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This article has three interrelated aims: first, to examine the achievements of the study of the phenomenon of backpacker congregations as 'enclaves', and present the principal historical trajectory of those enclaves in terms of a sequence of transitions: from inception and enclavisation, to conventionalisation and eventual medialisation. Second, to offer a critique of the prevailing approach to backpacker enclaves and dwell upon its inherent deficiencies. Finally, to suggest an alternative approach to backpacker congregations, based on the concept of 'assemblages', for a more adequate and nuanced representation of the spatial and virtual processes, by which backpacker enclaves are constituted, and to explicate their multiple enmeshments in their proximal and distal environment. [ABSTRACT FROM AUTHOR]

Background: Giardia duodenalis is a parasitic protist that infects a large number of species, being localized in the small intestine. Two of the eight recognized assemblages have zoonotic potential, but studies regarding their distribution in less important pet or farm species are scarce. Of these species, the long-tailed chinchilla is a host for Giardia spp., although data on the spread of infection and assemblages involved are confined. The present work aimed to determine the prevalence of Giardia infection and assemblage identification in farmed chinchillas in Romania. A total of 341 fecal samples were collected from 5 farms and microscopically examined using flotation test based on saturated sodium chloride solution. DNA from all positive samples was extracted and identified by PCR targeting the gdh gene. Results: The overall prevalence of Giardia infection was 55.7% (190/341); there was no statistically significant difference (P = 0.25) in prevalence between young animals (58.8%) and adults (52.6%). Assemblages B (151/190), D (33/190) and E (6/190) were identified. Among assemblage B, sub-assemblages BIII (6/151) and BIV (145/151) were determined. Conclusions: This study demonstrates that Giardia spp. infection is highly prevalent in farmed chinchillas from Romania, and the sub-assemblages identified are potentially zoonotic. [ABSTRACT FROM AUTHOR]

What a macro-history of containers look like? Containers are not really a bounded category of thing; they are a relational technology which transforms things into assemblages. Their history is thus that of an unbounded history of relations between things, rather than a bounded history of discrete objects. [ABSTRACT FROM PUBLISHER]

This article assesses the concepts of militarism and militarization in relation to contemporary security interventions in the Sahel, a region increasingly understood through the prisms of violence, cross-border illicit flows, and limited statehood. This region is subject to security interventions that include French military action, EU-funded projects to prevent drug trafficking, and both bilateral and multilateral efforts against irregular migration. To many observers, it is experiencing an ongoing militarization. We argue that while the inextricable concepts of militarism and militarization go some way towards explaining interventions’ occasional use of military violence, they are limited in their grasp of the non-martial and symbolic violence in security practices. We instead propose a focus on assemblages of (in)security to show the heterogeneous mix of global and local actors, and often contradictory rationalities and practices that shape the logics of symbolic and martial violence in the region. Throughout, the article draws on the authors’ fieldwork in Mauritania, Senegal, and Niger, and includes two case studies on efforts against the Sahel’s ‘crime–terror nexus’ and to control irregular migration through the region. The article’s contribution is to better situate debates about militarism and militarization in relation to (in)security and to provide a more granular understanding of the Sahel’s security politics. [ABSTRACT FROM AUTHOR]

