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The peel of Hylocereus undatus was employed in the preparation of biochar and firstly applied for tetracycline removal from aqueous solution. Based on different characterization techniques, the material was found to possess a variety of surface functional groups on a porous structure and a pH point of zero charge (pHpzc) of 9.3. Adsorption of tetracycline (TC) was conducted under varying conditions, revealing significant effects of carbonization temperature, solution pH, adsorbent dose, ionic strength, contact time and initial concentration of TC on the biochar adsorption capacity. Kinetic data on TC adsorption were best described using the Elovich kinetic model, with an initial adsorption rate of 167.3 mg g−1 min−1. Isotherm data on adsorption of the desired biochar showed the best fit with the Temkin isotherm model, followed by the Langmuir model, displaying maximum adsorption capacity at 12.4 mg g−1. The electrostatic interactions between the charged biochar surfaces and certain fractions of TC were proposed as the major mechanism, together with H-bonding, pore-filling effect and π–π interaction. This study demonstrates great potential of H. undatus peel as a starting material to prepare an effective and reusable adsorbent in the removal of TC. NOVELTY STATEMENT: Literature is available on a large number of plant-based biochar adsorbents for the removal of antibiotics. However, to the best of our knowledge, no report has been published on applying biochar derived from Hylocereus undatus peel for antibiotics removal. This type of fruit peel is available in massive amounts in Vietnam and is considered as an agricultural solid waste. Therefore, to fill the gap in the literature and to converse this leftover waste into a value-added byproduct, we chose this study to prepare H. undatus peel-derived biochar for the elimination of TC in an aqueous solution. [ABSTRACT FROM AUTHOR]

The aim of this research is to assess the effectiveness of gamma irradiation in developing genetic variability in dragon fruit by influencing biochemical attributes. Seeds of dragon fruit were exposed to 100, 200, 300, 400 and 500 Gy γ-radiation using Co-60 source. Highest germinability, mean germination time, mean daily germination, vigour index and growth rate were observed in untreated seeds, however these traits were relatively less affected at lower irradiation level (up to 200 Gy). An irradiation level of ≥ 400 Gy severely affected germination traits and seedling growth and consequently 441 Gy irradiation level was considered as the lethal dose (LD50) for dragon fruit. Photosynthetic pigments, protein content and nutrient content were highest in untreated seedlings and moderately high up to 200 Gy. Findings demonstrated that the concentration of biochemical markers for abiotic stress tolerance viz. proline, phenol, flavonoid and antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase), were significantly high at 200 Gy irradiation level. Hence an irradiation dose of 200 Gy may be considered as an optimal for induced mutagenesis for abiotic stress tolerance in dragon fruit with the least possible unintended damage to seedling growth traits. The findings provide valuable insights into the efficient utilization of γ-irradiation in expediting the development of abiotic stress-tolerant mutant lines of dragon fruit. [ABSTRACT FROM AUTHOR]

This study is aimed at analyzing, evaluating, and optimizing the pectin hydrolysis process to improve the quality of carbonated beverages made from red dragon fruit combined with mint flavor. Red dragon fruit, rich in nutrients and antioxidants, presents challenges in beverage processing due to its high pectin content, which affects clarity and anthocyanin stability. The research investigates the impact of various parameters, including enzyme concentration, hydrolysis time, and temperature, on juice clarity and anthocyanin content. Using a central composite design model, optimal conditions were determined to maximize juice clarity and anthocyanin content. Sensory evaluation during the blending process was also conducted to assess consumer acceptance, focusing on clarity, color, flavor, taste, and total acceptability. The optimum conditions were found to yield maximum clarity (52.763%) and anthocyanin content (224.414 mg C3G/L). Sensory evaluation indicated that 20% dragon fruit juice concentration with 0.12% citric acid, 13°Bx, and 0.30% mint flavor provided the best consumer acceptance. This research demonstrates the potential for producing high‐quality, appealing, and nutritious carbonated beverages from red dragon fruit, contributing to healthier and more sustainable consumption trends. [ABSTRACT FROM AUTHOR]

In this study, ultrasound waves were successfully applied to the osmosis process of dried dragon fruit products. Additionally, this study was aimed at determining the suitable parameters for the process of drying dragon fruit peels. The parameters including the size of slices (2–5 cm), blanching time (10–25 min), ultrasonic time (10–25 min), ultrasonic temperature (45°C–60°C), ultrasonic power (100–250 W), and drying temperature (45°C–60°C) were fully investigated. The parameters including size of slices at 4 cm, blanching time of 20 min at 100°C, ultrasonic time of 15 minutes, ultrasonic temperature of 55°C, ultrasonic power of 100 W, and drying temperature of 55°C displayed the highest vitamin C (22.291 mg acid ascorbic/100 g), total polyphenol content (1096.948 mg GAE/100 g), reducing sugar (40.643 g/L), and total sugar (724.089 g/L). The obtained products were pink, soft, as well as harmonious between sweet and sour taste. This research contributes to diversifying products from dragon fruit in Vietnam. [ABSTRACT FROM AUTHOR]

Background: Cancer is one of the leading causes of death worldwide, with breast and cervical cancers being the most common among women. Over 100,000 new cases of breast cancer and 510,000 new cases of cervical cancer are diagnosed annually. This study aimed to develop and evaluate an eco-friendly, low-cost method to synthesize silver nanoparticles using Hylocereus undatus (dragon fruit) peel extract for their anticancer activity. Results: Silver nanoparticles loaded with Hylocereus undatus fruit peel extract were successfully developed by a green synthesis technique and were optimized by UV–vis spectroscopy. The nanoparticles had an average size of 71.66 nm, a polydispersity index of 0.3754, and a zeta potential of − 38.52, with a spherical shape and 79.5% silver content. Their maximum absorbance was at 448 nm. Further, in vitro anticancer activity via MTT (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide) assay was evaluated and the synthesized nanoparticles displayed IC50 values at 23.51 µg/ml and 23.66 µg/ml against Hela and MDA MB 231 cell lines, respectively. Cytocompatibility studies showed high cell viability (≥ 95%) in L929 mouse fibroblast cells, indicating low toxicity. In silico analyses, including network pharmacology and molecular docking, identified kaempferol and quercetin as key anticancer compounds, with epidermal growth factor receptor (EGFR) (PDB ID: IM17) being the most significant protein target. Docking studies performed by using the Glide module of Schrodinger's software displayed that kaempferol and quercetin had higher binding affinities for EGFR as compared to the standard drug erlotinib, with MET 769 being a crucial binding site. Conclusion: Thus, the outcomes suggest that synthesized silver nanoparticles loaded with Hylocereus undatus fruit peel extract could be a potential and promising drug carrier aiding in cancer treatment. [ABSTRACT FROM AUTHOR]

Featured Application: The proposed new form of smoothie can find an application among young, busy people worldwide who are interested in a nutritious and easy-to-prepare meal, as well as manufacturers of food supplements and food concentrates. The European market offers a variety of functional foods targeted at prediabetic and diabetic patients, with different approaches to reducing postprandial glucose levels. The utilization of white and black mulberry in new products could be an area of interest to many conscious consumers. The study aimed to design a novel form (dried) of easy-to-prepare product by applying nutritious mulberry in various proportions and creating an aesthetically appealing smoothie product with a distinctive taste profile. Such an easy-to-prepare form of smoothie was obtained by utilizing the freeze-drying process, while typical market smoothies are liquid. Sensory evaluations of the fresh, dried, and reconstructed smoothies and their basic characteristics were conducted. Moreover, antiradical tests (with ABTS+• and DPPH•), the phenolic compound profile (HPLC/PDA), and the L*a*b profile were analyzed. The results of the sensory evaluation tests were divided into two groups: Polish and foreign panelists. It was observed that mulberry-licorice drop-based smoothies were preferred fresh. Yet dried and reconstituted capsule-based smoothies were favored overall. Generally, both Polish and foreign panelists preferred lower concentrations of mulberry and licorice extracts (DB) in fresh smoothies, while in dried or reconstructed smoothie form, panelists scored higher (DC) and lower (DB) concentrations quite similarly (favored both concentrations). From the group of identified phenolic compounds, cinnamic acid derivates dominated all analyzed smoothies. Many analyzed types of flavonoids (quercetin, rutin, kaempferol) and naringenin were found in the smoothies. Some differences were noted in the antiradical tests but were only statistically significant for DPPH• scavenging activity, which was higher for the DB1, DB2, and DC1 samples. Generally, even higher addition of licorice and mulberry did not negatively affect panelists' ratings and the analyzed activities. [ABSTRACT FROM AUTHOR]

