Search

Abstract Objective To summarize the reliability and validity of ultrasonography in evaluating the stiffness, excursion, stiffness, or strain rate of diaphragm, intercostals and abdominal muscles in healthy or non-hospitalized individuals. Literature search PubMed, Embase, SPORTDiscus, CINAHL and Cochrane Library were searched from inception to May 30, 2022. Study selection criteria Case–control, cross-sectional, and longitudinal studies were included if they investigated the reliability or validity of various ultrasonography technologies (e.g., brightness-mode, motion-mode, shear wave elastography) in measuring the thickness, excursion, stiffness, or strain rate of any respiratory muscles. Data synthesis Relevant data were summarized based on healthy and different patient populations. The methodological quality by different checklist depending on study design. The quality of evidence of each psychometric property was graded by the Grading of Recommendations, Assessment, Development and Evaluations, respectively. Results This review included 24 studies with 787 healthy or non-hospitalized individuals (e.g., lower back pain (LBP), adolescent idiopathic scoliosis (AIS), and chronic obstructive pulmonary disease (COPD)). Both inspiratory (diaphragm and intercostal muscles) and expiratory muscles (abdominal muscles) were investigated. Moderate-quality evidence supported sufficient (intra-class correlation coefficient > 0.7) within-day intra-rater reliability of B-mode ultrasonography in measuring right diaphragmatic thickness among people with LBP, sufficient between-day intra-rater reliability of M-mode ultrasonography in measuring right diaphragmatic excursion in non-hospitalized individuals. The quality of evidence for all other measurement properties in various populations was low or very low. High-quality evidence supported sufficient positive correlations between diaphragm excursion and forced expiratory volume in the first second or forced vital capacity (r > = 0.3) in healthy individuals. Conclusions Despite the reported sufficient reliability and validity of using ultrasonography to assess the thickness, excursion, stiffness, and strain rate of respiratory muscles in non-hospitalized individuals, further large-scale studies are warranted to improve the quality of evidence regarding using ultrasonography for these measurements in clinical practice. Researchers should establish their own reliability before using various types of ultrasonography to evaluate respiratory muscle functions. Trial registration PROSPERO NO. CRD42022322945.

TMD devices are considered a reasonable solution to enhance the bearing capacity of the structures under unpredictable impacts from external forces because their operating principle is quite simple and does not vary the main structure. This paper investigated the influence of the TMD device, including its mass and stiffness, on the vibration reduction of the primary structure, a basic steel beam. In which, a small-scale steel beam model with boundary conditions through experiments was carried out. Then, simulated the experiment using MATLAB based on the experimental results to study the effect of TMD on the vibration of the structure. The results exhibit that the vibration-damping capacity of the main structure increases as the TMD mass increases. The stiffness of the main structure is less than 1,8k for both TMD 3% and 5%, resulting in the rapid increase of oscillation reduction efficiency.

High-toughness resin concrete steel mesh (HTRCS) composites, as a novel reinforcement material, are extensively employed, yet there has been a lack of comprehensive quantitative studies on them, and our knowledge about them predominantly relies on experimental investigations. To delve into the shear performance of reinforced concrete beams fortified with HTRCS, this research executed four-point bending static load experiments on a benchmark of two standard beams and six HTRCS-reinforced beams. The results demonstrate that the shear bearing capacity of the reinforced concrete beams was notably enhanced with HTRCS, ranging from approximately 10% to 65%. Further examination revealed that the stiffness of the specimens is significantly influenced by the HTRCS thickness, shear–span ratio, and concrete strength, with the shear–span ratio exerting the most notable impact on stiffness. This analysis furnishes a solid theoretical foundation for the utilization of HTRCS.

Mechanical signals from the extracellular matrix are crucial in guiding cellular behavior. Two-dimensional hydrogel substrates for cell cultures serve as exceptional tools for mechanobiology studies because they mimic the biomechanical and adhesive characteristics of natural environments. However, the interdisciplinary knowledge required to synthetize and manipulate these biomaterials typically restricts their widespread use in biological laboratories, which may not have the material science expertise or specialized instrumentation. To address this, we propose a scalable method that requires minimal setup to produce 2D hydrogel substrates with independent modulation of the rigidity and adhesiveness within the range typical of natural tissues. In this method, norbornene-terminated 8-arm polyethylene glycol is stoichiometrically functionalized with RGD peptides and crosslinked with a di-cysteine terminated peptide via a thiol–ene click reaction. Since the synthesis process significantly influences the final properties of the hydrogels, we provide a detailed description of the chemical procedure to ensure reproducibility and high throughput results. We demonstrate examples of cell mechanosignaling by monitoring the activation state of the mechanoeffector proteins YAP/TAZ. This method effectively dissects the influence of biophysical and adhesive cues on cell behavior. We believe that our procedure will be easily adopted by other cell biology laboratories, improving its accessibility and practical application.

Although bolted joints may appear simple and are easy to manipulate, they are challenging to model and analyze due to their complex structural patterns and statically indeterminate nature. Ensuring the structural integrity of these joints requires maintaining proper bolt preload and clamping force, which is crucial for preventing failures such as overload, excessive bearing stress, fatigue, and stripping caused by seizing or galling. Achieving the necessary clamping force involves carefully controlling the input tightening torque, which is divided into the pitch torque and the friction torques at the bolt or nut bearing surfaces and in the engaged threads. The resulting clamping force is critical for generating the required force within the bolt. However, the achieved bolt force depends on several factors, such as friction at the joint’s contact surfaces, grip length, and the relative rotation between the bolt and nut during tightening. Friction at the contact surfaces, particularly beneath the bolt head or nut and between the threads, consumes a significant portion of the applied tightening torque—approximately 90%. This paper explores the three existing bolt internal pitch, bearing, and thread friction torques that are generated by the external applied torque in a bolted joint, as well as their contributions and variations throughout a loading cycle composed of three phases: tightening, settling, and untightening. An analytical model is developed to determine these torque components, and its results are compared with those obtained from finite element (FE) modeling and experimental testing from previous studies. Finally, this study examines the torque–tension relationship during bolt tightening, offering insights into the required accuracy of bolt and clamped member stiffness. The bolt samples used in this study include M12 × 1.75 and M36 × 4 hex bolts.

