4,645 results on '"*DESULFOVIBRIO"'
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2. Application of Bacteriophages to Treat Toxic Gas-producing Bacteria Desulfovibrio spp. in Shrimp Ponds.
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Truong Thi Bich Van, Tran Vo Minh Thu, Van-Thanh Vo, and Nguyen Thi Loan Anh
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SHRIMP culture , *BACTERIOPHAGES , *DESULFOVIBRIO , *SHRIMP diseases , *HYDROGEN sulfide - Abstract
Desulfovibrio spp. bacteria pose significant challenges in shrimp aquaculture by producing hydrogen sulfide (H2S), which depletes oxygen levels and hinders shrimp growth. The increasing prevalence of antibiotic-resistant bacteria necessitates the exploration of alternative control methods. This study investigates the potential of bacteriophages as a biological solution to inhibit Desulfovibrio spp. in shrimp ponds. Six bacteriophage strains (ST231, ST22, fMix 1, fS8, fS9, and fMix 2) were evaluated using plaque assays at three time points (3, 6, and 18 hours). Results demonstrated that most bacteriophages significantly reduced bacterial density over time, with ST22 showing the most substantial inhibition (p<0.05). Genomic analysis of bacteriophage ST22, published in the GenBank database, revealed genes related to Desulfovibrio spp., specifically Deltaproteobacteria and Desulfobacteraceae. The study also found that bacteriophage treatment led to decreased H2S and NH3 levels and increased dissolved oxygen in simulated pond environments. Morphological changes in bacterial colonies post-treatment further supported the phages' inhibitory effects. These findings suggest that bacteriophage ST22 is a promising biological alternative to antibiotics for preventing and controlling diseases caused by Desulfovibrio vulgaris in shrimp aquaculture, though further research is needed to assess long-term ecological impacts and efficacy in complex pond ecosystems. [ABSTRACT FROM AUTHOR] more...
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- 2024
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3. Sulfate-Reducing Bacteria Isolated from an Oil Field in Kazakhstan and a Description of Pseudodesulfovibrio karagichevae sp. nov.
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Bidzhieva, Salimat K., Tourova, Tatyana P., Grouzdev, Denis S., Samigullina, Salima R., Sokolova, Diyana S., Poltaraus, Andrey B., Avtukh, Alexander N., Tereshina, Vera M., Mardanov, Andrey V., Zhaparov, Nurlan S., and Nazina, Tamara N. more...
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OIL field brines ,OIL fields ,FUNCTIONAL genomics ,PETROLEUM reservoirs ,SULFATE-reducing bacteria - Abstract
Sulfidogenic bacteria cause numerous issues in the oil industry since they produce sulfide, corroding steel equipment, reducing oil quality, and worsening the environmental conditions in oil fields. The purpose of this work was to isolate and taxonomically identify the sulfidogenic bacteria responsible for the corrosion of steel equipment at the Karazhanbas oil field (Kazakhstan). In this study, we characterized five sulfidogenic strains of the genera Pseudodesulfovibrio, Oleidesulfovibrio, and Acetobacterium isolated from the formation water of the Karazhanbas oil field (Kazakhstan). Sulfate-reducing strain 9FUS
T revealed 98.9% similarity of the 16S rRNA gene sequence with the closely related strain 'Pseudodesulfovibrio methanolicus' 5S69T and was studied in detail to enhance the taxonomic resolution. Strain 9FUST grew optimally at 23–28 °C, pH 6.5, and 0–2% (w/v) NaCl. The strain used lactate, pyruvate, methanol, ethanol, fructose, ribose, and H2 /CO2 (in the presence of acetate) as carbon and energy sources for sulfate reduction. Iso-C17:1 ω11, C15:0 , iso-C15:0 , and C16:0 were the predominant fatty acids. The genome is 4.20 Mbp with a G + C content of 64.0%. The average nucleotide identity and digital DNA–DNA hybridization values with Pseudodesulfovibrio spp. genomes were 72.5–91.6% (<95%) and 18.5–45.0% (<70%), respectively, and supported our conclusion that 9FUST (=VKM B-3654T = KCTC 25498T ) belonged to a novel Pseudodesulfovibrio species, for which the name Pseudodesulfovibrio karagichevae sp. nov. is proposed. Pangenome analysis of sixteen Pseudodesulfovibrio species and functional annotation analysis of identified genes revealed complete modules of enzymes of the main metabolic pathways, characteristic of bacteria of this genus, and unique genes highlighting the adaptations of strain 9FUST in carbohydrate metabolism, nutrient uptake, and environmental stress response. Isolation of these strains expands our understanding of the diversity of sulfidogens in oil reservoirs and can be used to test the effectiveness of biocides used in an oil field. [ABSTRACT FROM AUTHOR] more...- Published
- 2024
- Full Text
- View/download PDF
4. Abcesso hepático e Bacteriemia Causada por Desulfovibrio Desulfuricans
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Tiago Guimarães Costa, Raquel Moura, Luís Rocha, Sara Pinto, and Paula Ferreira
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absceso hepático ,bacteriemia ,desulfovibrio ,portugal ,metronidazol ,Medicine ,Internal medicine ,RC31-1245 - Abstract
This case report presents a rare liver abscess caused by Desulfovibrio desulfuricans bacteremia in an elderly Indian patient. Desulfovibrio spp. are gram-negative, sulfate-reducing anaerobic bacteria that are typically found in the environment and can also be normal inhabitants of the oral cavity and intestinal tract. While liver abscesses are commonly caused by gram-negative bacilli, Desulfovibrio spp. are rarely reported as causative agents. The patient, an 83-year-old Indian female, was living in Portugal for a long time. She had multiple comorbidities and presented with general malaise, asthenia associated with decreased appetite, and diarrhea. CT scan confirmed the presence of liver abscesses and blood cultures identified Desulfovibrio desulfuricans as the causative agent. Treatment involved percutaneous drainage of the abscesses and a 6-week course of antibiotics with metronidazole initially and then with amoxicillin/clavulanic acid. The patient showed clinical improvement, and subsequent negative haemocultures and imaging control indicated the effectiveness of the treatment. However, the patient's condition deteriorated due to respiratory failure caused by COVID-19, highlighting the impact of underlying comorbidities. The rarity of Desulfovibrio desulfuricans infections in Portugal further emphasizes the significance of this case. It raises questions about the potential influence of ethnicity and geography on susceptibility to such infections, warranting further research. more...
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- 2025
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5. Corrosion behavior of predominant Halodesulfovibrio in a marine SRB consortium and its mitigation using ZnO nanoparticles
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Mansour Jafari, Hamid Moghimi, Hassan Tirandaz, and Mohammad-Bagher Ebrahim-Habibi
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Consortium ,Halodesulfovibrio ,Microbial corrosion ,ZnO ,Medicine ,Science - Abstract
Abstract Formation of Sulfate Reducing Bacteria (SRB) biofilm accelerates microbiologically influenced corrosion (MIC). The aim of this study was to investigate both the corrosivity of a marine SRB consortium on carbon steel coupons and its mitigation in the presence of ZnO. Metagenomics analysis revealed that Halodesulfovibrio (78.9%) was predominant and could be related to MIC. The analysis also showed a remarkable shift from a highly corrosive SRB consortium in the control bioreactors to a far less corrosive consortium when ZnO was added to the bioreactors. Further results indicated that the corrosion rate of the SRB consortium was 8.17 mpy on the carbon steel coupons. In the ZnO-treated bioreactors, the count of SRB and MIC in the carbon steel coupons simultaneously reduced. Moreover, Confocal Laser Scanning Microscopy and profilometry analysis determined that ZnO could significantly decrease the amount of biofilm and the corrosion rate. Electrochemical experiments revealed higher corrosion current density (i corr ) and lower charge transfer resistance (Rct) in the control bioreactors relative to the ZnO-treated bioreactors. We introduce Halodesulfovibrio as a potentially important corrosive genus in a marine SRB consortium. Additionally, ZnO could be considered a proper candidate to control the corrosion induced by Halodesulfovibrio. more...
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- 2024
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6. Phenotypic and Genomic Characterization of a Sulfate-Reducing Bacterium Pseudodesulfovibrio methanolicus sp. nov. Isolated from a Petroleum Reservoir in Russia.
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Bidzhieva, Salimat K., Tourova, Tatyana P., Kadnikov, Vitaly V., Samigullina, Salima R., Sokolova, Diyana S., Poltaraus, Andrey B., Avtukh, Alexander N., Tereshina, Vera M., Beletsky, Alexey V., Mardanov, Andrey V., and Nazina, Tamara N. more...
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SULFATE-reducing bacteria , *PETROLEUM reservoirs , *OIL fields , *GENOMICS , *WATER temperature - Abstract
Simple Summary: Corrosion of steel equipment during oil production, transportation, and refining is a big global issue, leading to significant economic losses. The main agents of microbially influenced corrosion (MIC) of steel equipment are sulfate-reducing bacteria (SRB) and archaea (SRA), which reduce sulfate present in the reservoir water to form sulfide. Timely detection of sulfidogens in formations is necessary for the development of measures to suppress their growth. Existing 16S rRNA gene-based molecular methods for the detection of sulfidogens make it possible to identify them in their natural habitat, and the methods based on metagenomic analysis of components of the microbial community make it possible to predict their potential functional activity. However, selection of biocides or other methods for suppressing the growth of sulfidogens requires confirmation of their effectiveness on enrichment and/or pure cultures. In order to establish a collection of sulfidogens inhabiting the oil reservoirs of Tatarstan (Russia), a number of strains of sulfate-reducing bacteria were isolated. This study describes the 5S69T strain, which, based on the physiological and biochemical characteristics and genomic analysis, has been assigned to a new species, Pseudodesulfovibrio methanolicus sp. nov. The strain is able to grow at high salinity, at reservoir temperature, and on media with alcohols or H2/CO2 in the presence of acetate, which indicates its adaptation to environmental conditions and potential in sulfide production in the oil reservoir. The search for the microorganisms responsible for sulfide formation and corrosion of steel equipment in the oil fields of Tatarstan (Russia) resulted in the isolation of a new halotolerant strictly anaerobic sulfate-reducing bacterium, strain 5S69T. The cells were motile curved Gram-negative rods. Optimal growth was observed in the presence of 2.0–4.0% (w/v) NaCl, at pH 6.5, and at 23–28 °C under sulfate-reducing conditions. The isolate was capable of chemoorganotrophic growth with sulfate and other sulfoxides as electron acceptors, resulting in sulfide formation; and of pyruvate fermentation resulting in formation of H2 and acetate. The strain utilized lactate, pyruvate, ethanol, methanol, fumarate, and fructose, as well as H2/CO2/acetate for sulfate reduction. The genome size of the type strain 5S69T was 4.16 Mb with a G + C content of 63.0 mol%. On the basis of unique physiological properties and results of the 16S rRNA gene-based phylogenetic analysis, phylogenomic analysis of the 120 conserved single copy proteins and genomic indexes (ANI, AAI, and dDDH), assigning the type strain 5S69T ((VKM B-3653T = KCTC 25499T) to a new species within the genus Pseudodesulfovibrio, is suggested, with the proposed name Pseudodesulfovibrio methanolicus sp. nov. Genome analysis of the new isolate showed several genes involved in sulfate reduction and its sulfide-producing potential in oil fields with high saline formation water. [ABSTRACT FROM AUTHOR] more...
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- 2024
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7. Corrosion behavior of predominant Halodesulfovibrio in a marine SRB consortium and its mitigation using ZnO nanoparticles.