Infections with the zoonotic endoparasite Giardia duodenalis are widely spread among dogs and cats worldwide. Since the question whether the infection might be transmitted from domestic animals to their owners is still an important topic, a reliable detection of patent Giardia infections and the determination of the associated Giardia assemblages is of major concern. The objectives of the present study were to determine the prevalence of Giardia infections in dogs and cats living in Germany using different diagnostic tests and to identify the Giardia assemblages of infected animals. Furthermore, a possible correlation of coinfections with other endoparasites was analysed. All samples were investigated by enzyme-linked immunosorbent assay (ELISA), merthiolate-iodine-formalin concentration technique (MIFC) and zinc chloride flotation. ELISA-positive samples were additionally screened with a direct immunofluorescence assay (IFA). Faecal DNA was extracted from all Giardia cyst-positive samples and used for multilocus sequence typing with nested PCRs targeting the following gene loci: SSU rRNA (SSU), glutamate dehydrogenase (gdh) and triosephosphate isomerase (tpi). Samples from dogs and cats tested positive for Giardia coproantigen (ELISA) in 30.6% and 17.9%, respectively. The MIFC technique revealed Giardia cysts in 33.9% of canine and in 34.6% of feline ELISA-positive samples, while using IFA, cysts were present in 90.4% of canine and in 76.9% of feline ELISA-positive samples. Coinfections with other endoparasites besides Giardia were found in both dogs and cats, yet a statistically significant correlation could solely be drawn for the canine samples. The success rate of the different PCR protocols varied between 23.1% (tpi) and 91.3% (SSU) for dogs and between 25.0% (gdh) and 90.0% (SSU) for cats. Dog-specific Giardia assemblages C and D were detected in 42 and 55 canine isolates, respectively. The cat-specific Giardia assemblage F was detected in 14 feline isolates. Two canine and two feline samples harboured the zoonotic assemblage A. According to the results of the study, Giardia is a common endoparasite in dogs and cats from Germany. The exclusive application of MIFC is insufficient for a reliable identification of patent Giardia infections since the IFA revealed a higher sensitivity for the detection of Giardia cysts in feline and canine faecal samples. Even though the majority of investigated animals harboured the species-specific Giardia assemblages C, D and F, a zoonotic potential arising from assemblage A could not be excluded. [ABSTRACT FROM AUTHOR]

Drawing from stories told by migrant women in Hong Kong, this article builds on previous studies of 'left-behind children' and calls for greater attention to the spectrum of sorts of absent children and to the formation of queer or less normative forms of migratory families. Taking a two-pronged approach, I present an on-the-ground ethnographic and affective approach through several vignettes, and consider key elements of a more mid-range and distanced 'global assemblage' approach to the institutions and expert knowledge that shape the experiences and practices of migrant mothers, migratory families, and the spectrum of absent children. This article posits that one's biological children, perhaps the most familial of kin, can become familiar or even unfamiliar strangers through contemporary processes, technologies and practices of migration and separation, and that the process of migration makes and unmakes conventional and unconventional sorts of families. While affective and assemblage approaches are independently valuable, combined they offer richer understandings of the complex interplay of factors - at various levels - that shape normative and queer families and different types of children's absences. [ABSTRACT FROM AUTHOR]

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At a time of increasing calls from policy makers for the use of ‘hard evidence’ in driving decision-making at national and local levels in educational contexts, this article contributes to debates about evidence-based practice in early literacy research. It proposes that a reliance on studies designed to generate ‘hard’ evidence limits understanding about innovations and interventions, arguing that such reliance is not just problematic because interventions and innovations are interpreted differently in diverse sites, or because programmes need to be locally relevant, but because they are constituted differently through different evaluation studies. The article draws on Law’s notion of ‘method assemblage’ to consider how different studies produce different assemblages that have implications for how innovations are conceived. These ideas are exemplified using studies scrutinised through a systematic literature review of one kind of literacy intervention, early years book-gifting, which aims to promote book-sharing in the home. The discussion focuses specifically on how books as mediating objects are instantiated in various ways through different studies, with different implications for how book-sharing, book-gifting and, ultimately, reading are understood. When considered together, these studies construct book-gifting in multiple ways, problematising and complicating the causal relations assumed in methodologies driving for ‘hard’ evidence. Drawing on the book-gifting example, this article explores what might be gained by embracing ‘multiplicities’, the multiple ways in which things – such as objects, activities, principles and indeed literacy interventions – are constituted through method assemblage. It argues that literacy evaluations can best serve children and their families, and the organisations, agencies and groups working alongside them, by seeking fluid, open and ‘generous’ accounts of innovations and interventions. Such accounts, it is argued, are more likely to acknowledge the complex relationships and practices associated with early literacy and to generate new understandings and productive possibilities for early literacy learning. [ABSTRACT FROM AUTHOR]