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AbstractThe present study aimed to optimize the microwave-assisted extraction process for both the peel and pulp of Hylocereus undatus (white dragon fruit) cultivated in Tunisia, using response surface methodology. Total phenolic content, total flavonoid content, FRAP (ferric reducing antioxidant power), and DPPH (1,1-diphenyl-2-picrylhydrazyl) antioxidant activities were optimized. A central composite design (CCD) was applied, considering three key variables: extraction time, extraction temperature, and liquid-to-solid ratio. The optimized extraction parameters for Hylocereus undatus peel and pulp were determined respectively (9.57 min, 42.20 °C and 27.79 mL/g) and (10.08 min, 40.84 °C and 31.52 mL/g). The main phenolic compounds identified in Hylocereus undatus peel and pulp extracts using HPLC-DAD were chlorogenic and caffeic acids and rutin, quercetin, luteolin-7-O-glucoside as flavonoids. Therefore, this research has revealed the potential of a sustainable and eco-friendly process hold promise a directional option and encouraging a circular economy approach for industrial production of antioxidant-rich Hylocereus undatus extracts. [ABSTRACT FROM AUTHOR]

This study aimed to determine the biofunctional properties, such as antioxidant, antimicrobial, anticancer, and antidiabetic activities, of peel extracts obtained through microwave‐assisted extraction (MAE) of red beet, dragon fruit, and prickly pear peels using water as a green solvent. Results indicated that the peel extracts exhibited high total phenolic content (TPC), ranging from 345.93 to 1651.17 mg GAE/L. The DPPH scavenging capacity of the extracts ranged between 7.16 and 34.65 mg/mL, while the ABTS scavenging capacity ranged between 0.93 and 15.27 mg/mL. Dragon fruit peel extract (DFE) and prickly pear peel extract (PPE) showed significant α‐glucosidase inhibition effects, with 67.79% and 37.50% inhibitions, respectively. Moreover, significant antibacterial activities were observed against five pathogenic bacterial strains (B. cereus, E. coli, S. aureus, L. monocytogenes, and S. enterica) at various concentrations of extracts. The cytotoxic effect of the extracts on HT‐29 and HeLa cancer cells was also observed. The most abundant phenolic compound in DFE was rutin (0.558 mg/g); in PPE, hesperidin was the most abundant (0.596 mg/g); and in red beet peel extract (RBE), hesperidin (0.426 mg/g) was the predominant compound. [ABSTRACT FROM AUTHOR]

A flexible, ultrasensitive, and practical SERS chip is presented based on a paper/f-TiO2/Ag structure. The chip enhances the self-assembly of Ag nanoparticles on a cellulose fiber matrix, facilitated by smart functionalized TiO2 nanomaterials (f-TiO2). This design enables superior detection of the hazardous pesticide tricyclazole (TCZ) on crops using an advanced, simple, and efficient analytical method. Despite its straightforward fabrication process via a solvent immersion method, the intrinsic smart surface properties of the TiO2 bridging material - both hydrophilic and hydrophobic - enable the uniform and dense self-assembly of hydrophilic Ag nanoparticles (NPs) on the cellulose fiber paper substrate. This innovative design provides superior sensing efficiency for TCZ molecules with a detection limit reaching 2.1 × 10−9 M, a remarkable improvement compared to Paper/Ag substrates lacking f-TiO2 nanomaterials, which register at 10−5 M. This flexible SERS substrate also exhibits very high reliability as indicated by its excellent reproducibility and repeatability with relative standard deviations (RSD) of only 5.93% and 4.73%, respectively. Characterized by flexibility and a water-attractive yet non-soluble surface, the flexible Paper/f-TiO2/Ag chips offer the convenience of direct immersion into the analytical sample, facilitating seamless target molecule collection while circumventing interference signals. Termed the "dip and dry" technique, its advantages in field analysis are indisputable, boasting in situ deployment, simplicity, and high efficiency, while minimizing interference signals to negligible levels. Through the application of this advanced technique, we have successfully detected TCZ in two high-value crops, ST25 rice and dragon fruit, achieving excellent recovery values ranging from 90 to 128%. This underscores its immense potential in ensuring food quality and safety. As a proof of concept, flexible Paper/f-TiO2/Ag SERS chips, with a simple fabrication process, advanced analytical technique, and superior sensing efficiency, bring SERS one step closer to field applications beyond the laboratory. [ABSTRACT FROM AUTHOR]

This paper provides an overview of the research carried out over the last 25 years on the FAO56 single and basal crop coefficients of subtropical and tropical orchards and plantations of cactus pear, dragon fruit, fig, jujube, passion fruit, pomegranate, cape gooseberry, cherimoya, guava, longan, lychee, mango, papaya, acerola, carambola, cashew, cacao, coffee, jaboticaba, jatropha, macadamia, açai palm, coconut, date palm, guayule, oil palm, peach palm, ramie and rubber tree. The main objective of this review is to update standard single crop coefficients (Kc) and basal crop coefficients (Kcb) and complete the Kc and Kcb values tabulated in FAO56. Kc is the ratio between the non-stressed crop evapotranspiration (ETc) and the grass reference evapotranspiration (ETo), and Kcb is the ratio between the crop transpiration (Tc) and the ETo. When selecting and analysing the literature, only studies that used the FAO Penman–Monteith equation, or another equation well related to the former to compute ETo were considered, while ETc or Tc were obtained from accurate field measurements on crops under pristine (non-stress cropping conditions) or eustress ("good stress") conditions. Articles meeting these conditions were selected to provide data for updating Kc and Kcb under standard conditions. The related description of orchards and plantations refers to crop cultivar and rootstock, irrigation systems and scheduling, planting spacing, fraction of ground cover (fc) by the crops, crop height (h), crop age and training systems, as Kc and Kcb values depend on these characteristics. To define the standard Kc and Kcb values of the selected crops, the values collected in the literature were compared with previously tabulated standard Kc and Kcb values. The updated tabulated values are transferable to other locations and climates and can be used to calculate and model crop water requirements, primarily for irrigation planning and scheduling, and thereby supporting of improved water use and savings, which is the overall aim of the current review. [ABSTRACT FROM AUTHOR]

Due to the complex growth positions of dragon fruit and the difficulty in robotic picking, this paper proposes a six degrees of freedom dragon fruit picking robot and investigates the manipulator's motion characteristics to address the adaptive motion issues of the picking manipulator. Based on the agronomic characteristics of dragon fruit cultivation, the structural design of the robot and the dimensions of its manipulator were determined. A kinematic model of the dragon fruit picking robot based on screw theory was established, and the workspace of the manipulator was analyzed using the Monte Carlo method. Furthermore, a dynamic model of the manipulator based on the Kane equation was constructed. Performance experiments under trajectory and non-trajectory planning showed that trajectory planning significantly reduced power consumption and peak torque. Specifically, Joint 3's power consumption decreased by 62.28%, and during the picking, placing, and resetting stages, the peak torque of Joint 4 under trajectory planning was 10.14 N·m, 12.57 N·m, and 16.85 N·m, respectively, compared to 12.31 N·m, 15.69 N·m, and 22.13 N·m under non-trajectory planning. This indicated that the manipulator operates with less impact and smoother motion under trajectory planning. Comparing the dynamic model simulation and actual testing, the maximum absolute error in the joint torques was −2.76 N·m, verifying the correctness of the dynamic equations. Through field picking experiments, it was verified that the machine's picking success rate was 66.25%, with an average picking time of 42.4 s per dragon fruit. The manipulator operated smoothly during each picking process. In the study, the dragon fruit picking manipulator exhibited good stability, providing the theoretical foundation and technical support for intelligent dragon fruit picking. [ABSTRACT FROM AUTHOR]