- Author
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Jafari, Mansour, Moghimi, Hamid, Tirandaz, Hassan, and Ebrahim-Habibi, Mohammad-Bagher
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MICROBIOLOGICALLY influenced corrosion ,SULFATE-reducing bacteria ,CARBON steel ,ZINC oxide ,NANOPARTICLES ,CHARGE transfer - Abstract
Formation of Sulfate Reducing Bacteria (SRB) biofilm accelerates microbiologically influenced corrosion (MIC). The aim of this study was to investigate both the corrosivity of a marine SRB consortium on carbon steel coupons and its mitigation in the presence of ZnO. Metagenomics analysis revealed that Halodesulfovibrio (78.9%) was predominant and could be related to MIC. The analysis also showed a remarkable shift from a highly corrosive SRB consortium in the control bioreactors to a far less corrosive consortium when ZnO was added to the bioreactors. Further results indicated that the corrosion rate of the SRB consortium was 8.17 mpy on the carbon steel coupons. In the ZnO-treated bioreactors, the count of SRB and MIC in the carbon steel coupons simultaneously reduced. Moreover, Confocal Laser Scanning Microscopy and profilometry analysis determined that ZnO could significantly decrease the amount of biofilm and the corrosion rate. Electrochemical experiments revealed higher corrosion current density (i
corr ) and lower charge transfer resistance (Rct ) in the control bioreactors relative to the ZnO-treated bioreactors. We introduce Halodesulfovibrio as a potentially important corrosive genus in a marine SRB consortium. Additionally, ZnO could be considered a proper candidate to control the corrosion induced by Halodesulfovibrio. [ABSTRACT FROM AUTHOR] more...- Published
- 2024
- Full Text
- View/download PDF
8. Low dose rifaximin combined with N-acetylcysteine is superior to rifaximin alone in a rat model of IBS-D: a randomized trial.
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Leite, Gabriela, Rezaie, Ali, Morales, Walter, Weitsman, Stacy, de Freitas Germano, Juliana, Barlow, Gillian M., Parodi, Gonzalo, Pimentel, Maya L., Villanueva-Millan, Maria Jesus, Sanchez, Maritza, Ayyad, Sarah, Mathur, Ruchi, and Pimentel, Mark more...
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LABORATORY rats , *ESCHERICHIA coli , *IRRITABLE colon , *RIFAXIMIN , *SMALL intestine - Abstract
Rifaximin is FDA-approved for treatment of irritable bowel syndrome with diarrhea (IBS-D), but poor solubility may limit its efficacy against microbes in the mucus layer, e.g. Escherichia coli. Here we evaluate adding the mucolytic N-acetylcysteine (NAC) to improve rifaximin efficacy. In a resazurin checkerboard assay, combining rifaximin with NAC had significant synergistic effects in reducing E. coli levels. The optimal rifaximin + NAC combination was then tested in a validated rat model of IBS-D (induced by cytolethal distending toxin [CdtB] inoculation). Rats were inoculated with vehicle and treated with placebo (Control-PBS) or rifaximin + NAC (Control-Rif + NAC, safety), or inoculated with CdtB and treated with placebo (CdtB-PBS), rifaximin (CdtB-Rifaximin), or rifaximin + NAC (CdtB-Rif + NAC) for 10 days. CdtB-inoculated rats (CdtB-PBS) developed wide variability in stool consistency (P = 0.0014) vs. controls (Control-PBS). Stool variability normalized in rats treated with rifaximin + NAC (CdtB-Rif + NAC) but not rifaximin alone (CdtB-Rifaximin). Small bowel bacterial levels were elevated in CdtB-PBS rats but normalized in CdtB-Rif + NAC but not CdtB-Rifaximin rats. E. coli and Desulfovibrio spp levels (each associated with different IBS-D microtypes) were also elevated in CdtB-inoculated (CdtB-PBS) but normalized in CdtB-Rif + NAC rats. Cytokine levels normalized only in CdtB-Rif + NAC rats, in a manner predicted to be associated with reduced diarrhea driven by reduced E. coli. These findings suggest that combining rifaximin with NAC may improve the percentage of IBS-D patients responding to treatment. [ABSTRACT FROM AUTHOR] more...
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- 2024
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9. The Role of Akkermansia muciniphila on Improving Gut and Metabolic Health Modulation: A Meta-Analysis of Preclinical Mouse Model Studies.
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Khalili, Leila, Park, Gwoncheol, Nagpal, Ravinder, and Salazar, Gloria
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WEIGHT loss ,EXTRACELLULAR vesicles ,GUT microbiome ,ANIMAL models in research ,BODY weight - Abstract
Akkermansia muciniphila (A. muciniphila) and its derivatives, including extracellular vesicles (EVs) and outer membrane proteins, are recognized for enhancing intestinal balance and metabolic health. However, the mechanisms of Akkermansia muciniphila's action and its effects on the microbiome are not well understood. In this study, we examined the influence of A. muciniphila and its derivatives on gastrointestinal (GI) and metabolic disorders through a meta-analysis of studies conducted on mouse models. A total of 39 eligible studies were identified through targeted searches on PubMed, Web of Science, Science Direct, and Embase until May 2024. A. muciniphila (alive or heat-killed) and its derivatives positively affected systemic and gut inflammation, liver enzyme level, glycemic response, and lipid profiles. The intervention increased the expression of tight-junction proteins in the gut, improving gut permeability in mouse models of GI and metabolic disorders. Regarding body weight, A. muciniphila and its derivatives prevented weight loss in animals with GI disorders while reducing body weight in mice with metabolic disorders. Sub-group analysis indicated that live bacteria had a more substantial effect on most analyzed biomarkers. Gut microbiome analysis using live A. muciniphila identified a co-occurrence cluster, including Desulfovibrio, Family XIII AD3011 group, and Candidatus Saccharimonas. Thus, enhancing the intestinal abundance of A. muciniphila and its gut microbial clusters may provide more robust health benefits for cardiometabolic, and age-related diseases compared with A. muciniphila alone. The mechanistic insight elucidated here will pave the way for further exploration and potential translational applications in human health. [ABSTRACT FROM AUTHOR] more...
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- 2024
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10. Syntrophic Interactions Ameliorate Arsenic Inhibition of Solvent-Dechlorinating Dehalococcoides mccartyi
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Gushgari-Doyle, Sara, Olivares, Christopher I, Sun, Mohan, and Alvarez-Cohen, Lisa
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Biological Sciences ,Biomedical and Clinical Sciences ,Pollution and Contamination ,Environmental Sciences ,Medical Biochemistry and Metabolomics ,Nutrition ,Foodborne Illness ,Solvents ,Arsenic ,Arsenites ,Trichloroethylene ,Lactic Acid ,microbial interactions ,TCE ,arsenic ,dechlorination ,co-culture ,Dehalococcoides ,Desulfovibrio - Abstract
Interactions and nutrient exchanges among members of microbial communities are important for understanding functional relationships in environmental microbiology. We can begin to elucidate the nature of these complex systems by taking a bottom-up approach utilizing simplified, but representative, community members. Here, we assess the effects of a toxic stress event, the addition of arsenite (As(III)), on a syntrophic co-culture containing lactate-fermenting Desulfovibrio vulgaris Hildenborough and solvent-dechlorinating Dehalococcoides mccartyi strain 195. Arsenic and trichloroethene (TCE) are two highly prevalent groundwater contaminants in the United States, and the presence of bioavailable arsenic is of particular concern at remediation sites in which reductive dechlorination has been employed. While we previously showed that low concentrations of arsenite (As(III)) inhibit the keystone TCE-reducing microorganism, D. mccartyi, this study reports the utilization of physiological analysis, transcriptomics, and metabolomics to assess the effects of arsenic on the metabolisms, gene expression, and nutrient exchanges in the described co-culture. It was found that the presence of D. vulgaris ameliorated arsenic stress on D. mccartyi, improving TCE dechlorination under arsenic-contaminated conditions. Nutrient and amino acid export by D. vulgaris may be a stress-ameliorating exchange in this syntrophic co-culture under arsenic stress, based on upregulation of transporters and increased extracellular nutrients like sarcosine and ornithine. These results broaden our knowledge of microbial community interactions and will support the further development and implementation of robust bioremediation strategies at multi-contaminant sites. more...
- Published
- 2023
11. Restricted intake of sulfur-containing amino acids reversed the hepatic injury induced by excess Desulfovibrio through gut–liver axis
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Lingxi Zhou, Gexue Lu, Yawen Nie, Yilin Ren, Jin-Song Shi, Yuzheng Xue, Zheng-Hong Xu, and Yan Geng
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Gut microbiota ,liver–gut axis ,Desulfovibrio ,purified diet ,bile acid metabolism ,sulfur-containing amino acids ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Diet is a key player in gut–liver axis. However, the effect of different dietary patterns on gut microbiota and liver functions remains unclear. Here, we used rodent standard chow and purified diet to mimic two common human dietary patterns: grain and plant-based diet and refined-food-based diet, respectively and explored their impacts on gut microbiota and liver. Gut microbiota experienced a great shift with notable increase in Desulfovibrio, gut bile acid (BA) levels elevated significantly, and liver inflammation was observed in mice fed with the purified diet. Liver inflammation and elevated gut BA levels also occurred in mice fed with the chow diet after receiving Desulfovibrio desulfuricans ATCC 29,577 (DSV). Restriction of sulfur-containing amino acids (SAAs) prevented liver injury mainly through higher hepatic antioxidant and detoxifying ability and reversed the elevated BA levels due to excess Desulfovibrio. Ex vivo fermentation of human fecal microbiota with primary BAs demonstrated that DSV enhanced production of secondary BAs. Higher concentration of both primary and secondary BAs were found in the gut of germ-free mice after receiving DSV. In conclusion, Restriction of SAAs in diet may become an effective dietary intervention to prevent liver injury associated with excess Desulfovibrio in the gut. more...
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- 2024
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12. How Often Should Microbial Contamination Be Detected in Aircraft Fuel Systems? An Experimental Test of Aluminum Alloy Corrosion Induced by Sulfate-Reducing Bacteria.
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Lu, Bochao, Zhang, Yimeng, Guo, Ding, Li, Yan, Zhang, Ruiyong, Cui, Ning, and Duan, Jizhou
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ALUMINUM alloys , *MICROBIOLOGICALLY influenced corrosion , *AIRCRAFT fuels , *FUEL systems , *PETROLEUM as fuel - Abstract
Microbial contamination in aircraft fuel-containing systems poses significant threats to flight safety and operational integrity as a result of microbiologically influenced corrosion (MIC). Regular monitoring for microbial contamination in these fuel systems is essential for mitigating MIC risks. However, the frequency of monitoring remains a challenge due to the complex environmental conditions encountered in fuel systems. To investigate the impact of environmental variables such as water content, oxygen levels, and temperature on the MIC of aluminum alloy in aircraft fuel systems, orthogonal experiments with various combinations of these variables were conducted in the presence of sulfate-reducing bacteria. Among these variables, water content in the fuel oil demonstrated the most substantial influence on the corrosion rate of aluminum alloys, surpassing the effects of oxygen and temperature. Notably, the corrosion rate of aluminum alloys was the highest in an environment characterized by a 1:1 water/oil ratio, 0% oxygen, and a temperature of 35 °C. Within this challenging environment, conducive to accelerated corrosion, changes in the corrosion behavior of aluminum alloys over time were analyzed to identify the time point at which MIC intensified. Observations revealed a marked increase in the depth and width of corrosion pits, as well as in the corrosion weight-loss rate, starting from the 7th day. These findings offer valuable insights for determining the optimal frequency of microbial contamination detection in aircraft fuel systems. [ABSTRACT FROM AUTHOR] more...
- Published
- 2024
- Full Text
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13. Magnesium Oxide Reduces Anxiety-like Behavior in Mice by Inhibiting Sulfate-Reducing Bacteria.
- Author
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Coffman, Cristina N., Carroll-Portillo, Amanda, Alcock, Joe, Singh, Sudha B., Rumsey, Kellin, Braun, Cody A., Xue, Bingye, and Lin, Henry C.