Focusing on digital text messages containing information about students' absences and sent to parents by schools, the paper investigates the way school absenteeism is produced within affective assemblages. The paper unfolds a theoretical and methodological approach of 'going with' the text messages, in entanglements of affective flows. The empirical engagements, produced together with multiple agents in two Swedish schools, show that within the assemblages of human and nonhuman bodies, the text messages can become 'stirrers' that evoke nervousness and anxiety, but also excitement and feelings of control that affect the production and conception of absenteeism. The affective flows of text messages thus travel in all directions, with and against notions of linearity. The conclusion emphasizes how the text messages, as affective materialities, are an inextricable part of the production of school absenteeism in multiple and sometimes unexpected ways. [ABSTRACT FROM AUTHOR]

Most armed conflict today takes place within urban terrain or within an urbanised context. An extreme variant of such armed conflict is violence perpetrated by external state and non-state forces within the city, known as urbicide. Urbicidal violence deliberately strives to kill, discipline or deny the city to its inhabitants by targeting and then reordering the sociomaterial urban assemblage. Civil resistance within urbicidal violence seeks to subvert the emerging alternative sovereign order sought by such forces. It does so by using the inherent logic of the city in order to maintain/restore the community’s social cohesion, mitigate the violence, affirm humanity, and claim the right to the city. This paper investigates the city-logic of civil resistance through examples drawn from the recent urbicidal experiences of Middle East cities such as Gaza, Aleppo, Mosul, and Sana’a. Theoretical insights from the conflict resolution literature, critical urban theory, and assemblage thinking inform the argument. [ABSTRACT FROM AUTHOR]

• We use qualitative research to locate the everyday affects of transport in Dhaka. • Lived experiences of safety are entangled in human & non-human elements of travel. • Understanding power of affects in transport can help reconfigure safety for women. By examining the lived experiences of 30 female bus commuters in Dhaka using in-depth qualitative approaches, this paper argues for an enhanced understanding of socio-cognitive undercurrents of gendered mobilities. By privileging a feminist-affective lens, and tracing the emotionally and politically charged everyday negotiations of space, power struggles, (dis) comfort, and encounters between gendered bodies, the paper contends that women's agency to act and respond to harassment in public transport is contingent on multi-scalar assemblages comprising socio-technical infrastructures, lifestyles, cultural histories, personal dispositions and situated knowledge. Moreover, by applying assemblage thinking and affect theories in transport spaces, the study links discussions on gender, violence and mobility beyond the common economic tropes as is common in transport studies of the Global South. [ABSTRACT FROM AUTHOR]

This article takes the case studies of music and clothing collections in the home to explore the possibilities for developing comparative research into everyday consumption by focusing upon personal collections. Drawing on two empirical research projects, it challenges dominant understandings of collections as ‘special’ or separated off from daily practices by considering music and clothing collections as the site for everyday consumption practices as well as the locus of memories. Collections are reframed as ‘assemblages’ to explore the diverse materialities and temporalities that constitute the collections. Agency is distributed through the assemblage which allows for a problematisation of notions of individual consumer choice as the article explores the logics of the collections themselves. Focusing upon ‘collections’ paves the way for comparative work on different genres of consumption and to explore the diverse materialities of things and their relationalities. It widens our understanding of consumption to incorporate the use of things both in the enactment of daily life and which are kept or stored. [ABSTRACT FROM AUTHOR]

