)-Université de Provence - Aix-Marseille 1, Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique (CNRS), Biogéosciences [UMR 6282] (BGS), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre Armoricain de Recherches en Environnement-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, 010506 paleontology, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, Radiation, biology, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Échinides, Echinozoa, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], General Engineering, Évolution, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [ SDV.BID.EVO ] Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Mesozoic, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, [SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/Paleontology, Cenozoic, Humanities, Geology, 0105 earth and related environmental sciences, [ SDU.STU.PG ] Sciences of the Universe [physics]/Earth Sciences/Paleontology
Abstract: International audience; Les échinides (oursins) sont apparus au Paléozoïque inférieur et, après une première phase de diversification au Paléozoïque, ont été les acteurs d'une radiation évolutive très intense au cours du Mésozoïque et du Cénozoïque. Sur la base d'une reconsidération des homologies de la paroi du corps des échinodermes, sont présentés quelques aspects majeurs de l'évolution des oursins : l'origine de l'irrégularité et de la symétrie bilatérale ; un modèle de radiation, celle des spatangues ; la diversification des modes de reproduction. Ces exemples permettent de prendre la mesure des capacités d'innovation dont ont fait preuve les oursins au cours de leur radiation postpaléozoïque.
Published: 2009
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46. The genus Teliocrinus (Crinoidea, Echinodermata): a key taxon among pentacrinid stalked crinoids

Author: Marc Eléaume, Michel Roux, Nadia Améziane, Biogéosciences [UMR 6282] [Dijon] (BGS), Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Biologie des organismes marins et écosystèmes (BOME), Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Muséum national d'Histoire naturelle (MNHN), Biogéosciences [Dijon] ( BGS ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Biologie des organismes marins et écosystèmes ( BOME ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), Biogéosciences [UMR 6282] (BGS), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), and Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0106 biological sciences, 010506 paleontology, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Biology, Subspecies, 010603 evolutionary biology, 01 natural sciences, Isocrinida, taxonomy, Genus, evolution, Animalia, Crinoidea, 14. Life underwater, Neoteny, Ecology, Evolution, Behavior and Systematics, 0105 earth and related environmental sciences, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, fossil, Ecology, Pentacrinitidae, Biodiversity, Cretaceous, extant, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Cainocrinidae, Symplesiomorphy, Taxon, ontogeny, Evolutionary biology, Animal Science and Zoology, Taxonomy (biology), [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Isselicrinidae, Heterochrony, Echinodermata
Abstract: 18 pages; International audience; The main characters of the stalked crinoids of the family Pentacrinitidae attributed to the genus Teliocrinus are re-evaluated from a quantitative study of phenotype variation, new observations on arm and stalk articulations, and observation of ontogenetic trends. All of the specimens collected in the northern Indian Ocean belong to the same species, i.e. Teliocrinus springeri (Clark, 1909). However, two phenotypes living at different depths remain valid as subspecies: Teliocrinus springeri springeri (Clark, 1909) and Teliocrinus springeri liliaceus (Clark, 1909). Teliocrinus shares several ontogenetic trends with Endoxocrinus, especially in nonfunctional brachial articulations and stalk symplexies. Its assignment to the Diplocrininae is confirmed. A discussion of its affinities with pentacrinid fossil genera in which the crown is well preserved suggests that Diplocrininae could have first appeared during the Lower Cretaceous. A shortening of brachitaxes and a paedomorphic trend of stalk symplexies are the main other evolutionary traits. Nonfunctional articulations are frequently found at the paedomorphic pole of the heterochronic gradient, without clear derived characters. Classification of pentacrinids mainly based on such symplesiomorphy or paedomorphic characters must be definitively abandoned. However, in post-Palaeozoic stalked crinoids the scarcity of well-preserved fossils, the high frequency of paedomorphy, and convergent adaptive characters makes phylogenetic reconstruction only based on morphological characters very difficult and speculative.
Published: 2009
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47. Microstructures of Antarctic cidaroid spines: diversity of shapes and ectosymbiont attachments