- Subjects
SULFATE-reducing bacteria ,CENTRAL nervous system ,ANXIETY ,GUT microbiome ,HYDROGEN sulfide - Abstract
The gut microbiota–brain axis allows for bidirectional communication between the microbes in our gastrointestinal (GI) tract and the central nervous system. Psychological stress has been known to disrupt the gut microbiome (dysbiosis) leading to anxiety-like behavior. Pathogens administered into the gut have been reported to cause anxiety. Whether commensal bacteria affect the gut–brain axis is not well understood. In this study, we examined the impact of a commensal sulfate-reducing bacteria (SRB) and its metabolite, hydrogen sulfide (H
2 S), on anxiety-like behavior. We found that mice gavaged with SRB had increased anxiety-like behavior as measured by the open field test. We also tested the effects of magnesium oxide (MgO) on SRB growth both in vitro and in vivo using a water avoidance stress (WAS) model. We found that MgO inhibited SRB growth and H2 S production in a dose-dependent fashion. Mice that underwent psychological stress using the WAS model were observed to have an overgrowth (bloom) of SRB (Deferribacterota) and increased anxiety-like behavior. However, WAS-induced overgrowth of SRB and anxiety-like behavioral effects were attenuated in animals fed a MgO-enriched diet. These findings supported a potential MgO-reversible relationship between WAS-induced SRB blooms and anxiety-like behavior. [ABSTRACT FROM AUTHOR] more...- Published
- 2024
- Full Text
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14. Lactobacillus acidophilus alleviates lung inflammation in LPS-induced acute pneumonia in mice: efficacy and mechanism
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XIE Langlang, WANG Zonghua, and WANG Wei
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acute pneumonia ,lactobacillus acidophilus ,lipopolysaccharide ,gut microbiota ,desulfovibrio ,Medicine (General) ,R5-920 - Abstract
Objective To investigate the effect of Lactobacillus acidophilus (L. acidophilus) on the inflammation of mice with lipopolysaccharide (LPS)-induced acute pneumonia and the possible mechanism. Methods Twenty-one mice were divided into a control group (NC, n=6), a model group (LPS, n=8) and a L. acidophilus-treated group (Probiotics, n=7). The mice in the control group were fed normally, while those in the LPS and Probiotics groups were treated with 10 mg/kg LPS through tracheal drip to establish a mouse model of acute pneumonia, and those in the Probiotics group were given L. acidophilus. All of the mice were executed in 7 d after modeling. The pathological changes of lung tissue were assessed by HE staining and wet/dry ratio (W/D) of lung tissue. ELISA was used to detect the levels of inflammatory factors in bronchoalveolar lavage fluid and the activity of myeloperoxidase (MPO) in lung tissue. 16S rDNA sequencing was employed to observe the diversity of intestinal flora, and immunohistochemical (IHC) staining was applied to determine the expression of ZO-1 and TRPV4. Results The Probiotics group had significantly reduced W/D ratio and IL-1β, TNF-α and IL-6 levels, increased IL-10 level (P < 0.05), diminished MPO activity (P < 0.05), down-regulated TRPV4 expression in colon and lung tissues, and upregulated ZO-1 expression in colon tissue, meanwhile, the abundance of Helicobacter spp. and Desulfovibrio spp. was significantly altered when compared with the LPS group. Conclusion L. acidophilus alleviates lung inflammation in LPS-induced pneumonia in mice, which may be associated with intestinal abundance of Desulfovibrio spp. in the, activity of TRPV4 channel and intestinal mucosal permeability. more...
- Published
- 2024
- Full Text
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15. Sulfate-Reducing Bacteria Isolated from an Oil Field in Kazakhstan and a Description of Pseudodesulfovibrio karagichevae sp. nov.
- Author
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Salimat K. Bidzhieva, Tatyana P. Tourova, Denis S. Grouzdev, Salima R. Samigullina, Diyana S. Sokolova, Andrey B. Poltaraus, Alexander N. Avtukh, Vera M. Tereshina, Andrey V. Mardanov, Nurlan S. Zhaparov, and Tamara N. Nazina more...
- Subjects
Pseudodesulfovibrio ,sulfate-reducing bacteria ,polyphasic taxonomy ,functional genomics ,unique genes ,petroleum reservoir ,Biology (General) ,QH301-705.5 - Abstract
Sulfidogenic bacteria cause numerous issues in the oil industry since they produce sulfide, corroding steel equipment, reducing oil quality, and worsening the environmental conditions in oil fields. The purpose of this work was to isolate and taxonomically identify the sulfidogenic bacteria responsible for the corrosion of steel equipment at the Karazhanbas oil field (Kazakhstan). In this study, we characterized five sulfidogenic strains of the genera Pseudodesulfovibrio, Oleidesulfovibrio, and Acetobacterium isolated from the formation water of the Karazhanbas oil field (Kazakhstan). Sulfate-reducing strain 9FUST revealed 98.9% similarity of the 16S rRNA gene sequence with the closely related strain ‘Pseudodesulfovibrio methanolicus’ 5S69T and was studied in detail to enhance the taxonomic resolution. Strain 9FUST grew optimally at 23–28 °C, pH 6.5, and 0–2% (w/v) NaCl. The strain used lactate, pyruvate, methanol, ethanol, fructose, ribose, and H2/CO2 (in the presence of acetate) as carbon and energy sources for sulfate reduction. Iso-C17:1 ω11, C15:0, iso-C15:0, and C16:0 were the predominant fatty acids. The genome is 4.20 Mbp with a G + C content of 64.0%. The average nucleotide identity and digital DNA–DNA hybridization values with Pseudodesulfovibrio spp. genomes were 72.5–91.6% (T (=VKM B-3654T = KCTC 25498T) belonged to a novel Pseudodesulfovibrio species, for which the name Pseudodesulfovibrio karagichevae sp. nov. is proposed. Pangenome analysis of sixteen Pseudodesulfovibrio species and functional annotation analysis of identified genes revealed complete modules of enzymes of the main metabolic pathways, characteristic of bacteria of this genus, and unique genes highlighting the adaptations of strain 9FUST in carbohydrate metabolism, nutrient uptake, and environmental stress response. Isolation of these strains expands our understanding of the diversity of sulfidogens in oil reservoirs and can be used to test the effectiveness of biocides used in an oil field. more...
- Published
- 2024
- Full Text
- View/download PDF
16. Population‐based metagenomics analysis reveals altered gut microbiome in sarcopenia: data from the Xiangya Sarcopenia Study
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Wang, Yilun, Zhang, Yuqing, Lane, Nancy E, Wu, Jing, Yang, Tuo, Li, Jiatian, He, Hongyi, Wei, Jie, Zeng, Chao, and Lei, Guanghua
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Biomedical and Clinical Sciences ,Allied Health and Rehabilitation Science ,Health Sciences ,Clinical Sciences ,Sports Science and Exercise ,Digestive Diseases ,Genetics ,Bacteroides ,Clostridiaceae ,Clostridiales ,Desulfovibrio ,Female ,Furaldehyde ,Gastrointestinal Microbiome ,Humans ,Middle Aged ,Phenylalanine ,RNA ,Ribosomal ,16S ,Sarcopenia ,Staurosporine ,Tryptophan ,Tyrosine ,alpha-Linolenic Acid ,Gut microbiome ,Metagenomics ,Population-based study ,Physiology ,Human Movement and Sports Sciences ,Clinical sciences ,Allied health and rehabilitation science ,Sports science and exercise - Abstract
BackgroundSeveral studies have examined gut microbiota and sarcopenia using 16S ribosomal RNA amplicon sequencing; however, this technique may not be able to identify altered specific species and functional capacities of the microbes. We performed shotgun metagenomic sequencing to compare the gut microbiome composition and function between individuals with and without sarcopenia.MethodsParticipants were from a community-based observational study conducted among the residents of rural areas in China. Appendicular skeletal muscle mass was assessed using direct segmental multi-frequency bioelectrical impedance and grip strength using a Jamar Hydraulic Hand dynamometer. Physical performance was evaluated using the Short Physical Performance Battery, 5-time chair stand test and gait speed with the 6 m walk test. Sarcopenia and its severity were diagnosed according to the Asian Working Group for Sarcopenia 2019 algorithm. The gut microbiome was profiled by shotgun metagenomic sequencing to determine the microbial composition and function. A gut microbiota-based model for classification of sarcopenia was constructed using the random forest model, and its performance was assessed using the area under receiver-operating characteristic curve (AUC).ResultsThe study sample included 1417 participants (women: 58.9%; mean age: 63.3 years; sarcopenia prevalence: 10.0%). β-diversity indicated by Bray-Curtis distance (genetic level: P = 0.004; taxonomic level of species: P = 0.020), but not α-diversity indicated by Shannon index (genetic level: P = 0.962; taxonomic level of species: P = 0.922), was significantly associated with prevalent sarcopenia. After adjusting for potential confounders, participants with sarcopenia had higher relative abundance of Desulfovibrio piger (P = 0.003, Q = 0.090), Clostridium symbiosum (P more...
- Published
- 2022
17. Insight into the Inhibition of the Poisonous Sulfide Production from Sulfate-Reducing Microbiota in Mariculture Habitat.
- Author
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Zhao, Xuning, Zhang, Zhiming, Zhao, Yangguo, and Mupindu, Progress
- Abstract
The production of toxic sulfides is a common environmental problem in mariculture. Therefore, the effective inhibition of sulfidogens is the key to prevent sulfides production. In this study, the possibility and mechanism of nitrate (NO
3 − ) inhibiting the activity of the sulfate-reducing microbiota (SRM) from mariculture sediments was investigated. The results showed that 1, 3, and 5 mmol L−1 NO3 − continuously inhibited sulfide production for 1–3 d. As NO3 − dosage increased to 7 mmol L−1 , the duration of inhibition increased to 6 days. Denitrifying product NO2 − heavily inhibited the activity of dissimilar sulfate reductase gene (dsrB) by 3 orders, which was the main reason that the sulfate-reducing activity was inhibited. The SRM structure changed significantly with the dosage of NO3 − , while the abundance of sulfidogens Desulfovibrio species increased due to their capability of detoxifying nitrite through nitrite reductase. Hence, sulfidogens Desulfovibrio species are more adaptable to a high nitrate/nitrite environment, and the traditional control strategies by dosing nitrate/nitrite should be paid more attention to. The findings will serve as helpful guidelines for sulfate-reducing microbiota in the habitat of mariculture to reduce their generation of poisonous sulfide. [ABSTRACT FROM AUTHOR] more...- Published
- 2024
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18. Distinct gene clusters drive formation of ferrosome organelles in bacteria.
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Grant, Carly, Amor, Matthieu, Trujillo, Hector, Krishnapura, Sunaya, Iavarone, Anthony, and Komeili, Arash
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Bacterial Proteins ,Desulfovibrio ,Ferric Compounds ,Gram-Negative Bacteria ,Multigene Family ,Organelles ,Phylogeny ,Proteomics ,Rhodopseudomonas ,Shewanella putrefaciens - Abstract
Cellular iron homeostasis is vital and maintained through tight regulation of iron import, efflux, storage and detoxification1-3. The most common modes of iron storage use proteinaceous compartments, such as ferritins and related proteins4,5. Although lipid-bounded iron compartments have also been described, the basis for their formation and function remains unknown6,7. Here we focus on one such compartment, herein named the ferrosome, that was previously observed in the anaerobic bacterium Desulfovibrio magneticus6. Using a proteomic approach, we identify three ferrosome-associated (Fez) proteins that are responsible for forming ferrosomes in D. magneticus. Fez proteins are encoded in a putative operon and include FezB, a P1B-6-ATPase found in phylogenetically and metabolically diverse species of bacteria and archaea. We show that two other bacterial species, Rhodopseudomonas palustris and Shewanella putrefaciens, make ferrosomes through the action of their six-gene fez operon. Additionally, we find that fez operons are sufficient for ferrosome formation in foreign hosts. Using S. putrefaciens as a model, we show that ferrosomes probably have a role in the anaerobic adaptation to iron starvation. Overall, this work establishes ferrosomes as a new class of iron storage organelles and sets the stage for studying their formation and structure in diverse microorganisms. more...