The dimensions of tenons in solid wood furniture significantly influence the mechanical performance of mortise and tenon joints. While previous studies have primarily focused on tenon length, width, and thickness, they often overlooked the impact of clearance between the mortise and tenon. This study investigates the effects of tenon length, tenon width, and clearance on the mechanical performance of mortise and tenon joints, aiming to enhance their bending moment capacity (BMC) and stiffness. A three-factor, three-level orthogonal test was conducted, utilizing range analysis and variance analysis to assess the effects of each factor on BMC and stiffness. The LSD post hoc test was employed to identify significant differences between levels of the same factor, and nonlinear regression analysis was used to fit the experimental results. Based on orthogonal experiment outcomes, a grey relational theory-based evaluation system was developed to assess the comprehensive performance of joints, including both moment capacity and stiffness. Results indicate that tenon length has the most significant effect on BMC, followed by clearance and tenon width, while clearance has the greatest impact on stiffness, followed by tenon length and tenon width. These findings are consistent with those obtained from grey relational analysis. When considering both BMC and stiffness as a comprehensive evaluation, the optimal combination is a tenon length of 40 mm, a tenon width of 35 mm, and a clearance of −0.1 mm. This study offers valuable insights for the rational design of mortise and tenon joints, contributing to improved performance and reduced manufacturing costs.

Purpose: Glaucoma, one of the leading causes of irreversible blindness, is a common progressive optic neuropathy characterised by visual field defects and structural changes to the optic nerve head (ONH). There is extracellular matrix (ECM) accumulation and fibrosis of the lamina cribrosa (LC) in the ONH, and consequently increased tissue stiffness of the LC connective tissue. Integrins are cell surface proteins that provide the key molecular link connecting cells to the ECM and serve as bidirectional sensors transmitting signals between cells and their environment to promote cell adhesion, proliferation, and remodelling of the ECM. Here, we investigated the expression of αVβ3 integrin in glaucoma LC cell, and its effect on stiffness-induced ECM gene transcription and cellular proliferation rate in normal (NLC) and glaucoma (GLC) LC cells, by down-regulating αVβ3 integrin expression using cilengitide (a known potent αVβ3 and αVβ5 inhibitor) and β3 integrin siRNA knockdown. Methods: GLC cells were compared to age-matched controls NLC to determine differential expression levels of αVβ3 integrin, ECM genes (Col1A1, α-SMA, fibronectin, vitronectin), and proliferation rates. The effects of αVβ3 integrin blockade (with cilengitide) and silencing (with a pool of four predesigned αVβ3 integrin siRNAs) on ECM gene expression and proliferation rates were evaluated using both reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting in the human NLC cells cultured on soft (4 kPa) and stiff (100 kPa) substrate and in GLC cells grown on standard plastic plates. Results: αVβ3 integrin gene and protein expression were enhanced (p < 0.05) in GLC cells as compared to NLC. Both cilengitide and siRNA significantly reduced αVβ3 expression in GLC. When NLC were grown in the stiff substrate, cilengitide and siRNA also significantly reduced the increased expression in αVβ3, ECM components, and proliferation rate. Conclusions: Here, we provide evidence of cilengitide- and siRNA-mediated silencing of αVβ3 integrin expression, and inhibition of ECM synthesis in LC cells. Therefore, αVβ3 integrin may be a promising target for the development of novel anti-fibrotic therapies for treating the LC cupping of the ONH in glaucoma.

BackgroundMagnetic resonance (MR) elastography of the liver measures hepatic stiffness, which correlates with the histopathological staging of liver fibrosis. Conventional Cartesian gradient-echo (GRE) MR elastography requires breath-holding, which is challenging for children. Non-Cartesian radial free-breathing MR elastography is a potential solution to this problem.ObjectiveTo investigate radial free-breathing MR elastography for measuring hepatic stiffness in children.Materials and methodsIn this prospective pilot study, 14 healthy children and 9 children with liver disease were scanned at 3 T using 2-D Cartesian GRE breath-hold MR elastography (22 s/slice) and 2-D radial GRE free-breathing MR elastography (163 s/slice). Each sequence was acquired twice. Agreement in the stiffness measurements was evaluated using Lin's concordance correlation coefficient (CCC) and within-subject mean difference. The repeatability was assessed using the within-subject coefficient of variation and intraclass correlation coefficient (ICC).ResultsFourteen healthy children and seven children with liver disease completed the study. Median (±interquartile range) normalized measurable liver areas were 62.6% (±26.4%) and 44.1% (±39.6%) for scan 1, and 60.3% (±21.8%) and 43.9% (±44.2%) for scan 2, for Cartesian and radial techniques, respectively. Hepatic stiffness from the Cartesian and radial techniques had close agreement with CCC of 0.89 and 0.94, and mean difference of 0.03 kPa and -0.01 kPa, for scans 1 and 2. Cartesian and radial techniques achieved similar repeatability with within-subject coefficient of variation=1.9% and 3.4%, and ICC=0.93 and 0.92, respectively.ConclusionIn this pilot study, radial free-breathing MR elastography was repeatable and in agreement with Cartesian breath-hold MR elastography in children.