Author: Stuart R. Stock, Vincent Heterier, Bruno David, Francesco De Carlo, Chantal De Ridder, Biogéosciences [UMR 6282] [Dijon] (BGS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Department of Molecular Pharmacology and Biological Chemistry, Northwestern University, Advanced Photon Source [ANL] (APS), Argonne National Laboratory [Lemont] (ANL)-University of Chicago-US Department of Energy, Laboratoire de Biologie marine, Université libre de Bruxelles (ULB), Funding from the BIANZO I and II projects supported by the Belgian Science Policy (PADDII projects), and from a PhD grant from the Belgian Science Policy (Belspo)., ANR-07-BLAN-0213,ANTFLOCKS,ANTARCTIC SHELF AS A SPECIES FLOCKS GENERATOR(2007), Biogéosciences [Dijon] ( BGS ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Advanced Photon Source [ANL] ( APS ), Argonne National Laboratory [Lemont] ( ANL ) -University of Chicago-US Department of Energy, Université Libre de Bruxelles [Bruxelles] ( ULB ), and ANR : ANTFLOCKS,ANTFLOCKS
Subjects: 0106 biological sciences, Stereom, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Aquatic Science, 010603 evolutionary biology, 01 natural sciences, Endoskeleton, Cortex (anatomy), medicine, [ SDV.EE.IEO ] Life Sciences [q-bio]/Ecology, environment/Symbiosis, 14. Life underwater, Ecology, Evolution, Behavior and Systematics, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, Ecology, biology, 010604 marine biology & hydrobiology, Micro computed tomography, Viral tegument, Anatomy, biology.organism_classification, Spine (zoology), [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, medicine.anatomical_structure, Epidermis (zoology), Echinoderm, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, [SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis
Abstract: 14 pages; International audience; The echinoderm endoskeleton, located in the connective layer of the tegument, is organized into a threedimensional mesh, the stereom. Among echinoids, the cidaroids depart from this pattern, and the shaft of the spine lacks an epidermis. Thus, the spines lack antifouling protection, allowing ectosymbionts such as bryozoans and foraminiferans to attach. This raises a question about the adaptive role of the cortical layer of the stereom. This study examined the micro- and mesostructure of the spines of 11 cidaroid species collected in the Weddell Sea and Drake Passage, and the nature of their ectosymbiont attachments. Scanning electron microscopy was used to characterize the cortex surface and X-ray micro computed tomography (µCT) to describe the symbiont attachments. Spine microstructure features provide a useful taxonomic character for distinguishing among three species in the genus Ctenocidaris, and challenge a previous parasitic interpretation of cortical Wlaments on the spines of Rhynchocidaris triplopora. Ectosymbiont attachments were classiWed as Anchoring, Molding, Cementing, or Corroding. The study suggests that some microstructure features may be protective, keeping the ectosymbionts away from the cortex and loosely attached at intervals along the shaft of the spine, while other micro-structures facilitate attachment over considerable areas of the shaft.
Published: 2009
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48. The morphology, ontogeny, and inferred behaviour of the deep-sea echinoid Calymne relicta (Holasteroida)

Author: Bruno David, Rich Mooi, Alexander N. Mironov, Thomas Saucède, Biogéosciences [Dijon] ( BGS ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), P.P. Shirshov Institute of Oceanology ( SIO ), Russian Academy of Sciences [Moscow] ( RAS ), Department of Invertebrate Zoology & Geology, California Academy of Sciences, Study partly supported by a grant of the Russian Foundation for Basic Research No. 05-05-65283., Biogéosciences [UMR 6282] [Dijon] (BGS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, P.P. Shirshov Institute of Oceanology (SIO), and Russian Academy of Sciences [Moscow] (RAS)
Subjects: 0106 biological sciences, Systematics, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Ontogeny, Calymnidae, Zoology, Morphology (biology), spines, [SDV.BID]Life Sciences [q-bio]/Biodiversity, appendages, 010603 evolutionary biology, 01 natural sciences, Deep sea, systematics, Paleontology, Type (biology), Animalia, 14. Life underwater, Ecology, Evolution, Behavior and Systematics, Taxonomy, Holasteroida, Appendage, [ SDV.BID ] Life Sciences [q-bio]/Biodiversity, geography, functional morphology, [ SDE.BE ] Environmental Sciences/Biodiversity and Ecology, geography.geographical_feature_category, biology, Calymne relicta, 010604 marine biology & hydrobiology, Abyssal plain, Echinoidea, Biodiversity, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Animal Science and Zoology, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Echinodermata
Abstract: The deep-sea holasteroid Calymne relicta was first described from a few fragments discovered by the HMS ‘Challenger’ in the Bermuda abyssal plain more than a century ago. In addition to re-examining the type material, we describe herein new specimens from unpublished material collected between 3720 and 4860 m during three scientific expeditions that took place on both sides of the North Atlantic between 1966 and 1991. The new material includes juvenile and adult specimens in sufficiently good preservational state to allow a full redescription, including all types of appendages, some of which have never been described. These new observations confirm the atypical characteristics of C. relicta, including test morphology and spines that have no equivalent in other extant taxa. We explore in detail characteristics of this species that were almost impossible to interpret previously. © 2009 The Linnean Society of London, Zoological Journal of the Linnean Society, 2009, 155, 630–648.
Published: 2009
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49. Antimicrobial defense and persistent infection in insects