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- 2022
19. The Role of Akkermansia muciniphila on Improving Gut and Metabolic Health Modulation: A Meta-Analysis of Preclinical Mouse Model Studies
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Leila Khalili, Gwoncheol Park, Ravinder Nagpal, and Gloria Salazar
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Akkermansia muciniphila ,Desulfovibrio ,Family XIII AD3011 group ,Candidatus Saccharimonas ,gut ,metabolic health ,Biology (General) ,QH301-705.5 - Abstract
Akkermansia muciniphila (A. muciniphila) and its derivatives, including extracellular vesicles (EVs) and outer membrane proteins, are recognized for enhancing intestinal balance and metabolic health. However, the mechanisms of Akkermansia muciniphila’s action and its effects on the microbiome are not well understood. In this study, we examined the influence of A. muciniphila and its derivatives on gastrointestinal (GI) and metabolic disorders through a meta-analysis of studies conducted on mouse models. A total of 39 eligible studies were identified through targeted searches on PubMed, Web of Science, Science Direct, and Embase until May 2024. A. muciniphila (alive or heat-killed) and its derivatives positively affected systemic and gut inflammation, liver enzyme level, glycemic response, and lipid profiles. The intervention increased the expression of tight-junction proteins in the gut, improving gut permeability in mouse models of GI and metabolic disorders. Regarding body weight, A. muciniphila and its derivatives prevented weight loss in animals with GI disorders while reducing body weight in mice with metabolic disorders. Sub-group analysis indicated that live bacteria had a more substantial effect on most analyzed biomarkers. Gut microbiome analysis using live A. muciniphila identified a co-occurrence cluster, including Desulfovibrio, Family XIII AD3011 group, and Candidatus Saccharimonas. Thus, enhancing the intestinal abundance of A. muciniphila and its gut microbial clusters may provide more robust health benefits for cardiometabolic, and age-related diseases compared with A. muciniphila alone. The mechanistic insight elucidated here will pave the way for further exploration and potential translational applications in human health. more...
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- 2024
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20. Desulfovibrio desulfuricans and its derived metabolites confer resistance to FOLFOX through METTL3Research in context
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Guifang Li, Huan Liu, Yangmeng Yu, Qian Wang, Chen Yang, Yang Yan, Fang Wang, and Yong Mao
- Subjects
Colorectal cancer ,FOLFOX ,Desulfovibrio ,S-adenosylmethionine ,Methyltransferase-like 3 ,Medicine ,Medicine (General) ,R5-920 - Abstract
Summary: Background: Chemoresistance is a critical factor contributing to poor prognosis in clinical patients with cancer undergoing postoperative adjuvant chemotherapy. The role of gut microbiota in mediating resistance to tumour chemotherapy remains to be investigated. Methods: Patients with CRC were categorised into clinical benefit responders (CBR) and no clinical benefit responders (NCB) based on chemotherapy efficacy. Differential bacterial analysis using 16S rRNA sequencing revealed Desulfovibrio as a distinct microbe between the two groups. Employing a syngeneic transplantation model, we assessed the effect of Desulfovibrio on chemotherapy by measuring tumour burden, weight, and Ki-67 expression. We further explored the mechanisms underlying the compromised chemotherapeutic efficacy of Desulfovibrio using metabolomics, western blotting, colony formation, and cell apoptosis assays. Findings: In comparison, Desulfovibrio was more abundant in the NCB group. In vivo experiments revealed that Desulfovibrio colonisation in the gut weakened the efficacy of FOLFOX. Treatment with Desulfovibrio desulfuricans elevates serum S-adenosylmethionine (SAM) levels. Interestingly, SAM reduced the sensitivity of CRC cells to FOLFOX, thereby promoting the growth of CRC tumours. These experiments suggest that SAM promotes the growth and metastasis of CRC by driving the expression of methyltransferase-like 3 (METTL3). Interpretation: A high abundance of Desulfovibrio in the intestines indicates poor therapeutic outcomes for postoperative neoadjuvant FOLFOX chemotherapy in CRC. Desulfovibrio drives the manifestation of METTL3 in CRC, promoting resistance to FOLFOX chemotherapy by increasing the concentration of SAM. Funding: This study is supported by Wuxi City Social Development Science and Technology Demonstration Project (N20201005). more...
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- 2024
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21. Moringa oleifera leaf alleviates functional constipation via regulating the gut microbiota and the enteric nervous system in mice.
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Xiaoyu Gao, Weiqian Yang, Sijin Li, Shuangfeng Liu, Weixing Yang, Shuang Song, Jun Sheng, Yan Zhao, and Yang Tian
- Abstract
Moringa oleifera Lam. leaf is not only a new food resource in China, but also a traditional medicinal plant. It is commonly used in the folk to alleviate constipation, but its laxative mechanism is not fully understood. Hence we investigated it in loperamide-induced functional constipation (FC) mice. The results showed that MOAE significantly regulated not only gastrointestinal hormones and neurotransmitters in serum but also important gastrointestinal motility factors in the enteric nervous system (ENS)-interstitial cells of Cajal (ICCs)-smooth muscle cell (SMC) network. Meanwhile, MOAE attenuated intestinal inflammation, increased cecal short-chain fatty acid levels and colonic antimicrobial peptide expression, and improved the impaired intestinal barrier function in loperamide-induced FC mice. In addition, MOAE also increased fecal water content by inhibiting the mRNA expression of colonic aquaporins (Aqp3 and Aqp4) in FC mice. Interestingly and importantly, MOAE affected the intestinal microbiota by inhibiting some key “constipation-causing” microbiota, such as Bacteroidaceae, Clostridiaceae, Bacteroides, and Ruminococcus, and promoting the growth of other important “constipation-curing” microbiota, such as Butyricoccus, Tyzzerella, and Desulfovibrio. These important taxa are significantly associated with a variety of indicators of constipation. These findings suggest that MOAE can promote defecation through its rich chemical composition to modulate the ENS-ICCs-SMCs network and the gut microecosystem. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
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22. Low-Abundance Sulfidogenic Bacteria Carry out Intensive Sulfate Reduction in Terrestrial Hydrotherms of the Barguzin Valley.
- Author
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Rusanov, I. I., Kadnikov, V. V., Lukina, A. P., Danilova, E. V., Sokolyanskaya, L. O., Ravin, N. V., and Karnachuk, O. V.
- Subjects
- *
SULFATES , *BACTERIA , *RIBOSOMAL RNA , *PROKARYOTES , *SEDIMENTS - Abstract
Preliminary microbiological investigation of the Barguzin Valley terrestrial hydrotherms did not elucidate the composition of the community of sulfate-reducing prokaryotes (SRP) responsible for high sulfide concentrations in the Kuchiger and Umkhei springs. Measurement of sulfate reduction rate (SRR) with labeled sulfate in the samples collected together with those for determination of community composition by 16S rRNA gene profiling revealed that, while not numerous, the SRP consisting of Desulfobacterota and Thermodesulfovibrio was responsible for a highly active process. SRR in the sediments of the Umkhei and Kuchiger springs was up to 12.7 ± 0.2 and 2.05 ± 0.08 mg S dm–3 day–1, respectively. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
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23. Fecal microbiota as a predictor of acupuncture responses in patients with postpartum depressive disorder.
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Yu-Mei Zhou, Jin-Jun Yuan, Yu-Qin Xu, Yan-Hua Gou, Zhu, Yannas Y. X., Chen Chen, Xing-Xian Huang, Xiao-Ming Ma, Min-Pi, and Zhuo-Xin Yang
- Subjects
MENTAL depression ,CALPROTECTIN ,HAMILTON Depression Inventory ,ACUPUNCTURE ,GUT microbiome ,RIBOSOMAL RNA - Abstract
Background: There are several clinical and molecular predictors of responses to antidepressant therapy. However, these markers are either too subjective or complex for clinical use. The gut microbiota could provide an easily accessible set of biomarkers to predict therapeutic efficacy, but its value in predicting therapy responses to acupuncture in patients with depression is unknown. Here we analyzed the predictive value of the gut microbiota in patients with postpartum depressive disorder (PPD) treated with acupuncture. Methods: Seventy-nine PPD patients were enrolled: 55 were treated with acupuncture and 24 did not received any treatment. The 17-item Hamilton depression rating scale (HAMD-17) was used to assess patients at baseline and after eight weeks. Patients receiving acupuncture treatment were divided into an acupuncture-responsive group or non-responsive group according to HAMD-17 scores changes. Baseline fecal samples were obtained from the patients receiving acupuncture and were analyzed by high-throughput 16S ribosomal RNA sequencing to characterize the gut microbiome. Results: 47.27% patients responded to acupuncture treatment and 12.5% patients with no treatment recovered after 8-week follow-up. There was no significant difference in α-diversity between responders and non-responders. The β-diversity of non-responders was significantly higher than responders. Paraprevotella and Desulfovibrio spp. were significantly enriched in acupuncture responders, and these organisms had an area under the curve of 0.76 and 0.66 for predicting responder patients, respectively. Conclusions: Paraprevotella and Desulfovibrioare may be useful predictive biomarkers to predict PPD patients likely to respond to acupuncture. Larger studies and validation in independent cohorts are now needed to validate our findings. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
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24. Significance of lysogeny for the metabolism of Desulfovibrio spp. strains isolated from aquatic environments of Georgia
- Author
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Balarjishvili, N, Kvachadze, L, Tevdoradze, E, Skhirtladze, N, Leshkasheli, L, Bolkvadze, D, Pataridze, T, Meskhi, T, Chakraborty, R, Kutateladze, M, and Torok, T
- Subjects
Microbiology ,Biological Sciences ,2.2 Factors relating to the physical environment ,Aquatic Organisms ,Bacteriophages ,Desulfovibrio ,Georgia ,Lysogeny ,Seawater ,Sulfates ,Water Microbiology ,bacteriophages ,ecology ,environmental ,identification ,water ,Agricultural ,veterinary and food sciences ,Biological sciences ,Biomedical and clinical sciences - Abstract
AimsSulphate-reducing bacteria (SRB) are ecologically important group of anaerobic micro-organisms that can reduce sulphate to form hydrogen sulphide-a toxic gas causing iron corrosion on metal surfaces. In this work, SRB strains were isolated from aquatic environments in the country of Georgia to determine their lysogenicity and the role of temperate phages in host metabolism.Methods and resultsSRB strains were isolated in samples from the Black Sea coast of Georgia. Based on their genetic, cytological and physiological properties of bacteria, 10 Georgian isolates were assigned to the genus Desulfovibrio. Temperate bacteriophages were induced from three out of ten strains by UV-exposure. Comparison of metal (Fe and Cr) reduction and utilization of various carbon sources by the wild-type (lysogenic) bacterial strains and their UV-irradiated counterparts was done.ConclusionsTemperate phage in the cells of SRB could alter significant functions of bacteria and may have a contribution in the acquisition of different traits by SRB.Significance and impact of the studyThis article pointed to a significant role for temperate bacteriophages in the metabolism and metabolic potential of host strains of SRB, which were first isolated from the aquatic environment of Georgia. more...
- Published
- 2021
25. Features of the Intestinal Microbiota in Patients with Inflammatory Intestinal Diseases
- Author
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S. N. Lagutina, P. A. Chizhkov, A. A. Zuikova, E. Yu. Esina, I. S. Dobrynina, O. S. Skuratova, and M. Yu. Syromyatnikov
- Subjects
inflammatory bowel diseases ,ulcerative colitis ,sequencing ,gut microbiota ,taxonomic diversity ,eubiosis ,pathogenic cluster ,bacteroidetes ,firmicutes ,desulfovibrio ,methanobrevibacter ,Science ,Medicine ,History of scholarship and learning. The humanities ,AZ20-999 - Abstract
Introduction. Inflammatory bowel diseases (IBD) occupy a leading position in the structure of diseases of the gastrointestinal tract (GIT), as they are a progressive chronic pathology with an autoimmune type of inflammation. Changes in the composition of the gut microbiota can determine morphological changes at the latent stage of the disease. Aim of the study. To assess the genus-species biodiversity of the microbiota in patients with IBD. Patients and methods. 16S rRNA sequencing of the intestinal microbiota was performed in 15 patients with confirmed ulcerative colitis (UC) and 20 healthy controls. The parameters of the full blood count and the serum level of C-reactive protein (CRP) were analyzed. The analysis of the obtained data was carried out using Microsoft Excel and Statistica software. Results. Among the studied laboratory parameters in patients with IBD, there was a significant increase in CRP, leukocyte and neutrophil counts compared to the control group. Sequencing of the gut microbiota showed a decrease in the normobiota, as well as an increase in the representatives of the pathogenic cluster. Conclusion. In the present study, we demonstrated a decrease in the biodiversity of the gut microbiota in patients with IBD compared to the control group, a significant change in the pathogenic cluster, and an imbalance between the representatives of Bacteroidetes and Firmicutes. more...