The stiffness (K) and slenderness factor (λ) of a steel plate-based damper has been studied on the basis of elastic-inelastic-plastic buckling (EIP) modes and flexural/shear/flexural-shear failure mechanisms (FSF-S), which has been designed for the improvement of the behavior of concentrically braced frames. Steel plate-based dampers offer significant benefits in terms of mode shapes and failure mechanisms, contributing to improved dynamic performance, enhanced structural resilience, and increased safety of civil engineering structures. Their effectiveness in mitigating dynamic loads makes them a valuable tool for engineers designing structures to withstand extreme environmental conditions and seismic events. This study was undertaken by using the learning abilities of the response surface methodology (RSM), artificial neural network (ANN) and the evolutionary polynomial regression (EPR). Steel plate dampers are special structural designs used to withstand the effect of special loading conditions especially seismic effects. Its design based on the prediction of its stiffness (K) and slenderness factor (λ) cannot be overlooked in the present-day artificial intelligence technology. In this research work, thirty-three entries based on the steel plate damper geometrical properties were recorded and deployed for the intelligent forecast of the fundamental properties (λ and K). Design ratios of the steel plate damper properties were considered and models behavior was recorded. From the outcome of the model, it can be observed that even though the EPR and ANN in that order outclassed the other techniques, the RSM produced model minimization and maximization features of the desirability levels, color factor scales and 3D surface observation, which shows the real model behaviors. Overall, the EPR with R2 of 0.999 and 1.000 for the λ and K, respectively showed to be the decisive model but the RSM has features that can be beneficial to the structural design of the studied steel plate damper for a more robust and sustainable construction. With these performances recorded in this exercise, the techniques have shown their potential to be applied in the prediction of steel damper stiffness with optimized characteristic features to withstand structural stresses. [ABSTRACT FROM AUTHOR]

Purpose: Although several studies investigated the effect of plyometric training on physical performance, there is a lack of clarity regarding the effectiveness of plyometric training or its moderator variables in youth female volleyball players. The primary aim of this study was to explore the effect of horizontal plyometric training on explosive stretch-shortening cycle hops and jumps in the vertical and horizontal directions in female post peak height velocity (PHV) volleyball players. The secondary aim was to assess the influence of participant and training related moderators on horizontal plyometric training in post-PHV volleyball players. Methods: A total of 23 post-PHV volleyball players participated in this 8-week intervention with horizontal plyometric exercises, twice a week. Pre-testing and post-testing included bilateral and unilateral vertical sub-maximal hopping, horizontal jumping and hopping, and a drop jump test. The effectiveness of the intervention was assessed using a paired t-test. The influence of internal moderators such as age, maturity and body mass and external moderators such as training volume were assessed using regression and correlation analysis. Results: An 8-week plyometric training improved sub-maximal hopping at 2.5 Hz left by 4.4%, bilateral sub-maximal hopping at 2.0 Hz by 9.5% and bilateral submaximal hopping at 2.2 Hz by 6.8% in post-PHV female volleyball players. Horizontal jumping and hopping, reactive strength index and other submaximal hopping conditions did not improve significantly. Body mass had a large moderating effect on vertical unilateral sub-maximal hopping at 2.5 Hz right (p = 0.010, η² = 0.314), vertical unilateral hopping at 3.0 Hz right (p = 0.035, η² = 0.170), and vertical unilateral hopping at 3.0 Hz left (p = 0.043, η² = 0.203). Training volume together with generalized joint hypermobility moderated right leg triple broad hop performance, whereas maturity and age did not moderate any variables. Conclusion: This study determined that 8 weeks of horizontal plyometric training can improve unilateral absolute leg stiffness in post-PHV female volleyball players, and this training effect can be moderated by body mass. Furthermore, the training effect on triple hopping performance on the right leg can be moderated by combined training volume with generalized joint hypermobility. [ABSTRACT FROM AUTHOR]

The intricate nature of native cartilage, characterized by zonal variations in oxygen levels and ECM composition, poses a challenge for existing hydrogelbased tissue models. Consequently, these 3D models often present simplified renditions of the native tissue, failing to fully capture its heterogenous nature. The combined effects of hydrogel components, network properties, and structural designs on cellular responses are often overlooked. In this work, we aim to establish more physiological cartilage models through biofabrication of photopolymerizable allylated-gelatin (GelAGE) and Thiolated Heparin (HepSH) constructs with tailorable matrix stiffness and customized architectures. This involves systematically studying how the native glycosaminoglycan Heparin together with hydrogel stiffness, and oxygen availability within 3D structures influence chondrogenic differentiation and regional heterogeneity. A comprehensive library of 3D hydrogel constructs was successfully developed, encompassing GelAGE-HepSH hydrogels with three distinct stiffness levels: 12, 55 and 121 kPa, and three unique geometries: spheres, discs, and square lattices. In soft GelAGE-HepSH hydrogels, the localization of differentiating cells was observed to be irregular, while stiff hydrogels restricted the overall secretion of ECM components. The medium-stiff hydrogels were found to be most applicable, supporting both uniform tissue formation and maintained shape fidelity. Three different 3D architectures were explored, where biofabrication of smaller GelAGE-HepSH spheres without oxygen gradients induced homogenous, hyaline cartilage tissue formation. Conversely, fabrication of larger constructs (discs and lattices) with oxygen gradients could be utilized to design heterogenous cartilage tissue models. Similarly, temporal oxygen gradients were observed to drive interconnected deposition of glycosaminoglycans (GAGs). Control samples of GelAGE without HepSH did not exhibit any notable changes in chondrogenesis as a function of stiffness, architectures, or oxygen concentrations. Overall, the incorporation of HepSH within GelAGE hydrogels was observed to serve as an amplifier for the biological effects from both stiffness and oxygen cues. In conclusion, fabrication of GelAGE-HepSH constructs designed to impose limitations on oxygen availability induce more zone-specific cartilage tissue alignment. This systematic study of matrix components, network stiffness, and oxygen levels in 3D biofabricated structures contributes to the development of more physiologically relevant cartilage models while further enhancing our overall understanding of cartilage tissue engineering. [ABSTRACT FROM AUTHOR]

