2,581 results on '"Lan Jiang"'
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402. Heparin mimetics as potential intervention for COVID-19 and their bio-manufacturing
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Lan Jiang, Tianji Zhang, Hongzhong Lu, Saijuan Li, Kangjie Lv, Alex Tuffour, Lixin Zhang, Kan Ding, Jin-Ping Li, Hongmei Li, and Xueting Liu
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Infectious Medicine ,Bio-production ,Structural Biology ,Heparin ,Biological activities ,Genetics ,Biomedical Engineering ,Infektionsmedicin ,Biosynthesis ,Applied Microbiology and Biotechnology - Abstract
The COVID-19 pandemic has caused severe health problems worldwide and unprecedented decimation of the global economy. Moreover, after more than 2 years, many populations are still under pressure of infection. Thus, a broader perspective in developing antiviral strategies is still of great importance. Inspired by the observed multiple benefits of heparin in the treatment of thrombosis, the potential of low molecular weight heparin (LMWH) for the treatment of COVID-19 have been explored. Clinical applications found that LMWH decreased the level of inflammatory cytokines in COVID-19 patients, accordingly reducing lethality. Furthermore, several in vitro studies have demonstrated the important roles of heparan sulfate in SARS-CoV-2 infection and the inhibitory effects of heparin and heparin mimetics in viral infection. These clinical observations and designed studies argue for the potential to develop heparin mimetics as anti-SARS-CoV-2 drug candidates. In this review, we summarize the properties of heparin as an anticoagulant and the pharmaceutical possibilities for the treatment of virus infection, focusing on the perspectives of developing heparin mimetics via chemical synthesis, chemoenzymatic synthesis, and bioengineered production by microbial cell factories. The ultimate goal is to pave the eminent need for exploring novel compounds to treat coronavirus infection-caused diseases.
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- 2023
403. High Occurrence of Multiple-Drug Resistance Mediated by Integron in Aeromonas Isolated from Fish-Livestock Integrated Farms
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Yu Ting Deng, Yong Yong Feng, Ai Ping Tan, Fei Zhao, Lan Jiang, and Zhi Bin Huang
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Article Subject ,Aquatic Science - Abstract
Fish-livestock integrated farming is a traditional practice in South China and Southeast Asia. The administration of antimicrobial agents in livestock may potentially facilitate the spread of antimicrobial resistance to aquaculture through the drainage of livestock manure. Aeromonas spp. are fish pathogens that are the predominant bacteria in water bodies. The aim of this study was to characterize multiple-drug-resistantAeromonas spp. isolated from integrated and nonintegrated fish-livestock farms and to investigate the occurrence of class 1 integrons. Of the 481 Aeromonas strains examined in this study, isolates from pig manure, fish, and environmental samples from pig-fish or goose-fish integrated farms were significantly more resistant to various antimicrobial agents than those from nonintegrated farms P < 0.05 . High rates of resistance to nalidixic acid and sulfamonomethoxine were observed in the isolates from the integrated farm. Fifty Aeromonas isolates (10.4%) from integrated farms contained class 1 integrons. In addition, 96.0% (48/50) of the integron-positive strains displayed multiple-drug resistance. Ten types of gene cassette arrays were determined by sequencing, including dfrA17, dfrA12-orfF-aadA2, catB8, dfrB4-catB3-aadA1, aac6(6′)-Ib-cr-arr-3, aac-II-blaOXA-21-catB3, aar2-aacA4-drfA1-orfC, aac(6′)-Ib-cr, dfrA15, and dfrB4-catB3-blaOXA-10-aadA1. Notably, among the 50 integron-positive isolates, twenty isolates showed integrons located in plasmids, which may facilitate the transmission of resistance in integrated farms. Our investigation confirmed the high prevalence of multiple antimicrobial resistances mediated by class 1 integrons in Aeromonas isolates from integrated farms. Therefore, it is necessary to establish a risk assessment method for antimicrobial resistance in aquaculture.
