301. Immunoliposomal systems targeting primary effusion lymphoma: in vitro study.
- Author
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Ruozi B, Riva G, Belletti D, Tosi G, Barozzi P, Luppi M, Forni F, and Vandelli MA
- Subjects
- Base Sequence, Cell Line, Tumor, Electrophoresis, Agar Gel, Flow Cytometry, Fluorescein-5-isothiocyanate, Humans, In Vitro Techniques, Lymphoma, Primary Effusion immunology, Lymphoma, Primary Effusion pathology, Microscopy, Atomic Force, Microscopy, Confocal, Liposomes, Lymphoma, Primary Effusion therapy
- Abstract
Aims: To develop an appropriate liposomal formulation for gene delivery against primary effusion lymphoma (PEL), a herpesvirus HHV8-associated B-cell lymphoma., Materials & Methods: Cationic, cationic PEGylated and cationic PEGylated anti-CD138 liposomes (ILp) linking a monoclonal antibody expressed on PEL cells were prepared by a thin layer evaporation method, followed by extrusion technique. The formulations were mixed with a model oligonucleotide to form the lipoplexes and tested on a BCBL-1 cell (a PEL cell line). The transfection efficiency was evaluated by flow cytometry and confocal laser scanning microscopy analysis., Results: Based on antigen-antibody interaction, ILp mediated a specific gene delivery as shown by a significant increase in the transfection rate and a localized internalization of the oligonucleotide, in comparison with cationic liposomes and cationic PEGylated liposomes., Conclusion: ILp could be proposed as effective carriers for oligonucleotide transfer in BCBL-1 cells. In vitro experimental results encourage us to further test the in vivo therapeutic potential of ILp for specific delivery of antitumoral agents.
- Published
- 2010
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