Simple Summary: We aimed to investigate the intrinsic biomechanical properties of melanoma cancer cells. We demonstrate that the modulation of stiffness properties, and their correlated morphometrics, impacts cutaneous melanoma cell invasiveness following EMT switching and Tspan8 transcriptional activation. Thus, we propose that melanoma stiffness measurement could be of prognostic relevance for patients. The intrinsic biomechanical properties of cancer cells remain poorly understood. To decipher whether cell stiffness modulation could increase melanoma cells' invasive capacity, we performed both in vitro and in vivo experiments exploring cell stiffness by atomic force microscopy (AFM). We correlated stiffness properties with cell morphology adaptation and the molecular mechanisms underlying epithelial-to-mesenchymal (EMT)-like phenotype switching. We found that melanoma cell stiffness reduction was systematically associated with the acquisition of invasive properties in cutaneous melanoma cell lines, human skin reconstructs, and Medaka fish developing spontaneous MAP-kinase-induced melanomas. We observed a systematic correlation of stiffness modulation with cell morphological changes towards mesenchymal characteristic gains. We accordingly found that inducing melanoma EMT switching by overexpressing the ZEB1 transcription factor, a major regulator of melanoma cell plasticity, was sufficient to decrease cell stiffness and transcriptionally induce tetraspanin-8-mediated dermal invasion. Moreover, ZEB1 expression correlated with Tspan8 expression in patient melanoma lesions. Our data suggest that intrinsic cell stiffness could be a highly relevant marker for human cutaneous melanoma development. [ABSTRACT FROM AUTHOR]

The delivery of nanomedicines into cells holds enormous therapeutic potential; however little is known regarding how the extracellular matrix (ECM) can influence cell–nanoparticle (NP) interactions. Changes in ECM organization and composition occur in several pathophysiological states, including fibrosis and tumorigenesis, and may contribute to disease progression. We show that the physical characteristics of cellular substrates, that more closely resemble the ECM in vivo, can influence cell behavior and the subsequent uptake of NPs. Electrospinning was used to create two different substrates made of soft polyurethane (PU) with aligned and non-aligned nanofibers to recapitulate the ECM in two different states. To investigate the impact of cell–substrate interaction, A549 lung epithelial cells and MRC-5 lung fibroblasts were cultured on soft PU membranes with different alignments and compared against stiff tissue culture plastic (TCP)/glass. Both cell types could attach and grow on both PU membranes with no signs of cytotoxicity but with increased cytokine release compared with cells on the TCP. The uptake of silica NPs increased more than three-fold in fibroblasts but not in epithelial cells cultured on both membranes. This study demonstrates that cell–matrix interaction is substrate and cell-type dependent and highlights the importance of considering the ECM and tissue mechanical properties when designing NPs for effective cell targeting and treatment. [ABSTRACT FROM AUTHOR]

Physical forces are ubiquitous in biological processes across scales and diverse contexts. This review highlights the significance of mechanical forces in nervous system development, homeostasis, and disease. We provide an overview of mechanical signals present in the nervous system and delve into mechanotransduction mechanisms translating these mechanical cues into biochemical signals. During development, mechanical cues regulate a plethora of processes, including cell proliferation, differentiation, migration, network formation, and cortex folding. Forces then continue exerting their influence on physiological processes, such as neuronal activity, glial cell function, and the interplay between these different cell types. Notably, changes in tissue mechanics manifest in neurodegenerative diseases and brain tumors, potentially offering new diagnostic and therapeutic target opportunities. Understanding the role of cellular forces and tissue mechanics in nervous system physiology and pathology adds a new facet to neurobiology, shedding new light on many processes that remain incompletely understood. Pillai and Franze provide a comprehensive overview of recent advancements in our understanding of how mechanical cues, e.g., tissue stiffness and cellular forces, contribute to nervous system development, homeostasis, and disorder. They illuminate experimental approaches, mechanotransduction pathways, and open questions. [ABSTRACT FROM AUTHOR]

The extracellular matrix (ECM) is a significant constituent of tumors, fulfilling various essential functions such as providing mechanical support, influencing the microenvironment, and serving as a reservoir for signaling molecules. The abundance and degree of cross-linking of ECM components are critical determinants of tissue stiffness. In the process of tumorigenesis, the interaction between ECM and immune cells within the tumor microenvironment (TME) frequently leads to ECM stiffness, thereby disrupting normal mechanotransduction and promoting malignant progression. Therefore, acquiring a thorough comprehension of the dysregulation of ECM within the TME would significantly aid in the identification of potential therapeutic targets for cancer treatment. In this regard, we have compiled a comprehensive summary encompassing the following aspects: (1) the principal components of ECM and their roles inmalignant conditions; (2) the intricate interaction between ECM and immune cells within the TME; and (3) the pivotal regulators governing the onco-immune response in ECM. [ABSTRACT FROM AUTHOR]

Tumor diseases become a huge problem when they embark on a path that advances to malignancy, such as the process of metastasis. Cancer metastasis has been thoroughly investigated from a biological perspective in the past, whereas it has still been less explored from a physical perspective. Until now, the intraluminal pathway of cancer metastasis has received the most attention, while the interaction of cancer cells with macrophages has received little attention. Apart from the biochemical characteristics, tumor treatments also rely on the tumor microenvironment, which is recognized to be immunosuppressive and, as has recently been found, mechanically stimulates cancer cells and thus alters their functions. The review article highlights the interaction of cancer cells with other cells in the vascular metastatic route and discusses the impact of this intercellular interplay on the mechanical characteristics and subsequently on the functionality of cancer cells. For instance, macrophages can guide cancer cells on their intravascular route of cancer metastasis, whereby they can help to circumvent the adverse conditions within blood or lymphatic vessels. Macrophages induce microchannel tunneling that can possibly avoid mechanical forces during extra- and intravasation and reduce the forces within the vascular lumen due to vascular flow. The review article highlights the vascular route of cancer metastasis and discusses the key players in this traditional route. Moreover, the effects of flows during the process of metastasis are presented, and the effects of the microenvironment, such as mechanical influences, are characterized. Finally, the increased knowledge of cancer metastasis opens up new perspectives for cancer treatment. [ABSTRACT FROM AUTHOR]