- Published
- 2023
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26. Magnesium Oxide Reduces Anxiety-like Behavior in Mice by Inhibiting Sulfate-Reducing Bacteria
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Cristina N. Coffman, Amanda Carroll-Portillo, Joe Alcock, Sudha B. Singh, Kellin Rumsey, Cody A. Braun, Bingye Xue, and Henry C. Lin
- Subjects
sulfate-reducing bacteria (SRB) ,Desulfovibrio ,Deferribacterota ,dysbiosis ,hydrogen sulfide (H2S) ,Biology (General) ,QH301-705.5 - Abstract
The gut microbiota–brain axis allows for bidirectional communication between the microbes in our gastrointestinal (GI) tract and the central nervous system. Psychological stress has been known to disrupt the gut microbiome (dysbiosis) leading to anxiety-like behavior. Pathogens administered into the gut have been reported to cause anxiety. Whether commensal bacteria affect the gut–brain axis is not well understood. In this study, we examined the impact of a commensal sulfate-reducing bacteria (SRB) and its metabolite, hydrogen sulfide (H2S), on anxiety-like behavior. We found that mice gavaged with SRB had increased anxiety-like behavior as measured by the open field test. We also tested the effects of magnesium oxide (MgO) on SRB growth both in vitro and in vivo using a water avoidance stress (WAS) model. We found that MgO inhibited SRB growth and H2S production in a dose-dependent fashion. Mice that underwent psychological stress using the WAS model were observed to have an overgrowth (bloom) of SRB (Deferribacterota) and increased anxiety-like behavior. However, WAS-induced overgrowth of SRB and anxiety-like behavioral effects were attenuated in animals fed a MgO-enriched diet. These findings supported a potential MgO-reversible relationship between WAS-induced SRB blooms and anxiety-like behavior. more...
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- 2024
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27. Bloodstream co-infection with Turicibacter sanguinis and Desulfovibrio desulfuricans in a patient with a flare of ulcerative colitis – A case report and review of the literature
- Author
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Kendall Kling, Vivek Paul, Teresa Zembower, and Chao Qi
- Subjects
Desulfovibrio ,Turicibacter sanguinis ,Ulcerative colitis ,Infectious and parasitic diseases ,RC109-216 - Abstract
Turicibacter sanguinis was isolated in 2002 from the blood of a patient with appendicitis. We report a bacteremia with T. sanguinis and Desulfovibrio desulfuricans in a patient with ulcerative colitis. T. sanguinis grew in thioglycolate media and identification was confirmed with 16S rRNA sequencing. more...
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- 2024
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28. Coexistence of Psychrophilic, Mesophilic, and Thermophilic Sulfate-Reducing Bacteria in a Deep Subsurface Aquifer Associated with Coal-Bed Methane Production.
- Author
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Karnachuk, Olga V., Panova, Inna A., Rusanov, Igor I., Schetinina, Lilia, Lepokurova, Olesya Y., Domrocheva, Evgenia V., Kadnikov, Vitaly V., Avakyan, Marat R., Lukina, Anstasia P., Glukhova, Liubov B., Pimenov, Nikolai V., and Ravin, Nikolai V. more...
- Subjects
- *
SULFATE-reducing bacteria , *THERMOPHILIC bacteria , *GAS wells , *COALBED methane , *RADIOACTIVE tracers , *COAL basins - Abstract
The microbial community of subsurface environments remains understudied due to limited access to deep strata and aquifers. Coal-bed methane (CBM) production is associated with a large number of wells pumping water out of coal seams. CBM wells provide access to deep biotopes associated with coal-bed water. Temperature is one of the key constraints for the distribution and activity of subsurface microorganisms, including sulfate-reducing prokaryotes (SRP). The 16S rRNA gene amplicon sequencing coupled with in situ sulfate reduction rate (SRR) measurements with a radioactive tracer and cultivation at various temperatures revealed that the SRP community of the coal bed water of the Kuzbass coal basin is characterized by an overlapping mesophilic-psychrophilic boundary. The genus Desulfovibrio comprised a significant share of the SRP community. The D. psychrotolerans strain 1203, which has a growth optimum below 20 °C, dominated the cultivated SRP. SRR in coal bed water varied from 0.154 ± 0.07 to 2.04 ± 0.048 nmol S cm−3 day−1. Despite the ambient water temperature of ~ 10–20 °C, an active thermophilic SRP community occurred in the fracture water, which reduced sulfate with the rate of 0.159 ± 0.023 to 0.198 ± 0.007 nmol S cm−3 day−1 at 55 °C. A novel moderately thermophilic "Desulforudis audaxviator"-clade SRP has been isolated in pure culture from the coal-bed water. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
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29. Study on the treatment of sulfite wastewater by Desulfovibrio.
- Author
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Zhao, Baofu, Sun, Hui, Jiang, Peng, Rizwan, Muhammad, Zhou, Mengke, and Zhou, Xiaolong
- Abstract
In the wet flue gas desulfurization (WFGD) process, SO
2 is adsorbed by alkaline liquor to produce alkaline wastewater containing sulfate and sulfite. Although the traditional chemical treatment method can achieve a high removal rate, it consumes a large number of chemicals and yields a large number of low-value by-products. The biological treatment process is a greener and more environmentally friendly treatment method. The current work studies microbial flue gas desulfurization directly using sulfite as the electron acceptor in the reduction process. Desulfovibrio were obtained by isolation and purification, and their growth conditions in sulfite wastewater and desulfurization process conditions were investigated by intermittent and continuous experiments. The results of intermittent experiments indicated that the optimal growth conditions of Desulfovibrio were a temperature of 38 °C, a pH value of 8.0, a COD/SO3 2− of 2 and that the growth of bacteria would be inhibited at a pH above 9.0 or below 7.3. Furthermore, Desulfovibrio could grow in simulated wastewater with a high SO3 2− concentration of 8000 mg/L. The results of continuous experiments showed that the removal of sulfite and the recovery of elemental sulfur was realized by a micro-oxygen depletion process, and the removal rate of sulfite of 99%, the yield of elemental sulfur is more than 80% and can reach 90% under the condition of low influent concentration. The bacteria grew well at a temperature of 40 °C and a pH value of the influent water of 7.5. To ensure the treatment effect, the hydraulic retention time (HRT) should be more than doubled for each 1000 mg/L increase in the influent sulfite concentration under the same reflux ratio. When the influent sulfite concentration was 1000 mg/L, 2000 mg/L, 3000 mg/L, and 4000 mg/L, the corresponding HRT was 3.01 h, 6.94 h, 17.4 h, and 31.9 h, respectively. The dominant species in the reactor was Desulfovibrio bacteria at 63.9% abundance. This study demonstrated the feasibility of using sulfite as an electron acceptor for microbial desulfurization, which can optimize the initial process and provide the possibility of treating high-concentration sulfite wastewater. [ABSTRACT FROM AUTHOR] more...- Published
- 2023
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30. A Comparative Multi-Frequency EPR Study of Dipolar Interaction in Tetra-Heme Cytochromes.
- Author
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Hagen, Wilfred R. and Louro, Ricardo O.
- Subjects
- *
CYTOCHROMES , *SHEWANELLA oneidensis , *CYTOCHROME c , *CHARGE exchange , *DIPOLE-dipole interactions , *HEME , *DATA analysis - Abstract
Distances between Fe ions in multiheme cytochromes are sufficiently short to make the intramolecular dipole-dipole interaction between hemes probable. In the analysis of EPR data from cytochromes, this interaction has thus far been ignored under the assumption that spectra are the simple sum of non-interacting components. Here, we use a recently developed low-frequency broadband EPR spectrometer to establish the extent of dipolar interaction in the example cytochromes, characterize its spectral signatures, and identify present limitations in the analysis. Broadband EPR spectra of Shewanella oneidensis MR-1 small tetraheme cytochrome (STC) have been collected over the frequency range of 0.45 to 13.11 GHz, and they have been compared to similar data from Desulfovibrio vulgaris Hildenborough cytochrome c3. The two cases are representative examples of two very different heme topologies and corresponding electron-transfer properties in tetraheme proteins. While in cytochrome c3, the six Fe-Fe distances can be sorted into two well-separated groups, those in STC are diffuse. Since the onset of dipolar interaction between Fe-Fe pairs is already observed in the X-band, the g values are determined in the simulation of the 13.11 GHz spectrum. Low-frequency spectra are analyzed with the inclusion of dipolar interaction based on available structural data on mutual distances and orientations between all hemes. In this procedure, all 24 possible assignments of individual heme spectra to heme topologies are sampled. The 24 configurations can be reduced to a few, but inspection falls short of a unique assignment, due to a remaining lack of understanding of the fine details of these complex spectra. In general, the EPR analysis suggests the four-heme system in c3 to be more rigid than that in STC, which is proposed to be related to different physiological roles in electron transfer. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
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31. Experimental evolution reveals nitrate tolerance mechanisms in Desulfovibrio vulgaris.
- Author
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Wu, Bo, Liu, Feifei, Zhou, Aifen, Li, Juan, Shu, Longfei, Kempher, Megan L, Yang, Xueqin, Ning, Daliang, Pan, Feiyan, Zane, Grant M, Wall, Judy D, Van Nostrand, Joy D, Juneau, Philippe, Chen, Shouwen, Yan, Qingyun, Zhou, Jizhong, and He, Zhili more...
- Subjects
Desulfovibrio ,Desulfovibrio vulgaris ,Nitrates ,Sulfates ,Nitrogen Oxides ,Oxidation-Reduction ,Genotype ,Microbiology ,Environmental Sciences ,Biological Sciences ,Technology - Abstract
Elevated nitrate in the environment inhibits sulfate reduction by important microorganisms of sulfate-reducing bacteria (SRB). Several SRB may respire nitrate to survive under elevated nitrate, but how SRB that lack nitrate reductase survive to elevated nitrate remains elusive. To understand nitrate adaptation mechanisms, we evolved 12 populations of a model SRB (i.e., Desulfovibrio vulgaris Hildenborough, DvH) under elevated NaNO3 for 1000 generations, analyzed growth and acquired mutations, and linked their genotypes with phenotypes. Nitrate-evolved (EN) populations significantly (p more...
- Published
- 2020
32. Magnetic genes: Studying the genetics of biomineralization in magnetotactic bacteria.
- Author
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McCausland, Hayley and KOMEILI, Arash
- Subjects
Bacterial Proteins ,Biomineralization ,DNA Transposable Elements ,Desulfovibrio ,Ferrosoferric Oxide ,Genes ,Bacterial ,Magnetosomes ,Magnetospirillum ,Metal Nanoparticles ,Mutagenesis ,Mutation - Abstract
Many species of bacteria can manufacture materials on a finer scale than those that are synthetically made. These products are often produced within intracellular compartments that bear many hallmarks of eukaryotic organelles. One unique and elegant group of organisms is at the forefront of studies into the mechanisms of organelle formation and biomineralization. Magnetotactic bacteria (MTB) produce organelles called magnetosomes that contain nanocrystals of magnetic material, and understanding the molecular mechanisms behind magnetosome formation and biomineralization is a rich area of study. In this Review, we focus on the genetics behind the formation of magnetosomes and biomineralization. We cover the history of genetic discoveries in MTB and key insights that have been found in recent years and provide a perspective on the future of genetic studies in MTB. more...
- Published
- 2020
33. Sulfate-Reducing Bacteria in Patients Undergoing Fixed Orthodontic Treatment
- Author
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Umarevathi Gopalakrishnan, Rajalakshmi Thakshina Murthy, A. Sumathi Felicita, Abdulrahman Alshehri, Wael Awadh, Abdullah Almalki, Thilla Sekar Vinothkumar, Hosam Ali Baeshen, Shilpa Bhandi, Arvind Kathir, Abhinaya Samala, A. Thirumal Raj, Artak Heboyan, and Shankargouda Patil more...