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- 2023
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404. Ascodesmis rosicola sp. nov. and Talaromyces rosarhiza sp. nov., two endophytes from Rosa roxburghii in China
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Hong Zhang, Tian-Peng Wei, Yu-Tao Mao, Ming-Xia Ma, Kai Ma, Ying Shen, Mei-Juan Zheng, Wei-Yu Jia, Ming-Yan Luo, Yan Zeng, Yu-Lan Jiang, and Guang-Can Tao
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China ,Rosoideae ,Agriculture and Forestry ,Asia ,QH301-705.5 ,Rosa ,new taxa ,Pezizales ,Magnoliopsida ,taxonomy ,Central Asia ,Ascomycota ,Rosa multiflora ,Pezizomycetes ,morphology ,Aspergillaceae ,Rosales ,Biology (General) ,Plantae ,Rosaceae ,Ecology, Evolution, Behavior and Systematics ,Fungiculture ,endophytic fungi ,Rosa roxburghii ,Ecology ,Cenozoic ,Fungi ,tax ,Eurotiales ,Biota ,Eurotiomycetes ,Tracheophyta ,Talaromyces ,Ascodesmidaceae ,multigene phylogenetics ,Neogene ,Taxonomic Paper ,Ascodesmis - Abstract
Rosa roxburghii Tratt., a deciduous shrub of the family Rosaceae, is usually used as food and medicinal materials and also cultivated as an ornamental. Plant endophytic fungi are a large class of microbial resources not fully researched, with great potential applications. Two strains of Ascodesmis and Talaromyces were isolated during a survey of biodiversity on endophytic fungi of R. roxburghii in China. Multigene phylogenetic analyses showed that each of the two fungi formed a distinct lineage and separated from known congeneric species and they are proposed as two novel taxa. Ascodesmis rosicola sp. nov. usually has one or two conspicuous simple or branched ridges extending to the majority of the ascospore surface and remarkably small asci, distinguishing it from the previously-described species in the genus Ascodesmis. Talaromyces rosarhiza sp. nov., of the section Talaromyces, is closely related to T. francoae. It differs from the latter by having both monoverticillate and biverticillate conidiophores, while those of T. francoae are biverticillate. Both novel endophytes are illustrated and described.
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- 2021
405. Drying kinetics and quality characteristics of daylily dried by mid-infrared
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Shuaiyao Yang, Yifu Zhang, Ye Chen, Pei-yun Jiang, Nan Wang, Lan Jiang, and Xuechao Zheng
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Horticulture ,Materials science ,Kinetics ,Mid infrared ,Daylily ,Quality characteristics ,Engineering (miscellaneous) ,Food Science ,Biotechnology - Abstract
Industrially, the use of far-infrared (FIR) as a heat source for drying daylily presents some issues, such as high energy consumption and large loss of nutrients. The use of mid-infrared (MIR) was performed to study the drying of daylily to explore its advantages, with the FIR drying as a comparison. Drying models were established by the drying kinetics, and the changes of nutrition, rehydration ratio (RR) and water migration pattern were researched. The results showed the best-fitting drying model was the Modified Henderson and Pabis model. Under the same temperature, compared with FIR drying, the drying time of MIR drying was shortened by 50%, the effective moisture diffusivity (D eff) was increased by 103%, the drying activation energy (E a) was reduced by 10%, the reducing sugar and ascorbic acid retention rate was increased by 13.9% and 9.7%, respectively. The MIR drying had better RR and water migration characteristics.
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- 2021
406. Undergraduate nursing students' attitudes towards incarcerated persons and their perceptions of clinical experiences
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Jodi L. Sutherland, Patricia Reuther, Bing Si, Rosemary Collier, Deborah Palmer, and Lan Jiang
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Adult ,High rate ,Attitude of Health Personnel ,Undergraduate nursing ,Prisoners ,media_common.quotation_subject ,Stigma (botany) ,Correctional Facilities ,Education, Nursing, Baccalaureate ,Cross-Sectional Studies ,Nursing ,Surveys and Questionnaires ,Scale (social sciences) ,Perception ,Humans ,Students, Nursing ,Baccalaureate nursing ,Psychology ,General Nursing ,Clinical learning ,media_common - Abstract
Background In 2019, an estimated 6.3 million persons were under the supervision of the United States adult correctional systems and one in 40 adult persons were under correctional supervision. At year-end of 2019, nearly seven in ten persons under correctional supervision were supervised in the community. A nurse's attitudes and beliefs about incarcerated persons may influence their behavior. Purpose The purpose of this study was to examine nursing student's attitudes towards incarcerated persons and their perceptions of a clinical experience at a correctional facility. Methods Data from 137 nursing students were collected through a cross-sectional, electronic survey. The Attitude Towards Prisoners Scale (Melvin et al., 1985) was used. A post hoc analysis of perceptions of their clinical experience at a correctional facility was explored. Results The mean scores of 93 and 94 (potential range of scores 0 to 144) suggest positive attitudes towards incarcerated persons exist, yet few nursing students report an interest in correctional nursing as a career. Although few (n = 5) students had a correctional nursing clinical experience, most perceived it as an effective clinical learning setting. Conclusion With high rates of incarceration, the researchers recommend that baccalaureate nursing programs include educational teaching strategies that focus on incarceration, incarceration stigma, and explore clinical learning experiences.