Pitahaya (Hylocereus spp.) is an economically significant cactus fruit in Peru, renowned for its rich nutritional profile and antioxidant properties while exhibiting wide biological diversity. This study aimed to morphologically characterize seven pitahaya accessions using qualitative and quantitative descriptors related to the cladodes, flowers, and fruits. Univariate and multivariate (FAMD, PCA, MCA, and clustering) analyses were employed to identify and classify the accessions based on their morphological traits. The analyses revealed three distinct groups: one consisting solely of AC.07; another with AC.02, AC.04, and AC.06; and a third including AC.01, AC.03, and AC.05. The first group exhibited superior characteristics, particularly in fruit traits such as the stigma lobe count (23.3), number of bracts (26.5 mm), and length of apical bracts (15.75 mm). The second group recorded the highest spine count (3.21), bract length (16.95 mm), and awn thickness (5.12 mm). The third group had the highest bract count (37) and an average locule number (23.65). These findings highlight the significant morphological diversity among the accessions, indicating the potential for classification and selection in pitahaya cultivation. The potential of AC.07 stands out in terms of its agronomic qualities, such as its fruit weight (451.93 g) and pulp weight (292.5 g), surpassing the other accessions. [ABSTRACT FROM AUTHOR]

Objectives: Antibiotic-resistant bacterial infections are a growing global concern. A natural remedy for bacterial infections could be available in the Selenicereus undatus fruit, but its antibacterial and biochemical properties are not fully known. Methods: In this study, the biochemical composition and antibacterial, antioxidant, and cytotoxic activities of the Jindu No. 1 (JD) and Bird's Nest (YW) dragon fruit varieties and their potential effects against E. coli, Pseudomonas sp., and Staphylococcus sp. were scrutinized. Results: The JD fruit extract showed higher antibacterial activity than the YW variety against E. coli, Pseudomonas sp., and Staphylococcus sp. in vitro. Additionally, the JD variety demonstrated more significant antioxidant activity than the YW variety and showed less cytotoxic activity. The JD variety had a higher glucose content, while the YW variety had a higher fructose content, and the phytoconstituents analysis confirmed 659 metabolites in total from the two varieties. Through in silico analyses, phytoconstituents were evaluated to identify potential drug molecules against the selected bacterial strain. Moreover, the molecular docking study revealed that riboprobe and Z-Gly-Pro might be effective against E. coli, 4-hydroxy retinoic acid, and that succinyl adenosine may target Pseudomonas sp., and xanthosine and 2′-deoxyinosine-5′-monophosphate may be effective against Staphylococcus sp. These results were further validated by 100 ns Molecular Dynamics (MD) simulation, and all of the selected compounds exhibited acceptable ADMET features. Conclusions: Therefore, phytoconstituents from S. undatus fruit varieties could be employed to fight human bacterial diseases, and future studies will support the continuation of other biological activities in medical research. [ABSTRACT FROM AUTHOR]

Background: Crassulacean acid metabolism plants, such as Stenocereus spp., are climate warming-resilient crops used as food and for by-products elaboration in arid and semi-arid agroecosystems. A few studies on secondary metabolites have been conducted in pitayo fruit (PF), but there are no reports of these compounds in juice powders (JP) with (JPS) or without seeds (JPWS). This study was devoted to characterizing the juice powders (JPS and JPWS) of five pitayas with different flesh colors with regard to some phytochemical and functional attributes. Methods: The study was conducted with a completely random design with factorial arrangement in treatments (PF × JP). Results: Differences among pitayas were related to peel and flesh color attributes. Except for soluble dietary fiber, the remainder of the non-digestible carbohydrates were greater in JPS than in JPWS of all pitayas. Phenols and flavonoids were found to be the highest in the JPWS of all pitayas, whereas total saponins were the highest in JPS of the 'Pink' pitaya. The JPWS of the 'Yellow' and 'Reddish-Purple' pitayas had the highest content of betaxanthins and indicaxanthins, respectively. Antioxidant capacity was the highest in JPS of 'Reddish-Purple' and 'Pink' pitayas. Conclusions: Except for some phenolic compounds, the study suggests that pitayas' JPS would benefit human health when freshly consumed or as elaborated by-products. [ABSTRACT FROM AUTHOR]

Objective: This integrative review aimed to identify the phenolic compounds present in pitayas (dragon fruit). Methods: We employed a comprehensive search strategy, encompassing full-text articles published between 2013 and 2023 in Portuguese, English, and Spanish from databases indexed in ScienceDirect, Capes Periodics, Scielo, and PubMed. The study's selection was guided by the question, "What are the main phenolic compounds found in pitaya fruits?". Results: After screening 601 papers, 57 met the inclusion criteria. The identified phytochemicals have been associated with a range of health benefits, including antioxidant, anti-inflammatory, and anxiolytic properties. Additionally, they exhibit promising applications in the management of cancer, diabetes, and obesity. These 57 studies encompassed various genera, including Hylocereus, Selenicereus, and Stenocereus. Notably, Hylocereus undatus and Hylocereus polyrhizus emerged as the most extensively characterized species regarding polyphenol content. Analysis revealed that flavonoids, particularly kaempferol and rutin, were the predominant phenolic class within the pulp and peel of these fruits. Additionally, hydroxycinnamic and benzoic acid derivatives, especially chlorogenic acid, caffeic, protocatechuic, synaptic, and ellagic acid, were frequently reported. Furthermore, betalains, specifically betacyanins, were identified, contributing to the characteristic purplish-red color of the pitaya peel and pulp. These betalains hold significant potential as natural colorants in the food industry. Conclusion: Therefore, the different pitayas have promising sources for the extraction of pigments for incorporation in the food industry. We recommend further studies investigate their potential as nutraceuticals. [ABSTRACT FROM AUTHOR]

• The red color of the dragon fruit pulp and peel is due to the accumulation of betacyanins. • The betacyanin accumulated calli were encapsulated using sodium alginate and calcium chloride. • The synseeds were cold stored for 6 months at 4.0 °C temperature. • The synseeds were germinated and calli formed on nutrient media. • The betacyanin-loaded synseeds could be stored, transported, and directly used as inoculum for cell cultures to produce betacyanin. The red color of dragon fruit pulp and peel is due to the accumulation of betacyanins. In the present study, callus cultures were established using in vitro germinated seedlings of the white dragon fruit plant (Selenicereus undatus). Murashige and Skoog (MS) medium with 6-benzylaminopurine (BAP) at 2.0 mg/L was used to induce red-colored friable callus (92.0 % response). The betacyanin accumulated calli were harvested and encapsulated using sodium alginate (NaC 6 H 7 O 6) and calcium chloride (CaCl 2). These synseeds were cold stored for 6 months at 4.0 °C temperature. The stored synseeds were germinated on 2.0 mg/L BAP in the medium. The highest germination rate (88 %) of synseeds was achieved with 3 % sodium alginate polymerized with 75 mM calcium chloride, resulting in firm, uniform, and round synseeds. The betacyanin-loaded synseeds could be stored, transported, and directly used as inoculums for the extensive proliferation of calli and cell cultures for the commercial production of betacyanin. [ABSTRACT FROM AUTHOR]

Dragon fruit (Hylocereus spp.), renowned for its aesthetic appeal and rich antioxidant content, has gained global popularity due to its numerous health benefits. In Australia, despite growing commercial interest in cultivating dragon fruit, there is uncertainty for local growers stemming from competition with imported varieties. Notably, there is a lack of comparative research on the shelf-life, antioxidant activity, and phytochemical contents of Australian-grown versus imported dragon fruit, which is crucial for enhancing market competitiveness and consumer perception. This study compares the shelf-life, antioxidant activity, and phytochemical content of Australian-grown and imported dragon fruits under ambient conditions, addressing the competitive challenges faced by local growers. Freshly harvested white-flesh (Hylocereus undatus) and red-flesh (H. polyrhizus) dragon fruit were sourced from Queensland and the Northern Territory and imported fruit were sourced from an importer in Queensland. All fruit were assessed for key quality parameters including peel color, firmness, weight loss, total soluble solids (TSS), pH, titratable acidity (TA), total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), total betalain content (TBC), and total anthocyanin content (TAC). The results indicate that Australian-grown white dragon fruits exhibited average one day longer shelf-life with less color degradation, better firmness retention, and less decline in weight loss, TSS, and acidity compared to imported fruits. Australian-grown red dragon fruits showed similar shelf-life compared to fruits from overseas. Antioxidant activities and phytochemicals were consistently higher in Australian-grown fruits throughout their shelf-life. These findings indicate that Australian-grown dragon fruits offer better physical quality and retain more nutritional value, which could enhance their marketability. [ABSTRACT FROM AUTHOR]