- Subjects
Desulfovibrio ,Microbial corrosion ,Nickel-titanium ,Orthodontics ,Periodontal disease ,Sulfate-reducing bacteria ,Dentistry ,RK1-715 - Abstract
Objectives: The oral microbiological environment may be implicated in the corrosion of orthodontic metals. This study aimed to examine the prevalence of sulfate-reducing bacteria (SRB) in orthodontic patients undergoing fixed appliance treatment. Methods: Sixty-nine orthodontic and 69 healthy non-orthodontic participants were enrolled in the study. Supragingival and subgingivaloral biofilm were collected and tested for the presence of SRB. The DNA extraction, polymerase chain reaction (PCR), and 16sRNA Sanger sequencing method was performed from the SRB-positive samples. The sequenced PCR products were analysed and compared with databases to identify the bacterial genus. Results: Amongst 69 orthodontic patients, characteristic black precipitates developed in 14, indicating the presence of iron sulfides which demonstrates the likelihood of SRB. Alternatively, 2 out of 69 showed the presence of SRB in healthy non-orthodontic participants (controls). Desulfovibrio spp was confirmed by analyses of 16sRNA sequencing, which revealed that the SRB prevalence was 20% in the examined participants with orthodontic appliances. Conclusions: The prevalence of SRB was found to be significantly higher amongst orthodontic patients compared to non-orthodontic participants. Presence of stainless steel in the oral environment may have facilitated the colonisation of SRB. more...
- Published
- 2023
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34. Microbiota-host crosstalk in the newborn and adult rumen at single-cell resolution
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Jia-Jin Wu, Senlin Zhu, Yi-Fan Tang, Fengfei Gu, Jian-Xin Liu, and Hui-Zeng Sun
- Subjects
Rumen ,Host single-cell transcriptome ,Epithelial microbiota ,Epithelial metabolome ,Microbiota-host crosstalk ,Desulfovibrio ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background The rumen is the hallmark organ of ruminants, playing a vital role in their nutrition and providing products for humans. In newborn suckling ruminants milk bypasses the rumen, while in adults this first chamber of the forestomach has developed to become the principal site of microbial fermentation of plant fibers. With the advent of single-cell transcriptomics, it is now possible to study the underlying cell composition of rumen tissues and investigate how this relates the development of mutualistic symbiosis between the rumen and its epithelium-attached microbes. Results We constructed a comprehensive cell landscape of the rumen epithelium, based on single-cell RNA sequencing of 49,689 high-quality single cells from newborn and adult rumen tissues. Our single-cell analysis identified six immune cell subtypes and seventeen non-immune cell subtypes of the rumen. On performing cross-species analysis of orthologous genes expressed in epithelial cells of cattle rumen and the human stomach and skin, we observed that the species difference overrides any cross-species cell-type similarity. Comparing adult with newborn cattle samples, we found fewer epithelial cell subtypes and more abundant immune cells, dominated by T helper type 17 cells in the rumen tissue of adult cattle. In newborns, there were more fibroblasts and myofibroblasts, an IGFBP3 + epithelial cell subtype not seen in adults, while dendritic cells were the most prevalent immune cell subtype. Metabolism-related functions and the oxidation-reduction process were significantly upregulated in adult rumen epithelial cells. Using 16S rDNA sequencing, fluorescence in situ hybridization, and absolute quantitative real-time PCR, we found that epithelial Desulfovibrio was significantly enriched in the adult cattle. Integrating the microbiome and metabolome analysis of rumen tissues revealed a high co-occurrence probability of Desulfovibrio with pyridoxal in the adult cattle compared with newborn ones while the scRNA-seq data indicated a stronger ability of pyroxidal binding in the adult rumen epithelial cell subtypes. These findings indicate that Desulfovibrio and pyridoxal likely play important roles in maintaining redox balance in the adult rumen. Conclusions Our integrated multi-omics analysis provides novel insights into rumen development and function and may facilitate the future precision improvement of rumen function and milk/meat production in cattle. more...
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- 2022
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35. Desulfovibrio in the Gut: The Enemy within?
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Singh, Sudha B., Carroll-Portillo, Amanda, and Lin, Henry C.
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GASTROINTESTINAL system ,SULFATE-reducing bacteria ,PATHOLOGY ,INTESTINAL diseases ,INFLAMMATORY bowel diseases - Abstract
Desulfovibrio (DSV) are sulfate-reducing bacteria (SRB) that are ubiquitously present in the environment and as resident commensal bacteria within the human gastrointestinal tract. Though they are minor residents of the healthy gut, DSV are opportunistic pathobionts that may overgrow in the setting of various intestinal and extra-intestinal diseases. An increasing number of studies have demonstrated a positive correlation between DSV overgrowth (bloom) and various human diseases. While the relationship between DSV bloom and disease pathology has not been clearly established, mounting evidence suggests a causal role for these bacteria in disease development. As DSV are the most predominant genera of SRB in the gut, this review summarizes current knowledge regarding the relationship between DSV and a variety of diseases. In this study, we also discuss the mechanisms by which these bacteria may contribute to disease pathology. [ABSTRACT FROM AUTHOR] more...
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- 2023
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36. Anaerobic Thermodesulfovibrio and Aerobic Meiothermus Coexist in Deep Thermal Water.
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Lukina, A. P., Kadnikov, V. V., Rusanov, I. I., Avakyan, M. R., Beletsky, A. V., Mardanov, A. V., Pimenov, N. V., Ravin, N. V., and Karnachuk, O. V.
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GEOTHERMAL resources , *SUPEROXIDE dismutase , *MICROBIAL mats , *ANAEROBIC bacteria , *AEROBIC bacteria , *AEROBIC metabolism , *PEROXIDASE , *UBIQUINONES , *CYTOCHROME c - Abstract
Research on the microorganisms inhabiting deep aquifers is based on sampling the water released from deep wells and is seldom concerned with the physicochemical processes of the water-rock system. The issue of metabolism of aerobic prokaryotes revealed in deep habitats by molecular techniques remains unclear. Cultivation is required for direct determination of relation of prokaryotes to oxygen. In the present work, aerobic and anaerobic bacteria, which were revealed in thermal radon baths of the Belokurikha resort by molecular techniques, were isolated. Profiling by the 16S rRNA gene revealed predominance of members of the Deionococcus-Thermus group belonging to the genus Meiothermus (17.6% reads) and considered strictly aerobic. Anaerobic sulfate-reducing Thermodesulfovibrio were also present in the sample. The habitat was characterized by reductive, alkaline conditions. Target-oriented cultivation revealed aerobically growing Meiothermus sp. 1165, which was closely related to Meiothermus cerbereus. An alkaliphilic anaerobic sulfate reducer Thermodesulfovibrio sp. 1176 was also isolated. The rate of sulfate reduction measured in the Belokurikha water using yielded the value of 41.4 ± 1.06 µg Sred L–1 day–1, or 1.29 nmol S mL–1 day–1. Analysis of the genome of strain 1176 revealed the presence of various mechanisms responsible for its relative resistance to oxygen and oxidative stress, which included superoxide reductase, rubredoxin, a Fe-Mn family superoxide dismutase, a KatG catalase-peroxidase, and a cytochrome bd ubiquinol oxidase. The low redox potential and intense anaerobic sulfate reduction provide evidence for the generally reduced conditions in the Belokurikha deep horizons. Spatial separation of aerobes and anaerobes in the water-rock system, similar to the one occurring in the terrestrial microbial mats, may be hypothesized, as well as occurring of aerobic processes in microniches. [ABSTRACT FROM AUTHOR] more...
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- 2023
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37. Coexistence of sulfate-reducing and methane-producing populations in upflow anaerobic sludge bed reactor treating lignocellulosic effluent with material balance.
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Yangin-Gomec, C. and Yarsur, E.
- Abstract
Microbial shift and predominance profile of a mesophilic upflow anaerobic sludge bed (UASB) reactor treating lignocellulose-rich wastewater [i.e., paper industry effluent with 665 mg SO
4</subscript> −2 /L and COD/SO4 = 2–8] was investigated using next-generation sequencing (NGS) technology in accordance with a mass balance determination on sulfur and organic compounds. Since paper producing industries generate wastewaters with high organic and sulfate concentrations, coexistence of microbial-especially of sulfur-reducing and methane-producing-communities and their interactions have been also searched out considering the impact on biogas yield (~ 0.16 L CH4 /g CODremoved ) and sulfate reduction (up to 82%). Analysis of the microbiomes by Illumina sequencing showed that Desulfovibrio spp. were the detected sulfate-reducing bacteria (SRB) coexisting with methane-producing archaea (MPA). Despite no evident inhibition of relatively high sulfate on biogas generation, predominance of Euryarchaeota decreased by nearly half and taxonomic classification revealed a shift of microbial population from aceticlastic (Methanosaeta) to hydrogenotrophic (Methanolinea) methanogens as operation continued probably due to their general tendency to dominate in stressed condition. Bacteroidetes, Firmicutes, and Proteobacteria (involving major SRB genera in the delta subclass) phyla had the highest ratios of relative abundances demonstrating the crucial role of their coexistence during the removal of the pollutants in lignocellulosic wastewaters. [ABSTRACT FROM AUTHOR] more...- Published
- 2023
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38. Desulfovibrio mangrovi sp. nov., a sulfate-reducing bacterium isolated from mangrove sediments: a member of the proposed genus "Psychrodesulfovibrio".
- Author
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Zhou, Jinjie, Zhang, Cui-Jing, and Li, Meng
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"Psychrodesulfovibrio", a proposed genus within the family Desulfovibrionaceae, is a group of sulfate-reducing bacteria with biogeochemical significance but restricted child taxa availability. In this study, a strictly anaerobic bacterium, designed strain FT415
T , was isolated from mangrove sediments in Futian Mangrove Nature Reserve in Shenzhen, China. The strain was Gram-stain-negative, motile, and vibrio-shaped with a single polar flagellum, which grew at the temperature range of 15–42 °C (optimum 37 °C), pH range of 6.0–7.5 (optimum 6.8), and in the presence of 0–36 g l−1 NaCl (optimum 6 g l−1 NaCl). In the presence of sulfate, electron donors including lactate, ethanol, pyruvate, malate, fumarate, succinate, cysteine, and glycerol were incompletely oxidized to acetate, and H2 and formate were used as electron donors with acetate as the carbon source by strain FT415T . Sulfate, thiosulfate, sulfide, and anthraquinone-2,6-disulfonate were reduced in the presence of lactate. Fe(III) oxide was reduced without cell growth. Fermentative growth was observed with pyruvate and cysteine. Vitamins were not required for growth. The major cellular fatty acids (> 10%) were C16:0 , summed feature 10 (C18:1 c11/t9/t6 and/or unknown ECL 17.834), C16:1 cis 9, and C18:0 . The major polar lipids were phosphatidylethanolamine, phospholipids, and aminolipids. The predominant menaquinone was MK-6(H2 ). The genomic DNA G+C content was 56.7%. Phylogenetic analysis showed that strain FT415T shared a 98.1% similarity in 16S rRNA gene sequence, an average nucleotide identity value of 84.0%, an average amino-acid identity value of 85.4%, and a digital DNA-DNA hybridization value of 25.7% with its closest relative Desulfovibrio subterraneus HN2T , which has been proposed to be transferred to the genus "Psychrodesulfovibrio". Based on phenotypic, phylogenetic, and genotypic evidence, a new species of the family Desulfovibrionaceae, Desulfovibrio mangrovi sp. nov. was proposed with the type strain FT415T (=GDMCC 1.3410T =KCTC 25525T ). [ABSTRACT FROM AUTHOR] more...- Published
- 2023
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39. The link between increased Desulfovibrio and disease severity in Parkinson's disease.