Flying cable-driven parallel robots (CDPRs) are a special subclass of cable-driven robots that offer mobility in air compared with the traditional CDPRs. These flying CDPRs possess weaker stiffness due to their high maneuverability and cable's unilaterality, which may result in fluctuations around a desired nominal moving platform pose. The focus of this study is on the derivation of the overall stiffness and enhancement method of stiffness with regard to the weakest degree of freedom. The overall stiffness is divided into two parts, namely, active and passive stiffnesses. The line geometry theory is introduced to derive the explicit expression of the active stiffness, which is a 3D Hessian matrix. Results showed that the rotational stiffness around the z-axis kzz is the weakest stiffness based on the overall stiffness matrix expression. Furthermore, we summarize the stiffness distribution of the flying CDPR in the entire workspace. Specifically, we present a stiffness-oriented cable tension distribution algorithm to achieve the best feasible stiffness considering the enhancement of kzz and tensions' limit, which is only applicable for the flying CDPR with redundant actuation. Simulation results demonstrate that the proposed algorithm can remarkably enhance stiffness. [ABSTRACT FROM AUTHOR]

Background: This study aimed to: (1) identify assessment methods that can detect greater ankle dorsiflexion range of motion (DROM) limitation in the injured limb; (2) determine whether differences in weightbearing measurements exist even in the absence of DROM limitations in the injured limb according to non-weightbearing measurements; and (3) examine associations between DROM in the weightbearing and non-weightbearing positions and compare those between a patient group with foot and ankle injuries and a healthy group. Methods: Eighty-two patients with foot and ankle injuries (e.g., fractures, ligament and tendon injuries) and 49 healthy individuals participated in this study. Non-weightbearing DROM was measured under two different conditions: prone position with knee extended and prone position with knee flexed. Weightbearing DROM was measured as the tibia inclination angle (weightbearing angle) and distance between the big toe and wall (weightbearing distance) at maximum dorsiflexion. The effects of side (injured, uninjured) and measurement method on DROM in the patient groups were assessed using two-way repeated-measures ANOVA and t-tests. Pearson correlations between measurements were assessed. In addition, we analyzed whether patients without non-weightbearing DROM limitation (≤ 3 degrees) showed limitations in weightbearing DROM using t-tests with Bonferroni correction. Results: DROM in patient groups differed significantly between legs with all measurement methods (all: P < 0.001), with the largest effect size for weightbearing angle (d = 0.95). Patients without non-weightbearing DROM limitation (n = 37) displayed significantly smaller weightbearing angle and weightbearing distance on the injured side than on the uninjured side (P < 0.001 each), with large effect sizes (d = 0.97–1.06). Correlation coefficients between DROM in non-weightbearing and weightbearing positions were very weak (R = 0.17, P = 0.123) to moderate (R = 0.26–0.49, P < 0.05) for the patient group, and moderate to strong for the healthy group (R = 0.51–0.69, P < 0.05). Conclusions: DROM limitations due to foot and ankle injuries may be overlooked if measurements are only taken in the non-weightbearing position and should also be measured in the weightbearing position. Furthermore, DROM measurements in non-weightbearing and weightbearing positions may assess different characteristics, particularly in patient group. Level of evidence: Level IV, cross-sectional study. [ABSTRACT FROM AUTHOR]

The potential of faults to show earthquake‐generating slip instabilities depends not only on the intrinsic frictional properties of the fault zone, but also on the elasticity of the surrounding material. A velocity‐weakening fault is expected to show increasingly unstable frictional behavior with decreasing local elastic stiffness around the fault zone. Fault zone roughness can cause slip in the shear direction to be accompanied by fault‐normal movement, modulated by fault‐normal elastic properties, however these effects are poorly understood. Here, we systematically vary the stiffness surrounding the fault in both the shear‐parallel and fault‐normal directions, to investigate the origin of slip instabilities and changes in friction constitutive properties. We confirm the transition from stable sliding through slow slip to stick‐slip due to reduced fault‐parallel stiffness, and that the occurrence of different types of slip events can be explained by the ratio between shear and critical stiffness. In contrast, reducing the fault‐normal stiffness produces stick‐slip instabilities under conditions where the conventional critical stiffness criterion predicts stable sliding, and does not produce transitional slow slip events. Our data suggest that: (a) the stability criterion for frictional slip should be modified to incorporate fault‐normal stiffness, and (b) the unexpected slip instabilities may represent wrinkle‐like slip pulses, possibly due to a stiffness asymmetry introduced by lowering the fault normal stiffness on one side of the fault. This implies that earthquakes may occur when the fault‐normal stiffness, or bulk modulus for natural faults, is decreased and/or asymmetric across the fault zone, both of which may be common in nature. Plain Language Summary: Earthquakes occur on fault planes, but whether a fault will show stable sliding or host earthquakes, or their lab equivalent called "stick‐slip", depends only partly on the fault properties but also on how the fault and the surrounding rock respond to stress changes (the stiffness). Previous studies have shown that the transition between stick‐slip instabilities and stable sliding along a fault, can be triggered by changing the stiffness in the direction along the fault plane. However, faults commonly also have movement perpendicular to the fault plane, for example, if the fault surface is very rough, and therefore we study the effect of stiffness changes both perpendicular and parallel to the fault plane. We confirm previous observations when changing the stiffness parallel to the fault plane, but also observe unexpected stick‐slip instabilities when we decrease the stiffness perpendicular to the fault plane. This means that: (a) we have to adjust the stiffness criterion where stick‐slip instabilities are expected to include the fault‐normal stiffness, and (b) the unexpected stick‐slip instabilities at low fault perpendicular stiffness may be a different type of slip instability, called wrinkle‐like slip pulses, that may occur when one side of the fault is stiffer than the other. Key Points: Reduced fault‐normal stiffness alone can cause stick‐slip instabilities, but not slow slip in quartz gougeSystematically reducing both fault‐normal and shear‐parallel stiffness shows highly irregular slip eventsAdapting the critical stiffness criterion for fault‐normal stiffness shows slip instabilities are more likely [ABSTRACT FROM AUTHOR]