In Nepal, dragon fruit farming is promising but faces limited operation due to its high initial cost during the establishment years. However, its market demand and export potential could provide an economic opportunity for farmers. Here, dragon fruit farmers were categorized as 39 small-scale and 25 large-scale farmers based on an average farm size of 20 kattha (6772.6 m2). A total of 64 dragon fruit farmers were selected randomly from different clusters through a multi-stage sampling technique. The results revealed that the average cost of dragon fruit production per kattha during the establishment year was NRs 259098.58 (1941.89 USD). The gross benefit was NRs 540972.00 (4054.98 USD) in a large-scale farm and NRs 402928.33 (3019.87 USD) in a small-scale farm. Also, an average BC ratio of 1.87 indicated a profitable farming business. Provision of extension services and cooperative engagement were significant among socioeconomic characteristics for the farm category. Land preparation, manure, labor, fertilizers, and plant protection costs were substantial parts of the total cost incurred in dragon fruit cultivation. Pillar and plant protection costs were positively significant in the production function analysis, and the cost of irrigation was negatively significant to gross returns. These findings will encourage the adoption of dragon fruit farming by providing farmers with essential information on production costs and profitability. Market stability, training, extension, and subsidies should be top priorities in authoritative management. [ABSTRACT FROM AUTHOR]

This study examines the use of dragon fruit peel (DFP) powder as a dietary supplement on growth performance, immune responses, and gene expression of Nile tilapia, Oreochromis niloticus, cultured within biofloc systems. A total of 300 Nile tilapia fingerlings (14.64 ± 0.09 g) were subjected to five dietary treatments, with DFP added to a basal diet at doses of 0, 20, 40, 80, and 160 g kg−1 DFP. Growth and immunological responses were assessed after 4 and 8 weeks of feeding, and the transcriptional level of immune and antioxidant‐related genes was measured after 8 weeks. Fish fed diets containing DFP exhibited significantly greater weight gain, faster growth, and enhanced levels of key indicators of immunity than control fish (p < 0.05). A diet containing 40 g DFP kg−1 produced the best result in terms of growth, enhanced immune response indicators in skin mucus and blood serum, and the upregulation of IL‐1, IL‐8, LBP, GPx, GST‐α, and GSR expression (p < 0.05). Based on the quadratic regression analysis, the optimum concentration of DFP was 80 g kg−1. These findings collectively suggest that powdered DFP may serve as a promising feed supplement for Nile tilapia raised in biofloc systems. [ABSTRACT FROM AUTHOR]

Near infrared (NIR) phosphors have been booming as promising candidates for agriculture due to the adjustable spectrum and excellent match with the dyes of the plants. Here, Mn4+ activated double perovskite LaMg 2/3 Mn 1/3 O 3 NIR phosphor was prepared, which showed high match with the absorption spectra of phytochrome P FR. Concentration of Mg2+ was decreased to create octahedron vacancy into the lattice to improve the luminescence performance. Structure and luminescence variation of the phosphors LaMg 2/3- x Nb 1/3 O 3 :Mn4+ along with the increased vacancy concentration were carried out. Relative sensitivity S r of the optimized LaMg 0.57 Nb 0.33 O 3 :Mn4+ reaches 3.109 % and exhibits great application potential in temperature sensor. Both the NIR light emitting diode (LED) and white LED were fabricated by using this double perovskite NIR phosphor and applied to the cultivation of dragon fruit as light compensation. The dragon fruits matured faster than those without any light compensation. These results confirm the great application potential of this double perovskite NIR phosphor in temperature sensor and plant cultivation. [ABSTRACT FROM AUTHOR]

Neoscytalidium dimidiatum is an asexual, opportunistic and necrotrophic pathogen that causes the devastating stem canker disease of dragon fruit species (Selenicereus spp.). Despite this, information about its intraspecific diversity remains lacking. Examination of the morphological variations of 40 N. dimidiatum isolates hosted by S. megalanthus, S. monacanthus and S. undatus from eight allopatric regions in the Philippines detected three distinct morphotypes. Sequencing of three partial gene markers (ITS, ACT and TUB2) of representative isolates revealed low intraspecific genetic diversity of the pathogen from the three hosts. Phylogenetic analysis of internal transcribed spacer (ITS) sequences of the isolates in this study and worldwide collections of the phytopathogen in the GenBank database infers similar founding populations and the possible specificity of the dragon fruit‐infecting N. dimidiatum. Pathogenicity and aggressiveness assay of the isolates on detached stems of dragon fruit did not detect host specificity to S. monacanthus and S. undatus. However, some isolates (i.g., MBDF0802A, MBDF0808B, MBDF0339A and MBDF0007A) significantly had shorter latent period to the stems of S. undatus than S. monacanthus. A higher aggressiveness component of lesion size development on S. undatus stems by isolates from S. monacanthus was recorded suggesting a possible directional selection of highly aggressive N. dimidiatum in this host. This is the first population study on the phenotypic traits and the genetic structure of N. dimidiatum. Valuable inputs for further study of the pathogen are provided, which may help inform the design of management options for stem canker disease of dragon fruit. [ABSTRACT FROM AUTHOR]

In our study, the biological activity of Hylocereus polyrhizus obtained from Türkiye was determined. Firstly, antimicrobial activity of methanol extracts obtained from H. polyrhizus against test microorganisms was determined by disc diffusion susceptibility assay, minimum inhibition concentration (MIC) and minimum fungicidal or bactericidal concentration (MFC or MBC) tests. Then, viable cell counting against Limosilactobacillus fermentum MA-7 using the macro-dilution method was used to determine the probiotic-promoting effect of the fruit extracts. Finally, the photo-protective activity of the extracts and extract-cream mixtures was obtained spectrophotometrically. The inhibition zone diameter of the peel extract against the test microorganisms is in the range of 9.60-12.37 mm and the fruit extract is in the range of 7.85-12.00 mm. MBC values ranged from 40 µg/µl to 80 µg/µl in peel extract and between 10 µg/µl and >80 µg/µl in the fruit extract. The inhibition zone diameters of the extracts against probiotics were determined as 6.10 -10.25 mm. As a result of the viable cell count, the viability rate of L. fermentum MA-7 increased as the fruit methanol extract concentration and time increased. The photoprotective activities of the peel and fruit extracts were determined as 13.17 and 12.26. It was determined that the extracts increased sun protection factor (SPF) value of the cream. The results indicated that H. polyrhizus extracts may have potential for use in the nutraceutical products, cosmetic and pharmaceutical industries. [ABSTRACT FROM AUTHOR]

