1. Role of zein incorporation on hydrophobic drug-loading capacity and colloidal stability of phospholipid nanoparticles.
- Author
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Hong, Soon-Seok, Thapa, Raj Kumar, Kim, Jin-Hee, Kim, Soo-Yeon, Kim, Jong Oh, Kim, Jin-Ki, Choi, Han-Gon, and Lim, Soo-Jeong
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PHOSPHOLIPIDS , *NANOPARTICLES , *LECITHIN , *DRUG carriers , *DRUG delivery systems , *LIPOPROTEIN drug carriers , *NANOCAPSULES - Abstract
Graphical abstract Highlights • Zein-phosphatidylcholine hybrid nanoparticles were produced. • Zein incorporation increased the hydrophobic drug-loading capacity. • Zein functioned as a stabilizer of PC-NP in serum- and storage condition. • Zein functioned as a cryoprotectant of PC-NP. Abstract Liposome, phosphatidylcholine nanoparticle (PC-NP), is an attractive colloidal carrier of hydrophobic drugs but its clinical development is often limited by low drug-loading capacity and the physical instability. Zein is a water-insoluble amphiphilic protein obtained from the corn. We herein investigated a possibility to develop zein-phosphatidylcholine hybrid nanoparticle (Z/PC-NP) as an advanced hydrophobic drug carrier. By employing the conventional liposome preparation method with the addition of zein, Z/PC-NP were produced. The extent of zein incorporation in PC-NP was affected by PC composition. DSC demonstrated the lowered phase transition temperature of PC by zein and FTIR showed the appearance of weakened but clear amide bonds of zein as well as increased levels of heterogeneous hydrogen bonding of Z/PC-NP compared to PC-NP. DLS, TEM and cryo-TEM studies suggested Z/PC-NP to be spherical nanoparticles composed of a zein core and a zein-PC hybrid shell. Z/PC-NP exhibited a higher loading capacity for hydrophobic model drugs (paclitaxel, docetaxel, celecoxib and curcumin), than did the zein nanoparticle and PC-NP, while exhibiting an intermediate drug release rate. The serum stability and the storage stability of Z/PC-NP were greater than those of PC-NP. Zein functioned as a cryoprotectant of PC-NP during freeze-drying. Z/PC-NP may provide a promising nanoparticle carrier of hydrophobic drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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