- Author
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Nie, Shiqing, Jing, Zhongwang, Wang, Jichen, Deng, Ye, Zhang, Yingshuang, Ye, Zheng, and Ge, Yuan
- Subjects
- *
PARKINSON'S disease , *HYDROGEN sulfide , *GUT microbiome , *SHOTGUN sequencing , *NEURODEGENERATION , *INCURABLE diseases - Abstract
Parkinson's disease (PD), a progressive and incurable neurodegenerative disease, has taken a huge economic toll and medical burden on our society. Increasing evidence has shown a strong link between PD and the gut microbiome, but studies on the relationship between the gut microbiome and the severity of PD are limited. In this study, 90 fecal samples were collected from newly diagnosed and untreated patients with PD (n = 47) and matched healthy control subjects (n = 43). The 16S rRNA amplicon and shotgun metagenomic sequencing was performed, aiming to uncover the connection between the gut microbiome and disease severity in PD. The results showed that Desulfovibrio was significantly increased in PD compared to healthy controls and positively correlated with disease severity. The increase in Desulfovibrio was mainly driven by enhanced homogeneous selection and weakened drift. Moreover, through metagenome-assembled genomes (MAGs) analysis, a Desulfovibrio MAG (MAG58) was obtained which was also positively correlated with disease severity. MAG58 possesses a complete assimilatory sulfate reduction pathway and a near-complete dissimilatory sulfate reduction pathway to produce hydrogen sulfide which may influence the development of PD. Based on these results, a potential pathogenic mechanism was presented to illustrate how the increased Desulfovibrio accelerates the development of PD by producing excessive hydrogen sulfide. The present study highlighted the vital role of Desulfovibrio in the development of PD, which may provide a new target for the diagnosis and treatment of PD. Key Points: • The evidence for the link between increased Desulfovibrio and disease severity in PD • A Desulfovibrio MAG was obtained which was correlated with PD • A model was presented to illustrate how increased Desulfovibrio causes PD [ABSTRACT FROM AUTHOR] more...
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- 2023
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40. T cell–mediated regulation of the microbiota protects against obesity
- Author
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Petersen, Charisse, Bell, Rickesha, Klag, Kendra A, Lee, Soh-Hyun, Soto, Raymond, Ghazaryan, Arevik, Buhrke, Kaitlin, Ekiz, H Atakan, Ost, Kyla S, Boudina, Sihem, O'Connell, Ryan M, Cox, James E, Villanueva, Claudio J, Stephens, W Zac, and Round, June L more...
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Microbiology ,Biological Sciences ,Biomedical and Clinical Sciences ,Prevention ,Obesity ,Nutrition ,Genetics ,2.1 Biological and endogenous factors ,Aetiology ,Metabolic and endocrine ,Animals ,Antibiosis ,Clostridium ,Desulfovibrio ,Host Microbial Interactions ,Intestinal Absorption ,Lipid Metabolism ,Metabolic Syndrome ,Mice ,Mice ,Mutant Strains ,Microbiota ,Myeloid Differentiation Factor 88 ,T-Lymphocytes ,Regulatory ,General Science & Technology - Abstract
The microbiota influences obesity, yet organisms that protect from disease remain unknown. During studies interrogating host-microbiota interactions, we observed the development of age-associated metabolic syndrome (MetS). Expansion of Desulfovibrio and loss of Clostridia were key features associated with obesity in this model and are present in humans with MetS. T cell-dependent events were required to prevent disease, and replacement of Clostridia rescued obesity. Inappropriate immunoglobulin A targeting of Clostridia and increased Desulfovibrio antagonized the colonization of beneficial Clostridia. Transcriptional and metabolic analysis revealed enhanced lipid absorption in the obese host. Colonization of germ-free mice with Clostridia, but not Desulfovibrio, down-regulated genes that control lipid absorption and reduced adiposity. Thus, immune control of the microbiota maintains beneficial microbial populations that constrain lipid metabolism to prevent MetS. more...
- Published
- 2019
41. Bulk phase resource ratio alters carbon steel corrosion rates and endogenously produced extracellular electron transfer mediators in a sulfate-reducing biofilm
- Author
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Krantz, Gregory P, Lucas, Kilean, Wunderlich, Erica L-, Hoang, Linh T, Avci, Recep, Siuzdak, Gary, and Fields, Matthew W
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Biological Sciences ,Ecology ,Biofilms ,Biofouling ,Biological Transport ,Corrosion ,Desulfovibrio ,Electrons ,Oxidation-Reduction ,Steel ,Sulfates ,Biocorrosion ,MIC ,microbiologically-influenced corrosion ,Environmental Sciences ,Technology ,Marine Biology & Hydrobiology ,Biological sciences ,Environmental sciences - Abstract
Desulfovibrio alaskensis G20 biofilms were cultivated on 316 steel, 1018 steel, or borosilicate glass under steady-state conditions in electron-acceptor limiting (EAL) and electron-donor limiting (EDL) conditions with lactate and sulfate in a defined medium. Increased corrosion was observed on 1018 steel under EDL conditions compared to 316 steel, and biofilms on 1018 carbon steel under the EDL condition had at least twofold higher corrosion rates compared to the EAL condition. Protecting the 1018 metal coupon from biofilm colonization significantly reduced corrosion, suggesting that the corrosion mechanism was enhanced through attachment between the material and the biofilm. Metabolomic mass spectrometry analyses demonstrated an increase in a flavin-like molecule under the 1018 EDL condition and sulfonates under the 1018 EAL condition. These data indicate the importance of S-cycling under the EAL condition, and that the EDL is associated with increased biocorrosion via indirect extracellular electron transfer mediated by endogenously produced flavin-like molecules. more...
- Published
- 2019
42. Genomic insight into iron acquisition by sulfate-reducing bacteria in microaerophilic environments.
- Author
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Barton, Larry L., Duarte, Americo G., and Staicu, Lucian C.
- Abstract
Historically, sulfate-reducing bacteria (SRB) have been considered to be strict anaerobes, but reports in the past couple of decades indicate that SRB tolerate exposure to O
2 and can even grow in aerophilic environments. With the transition from anaerobic to microaerophilic conditions, the uptake of Fe(III) from the environment by SRB would become important. In evaluating the metabolic capability for the uptake of iron, the genomes of 26 SRB, representing eight families, were examined. All SRB reviewed carry genes (feoA and feoB) for the ferrous uptake system to transport Fe(II) across the plasma membrane into the cytoplasm. In addition, all of the SRB genomes examined have putative genes for a canonical ABC transporter that may transport ferric siderophore or ferric chelated species from the environment. Gram-negative SRB have additional machinery to import ferric siderophores and ferric chelated species since they have the TonB system that can work alongside any of the outer membrane porins annotated in the genome. Included in this review is the discussion that SRB may use the putative siderophore uptake system to import metals other than iron. [ABSTRACT FROM AUTHOR] more...- Published
- 2023
- Full Text
- View/download PDF
43. Antibiotic-Resistant Desulfovibrio Produces H 2 S from Supplements for Animal Farming.
- Author
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Karnachuk, Olga V., Beletsky, Alexey V., Rakitin, Andrey L., Ikkert, Olga P., Avakyan, Marat R., Zyusman, Viacheslav S., Napilov, Andrei, Mardanov, Andrey V., and Ravin, Nikolai V.
- Subjects
HORIZONTAL gene transfer ,SWINE manure ,DOMESTIC animals ,ELECTROPHILES ,DRUG resistance in bacteria - Abstract
Sulphate-reducing bacteria, primarily Desulfovibrio, are responsible for the active generation of H
2 S in swine production waste. The model species for sulphate reduction studies, Desulfovibrio vulgaris strain L2, was previously isolated from swine manure characterized by high rates of dissimilatory sulphate reduction. The source of electron acceptors in low-sulphate swine waste for the high rate of H2 S formation remains uncertain. Here, we demonstrate the ability of the L2 strain to use common animal farming supplements including L-lysine-sulphate, gypsum and gypsum plasterboards as electron acceptors for H2 S production. Genome sequencing of strain L2 revealed the presence of two megaplasmids and predicted resistance to various antimicrobials and mercury, which was confirmed in physiological experiments. Most of antibiotic resistance genes (ARG) are carried by two class 1 integrons located on the chromosome and on the plasmid pDsulf-L2-2. These ARGs, predicted to confer resistance to beta-lactams, aminoglycosides, lincosamides, sulphonamides, chloramphenicol and tetracycline, were probably laterally acquired from various Gammaproteobacteria and Firmicutes. Resistance to mercury is likely enabled by two mer operons also located on the chromosome and on pDsulf-L2-2 and acquired via horizontal gene transfer. The second megaplasmid, pDsulf-L2-1, encoded nitrogenase, catalase and type III secretion system suggesting close contact of the strain with intestinal cells in the swine gut. The location of ARGs on mobile elements allows us to consider D. vulgaris strain L2 as a possible vector transferring antimicrobials resistance determinants between the gut microbiote and microbial communities in environmental biotopes. [ABSTRACT FROM AUTHOR] more...- Published
- 2023
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44. An essential role of the reversible electron-bifurcating hydrogenase Hnd for ethanol oxidation in Solidesulfovibrio fructosivorans.
- Author
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Kpebe, Arlette, Guendon, Chloé, Payne, Natalie, Ros, Julien, Berbar, Manel Khelil, Lebrun, Régine, Baffert, Carole, Shintu, Laetitia, and Brugna, Myriam
- Subjects
ETHANOL ,HYDROGENASE ,NAD (Coenzyme) ,ALDEHYDE dehydrogenase ,ALCOHOL dehydrogenase ,ELECTRON donors ,BACTERIAL physiology ,OXIDOREDUCTASES - Abstract
The tetrameric cytoplasmic FeFe hydrogenase Hnd from Solidesulfovibrio fructosivorans (formely Desulfovibrio fructosovorans) catalyses H2 oxidation and couples the exergonic reduction of NAD+ to the endergonic reduction of a ferredoxin by using a flavin-based electron-bifurcating mechanism. Regarding its implication in the bacterial physiology, we previously showed that Hnd, which is non-essential when bacteria grow fermentatively on pyruvate, is involved in ethanol metabolism. Under these conditions, it consumes H2 to produce reducing equivalents for ethanol production as a fermentative product. In this study, the approach implemented was to compare the two S. fructosivorans WT and the hndD deletion mutant strains when grown on ethanol as the sole carbon and energy source. Based on the determination of bacterial growth, metabolite consumption and production, gene expression followed by RT-q-PCR, and Hnd protein level followed by mass spectrometry, our results confirm the role of Hnd hydrogenase in the ethanol metabolism and furthermore uncover for the first time an essential function for a Desulfovibrio hydrogenase. Hnd is unequivocally required for S. fructosivorans growth on ethanol, and we propose that it produces H2 from NADH and reduced ferredoxin generated by an alcohol dehydrogenase and an aldehyde ferredoxin oxidoreductase catalyzing the conversion of ethanol into acetate. The produced H2 could then be recycled and used for sulfate reduction. Hnd is thus a reversible hydrogenase that operates in H2-consumption by an electron-bifurcating mechanism during pyruvate fermentation and in H2-production by an electron-confurcating mechanism when the bacterium uses ethanol as electron donor. [ABSTRACT FROM AUTHOR] more...
- Published
- 2023
- Full Text
- View/download PDF
45. Active Sulfate-Reducing Bacterial Community in the Camel Gut.
- Author
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Karnachuk, Olga V., Panova, Inna A., Panov, Vasilii L., Ikkert, Olga P., Kadnikov, Vitaly V., Rusanov, Igor I., Avakyan, Marat R., Glukhova, Lubov B., Lukina, Anastasia P., Rakitin, Anatolii V., Begmatov, Shahjahon, Beletsky, Alexey V., Pimenov, Nikolai V., and Ravin, Nikolai V. more...