Anthracnose is one of the destructive diseases of pitaya that seriously affects the plant growth and fruit quality and causes significant yield and economic losses worldwide. However, information regarding the species of pathogens that cause anthracnose in pitaya (Hylocereus undatus) fruits in Gansu Province, China, and its pathogenic mechanism is unknown. Thus, the purposes of our present study were to identify the species of pathogens causing H. undatus fruits anthracnose based on the morphological and molecular characteristics and determine its pathogenic mechanism by physiological and biochemical methods. In our present study, forty-six isolates were isolated from the collected samples of diseased H. undatus fruits and classified as three types (named as H-1, H-2, and H-3), according to the colony and conidium morphological characteristics. The isolation frequencies of H-1, H-2, and H-3 types were 63.04%, 21.74%, and 15.22%, respectively. The representative single-spore isolate of HLGTJ-1 in H-1 type has significant pathogenicity, and finally we identified Colletotrichum truncatum as the pathogen based on the morphological characteristics as well as multi-locus sequence analysis. Moreover, the H. undatus fruits inoculated with C. truncatum had a significantly increased activity of cell wall-degrading enzymes (CWDEs) cellulase (Cx), β-glucosidase (β-Glu), polygalacturonase (PG), and pectin methylgalacturonase (PMG), while having a decreased level of cell wall components of original pectin and cellulose in comparison to control. The average increased activities of Cx, β-Glu, PG, and PMG were 30.73%, 40.40%, 51.55%, and 32.23% from day 0 to 6 after inoculation, respectively. In contrast, the average decreased contents of original pectin and cellulose were 1.82% and 16.47%, respectively, whereas the average increased soluble pectin content was 38.31% in comparison to control. Our results indicate that C. truncatum infection increased the activities of CWDEs in H. undatus fruits to disassemble their cell wall components, finally leading to the fruits' decay and deterioration. Thus, our findings will provide significant evidence in the controlling of pitaya anthracnose in the future. [ABSTRACT FROM AUTHOR]

The present work intended to explore the synergistic effects of incorporating carbon nanotubes infused with magnesium oxide nanofluid and dragon fruit extract as a natural additive in photovoltaic–thermal collector systems on its performance in terms of both electrical and thermal energy yield. The proposed methodology was meticulously developed and implemented to assess the efficiency of a hybrid system integrating nano-phase change material and nanofluid in a photovoltaic–thermal configuration. This novel setup was subjected to a comprehensive comparative analysis against a traditional liquid-cooled photovoltaic–thermal system and a standalone photovoltaic module. The study also encompassed variations in cooling techniques, including water with different flow rates for the photovoltaic–thermal system, and the incorporation of nano-phase change material and nanofluid with varying concentrations and flow rates within the hybrid collector. The outcomes demonstrated that at these respective flow rates, the maximum thermal efficiency of the photovoltaic–thermal collector stood at 66.28% and 74.02%. Likewise, the peak electrical efficiency at the same flow rates was recorded as 20.79% and 21.96%. The outcomes of the investigation highlight the higher thermal conductivity of the nanofluid compared to the base fluid, leading to a slight augmentation in fluid density and viscosity. The results obtained from this investigation were found to be consistent with those documented in existing scientific literature. [ABSTRACT FROM AUTHOR]

The aim of this work was to determine the impact of pitahaya peel flour (PPF) addition, at different concentrations (1.5 and 3%) on the chemical, physico-chemical and sensorial properties of beef burgers. This assessment was based on: proximal analysis, physico-chemical and cooking properties, lipid oxidation and sensory assay of beef burgers. The burger reformulation with pitahaya peel flour had no effect (p > 0.05) on the chemical composition of raw burgers, whilst the cooked burger increased (p < 0.05) the protein and fat content and in the same way increased the calcium, potassium and phosphorus content (p < 0.05) with respect to the control sample. The colour coordinates redness (increased) and yellowness (decreased) were affected (p < 0.05) by the pitahaya peel flour addition. From a textural point of view, all parameters, except springiness, were affected (p < 0.05) by the incorporation of pitahaya peel flour. In addition, the use of pitahaya peel flour reduces (p < 0.05) the cooking loss and the shrinkage of burgers. The raw and cooked beef burger added with pitahaya peel flour showed lower lipid oxidation values (p < 0.05) than those of the control sample. The hedonic scores for all burgers analysed ranged from 6 to 7, which indicates a high degree of acceptance by the panellists. The results obtained indicated that pitahaya peel flour could be used as a novel ingredient in the development of beef burgers. [ABSTRACT FROM AUTHOR]

Owing to the increasing worldwide population explosion, managing waste generated from the food sector has become a cross-cutting issue globally, leading to environmental, economic, and social issues. Circular economy-inspired waste valorization approaches have been increasing steadily, generating new business opportunities developing valuable bioproducts using food waste, especially fruit wastes, that may have several applications in energy–food–pharma sectors. Dragon fruit waste is one such waste resource, which is rich in several value-added chemicals and oils, and can be a renewable resource to produce several value-added compounds of potential applications in different industries. Pretreatment and extraction processes in biorefineries are important strategies for recovering value-added biomolecules. There are different methods of valorization, including green extractions and biological conversion approaches. However, microbe-based conversion is one of the advanced technologies for valorizing dragon fruit waste into bioethanol, bioactive products, pharmaceuticals, and other valued products by reusing or recycling them. This state-of-the-art review briefly overviews the dragon fruit waste management strategies and advanced eco-friendly and cost-effective valorization technologies. Furthermore, various applications of different valuable bioactive components obtained from dragon fruit waste have been critically discussed concerning various industrial sectors. Several industrial sectors, such as food, pharmaceuticals, and biofuels, have been critically reviewed in detail. [ABSTRACT FROM AUTHOR]

This study aimed to investigate the effect of extraction and spray drying temperatures on the yield of bioactive materials during the production of dragon fruit skin extract powder as a natural food colorant. Extraction temperature was varied between 25 and 48 °C, and spray drying was conducted at 140, 170, and 190 °C. Moreover, the effects of spray drying aids were also evaluated. At extraction temperatures below 50 °C, higher temperatures resulted in a higher yield of bioactive materials and a significant increase in antioxidant activity. Regarding spray drying, the use of higher air inlet temperatures resulted in a peaking trend of yield from spray drying. However, employing high air inlet temperatures (above 170 °C) for spray drying may result in a decrease in the recoveries of bioactive materials due to the higher degradation rates. Finally, the addition of maltodextrin as a carrier agent in spray drying could enhance the powder yield. [ABSTRACT FROM AUTHOR]

The pitaya is a common fruit in southern China, but the growing environment of pitayas is complex, with a high density of foliage. This intricate natural environment is a significant contributing factor to misidentification and omission in the detection of the growing state of pitayas. In this paper, the growth states of pitayas are classified into three categories: flowering, immature, and mature. In order to reduce the misidentification and omission in the recognition process, we propose a detection model based on an improvement of the network structure of YOLOv8, namely YOLOv8n-CBN. The YOLOv8n-CBN model is based on the YOLOv8n network structure, with the incorporation of a CBAM attention mechanism module, a bidirectional feature pyramid network (BiFPN), and a C2PFN integration. Additionally, the C2F module has been replaced by a C2F_DCN module containing a deformable convolution (DCNv2). The experimental results demonstrate that YOLOv8n-CBN has enhanced the precision, recall, and mean average precision of the YOLOv8n model with an IoU threshold of 0.5. The model demonstrates a 91.1% accuracy, a 3.1% improvement over the original model, and an F1 score of 87.6%, a 3.4% enhancement over the original model. In comparison to YOLOv3-tiny, YOLOv5s, and YOLOv5m, which are highly effective target detection models, the mAP@0.50–0.95 of our proposed YOLOv8n-CBN is observed to be 10.1%, 5.0%, and 1.6% higher, respectively. This demonstrates that YOLOv8n-CBN is capable of more accurately identifying and detecting the growth status of pitaya in a natural environment. [ABSTRACT FROM AUTHOR]

Red dragon fruit (Hylocereus polyrhizus), recognized globally for its substantial nutrient content and health benefits, has been extensively studied; studies have particularly focused on the fruit, while the composition of the stem remains less explored. This research focuses on optimizing fermentation parameters for red dragon fruit wine, specifically examining yeast-strain selection, juice-to-water dilution ratios, and yeast concentrations. Saccharomyces cerevisiae RV002 emerged as the optimal strain due to its robust performance and adaptability under adverse conditions. The study identified a 50% dilution ratio as ideal for maximizing clarity and the sensory attributes of the wine, whereas dilution ratios exceeding 90% significantly reduced ethanol content below acceptable commercial standards. An optimal yeast concentration of 1 g/L was found to balance microbial suppression and alcohol yield effectively; deviations from this concentration led to microbial contamination or impaired fermentation dynamics. Fermentation markedly altered the biochemical properties of Hylocereus polyrhizus, reducing sugar and vitamin C levels while increasing polyphenol content and antioxidant activity, thereby enhancing potential health benefits. These findings underscore the transformative effects of microbial activity on the substrate's chemical landscape and highlight the potential of tailored fermentation strategies to enhance the utility and value of underutilized fruits in sustainable agricultural practices. [ABSTRACT FROM AUTHOR]