We studied the effects of the overstory canopy cover on ground-dwelling harvestmen communities in European beech forests in the Western Carpathian Mts. We analyzed the differences in species richness, abundance and composition in two tree canopy cover categories (closed and open canopy). Overall, 1765 individuals belonging to 16 species were caught using pitfall traps. Repeated-measures ANOVA revealed that under both closed and open canopies a similar absolute and rarefied species richness and number of individuals (standardized to 100 pitfall trap days) were caught, and that both species richness and abundance were affected by the season (i.e., time of trap replacement); yet the total number of individuals trapped was more than two times greater under the open canopy than under the closed canopy. Despite the subtle differences in the taxonomic composition of the catches under the open and closed canopies revealed by DCA followed by RDA, the composition of ground-dwelling harvestmen assemblages differed under the two canopy covers. The open canopy was more suitable for heliophilic, xerothermic eurytopic harvestmen species typical for open and ecotonal habitats, such as Egaenus convexus (C.L. Koch) and Oligolophus tridens (C.L. Koch) but still suitable for hemihygrophilic Lophopilio palpinalis (Herbst). The closed canopy stands were preferred by shade-tolerant, hygrophilic eurytopic harvestmen species, such as Trogulus sp. Latreille, Dicranolasma scabrum (Herbst) and Platybunus bucephalus (C.L. Koch). Our results highlight the importance of intra-habitat heterogeneity of a harvestmen community. [ABSTRACT FROM AUTHOR]

In this essay, we argue that assemblage thinking has much to offer geographers attempting to conceptualize the interrelationships between sexualities, subjectivities and contemporary urban spaces. In our current research on queer mobilities, we found that despite the explanatory weight provided by mobilities approaches, two fundamental questions remained: how to conceptualise the nature of ‘the queer subject’, and how to better explain why certain kinds of urban spaces still seemed to support these newly mobile queer subjects. Here we speculate on the potential of assemblage thinking to formulate some new theoretical and conceptual responses to these questions. While quite preliminary, there is the potential to understand how interlocking (but temporary and unstable) notions of identities, subjectivities and mobilities (of ideas, people, knowledge, capital, etc.) can be ‘assembled’ in and across urban spaces, recursively reshaping sexualities and places. [ABSTRACT FROM AUTHOR]

Community composition reflects evolutionary and ecological processes such as diversification and species assortment. Communities are generally considered to be saturated, which means that the number of species is maximized and that regulatory mechanisms, such as interspecific competition, prevent the addition of new species. In the tropics, however, species numbers of local bird assemblages double up each winter after the arrival of migratory species, which suggests that a rearrangement of niches occurs. Here ecological space is defined by morphological traits that reflect the ecological position of species, to describe changes in the structure of local communities. We found that the average morphological distance between species was reduced, and the volume (the total extent of trait space) was expanded with the arrival of migrants, indicating that newcomers occupy the periphery and the interior of the available ecological space. Furthermore, with greater morphological differences between resident species, higher numbers of migratory species can take advantage of free space and potentially exploit unused resources due to their intermediate morphology. We highlight the differences in the attributes of resident versus migratory species that may allow both to be accommodated in the morphological space, and we suggest coexistence theory as a possible explanation for the co-occurrence of similar species. [ABSTRACT FROM AUTHOR]

This article draws from data of a year-long qualitative study on the lived experiences of genderqueer individuals. Two critical questions helped aide in thinking about the lived experiences of genderqueer individuals: (1) What does it mean to claim a genderqueer identity? and (2) How is genderqueer experienced, embodied, and understood? The principal work of José Muñoz, Gayle Salamon, Gilles Deleuze and Felix Guattari, and Jasbir Puar was used to support the theoretical frame and qualitative data analysis. What makes a person genderqueer is not clearly defined, but is organic and personal, and brings forth negotiations of social and felt sense of gender, and internal and external oppression. Specifically, the ways in which genderqueer individuals negotiate their identity related to the social construction of gender, the felt sense of gender, and gender as becoming are analyzed and discussed. [ABSTRACT FROM PUBLISHER]