- Subjects
BACTERIAL communities ,CAMELS ,RADIOACTIVE tracers ,IRON sulfides ,SULFATE-reducing bacteria ,SULFIDE minerals ,ELECTROPHILES - Abstract
The diversity and activity of sulfate-reducing bacteria (SRB) in the camel gut remains largely unexplored. An abundant SRB community has been previously revealed in the feces of Bactrian camels (Camelus bactrianus). This study aims to combine the 16S rRNA gene profiling, sulfate reduction rate (SRR) measurement with a radioactive tracer, and targeted cultivation to shed light on SRB activity in the camel gut. Fresh feces of 55 domestic Bactrian camels grazing freely on semi-arid mountain pastures in the Kosh-Agach district of the Russian Altai area were analyzed. Feces were sampled in early winter at an ambient temperature of −15 °C, which prevented possible contamination. SRR values measured with a radioactive tracer in feces were relatively high and ranged from 0.018 to 0.168 nmol S cm
−3 day−1 . The 16S rRNA gene profiles revealed the presence of Gram-negative Desulfovibrionaceae and spore-forming Desulfotomaculaceae. Targeted isolation allowed us to obtain four pure culture isolates belonging to Desulfovibrio and Desulforamulus. An active SRB community may affect the iron and copper availability in the camel intestine due to metal ions precipitation in the form of sparingly soluble sulfides. The copper-iron sulfide, chalcopyrite (CuFeS2 ), was detected by X-ray diffraction in 36 out of 55 analyzed camel feces. In semi-arid areas, gypsum, like other evaporite sulfates, can be used as a solid-phase electron acceptor for sulfate reduction in the camel gastrointestinal tract. [ABSTRACT FROM AUTHOR] more...- Published
- 2023
- Full Text
- View/download PDF
46. Diversity and Comparison of Intestinal Desulfovibrio in Patients with Liver Cirrhosis and Healthy People.
- Author
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Lu, Gexue, Zhang, Yu, Ren, Yilin, Shi, Jin-Song, Xu, Zheng-Hong, and Geng, Yan
- Subjects
CIRRHOSIS of the liver ,SULFATE-reducing bacteria ,HYDROGEN sulfide ,LIVER diseases ,INDUSTRIAL capacity - Abstract
Desulfovibrio belongs to Sulfate-reducing bacteria (SRB), which are widely present in anaerobic environments, including the human gut. Desulfovibrio has been associated with many human diseases, including chronic liver disease. However, the characteristics and difference of Desulfovibrio from fecal samples of healthy volunteers (HV) and patients with liver cirrhosis (LC) have not been fully elucidated. Here, we isolated Desulfovibrio from the feces of 6 HV and 9 LC, and 88 Desulfovibrio strains were obtained. In the feces of HV, 55% of isolated strains were D. desulfuricans, followed by D. intestinalis (15%), D. simplex (11%), D. piger (9%), D. legallii (4%), Cupidesulfovibrio oxamicus (4%) and D. fairfieldensis (2%). However, only D. desulfuricans (60%) and C. oxamicus (40%) were isolated from fecal samples of patients with LC. Our results suggest that there was a significant difference in the desulfurization ability and the H
2 S production ability of different Desulfovibrio. Desulfovibrio. Furthermore, we found that Desulfovibrio isolated from the patients with LC generally had a higher hydrogen sulfide production capacity, gastrointestinal tolerance, and levels of antibiotic resistance than the same species isolated from HV. Our findings suggested that Desulfovibrio may be associated with the occurrence and development of liver cirrhosis. [ABSTRACT FROM AUTHOR] more...- Published
- 2023
- Full Text
- View/download PDF
47. Gas-Mediated Intestinal Microbiome Regulation Prompts the Methanol Extract of Schizonepetae Spica to Relieve Colitis.
- Author
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Ye, Xuewei, Cen, Yingxin, Wu, Kefei, Xu, Langyu, Ni, Jiahui, Zheng, Wenxin, and Liu, Wei
- Abstract
Intestinal dysbiosis plays an important role in the pathogenesis of colitis (UC). Schizonepetae Herba can achieve anti-inflammatory effects as a medicine and food homologous vegetable. Luteolin, eriodictyol, fisetin, and kaempferol are the main anti-inflammatory active compounds obtained through mass spectrometry from the methanol extract of Schizonepetae Spica (JJSM). JJSM intervention resulted in attenuated weight loss, high disease-activity-index score, colon length shortening and colonic pathological damage in DSS-induced colitis mice. Interestingly, hydrogen sulfide (H
2 S) was inhibited remarkably, which is helpful to elucidate the relationship between active substance and intestinal flora. Furthermore, JJSM administration improved intestinal flora with down-regulating the abundance of harmful bacteria such as Clostridiales and Desulfovibrio and up-regulating the abundance of beneficial bacteria such as Muribaculaceae and Ligolactobacillus and enhanced the production of SCFAs. It is worth noticing that Desulfovibrio is related to the production of intestinal gas H2 S. The elevated levels of Desulfovibrio and H2 S will hasten the onset of colitis, which is a crucial risk factor for colitis. The results displayed that JJSM could considerably ameliorate colitis by rebuilding H2 S-related intestinal flora, which provides a new therapeutic strategy for Schizonepetae Spica to be utilized as a functional food and considered as an emerging candidate for intestinal inflammation. [ABSTRACT FROM AUTHOR] more...- Published
- 2023
- Full Text
- View/download PDF
48. Desulfovibrio vulgaris as a model microbe for the study of corrosion under sulfate‐reducing conditions
- Author
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Toshiyuki Ueki and Derek R. Lovley
- Subjects
corrosion ,Desulfovibrio ,electron shuttle ,extracellular electron transfer ,Fe0 oxidation ,hydrogenase ,Microbiology ,QR1-502 - Abstract
Abstract Corrosion of iron‐containing metals under sulfate‐reducing conditions is an economically important problem. Microbial strains now known as Desulfovibrio vulgaris served as the model microbes in many of the foundational studies that developed existing models for the corrosion of iron‐containing metals under sulfate‐reducing conditions. Proposed mechanisms for corrosion by D. vulgaris include: (1) H2 consumption to accelerate the oxidation of Fe0 coupled to the reduction of protons to H2; (2) production of sulfide that combines with ferrous iron to form iron sulfide coatings that promote H2 production; (3) moribund cells release hydrogenases that catalyze Fe0 oxidation with the production of H2; (4) direct electron transfer from Fe0 to cells; and (5) flavins serving as an electron shuttle for electron transfer between Fe0 and cells. The demonstrated possibility of conducting transcriptomic and proteomic analysis of cells growing on metal surfaces suggests that similar studies on D. vulgaris corrosion biofilms can aid in identifying proteins that play an important role in corrosion. Tools for making targeted gene deletions in D. vulgaris are available for functional genetic studies. These approaches, coupled with instrumentation for the detection of low concentrations of H2, and proven techniques for evaluating putative electron shuttle function, are expected to make it possible to determine which of the proposed mechanisms for D. vulgaris corrosion are most important. more...
- Published
- 2022
- Full Text
- View/download PDF
49. An essential role of the reversible electron-bifurcating hydrogenase Hnd for ethanol oxidation in Solidesulfovibrio fructosivorans
- Author
-
Arlette Kpebe, Chloé Guendon, Natalie Payne, Julien Ros, Manel Khelil Berbar, Régine Lebrun, Carole Baffert, Laetitia Shintu, and Myriam Brugna
- Subjects
hydrogenase ,Hnd ,Desulfovibrio ,Solidesulfovibrio ,ethanol ,alcohol dehydrogenase ,Microbiology ,QR1-502 - Abstract
The tetrameric cytoplasmic FeFe hydrogenase Hnd from Solidesulfovibrio fructosivorans (formely Desulfovibrio fructosovorans) catalyses H2 oxidation and couples the exergonic reduction of NAD+ to the endergonic reduction of a ferredoxin by using a flavin-based electron-bifurcating mechanism. Regarding its implication in the bacterial physiology, we previously showed that Hnd, which is non-essential when bacteria grow fermentatively on pyruvate, is involved in ethanol metabolism. Under these conditions, it consumes H2 to produce reducing equivalents for ethanol production as a fermentative product. In this study, the approach implemented was to compare the two S. fructosivorans WT and the hndD deletion mutant strains when grown on ethanol as the sole carbon and energy source. Based on the determination of bacterial growth, metabolite consumption and production, gene expression followed by RT-q-PCR, and Hnd protein level followed by mass spectrometry, our results confirm the role of Hnd hydrogenase in the ethanol metabolism and furthermore uncover for the first time an essential function for a Desulfovibrio hydrogenase. Hnd is unequivocally required for S. fructosivorans growth on ethanol, and we propose that it produces H2 from NADH and reduced ferredoxin generated by an alcohol dehydrogenase and an aldehyde ferredoxin oxidoreductase catalyzing the conversion of ethanol into acetate. The produced H2 could then be recycled and used for sulfate reduction. Hnd is thus a reversible hydrogenase that operates in H2-consumption by an electron-bifurcating mechanism during pyruvate fermentation and in H2-production by an electron-confurcating mechanism when the bacterium uses ethanol as electron donor. more...
- Published
- 2023
- Full Text
- View/download PDF
50. Genome Editing Method for the Anaerobic Magnetotactic Bacterium Desulfovibrio magneticus RS-1
- Author
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Grant, Carly R, Rahn-Lee, Lilah, LeGault, Kristen N, and Komeili, Arash
- Subjects
Microbiology ,Biological Sciences ,Genetics ,Biotechnology ,Anaerobiosis ,Desulfovibrio ,Gene Editing ,Genome ,Bacterial ,Magnetosomes ,Mutagenesis ,Plasmids ,Reverse Genetics ,biomineralization ,genome editing ,iron ,magnetosomes ,magnetotactic bacteria ,organelles ,Medical microbiology - Abstract
Magnetosomes are complex bacterial organelles that serve as model systems for studying bacterial cell biology, biomineralization, and global iron cycling. Magnetosome biogenesis is primarily studied in two closely related Alphaproteobacteria of the genus Magnetospirillum that form cubooctahedral-shaped magnetite crystals within a lipid membrane. However, chemically and structurally distinct magnetic particles have been found in physiologically and phylogenetically diverse bacteria. Due to a lack of molecular genetic tools, the mechanistic diversity of magnetosome formation remains poorly understood. Desulfovibrio magneticus RS-1 is an anaerobic sulfate-reducing deltaproteobacterium that forms bullet-shaped magnetite crystals. A recent forward genetic screen identified 10 genes in the conserved magnetosome gene island of D. magneticus that are essential for its magnetic phenotype. However, this screen likely missed mutants with defects in crystal size, shape, and arrangement. Reverse genetics to target the remaining putative magnetosome genes using standard genetic methods of suicide vector integration have not been feasible due to the low transconjugation efficiency. Here, we present a reverse genetic method for targeted mutagenesis in D. magneticus using a replicative plasmid. To test this method, we generated a mutant resistant to 5-fluorouracil by making a markerless deletion of the upp gene that encodes uracil phosphoribosyltransferase. We also used this method for targeted marker exchange mutagenesis by replacing kupM, a gene identified in our previous screen as a magnetosome formation factor, with a streptomycin resistance cassette. Overall, our results show that targeted mutagenesis using a replicative plasmid is effective in D. magneticus and may also be applied to other genetically recalcitrant bacteria.IMPORTANCE Magnetotactic bacteria (MTB) are a group of organisms that form intracellular nanometer-scale magnetic crystals though a complex process involving lipid and protein scaffolds. These magnetic crystals and their lipid membranes, termed magnetosomes, are model systems for studying bacterial cell biology and biomineralization and are potential platforms for biotechnological applications. Due to a lack of genetic tools and unculturable representatives, the mechanisms of magnetosome formation in phylogenetically deeply branching MTB remain unknown. These MTB contain elongated bullet-/tooth-shaped magnetite and greigite crystals that likely form in a manner distinct from that of the cubooctahedral-shaped magnetite crystals of the genetically tractable MTB within the Alphaproteobacteria Here, we present a method for genome editing in Desulfovibrio magneticus RS-1, a cultured representative of the deeply branching MTB of the class Deltaproteobacteria This marks a crucial step in developing D. magneticus as a model for studying diverse mechanisms of magnetic particle formation by MTB. more...
- Published
- 2018
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