Purpose: Running at a given speed can be achieved by taking large steps at a low frequency or on the contrary by taking small steps at a high frequency. The consequences of a change in step frequency, at a fixed speed, affects the stiffness of the lower limb differently. In this study, we compared the running mechanics and kinematics at different imposed step frequencies (from 2 step s−1 to 3.6 step s−1) to understand the relationship between kinematic and kinetic parameters. Methods: Eight recreational male runners ran on a treadmill at 5 different speeds and 5 different step frequencies. The lower-limb segment motion and the ground reaction forces were recorded. Mechanical powers, general gait parameters, lower-limb movements and coordination were investigated. Results: At low step frequencies, in order to limit the magnitude of the ground reaction force, the vertical stiffness is reduced and thus runners deviate from an elastic rebound. At high step frequencies, the stiffness is increased and the elastic rebound is optimised in its ability to absorb and restore energy during the contact phase. Conclusion: We studied the consequences of a change in step frequency on the bouncing mechanics of running. We showed that the lower limb stiffness and the intersegmental coordination of the lower-limb segments are affected by running step frequency rather than speed. The runner rather adapts their lower limb stiffness to match a step frequency for a given speed than the opposite. [ABSTRACT FROM AUTHOR]

Purpose: The aim of this study was to investigate internal Achilles tendon (AT) displacement, AT shear wave velocity (SWV), and triceps surae (TS) muscle shear modulus in athletes. Methods: Internal AT displacement was assessed using ultrasound during isometric contraction. Shear wave elastography was used to assess AT SWV (m × s–1) at rest and TS muscle shear modulus (kPa) during passive ankle dorsiflexion. Results: A total of 131 athletes participated in this study. Athletes who had not exercised within two days had greater AT non-uniformity and mean anterior tendon displacement, and lower SWV at the proximal AT measurement site (mean difference [95% CI]: 1.8 mm [0.6–2.9], p = 0.003; 1.6 mm [0.2–2.9], p = 0.021; – 0.9 m × s–1 [– 1.6 to – 0.2], p = 0.014, respectively). Male basketball players had a lower mean AT displacement compared to gymnasts (– 3.7 mm [– 6.9 to – 0.5], p = 0.042), with the difference localised in the anterior half of the tendon (– 5.1 mm [– 9.0 to – 1.1], p = 0.022). Male gymnasts had a smaller absolute difference in medial gastrocnemius-minus-soleus shear modulus than basketball players (59.6 kPa [29.0–90.2], p < 0.001) and track and field athletes (52.7 kPa [19.2–86.3], p = 0.004). Intraclass correlation coefficients of measurements ranged from 0.720 to 0.937 for internal AT displacement, from 0.696 to 0.936 for AT SWE, and from 0.570 to 0.890 for TS muscles. Conclusion: This study provides a reliability assessment of muscle and tendon SWV. The relative differences in passive TS muscle shear modulus suggest sport-specific adaptation. Importantly, in healthy individuals, lower AT displacement after exercise may reflect the time required for tendon recovery. [ABSTRACT FROM AUTHOR]

This paper describes a new simple and inexpensive method for accurately measuring the three-dimensional deformation of soil specimens during triaxial tests. The method incorporates a cost-effective image capture system that can be applied to any triaxial apparatus as well as an automated post-image analysis approach for processing the images acquired during triaxial tests. Image capture is performed using multiple low-cost Raspberry Pi cameras and accessories placed outside the triaxial chamber. The system employs particle image velocimetry, camera calibration and stereophotogrammetry to determine the spatial variation in axial and radial sample strains as samples are loaded. It is demonstrated that axial and radial displacements can be measured with an accuracy of 1–2 μm, respectively, and it is verified by comparison with data from triaxial tests on sand samples that the full nonlinear stiffness–strain degradation of soil samples from fully elastic conditions can be determined without the need for any additional sensors. [ABSTRACT FROM AUTHOR]