Author: Haine , Eleanor R., Moret , Yannick, Siva-Jothy , Michael T., Rolff , Jens, Department of Animal and Plant Sciences [Sheffield], University of Sheffield [Sheffield], Biogéosciences [Dijon] ( BGS ), and Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS )
Subjects: [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology, [ SDV.IMM.IA ] Life Sciences [q-bio]/Immunology/Adaptive immunology
Abstract: 3 pages; International audience; During 400 million years of existence, insects have rarely succumbed to the evolution of microbial resistance against their potent antimicrobial immune defenses. We found that microbial clearance after infection is extremely fast and that induced antimicrobial activity starts to increase only when most of the bacteria (99.5%) have been removed. Our experiments showed that those bacteria that survived exposure to the insect's constitutive immune response were subsequently more resistant to it. These results imply that induced antimicrobial compounds function primarily to protect the insect against the bacteria that persist within their body, rather than to clear microbial infections. These findings suggest that understanding of the management of antimicrobial peptides in natural systems might inform medical treatment strategies that avoid the risk of drug resistance.
Published: 2008
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50. Temporal patterns in immune responses to a range of microbial insults (Tenebrio molitor)

Author: Jens Rolff, Yannick Moret, Michael T. Siva-Jothy, Laura C. Pollitt, Eleanor R. Haine, Department of Animal and Plant Sciences [Sheffield], University of Sheffield [Sheffield], Biogéosciences [Dijon] ( BGS ), Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), and Funded by the Leverhulme Trust and by a Wellcome Trust vacation scholarship.
Subjects: Mealworm, Prophenoloxidase, Time Factors, Physiology, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Antimicrobial peptides, Bacillus subtilis, Microbiology, Immune system, Downregulation and upregulation, Hemolymph, Escherichia coli, Animals, [ SDV.IMM ] Life Sciences [q-bio]/Immunology, Tenebrio, Enzyme Precursors, biology, Monophenol Monooxygenase, Zone of inhibition, Long-lasting immunity, Antimicrobial, biology.organism_classification, Haemolymph, Insect Science, Host-Pathogen Interactions, Insect immunity, Phenoloxidase, Catechol Oxidase, Antimicrobial Cationic Peptides
Abstract: 8 pages; International audience; Much work has elucidated the pathways and mechanisms involved in the production of insect immune effector systems. However, the temporal nature of these responses with respect to different immune insults is less well understood. This study investigated the magnitude and temporal variation in phenoloxidase and antimicrobial activity in the mealworm beetle Tenebrio molitor in response to a number of different synthetic and real immune elicitors. We found that antimicrobial activity in haemolymph increased rapidly during the first 48h after a challenge and was maintained at high levels for at least 14 days. There was no difference in the magnitude of responses to live or dead Escherichia coli or Bacillus subtilis. While peptidoglylcan also elicited a long-lasting antimicrobial response, the response to LPS was short lived. There was no long-lasting upregulation of phenoloxidase activity, suggesting that this immune effector system is not involved in the management of microbial infections over a long time scale.
Published: 2008
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