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Background and Objective: The aim of this study was to evaluate the chemical and microbial quality of bottled water in Iran and to calculate the water quality index (WQI). Materials and Methods: Different brands of bottled water (4 samples from 71 brands) were randomly collected from the market. Chemical and microbial characteristics of the samples were examined and determined. Finally, the calculations related to the WQI index were performed and the water samples were classified as excellent, good, poor, very poor and unsuitable. Results: None of the samples exhibited concentration of heavy metals beyond Iranian water standards, and the concentration of sulfate (SO4), chloride (Cl) and fluoride (F) did not exceed international standards. However, in some samples, nitrite (NO2) and nitrate (NO3) concentrations were higher than recommended standards. With Regard to other water quality parameters, 8% to 89% of the samples exhibited concentration higher than the values provided on the water bottle label. 5 species of different bacteria were found in 15 water samples. According to the WQI index, about 63% of the samples were of excellent quality. Also, the water quality of 34% and 3% of the samples fell in good and poor quality categories, respectively. None of the bottled water samples was of very poor quality. Conclusion: The quality of bottled water investigated in this study was generally suitable, but due to the wide range of bottled water in Iran based on brand and seasons, continuous evaluation of water treatment methods in companies and careful monitoring of chemical and microbial quality of bottled water in all seasons is recommended. [ABSTRACT FROM AUTHOR]