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- 2021
407. Preparation of twin graphene quantum dots through the electric-field-assisted femtosecond laser ablation of graphene dispersions
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Xin Li, Misheng Liang, Yang Zhao, Le Ma, Xiaojie Li, Lan Jiang, Pei Zuo, Sumei Wang, and Xiaozhe Chen
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Materials science ,Photoluminescence ,business.industry ,Graphene ,Coulomb explosion ,Nanoparticle ,General Chemistry ,Laser ,law.invention ,law ,Quantum dot ,Electric field ,Femtosecond ,Optoelectronics ,General Materials Science ,business - Abstract
Twin graphene can introduce stable and extended defects to energy band engineering, giving unique electron transport properties, which is expected to have potential applications in the fields of magnetism, spin transport or photoluminescence. This study proposes a method of using electric-field-assisted temporally-shaped femtosecond laser ablation liquid (ETLAL) of graphene dispersion to prepare graphene quantum dots (GQDs) with an average particle size of 2–3 nm and oxygen-containing functional groups modified surface, which realizes the controllable preparation of single crystal and twin GQDs (5-fold twin). This method controls the crystallinity of GQDs from two aspects: (1) The intervention of an electric field can rapidly command the directional motion of the cavitation bubbles and the nanoparticles contained therein to collide and crystallize at higher temperatures and pressures, which is the key to the formation of twin GQD; (2) Adjusting temporally-shaped femtosecond laser pulse delay could control the proportion of the Coulomb explosion during the ablation process, which increases the carbon cluster supplied by the cavitation bubble which is the key of polyploid number in twin GQDs. This research provides a fast, green strategy for the preparation of unprecedented twin GQDs, which is of great significance to the applications in the field of 2D material defect engineering.
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- 2021
408. Polyvinylpyrrolidone-assisted aqueous synthesis of monodisperse CH3NH3PbBr3 perovskite nanocrystals
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Xiao-Lan Wei, Xiao-Di Hu, Lan Jiang, Qin-Ling Shi, and Si-Wei Wu
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Modeling and Simulation ,General Materials Science ,Bioengineering ,General Chemistry ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics - Published
- 2022
409. Unconventional Shrinkage of Hot Electron Distribution in Metal Directly Visualized by Ultrafast Imaging
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Guoquan Gao, Lan Jiang, Bofeng Xue, Fei Yang, Ti Wang, Yan Wan, and Tong Zhu
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General Materials Science ,General Chemistry - Abstract
Elucidation of hot carrier transport and cooling mechanisms at the micro-/nanoscale is critical for optoelectronics, thermal management, and photocatalysis. Spatiotemporal evolution of hot electrons is usually convoluted with their ultrafast dynamics. Herein, an ultrafast microscopy is employed to directly track the spatiotemporal distribution of photoexcited hot electrons, providing a transformative approach to unravel the competitive relationship of transport and cooling. In the temporal evolution profiles of hot electron distribution, an anomalous contracting stage showing obvious thickness and fluence dependency is observed, with a characteristic end time indicating the completion of electron-phonon (e-ph) thermalization. Hot electron transport plays a prominent role in the competition with e-ph coupling, while interfacial heat dissipation dominates nonequilibrium state evolution with thickness below ballistic length. This work significantly enriches the tool kit of ultrafast techniques and provides guidance for rational design and optimization of micro-/nanodevices.
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- 2022
410. Screening for broad-spectrum antimicrobial endophytes from Rosa roxburghii and multi-omic analyses of biosynthetic capacity
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Hong Zhang, Mao-Fa Yang, Qian Zhang, Bin Yan, and Yu-Lan Jiang
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Plant Science - Abstract
Plants with certain medicinal values are a good source for isolating function-specific endophytes. Rosa roxburghii Tratt. has been reported to be a botanical source of antimicrobial compounds, which may represent a promising candidate for screening endophytic fungi with antimicrobial potential. In this study, 54 endophytes were isolated and molecularly identified from R. roxburghii. The preliminary screening using the plate confrontation method resulted in 15 different endophytic strains showing at least one strong inhibition or three or more moderate inhibition against the 12 tested strains. Further re-screening experiments based on the disc diffusion method demonstrated that Epicoccum latusicollum HGUP191049 and Setophoma terrestris HGUP190028 had excellent antagonistic activity. The minimum inhibitory concentration (MIC) test for extracellular metabolites finally indicated that HGUP191049 had lower MIC values and a broader antimicrobial spectrum, compared to HGUP190028. Genomic, non-target metabolomic, and comparative genomic studies were performed to understand the biosynthetic capacity of the screened-out endophytic fungus. Genome sequencing and annotation of HGUP191049 revealed a size of 33.24 megabase pairs (Mbp), with 24 biosynthetic gene clusters (BGCs), where the putative antimicrobial compounds, oxyjavanicin, patulin and squalestatin S1 were encoded by three different BGCs, respectively. In addition, the non-targeted metabolic results demonstrated that the strain contained approximately 120 antimicrobial secondary metabolites and was structurally diverse. Finally, comparative genomics revealed differences in pathogenicity, virulence, and carbohydrate-active enzymes in the genome of Epicoccum spp. Moreover, the results of the comparative analyses presumed that Epicoccum is a promising source of antimicrobial terpenes, while oxyjavanicin and squalestatin S1 are antimicrobial compounds shared by the genus. In conclusion, R. roxburghii and the endophytic HGUP191049 isolated from it are promising sources of broad-spectrum antimicrobial agents.