In this study, dragon fruit peels were subjected to different times (5, 10, 15, 20 and 25 min) of ultrasound pretreatment (4.870 W m−2/25°C) and then freeze‐drying (FD) (−56°C/48 h) to obtain powder particles, which underwent physical–chemical, functional and structural characterisation. Additionally, the extraction of total phenolic compound (TPC) was evaluated through ultrasound‐assisted kinetics and mechanical agitation. Finally, films based on black rice starch were developed with the addition (0.5, 1.5 and 2.5%) of dragon fruit peel powder particles, and their physical, barrier, antioxidant, structural and mechanical properties were evaluated. The results obtained showed that all particles had low water content (4.21%–6.58%) and high solubility (84.62%–88.73%) with a reduction in particle size (23.21–39.32 μm). In the extraction of TPC, the synergistic effect of the tested conditions increased efficiency, reaching 686.66 mg GAE/100 g for pretreated dragon fruit peel powder particles (25 min) in 180 min of extraction. Furthermore, the type 'A' crystallinity structure did not change significantly with pretreatment, but there was a reduction in peak area, reflecting on relative crystallinity, with a maximum decrease of 29.04%. The starch‐based films produced with 2.5% dragon fruit peel powder particles showed low solubility (28.72%) and low water vapour permeability (5.03 × 10−8 g mm s−1 m2 Pa), however, they contained higher levels of TPC (31.78 mg GAE/100 g film) and antioxidant activity (8.17 μmol TE/g film) that were easily migrated in aqueous medium. Furthermore, they also presented low values of tensile strength at break (3.41 MPa) and Young's modulus (4.18 MPa), as well as a high value of elongation at break (45.82%). Finally, our results highlight the potential of ultrasound as a pretreatment for FD of dragon fruit peels, paving the way for the development of rice starch‐based food packaging. [ABSTRACT FROM AUTHOR]

Dragon fruit stem disease significantly affects both the quality and yield of dragon fruit. Therefore, there is an urgent need for an efficient, high-precision intelligent detection method to address the challenge of disease detection. To address the limitations of traditional methods, including slow detection and weak micro-integration capability, this paper proposes an improved YOLOv8-G algorithm. The algorithm reduces computational redundancy by introducing the C2f-Faster module. The loss function was modified to the structured intersection over union (SIoU), and the coordinate attention (CA) and content-aware reorganization feature extraction (CARAFE) modules were incorporated. These enhancements increased the model's stability and improved its accuracy in recognizing small targets. Experimental results showed that the YOLOv8-G algorithm achieved a mean average precision (mAP) of 83.1% and mAP50:95 of 48.3%, representing improvements of 3.3% and 2.3%, respectively, compared to the original model. The model size and floating point operations per second (FLOPS) were reduced to 4.9 MB and 6.9 G, respectively, indicating reductions of 20% and 14.8%. The improved model achieves higher accuracy in disease detection while maintaining a lighter weight, serving as a valuable reference for researchers in the field of dragon fruit stem disease detection. [ABSTRACT FROM AUTHOR]

Dragon fruits have abundant nutritional and antioxidant properties. High temperatures limit the growth and production of dragon fruits. Hemin can effectively alleviate abiotic stress in plants. However, the regulatory effect of Hemin on dragon fruit under heat stress remains unclear. In this study, we explored the impacts of foliar application of Hemin on dragon fruit size, yield and quality during the high temperatures of the summer season. In this experiment, dragon fruit variety 'Jindu No. 1' was used as material and treated with three Hemin concentrations, i.e., H1: 1 μmol.L−1, H2: 10 μmol.L−1, H3: 100 μmol.L−1, compared with CK: control. The results show that exogenous Hemin increased the single fruit weight, yield, fruit shape index and edible rate. It also improved pericarp L* value, a* value, C* and decreased ho, improving the peel colour; exogenous Hemin enhanced soluble solids content and phenolic compounds content and antioxidant enzyme activities in the pulp of dragon fruit. In addition, exogenous Hemin increased the content of chlorophyll content in dragon fruit stems. Differential metabolites determined by metabolomic assay also indicated that Hemin significantly increased the content of active substances such as selagin. Additionally, the Hemin treatment H2 also activated the activity of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX), which helps to mitigate the effects of high temperatures on dragon fruit. The current findings strongly advocate that H2 treatment may effectively counteract the adverse effects of heat stress by regulating the morph-physiological and antioxidant traits. [ABSTRACT FROM AUTHOR]

Pitaya fruit is a significant agricultural commodity in southern China. The traditional method of determining the ripeness of pitaya by humans is inefficient, it is therefore of the utmost importance to utilize precision agriculture and smart farming technologies in order to accurately identify the ripeness of pitaya fruit. In order to achieve rapid recognition of pitaya targets in natural environments, we focus on pitaya maturity as the research object. During the growth process, pitaya undergoes changes in its shape and color, with each stage exhibiting significant characteristics. Therefore, we divided the pitaya into four stages according to different maturity levels, namely Bud, Immature, Semi-mature and Mature, and we have designed a lightweight detection and classification network for recognizing the maturity of pitaya fruit based on the YOLOv8n algorithm, namely GSE-YOLO (GhostConv SPPELAN-EMA-YOLO). The specific methods include replacing the convolutional layer of the backbone network in the YOLOv8n model, incorporating attention mechanisms, modifying the loss function, and implementing data augmentation. Our improved YOLOv8n model achieved a detection and recognition accuracy of 85.2%, a recall rate of 87.3%, an F1 score of 86.23, and an mAP50 of 90.9%, addressing the issue of false or missed detection of pitaya ripeness in intricate environments. The experimental results demonstrate that our enhanced YOLOv8n model has attained a commendable level of accuracy in discerning pitaya ripeness, which has a positive impact on the advancement of precision agriculture and smart farming technologies. [ABSTRACT FROM AUTHOR]

Pitaya, Hylocereus spp. (Caryophyllales: Cactaceae) has been one of the new species cultivated in Türkiye in recent years. There are many limiting factors, including pests, diseases and weeds, to decrease the yield and fruit quality in pitaya (dragon fruit) areas in Türkiye. In addition, the pests of pitaya is not fully studied therefore this research has been conducted to determine mealybug species in pitaya greenhouses in Türkiye. The present study aimed to determine mealybug species and its natural enemies (parasitoids, and predator insects) in pitaya greenhouses in Adana, Mersin, and Antalya between 2021 and 2022. According to results of this study, 2 different mealybug species was determined; Phenacoccus solenopsis Tinsley 1898, Phenacoccus madeirensis Green, 1923 (Hemiptera: Pseudococcidae) in pitaya greenhouses. Moreover one parasitoid (Aenasius arizonensis Girault (Hymenoptera: Encyrtidae), and two different predatory insects from 2 different orders and 2 different families (Chrysoperla carnea Stephens (Neuroptera: Chrysopidae), Nephus includens Kirsch (Coleoptera: Coccinellidae) were detected within this research. These two invasive mealybug species have wide spectrum host plants and may cause economically important damages to pitaya, if natural enemies cannot work successfully or control strategies do not applied properly in pitaya greenhouses. [ABSTRACT FROM AUTHOR]

Stem canker caused by Neocytalidium species poses a significant threat to the cultivation of dragon fruit (Hylocereus spp.). While traditional methods of identification and treatment require significant time and labor, the advancement of artificial intelligence (AI) offers a new frontier for precision agriculture. This paper proposes the development of an AI-based model to diagnose and treat stem canker in dragon fruit plants with greater accuracy, efficiency, and scalability. By leveraging machine learning algorithms, image recognition, and realtime data analysis, the proposed model could revolutionize agricultural disease management, enhancing crop yield and sustainability. [ABSTRACT FROM AUTHOR]