Background: Peripheral nerve injuries (PNIs) represent a significant clinical problem, and standard approaches to nerve repair have limitations. Recent breakthroughs in 3D printing and stem cell technologies offer a promising solution for nerve regeneration. The main purpose of this study was to examine the biomechanical characteristics in muscle tissue distal to a nerve defect in a murine model of peripheral nerve regeneration from physiological stress to failure. Methods: In this experimental study, we enrolled 18 Wistar rats in which we created a 10 mm sciatic nerve defect. Furthermore, we divided them into three groups as follows: in Group 1, we used 3D nerve guidance conduits (NGCs) and adipose stem cells (ASCs) in seven rats; in Group 2, we used only 3D NGCs for seven rats; and in Group 3, we created only the defect in four rats. We monitored the degree of atrophy at 4, 8, and 12 weeks by measuring the diameter of the tibialis anterior (TA) muscle. At the end of 12 weeks, we took the TA muscle and analyzed it uniaxially at 10% stretch until failure. Results: In the group of animals with 3D NGCs and ASCs, we recorded the lowest degree of atrophy at 4 weeks, 8 weeks, and 12 weeks after nerve reconstruction. At 10% stretch, the control group had the highest Cauchy stress values compared to the 3D NGC group (0.164 MPa vs. 0.141 MPa, p = 0.007) and the 3D NGC + ASC group (0.164 MPa vs. 0.123 MPa, p = 0.007). In addition, we found that the control group (1.763 MPa) had the highest TA muscle stiffness, followed by the 3D NGC group (1.412 MPa), with the best muscle elasticity showing in the group in which we used 3D NGC + ASC (1.147 MPa). At failure, TA muscle samples from the 3D NGC + ASC group demonstrated better compliance and a higher degree of elasticity compared to the other two groups (p = 0.002 and p = 0.008). Conclusions: Our study demonstrates that the combination of 3D NGC and ASC increases the process of nerve regeneration and significantly improves the compliance and mechanical characteristics of muscle tissue distal to the injury site in a PNI murine model. [ABSTRACT FROM AUTHOR]

Horsley, BJ, Tofari, PJ, Halson, SL, Kemp, JG, Chalkley, D, Cole, MH, Johnston, RD, and Cormack, SJ. Validity and reliability of thoracic-mounted inertial measurement units to derive gait characteristics during running. J Strength Cond Res 38(2): 274-282, 2024--Inertial measurement units (IMUs) attached to the tibia or lumbar spine can be used to analyze running gait but, with teamsports, are often contained in global navigation satellite system (GNSS) units worn on the thoracic spine. We assessed the validity and reliability of thoracic-mounted IMUs to derive gait characteristics, including peak vertical ground reaction force (vGRFpeak) and vertical stiffness (Kvert). Sixteen recreationally active subjects performed 40 m run throughs at 3-4, 5-6, and 7-8 m·s21. Inertial measurement units were attached to the tibia, lumbar, and thoracic spine, whereas 2 GNSS units were also worn on the thoracic spine. Initial contact (IC) from a validated algorithm was evaluated with F1 score and agreement (mean difference 6SD) of gait data with the tibia and lumbar spine using nonparametric limits of agreement (LoA). Test-retest error {coefficient of variation, CV (95% confidence interval [CI])} established reliability. Thoracic IMUs detected a nearly perfect proportion (F1 $ 0.95) of IC events compared with tibia and lumbar sites. Step length had the strongest agreement (0 6 0.04 m) at 3-4 m·s21, whereas contact time improved from 3 to 4 (20.02860.018 second) to 7-8m·s21 (20.00460.013 second). All values for Kvert fell within the LoA at 7-8 m·s21. Test-retest error was #12.8% for all gait characteristics obtained from GNSS units, where Kvert was most reliable at 3-4 m·s21 (6.8% [5.2, 9.6]) and vGRFpeak at 7-8m·s21 (3.7% [2.5, 5.2]). The thoracic-spine site is suitable to derive gait characteristics, including Kvert, from IMUs within GNSS units, eliminating the need for additional sensors to analyze running gait. [ABSTRACT FROM AUTHOR]

Waste-tire textile fibers (WTTF) represent a challenge for the recycling industry since there are currently very few alternatives for their use. In this study, an evaluation of the effect of a new additive developed in two granular formats from WTTF on the fatigue behavior of asphalt mixtures was performed. For the first format of the WTTF-based additive, its effect was evaluated on hot-mix asphalt (HMA), while for the second format of the additive, the effects were evaluated on stone mastic asphalt (SMA). This second format represents an alternative that allows for the total replacement of the cellulose stabilizing additive used in the reference mix. The evaluation of fatigue damage in the mixes was performed using the four-point bending beam (4PB) test specified in European standard EN 12697-24. The test results show that the asphalt mixtures manufactured with WTTF-based additives exhibited a higher capacity to resist load cycles before failure compared to the reference mixtures. Likewise, once the asphalt mixtures were evaluated in a pavement structure by means of an empirical mechanistic analysis, the pavement structures composed of asphalt mixtures with WTTF-based additives showed significant improvements in their durability for the different load axes evaluated. For an average thickness of 15 cm of asphalt mix of a pavement-type structure, the use of the WTTF additive increases the durability of the structures by up to 129% and 112% compared to the HMA and SMA reference mixtures, respectively. These results show that both formats of the WTTF-based admixture improve the fatigue damage resistance of the HMA and SMA asphalt mixtures. [ABSTRACT FROM AUTHOR]

For mechanical metamaterials and their vibration isolation ability, a new corset type structure (CTS) is designed from the inward hexagonal steel structure by applying fillet at the inward corners. Ten CTS cells are born by using the different fillet radius. The fillet radius is 10 mm to 100 mm, but the cell mass remains constant when the plate has the same thickness. The static deformation, vibration modality and harmonic response of these NPR structures are analyzed in this paper. These CTS cells are modeled by using the finite element method (FEM) with a uniform grids. In static analysis, a surface load and a point load on the top plate are respectively considered to study the elastic deformation, the NPR and the stiffness of CTS cells with different fillet radii and thicknesses. These CTS cells have a greater NPR and a higher stiffness than the original inward hexagonal steel structure. In modal analysis, the natural frequency, the eigenmode and the fixed modality are numerically computed. These frequency values and displacement distributions of CTS cells show that these CTS cells have a higher vibration frequency than the origin inward hexagonal structure cell. In harmonic response analysis, the frequency domain is from 1 Hz to 1000 Hz, and the excitation force is on the top surface of the upper plate. All displacement responses of these CTS cells are analyzed. The harmonic response analysis result shows that the resonance magnitude can be significantly suppressed by these new CTS cells. The analysis result presents the characteristics of this new CTS, and it is beneficial for the vibration isolation in engineering application. [ABSTRACT FROM AUTHOR]