The strength and importance of consumer effects are predicted to increase toward low latitudes, but this hypothesis has rarely been tested using a spatially consistent methodology. In a consumer-exclusion experiment spanning twenty degrees of latitude along the east Australian coast, the magnitude of consumer effects on sub-tidal sessile assemblage composition was not greater at low than high latitudes. Across caged and control assemblages, Shannon's diversity, Pielou's evenness, and richness of functional groups decreased with increasing latitude, but the magnitude of consumer effects on these metrics did not display consistent latitudinal gradients. Instead, latitudinal gradients in consumer effects were apparent for individual functional groups. Solitary ascidians displayed the pattern consistent with predictions of greater direct effects of predators at low than high latitude. As consumers reduced the biomass of this and other competitive dominants, groups less prone to predation (e.g., hydroids, various groups of bryozoans) were able to take advantage of freed space in the presence of consumers and show increased abundances there. This large-scale empirical study demonstrates the complexity of species interactions, and the failure of assemblage-level metrics to adequately capture consumer effects over large spatial gradients. [ABSTRACT FROM AUTHOR]

It is estimated that faecal-orally transmitted diseases are common in Afghanistan, as a consequence of poor hygienic standards of life and widespread contamination of water and food with both human and animal faeces. However, there is little information in the literature concerning infections caused by intestinal parasites in the Afghan population. In this study, we report the occurrence of Giardia intestinalis assemblages (A and B) in formalin-fixed stool samples collected from 245 Afghan schoolchildren living in Ghazni Province in eastern Afghanistan. Detection of the parasite's DNA and genotyping was performed using real-time PCR, specific to the β-giardin gene of G. intestinalis. Positive results were recorded in 52 (21.2%) samples. Genotyping was successful in 39 faecal samples and showed the predominance of assemblage B of G. intestinalis in this population (15 assemblage A and 24 assemblage B). Co-infection with both genotypes A and B was detected in four samples. Additionally, we evaluated the effect of 10% buffered formalin fixative on the detection of G. intestinalis DNA using real-time PCR and nested PCR characterised by different lengths of PCR products (74 and 479 bp, respectively). The human faeces containing the Giardia cysts were tested for 16 weeks. Amplification of G. intestinalis DNA with real-time PCR was possible up to 6 weeks of preservation of stool sample in formalin, compared to only 2 weeks with nested PCR. This suggests that real-time PCR is a more suitable tool in cases where stool samples have to be kept in formalin for longer periods of time. [ABSTRACT FROM AUTHOR]

Existing research on the formation of Asian medical travel destinations has highlighted a variety of activities that attract and accommodate patients from abroad. This paper contributes to the literature by drawing insights from an Indian case study, a major transnational health-care hub in Asia that has gained little scholarly attention thus far. Using connectivity as an analytical lens, we understand medical travel destinations as a contingent product of relating, connecting and assembling. We study how connectivity is embodied and how it unfolds in care encounters at corporate hospitals in the capital of New Delhi and surrounding urban areas. The following entities are the most effectual in the networks that constitute medical travel destinations in the National Capital Region: (i) circulating narrations of personal experiences; (ii) language interpreters; and (iii) commission fees. We further elaborate on how these connectors work to link foreign patients with Indian hospitals and how they affect itineraries as well as patients and other involved actors. Finally, we suggest that the approach deployed provides a suitable framework for future research aimed at gaining a better understanding of the wider impacts of medical travel by following these connections and examining their workings at places both close and distant. [ABSTRACT FROM AUTHOR]