Under changing climatic conditions, plants are simultaneously facing conflicting stresses in nature. Plants can sense different stresses, induce systematic ROS signals, and regulate transcriptomic, hormonal, and stomatal responses. We performed transcriptome analysis to reveal the integrative stress response regulatory mechanism underlying heavy metal stress alone or in combination with heat and drought conditions in pitaya (dragon fruit). A total of 70 genes were identified from 31,130 transcripts with conserved differential expression. Furthermore, weighted gene co‐expression network analysis (WGCNA) identified trait‐associated modules. By integrating information from three modules and protein–protein interaction (PPI) networks, we identified 10 interconnected genes associated with the multifaceted defense mechanism employed by pitaya against co‐occurring stresses. To further confirm the reliability of the results, we performed a comparative analysis of 350 genes identified by three trait modules and 70 conserved genes exhibiting their dynamic expression under all treatments. Differential expression pattern of genes and comparative analysis, have proven instrumental in identifying ten putative structural genes. These ten genes were annotated as PLAT/LH2, CAT, MLP, HSP, PB1, PLA, NAC, HMA, and CER1 transcription factors involved in antioxidant activity, defense response, MAPK signaling, detoxification of metals and regulating the crosstalk between the complex pathways. Predictive analysis of putative candidate genes, potentially governing single, double, and multifactorial stress response, by several signaling systems and molecular patterns. These findings represent a valuable resource for pitaya breeding programs, offering the potential to develop resilient "super pitaya" plants. [ABSTRACT FROM AUTHOR]

Dextransucrases play a crucial role in the production of dextran from economical sucrose; therefore, there is a pressing demand to explore novel dextransucrases with better performance. This study characterized a dextransucrase enzyme, LmDexA, which was identified from the Leuconostoc mesenteroides NN710. This bacterium was isolated from the soil of growing dragon fruit in Guangxi province, China. We successfully constructed six different N-terminal truncated variants through sequential analysis. Additionally, a truncated variant, ΔN190LmDexA, was constructed by removing the 190 amino acids fragment from the N-terminal. This truncated variant was then successfully expressed heterologously in Escherichia coli and purified. The purified ΔN190LmDexA demonstrated optimal hydrolysis activity at a pH of 5.6 and a temperature of 30 °C. Its maximum specific activity was measured to be 126.13 U/mg, with a Km of 13.7 mM. Results demonstrated a significant improvement in the heterologous expression level and total enzyme activity of ΔN190LmDexA. ΔN190LmDexA exhibited both hydrolytic and transsaccharolytic enzymatic activities. When sucrose was used as the substrate, it primarily produced high-molecular-weight dextran (>400 kDa). However, upon the addition of maltose as a receptor, it resulted in the production of a significant amount of oligosaccharides. Our results can provide valuable information for enhancing the characteristics of recombinant dextransucrase and potentially converting sucrose into high-value-added dextran and oligosaccharides. [ABSTRACT FROM AUTHOR]

Cavendish banana and red dragon fruit contain vitamins, fiber, and antioxidants. However, the fruits are quickly spoiled, and requires processing into instant powder with the foam mat drying for extend the storage time. Okara protein hydrolysate through enzymatic hydrolysis process and soy protein isolate will be used as foaming agent. This study evaluated the effect of adding okara protein hydrolysate and soy protein isolate on the physicochemical properties such as water absorption index (WAI), water solubility index (WSI), hygroscopicity, bulk density, color, viscosity, antioxidant activity, vitamin C, total soluble solid, titratable acidity, proximate composition, acceptance test, and shelf life of instant powder. The results showed that the instant powder with okara protein hydrolysate produced a moisture content of 2.81%, WAI 0.69 g/g, and bulk density 0.39 g/cm3, lower than the addition of soy protein isolate. The acceptance test of instant powder with okara protein hydrolysate had an average score of 4.40 (neutral) to 5.06 (slightly like). The addition of okara hydrolysate can significantly (p < 0.05) increased the WSI value to 81.42%, antioxidant activity 49.86%, and longer shelf life of 144 days. Therefore, okara protein hydrolysate can be used as foaming agents with natural antioxidants and produced the stable instant powder. [ABSTRACT FROM AUTHOR]

Dragon fruit has significant economic value in many countries due to has excellent nutritional content, health advantages, and adaptability to different climates, making it an important crop in the global fruit industry. This study aimed to gather comprehensive nutritional data on three dragon fruit cultivars by analysing the levels of micronutrients, fibre, carbohydrates, antioxidants, vitamins, and minerals in their pulps. Uniform dragon fruit samples underwent thorough analysis for proximate composition, mineral content, pigments, antioxidants, and vitamin C, with statistical methods used to assess significant differences among the parameters studied. The proximate composition analysis revealed significant differences among the three dragon fruit cultivars. Among the proximate components, protein (0.40 ± 0.02 g/100 g), moisture (91.33 ± 0.88%), crude fibre (0.32 ± 0.07 g/100 g), and ash (1.27 ± 0.09 g/100 g) were more abundant in Hylocereus costaricensis than in Hylocereus undatus and Hylocereus megalanthus. On the other hand, Hylocereus undatus had higher carbohydrate (17.02 ± 0.63 g/100 g) and energy (69.74 ± 2.44 kcal/100 g) contents. K (7.23 ± 0.35 mg/100 g), Ca (1.61 ± 0.13 mg/100 g), Fe (1.84 ± 0.05 mg/100 g), and Zn (0.37 ± 0.034 mg/100 g) are highly abundant in H. costaricensis. Additionally, Hylocereus costaricensis had the highest anthocyanin content (120.15 ± 3.29 mg/g FW) and total carotenoid content (72.51 ± 1.62 mg/g FW), along with the highest vitamin C content (8.92 ± 0.13 mg/g FW) and total soluble phenolic content (572.48 ± 20.77 mg/100 g). Its remarkable antioxidant activity was further highlighted by the lowest SC50 value (13.50 ± 0.4 mg/mL) for its DPPH radical scavenging capacity. The total soluble sugar content was highest in Hylocereus megalanthus (8.72 ± 0.30 g/100 g FW). Hierarchical clustering analysis revealed distinct trait and genotype associations; among the studied cultivars, Hylocereus costaricensis demonstrated superior performance across multiple traits. Correlation analysis indicated significant positive correlations among several traits, while principal component analysis highlighted the contribution of each trait to overall variance, with PC1 explaining 73.95% of the total variance. This study highlights the nutritional variations among dragon fruit cultivars, with Hylocereus costaricensis showing superior performance, guiding dietary planning and functional food development. [ABSTRACT FROM AUTHOR]

The objective of this study was to investigate the effect of microencapsulated bioactive compounds from lemongrass mixed dragon fruit peel pellet (MiEn-LEDRAGON) supplementation on fermentation characteristics, nutrient degradability, methane production, and the microbial diversity using in vitro gas production technique. The study was carried out using a completely randomized design (CRD) with five levels of MiEn-LEDRAGON supplementation at 0, 1, 2, 3, and 4% of the total dry matter (DM) substrate. Supplementation of MiEn-LEDRAGON in the diet at levels of 3 or 4% DM resulted in increased (p < 0.05) cumulative gas production at 96 hours (h) of incubation time, reaching up to 84.842 ml/ 0.5 g DM. Furthermore, supplementation with 3% MiEn-LEDRAGON resulted in higher in vitro nutrient degradability and ammonia–nitrogen concentration at 24 h of the incubation time when compared to the control group (without supplementation) by 5.401% and 11.268%, respectively (p < 0.05). Additionally, supplementation with MiEn-LEDRAGON in the diet led to an increase in the population of Fibrobacter succinogenes at 24 h and Butyrivibrio fibrisolvens at 12 h, while decreasing the population of Ruminococcus albus, Ruminococcus flavefaciens, and Methanobacteriales (p < 0.05). Moreover, supplementation of MiEn-LEDRAGON in the diet at levels of 2 to 4% DM resulted in a higher total volatile fatty acids (VFA) at 24 h, reaching up to 73.021 mmol/L (p < 0.05). Additionally, there was an increased proportion of propionic acid (C3) and butyric acid (C4) at 12 h (p < 0.05). Simultaneously, there was a decrease in the proportion of acetic acid (C2) and the ratio of acetic acid to propionic acid (C2:C3), along with a reduction of methane (CH4) production by 11.694% when comparing to the 0% and 3% MiEn-LEDRAGON supplementation (p < 0.05). In conclusion, this study suggests that supplementing MiEn-LEDRAGON at 3% of total DM substrate could be used as a feed additive rich in phytonutrients for ruminants. [ABSTRACT FROM AUTHOR]