T-shaped reinforced concrete (T-RC) beams are widely used in high-rise buildings due to their advantages of reduced concrete usage, low sectional stress, and decreased weight of the structure. However, the calculation method of residual flexural stiffness of T-RC beams has limited existing research. To address this knowledge gap, a comprehensive experimental investigation was conducted, comprising fire tests, dynamic tests, and static load tests on 10 T-RC beams. The aim was to analyze the effects of fire time and load ratio on the residual flexural stiffness of composite beams. The experimental results showed that the residual flexural stiffness of T-RC beams decreased with increasing fire time and load ratio. However, the impact of fire time was more significant than the load ratio. A refined finite element model of T-RC beams under high temperature and after high temperature was established by modifying the frequency data of the dynamic test and considering the impact of cracks. The model accurately predicted the temperature field and deflection change of T-RC beams under fire. Furthermore, a method for calculating the residual flexural stiffness of T-RC beams considering the effect of fire was proposed and validated against the measured values. The proposed method demonstrated a low error rate of less than 10%. [ABSTRACT FROM AUTHOR]

The design of a large excavation using a braced wall system (BWS) with H-shaped steel (HS) struts is introduced. In situ axial loading tests were conducted to study the load transfer and deformation mechanisms of an HS strut with double splay supports. The monitoring results indicate that the outer splay support withstands the most lateral earth pressure (48.5%), followed by the inner splay support (35.6%) and BC segment of the end support (15.9%). The safety redundancies of strength and stability for the outer and inner splay supports, AB and BC segments of the end support are all larger than 1.59. The axial deformations of the middle support and the whole strut tend to increase linearly with the loading process. Using the monitored total load of hydraulic jacks, the value of KT″ of the whole strut is estimated to be 158–189 MN/m. The modified value of KT″ (KT″ba/S) of the whole strut is 2.2–2.6 MN/m. It is close to the lower limit of the suggested range (2.5–3.5 MN/m) in the Chinese standard. The monitored data of typical safety control parameters during soil excavation and the monitored results during in situ loading tests indicate good performance of the BWS with HS struts in this project. [ABSTRACT FROM AUTHOR]

This study proposed a method to characterize the effect of support system stiffness in excavation design based on a newly developed stiffness influence factor. Three-dimensional finite-element analyses were performed considering various excavation, support, and soil conditions. Focus was given to the effect of system stiffness in irregular excavation configuration with a re-entrant corner in different soil conditions. Wall deflection and surface settlement within the re-entrant corner zone were smaller due to the corner stiffening effect, yet deeper and wider influence zones of wall deflection and surface settlement were shown. The individual performance of the influence parameters was evaluated and introduced into a unified evaluation model, and the system stiffness influence factor was established. The proposed system stiffness influence factor was sufficiently effective in predicting wall deflection and surface settlement. Case examples showed that the proposed method is valid and effective for excavation design. The sensitivity analysis showed that the wall stiffness contributed most to the performance of the wall, which was applicable to further enhancing and optimizing excavation design. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Harbin Institute of Technology. Social Sciences Edition / Haerbin Gongye Daxue Xuebao. Shehui Kexue Ban is the property of Harbin Institute of Technology and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Designing materials that mitigate impacts effectively are crucial for protecting people and structures. Here, a single-resonator metamaterial with negative mass characteristics is proposed for impact mitigation, and numerical analysis of wave propagation shows explicitly how the spring stiffness and number of unit cells influence that mitigation. The results show clearly that a metamaterial with differing microstructural stiffness is better at mitigating the effect of a shock wave than one with a unique stiffness. Also, there is a critical number of unit cells beyond which the shock wave is not attenuated further, but the fabrication complexity increases. In the 40 groups of microstructural regions in this example, the attenuation effect no longer increases when there are more than 35 groups. This work offers guidance for microstructure designs in metamaterials and provides new ideas for using metamaterials to mitigate shock waves. [ABSTRACT FROM AUTHOR]

In the press and injection hybrid molded parts of fiber-reinforced thermoplastics (FRTPs), failure at the interface between the surface material (the outer shell) and the ribs (the injection part) or that at the injection part has become an issue. Adding a resin layer to the rib roots at the same time that the ribs are molded through injection has been proposed, which may increase the mechanical properties and reduce the material cost. To prevent failure at the injection part, the use of fiber-reinforced resin as an injection material has been suggested. This approach contributes to a higher bond strength by lowering the molding shrinkage rate. In this study, the hat-shaped parts of carbon fiber-reinforced thermoplastics (CFRTPs) with fiber-reinforced and neat resin layers at the rib root were fabricated through press and injection hybrid molding, and their mechanical properties were evaluated through three-point bending tests. The effects of the resin layer at the rib root and the existence or nonexistence of fiber reinforcement on the mechanical properties, as well as the relationship between the material cost and the mechanical properties, were clarified through an experiment and FEM analysis. The bond strength was also evaluated through tensile tests that were undertaken at the rib root. Molded parts with neat PA6 and glass fiber-reinforced PA6 resin layers at the rib roots showed higher bond strength than those without resin layers. In a three-point bending test of a CFRTP hat-shaped part with a resin layer at the rib roots, the use of a 1 mm thick CFRTP laminate for the outer shell and glass fiber-reinforced PA6 resin as the injection material showed the same stiffness as a part that used a 2 mm thick CFRTP laminate for the outer shell. FEM analysis showed that the resin layer prevented the concentration of strain at the rib roots, and the model that used a 1 mm thick CFRTP laminate for the outer shell and glass fiber-reinforced PA6 resin as the injection material showed the best specific stiffness in this study. By adding a resin layer to the rib roots, the fabrication of molded parts with excellent specific stiffness was enabled at a small increase in cost. [ABSTRACT FROM AUTHOR]