401. Methionyl-transfer ribonucleic acid deficiency during G1 arrest of Saccharomyces cerevisiae
- Author
-
M W Unger
- Subjects
S-Adenosylmethionine ,Cell division ,Mutant ,Saccharomyces cerevisiae ,Methionine-tRNA Ligase ,Microbiology ,Amino Acyl-tRNA Synthetases ,chemistry.chemical_compound ,Methionine ,RNA, Transfer ,Methionine synthase ,Thermolabile ,Molecular Biology ,Crosses, Genetic ,biology ,Structural gene ,Temperature ,Cell cycle ,biology.organism_classification ,Phenotype ,chemistry ,Biochemistry ,Genes ,Mutation ,biology.protein ,Cell Division ,Research Article - Abstract
The mesl- mutants of Saccharomyces cerevisiae cease division and accumulate in the G1 interval of the cell cycle when deprived of methionine or shifted from 23 to 36 degrees C in the presence of methionine. Synchronous cell cycle arrest results from a deficiency of charged methionyl-transfer ribonucleic acid (methionyl-tRNAMet) as shown by direct measurement of the in vivo pools of methionine, S-adenosylmethionine, and methionyl-tRNAMet. The deficiency of methionyl-tRNAMet in these cells is the consequence of a lesion in a single gene, mes1. mes1 appears to be the structural gene for the methionyl-tRNA synthetase because some revertants of this mutation exhibited a thermolabile methionyl-tRNA synthetase in vitro. A sufficient hypothesis to explain these and previous results is that the control of cell division by S. cerevisiae in response to nutrient limitation is mediated through aminoacyl-tRNA or subsequent steps in protein biosynthesis.
- Published
- 1977