The usage of natural food colorants in food processing is increasing nowadays. Red dragon fruit peel is rich in betacyanin pigment. The peel, which is generally categorized as food waste, can be extracted and used as a natural food colorant that produces a red-violet color. Pigment extraction using ohmic heating can shorten the extraction time. Ohmic heating has a very fast heating rate and can avoid excessive thermal damage to pigments. This study aims to determine the effect of solvent type and extraction temperature on chemical and physical characteristics, as well as process parameters (heating time, electrical conductivity, and ohmic heating efficiency) of extracts that have been extracted using the ohmic heating method. The study used a 2-factor group randomized design, namely solvent type (distilled water, citric acid 0.2%, and NaCl 0.2%) and extraction temperature (50, 60, 70, 80, and 90°). The results showed that the interaction between solvent type and extraction temperature significantly affected betacyanin content. Each factor (solvent type and extraction temperature) had a significant effect on the heating time, electrical conductivity, and extraction yield. NaCl 0.2% solvent can increase electrical conductivity so that the heating time is faster and pigments can be extracted from the cells. Ohmic heating can be used for the extraction process of betacyanin pigment from red dragon fruit peel, which is heat-labile. [ABSTRACT FROM AUTHOR]

In order to effectively solve the problems of the complex growth state of dragon fruit and how the picking process is mostly manual, this study designed a picking and selecting integrated remote-operation-type dragon-fruit-picking device. Based on SOLIDWORKS 2020 software for the three-dimensional digital design and overall assembly of key components, the structure and working theory of the machine are introduced. By improving the high-recognition-rate dragon fruit target detection algorithm based on YOLOv5, better recognition and locating effects were achieved for targets with a small size and high density, as well as those in bright-light scenes. Serial communication, information acquisition, and the precise control of each picking action were realized by building the software and hardware platforms of the picking device control system. By analyzing the working principle of the mechanical system and the mechanics of the machine picking process, the critical factors affecting the net picking rate and damage rate of the dragon-fruit-picking device were confirmed. Based on the force and parameter analysis of the test results, it was confirmed that the machine had an optimal picking influence when the flexible claw closing speed was 0.029 m/s, the electric cylinder extending speed was 0.085 m/s, and the mechanical arm moving speed was 0.15 m/s. The net picking rate of the device reached 90.5%, and the damage rate reached 2.9%. The picking device can complete the picking of a single dragon fruit, as well as a plurality of fruits grown at a growing point, and integrates the integration of picking fruits, removing bad fruits, and sorting fruits, which can improve the efficiency of dragon fruit harvesting and replace manual work. [ABSTRACT FROM AUTHOR]

Red dragon fruit is gaining popularity globally due to its nutritional value and bioactive components. The study aimed to assess the phytochemical, nutritional composition, antioxidant, antibacterial, and cytotoxic properties of extracts from the South Chinese red dragon fruit peel, flesh, and seeds. Extract fractions with increasing polarity (ethyl acetate50 values ranging from 3.25–8.30 μg/mL. The flesh MT and ET extract fractions demonstrated substantial inhibitory effects, with MIC50 ranging 73.62–98.85 μg/mL, against several multidrug-resistant bacteria. Cytotoxicity assays revealed no adverse effects of the crude extract fractions on human keratinocytes (HaCaT) cells (from adult human skin). Liquid chromatography with tandem mass spectrometry (LC-MS/MS) and gas chromatography-tandem mass spectrometry (GC-MS/MS) analyses unveiled a wide array of compounds with known and unknown antibacterial and antioxidant activities. Thereafter, pharmacological property screening and molecular docking were performed. The results identified four active compounds, baicalein, quercetin, morin, and isorhamnetin, which demonstrated strong binding affinity with the selected target proteins and complied with most of the pharmacological parameters. In conclusion, this study provides essential information on the key nutrients present in the peel, flesh, and seeds of the red dragon fruit from Southern-China. Furthermore, it highlights the potential of ET and MT fractions, which contain compounds with promising antibacterial properties. [ABSTRACT FROM AUTHOR]

Dragon fruit (Hylocereus spp.) is an exotic vine cactus, rich in vitamin C and antioxidants, commonly known as 'pitaya' is a weather resilient crop. In order to develop superior varieties, a large number of progenies obtained from open pollinated seedling of cvs. Hiryur red, Vietnam pink and Vietnam red were raised and evaluated. To broaden the genetic base with desired traits, seeds of Hirehalli red and Hirehalli white were irradiated with gamma rays (500, 600 and 700 Gy), EMS (2.25, 2.5 and 2.75%) and sodium azide (0.04, 0.05 and 0.06%) and evaluated for horticultural traits. Three elite progenies such as CHESH-D1, CHESH-D2, and CHESH-D3 were obtained from open pollinated seedlings of cvs. Hiryur red, Vietnam red and Vietnam pink, respectively, and one EMS (2.5%) treated mutant i.e. CHESH-DE were selected based on their morphological and yield traits. The selection CHESH-D1 performed superior with highest fruit weight (507.71 g) and yield (39.50 kg/pole) compared to CHESH-D2 and CHESH-D3. The biochemical characters namely, TSS (15.35 oB), total sugars (5.95 g), reducing sugars (4.91 g) and betalains (21.2 mg BCE) were recorded highest in CHESH-D2, and one EMS (2.5%) treated mutant from cv. Hirehalli red showed better adaptability and dwarfness. These elite progenies are at final stage of performance assessment to be released as variety in India. [ABSTRACT FROM AUTHOR]

The oriental fruit fly Bactrocera dorsalis (Hendel) has been reported on the white fleshed dragon fruit Selenicereus undatus (Haworth) D.R.Hunt for the first time in India. Symptoms of infestation and the duration of all life stages were observed. Females laid eggs just beneath the mature fruit skin. The tissue surrounding the egg mass turned light yellow. Oviposition punctures were visible on the fruit rind and the feeding of the maggots led to decay of internal contents, foul smell and the fruit turned in to a discolored semi liquid mass. Full grown maggots exited the fruit and entered a period of inactivity before pupation in moist soil. The egg, maggot and pupal stages lasted for 1.6± 0.40, 8.0 ± 0.40 and 8.0 ± 0.31 days, respectively under laboratory conditions. Adult longevity was 9.6 ± 0.5 days in the case of females and 6.4 ± 0.50 days for males. [ABSTRACT FROM AUTHOR]

The synthesis of betalain using microorganisms is an innovative developmental technology, and the excavation of microorganisms closely related to betalain can provide certain theoretical and technical support to this technology. In this study, the characteristics of soil microbial community structures and their functions in the rhizospheres of white-fleshed dragon fruit (Hylocereus undatus) and red-fleshed dragon fruit (Hylocereus polyrhizus) were analyzed. The results show that the soil bacterial and fungal compositions in the rhizospheres were shaped differently between H. undatus and H. polyrhizus. Bacterial genera such as Kribbella and TM7a were the unique dominant soil bacterial genera in the rhizospheres of H. undatus, whereas Bradyrhizobium was the unique dominant soil bacterial genus in the rhizospheres of H. polyrhizus. Additionally, Myrothecium was the unique dominant soil fungal genus in the rhizospheres of H. polyrhizus, whereas Apiotrichum and Arachniotus were the unique dominant soil fungal genera in the rhizospheres of H. undatus. Moreover, TM7a, Novibacillus, Cupriavidus, Mesorhizobium, Trechispora, Madurella, Cercophora, and Polyschema were significantly enriched in the rhizospheres of H. undatus, whereas Penicillium, Blastobotrys, Phialemonium, Marasmius, and Pseudogymnoascus were significantly enriched in the rhizospheres of H. polyrhizus. Furthermore, the relative abundances of Ascomycota and Penicillium were significantly higher in the rhizospheres of H. polyrhizus than in those of H. undatus. [ABSTRACT FROM AUTHOR]