Giardia lamblia is an intestinal parasite that has an extensive genetic variation among isolates. This species is divided into eight different assemblages (A–H), but only assemblages A and B have been associated with human infections. Studies on the associations of G. lamblia assemblages and symptoms have been done but were inconclusive. The aim of this study was to correlate G. lamblia assemblages with symptoms in patients with and without HIV/AIDS and its association with the CD4 T cell count. The cross-sectional survey was conducted among patients attending the Evandro Chagas National Institute of Infectious Diseases (INI/FIOCRUZ) in Rio de Janeiro from January 2011 to February 2015. Thirty-eight of 65 microscopically positive stool samples for G. lamblia were from HIV positive patients and 27 were from HIV negative patients. Of the HIV infected patients, 19 (55.9%) were genotyped as assemblage B of which 9 (47.4%) had a CD4 T cell count below 200 cells/mm 3 . In addition, we found a greater number of samples belonging to assemblage B in symptomatic cases (11 of 19; 57.9%). Our data suggest that assemblage B is very likely to be found in HIV infected patients and probably the lower CD4 T count gives advantages for assemblage B replication. Furthermore, assemblage B seems to be associated with symptomatology, particularly abdominal pain, asthenia, diarrhea, fever, headache and myalgia. This study provides information on G. lamblia assemblages and symptoms in patients with and without HIV/AIDS virus and their association with CD4 T cell counts. [ABSTRACT FROM AUTHOR]

This paper asks what translanguaging could start to look like if it incorporated an expanded version of language and questioned not only to the borders between languages but also the borders between semiotic modes. Developing the idea of spatial repertoires and assemblages, and looking at data from a Bangladeshi-owned corner shop, this paper suggests on the one hand that it is important to include a wide range of semiotic possibilities in any analysis. On the other hand, however, we cannot merely add more semiotic items to our translinguistic inventories, but need instead to seek out a way of grasping the relationships among a range of forms of semiosis. The notion of assemblages allows for an understanding of how different trajectories of people, semiotic resources and objects meet at particular moments and places, and thus helps us to see the importance of things, the consequences of the body, and the significance of place alongside the meanings of linguistic resources. [ABSTRACT FROM AUTHOR]

Regions or regional development have not only gained importance in spatial planning, but also got fuzzy meanings. Originally regions refer to a specific geographical demarcated area, often with an inductive historic or cultural meaning. In the meantime, in our networked world, the concept also refers to transport regions based on models of daily urban systems, or functional regions based on distinctive urban or social functions. Within the post-industrial era, the concept also gained importance as economic regions. In this paper, we will explore how regional concepts emerge out of specific economic interest concerning technological innovation. We will focus on a cross-border municipal case, for example, the triangle Eindhoven–Leuven–Aachen. With the help of the assemblage and actor-network theory, we will show how the identity of regions is translated by evolving actor-networks, and how these actor-networks are influenced and adapted by those alternating regional identities themselves. From these insights, we will come up with some ideas and propositions how regional planning could play an important role within these co-evolving processes. [ABSTRACT FROM PUBLISHER]

Ecosystem engineers can influence biodiversity by enhancing complexity, and modifying the availability of resources. Understanding the mechanisms by which ecosystem engineers shape biodiversity is central to the concept of ‘ecological engineering’ of anthropogenic structures to enhance biodiversity. Here the presence and complexity of artificial turf was manipulated on an artificial structure to test the hypothesis that the colonisation of sessile invertebrates and mobile epibiota would vary with habitat complexity. Both sessile and mobile assemblage compositions differed according to the presence of artificial turf, and its complexity. Sessile invertebrates occupied greater proportions of available space on topographically simple ‘blank’ surfaces or low complexity artificial turf than those with high complexity turf, whereas mobile taxa were generally more abundant on the turf. However, the mobile assemblage was unrelated to the sessile assemblage when examined within each level of initial substrata complexity. Contrary to the increasing number of studies demonstrating nested hierarchical relationships between co-occurring ecosystem engineers, this study provides an example of an ecosystem engineer mimic (artificial turf) leading to the formation of habitat mosaics at small scales. The introduction of complex substrata to otherwise topographically simple artificial structures is a promising means of actively influencing assemblage composition. [ABSTRACT FROM AUTHOR]

'Third wave' neo-charismatic evangelical discourses of spiritual warfare envision the world as caught within a struggle between good and evil, in which demonic forces play an active role in shaping the lives of individuals, institutions, and nations. In contemporary American spiritual warfare discourse one demonic spirit has gained particular notoriety: the Jezebel spirit. Through a close reading of American spiritual warfare manuals, this article explores constructions of the Jezebel spirit and her place in third wave demonology. Constructed as a spiritual force reigning over an errant United States, the figure of Jezebel facilitates a discursive conflation of personal and social bodies in which the 'present absences' of 'deviant' (gendered, sexualised, racialised) bodies within the nation become figured as threatening to both national and spiritual survival. Drawing on poststructuralist, postcolonial, and queer theory, the article unpacks how Jezebel is constructed as a figure of feminised absence and multiplicity, whose 'illegitimate' possession of 'deviant' places and persons renders them as territories of absence that must be restored to normative presence through the reinscription of God's will. [ABSTRACT FROM AUTHOR]

This paper explores the market-making devices behind Bridge International Academies: a for-profit education company aiming to school millions of nursery and primary aged-students living on less than $2 per day. A wide variety of devices are utilised by Bridge International Academies to construct mass markets for low-cost schooling, including GPS devices that map low-income communities, smartphones that automate administrative functions, and computer devices that perform the duties of a teacher. Moreover, this paper also outlines the network of investors supplying the company with the necessary capital to put market devices into practice and hence, build markets for low-cost schooling. [ABSTRACT FROM AUTHOR]

This article proposes a reconceptualization of the term "actor" within motion pictures and presents the argument that "acting" is a matter of distributed agency performed by heterogeneous assemblages. What constitutes an actor is what I will label as a "cinematic actor assemblage," a term that comprises what is commonly known as human actors as well as material entities that play an active part in motion picture images. The use of intra-diegetic cameras in contemporary found footage horror films constitutes a particular case of such cinematic actor assemblages. Through a dynamic relational performance, cameras here take on roles as active agents with the potential to affect other elements within the images as well as the films' audiences. In found footage horror the assemblage mode of operation creates suspense, since the vulnerability of the camera threatens the viewer's access to the depicted events. While human characters and individual entities making up the camera assemblage are disposable, the recording is not. Found footage horror crucially hinges upon the survival of the footage. I will further suggest that these films allow filmmakers to experiment with the acting capabilities of intra-diegetic cameras. [ABSTRACT FROM AUTHOR]

Populations trends of cave-dwelling bats in the Eastern Iberian Peninsula and the effect of placing a perimeter fencing around their roosts (to avoid human disturbance on breeding colonies) were evaluated from 1997 to 2014. The species with the highest relative abundance was Miniopterus schreibersii (62.4%), followed by Myotis myotis/ blythii (18%), and both populations showed positive trends. On the other hand, Myotis capaccinii (6.2%), M. escalerai (4.8%) and M. emarginatus (0.9%) showed significant, but minor increases, particularly in recent years. Rhinolophus mehelyi (0.2%) displayed no significant trends, while a moderate population decline was recorded for R. euryale (5.1%). Rhinolophus ferrumequinum (2.2%) and R. hipposideros (0.1%) showed positive growth trends. The main assemblages in the evaluated roosts were formed by Myotis myotis/ blythii, Miniopterus schreibersii and R. ferrumequinum. This denotes their less specific requirements or greater flexibility when selecting roosts, compared with other species, except for M. capaccinii and R. mehelyi. No significant differences were found between roosts with different levels of protection, but there were positive trends in the protected roosts. Most non-fenced cavities showed negative trends during the period evaluated. We did not rule out other factors, such as requiring habitats with optimum food sources next to maternal roosts, which could affect population growth. The selectivity of some species of bats for certain caves will be essential for the preparation of management plans for certain roosts. Four of the seven risk factors documented for European bats affect studied population. Myotis blythii, M. myotis and Rhinolophus euryale would be most affected by a reduction in the areas they currently occupy. [ABSTRACT FROM AUTHOR]