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- 2022
411. Case report: Clinical and genetic analysis of a family with nonsyndromic auditory neuropathy
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Lan Jiang, Hongen Xu, Danhua Liu, Sen Zhang, and Ying Xu
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Pediatrics, Perinatology and Child Health - Abstract
BackgroundAuditory neuropathy (AN) is a hearing disorder caused by the failure of inner hair cells, auditory nerve synapses and/or auditory nerves. With the development of high-throughput sequencing technology, the genetic factors of AN have been revealed, and genetic testing has become an important tool for identifying different types of AN.Case descriptionTo study the genetic cause of nonsyndromic auditory neuropathy in a Chinese family. The family was from Henan Province with three affected individuals. The audiological examinations were performed on the affected individuals, and whole-exome sequencing was carried out on the proband. The suspected pathogenic variants screened by the bioinformatic analysis were validated using Sanger sequencing in the family members. We identified three novel variants c.3277G > A (p.Glu1093Lys), c.4024-4G > T, and c.898-2A > G of the OTOF gene in the three children with AN. The first two variants were inherited from their father, and the third variant was inherited from their mother. A minigene assay was designed to test the effect of c.4024-4G > T on splicing. The variants c.3277G > A, c.4024-4G > T, and c.898-2A > G could be classified as likely pathogenic/pathogenic following the ACMG guidelines, and they are considered as the genetic causes for the patients in the family.ConclusionNew pathogenic/likely pathogenic variants of the OTOF gene were identified in a family with AN, enriching the mutational spectrum of the OTOF gene.
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- 2022
412. Ficolin-2: A potential immune-related therapeutic target with low expression in liver cancer
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Li-Ting, Wang, Qiu-Ling, Zeng, Shao-Lan, Jiang, Zhen-Yu, Chen, Xiao-Ling, Wang, Ling, Li, and Xiaolong, Li
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Cancer Research ,Oncology - Abstract
ObjectiveThis study aimed to investigate the role of ficolin-2 (FCN2) in the development and course of hepatocellular carcinoma (HCC) and to contribute to the evolution of innovative HCC therapeutics.MethodsOncomine, GEPIA (Gene Expression Profiling Interactive Analysis), TISIDB (Tumor Immune System Interactions and Drug Bank database), UALCAN (University of Alabama at Birmingham Cancer data analysis portal), UCSC (University of California, Santa Cruz), R package, the Kaplan–Meier technique, Cox regression analysis, LinkedOmics, Pearson’s correlation, and a nomogram were used to investigate the prognostic value of FCN2 in HCC. Co-expressed genes were screened. A protein–protein interaction network was created using the STRING database. Finally, immunohistochemistry was performed to establish the expression of FCN2 in HCC tissues. A pan-cancer study centered on HCC-related molecular analysis was also conducted to look for a link between FCN2 and immune infiltration, immune modulators, and chemokine receptors.ResultsIn HCC tissues, the expression of FCN2 was observed to be lower than that in normal tissues. This was connected to the HCC marker alpha-fetoprotein, showing that FCN2 is involved in the development and progression of cancer. FCN2 may act through Staphylococcus aureus infection, lectins, and other pathways. Furthermore, at the immune level, the expression of FCN2 in HCC was associated with some immune cell infiltration, immunomodulators, and chemokine receptors.ConclusionFCN2 may be an immune checkpoint inhibitor for HCC, creating a breakthrough in the treatment of HCC.
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- 2022
413. Bayesian Tracking and Parameter Learning for Non-Linear Multiple Target Tracking Models.
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Lan Jiang 0002, Sumeetpal S. Singh, and Sinan Yildirim
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- 2015
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414. Calibrating the Gaussian multi-target tracking model.
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Sinan Yildirim, Lan Jiang 0002, Sumeetpal S. Singh, and Thomas Dean
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- 2015
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415. A Monte Carlo expectation maximisation algorithm for multiple target tracking.
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Sinan Yildirim, Lan Jiang 0002, Sumeetpal S. Singh, and Thomas Dean
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- 2012
416. One-Step Fabrication Method of MoS2 for High-Performance Surface-Enhanced Raman Scattering
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Wenpan Tao, Lan Jiang, Jingya Sun, Feifei Wang, Jichao Song, Changji Pan, and Qingsong Wang
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Fabrication ,Materials science ,business.industry ,Physics::Optics ,One-Step ,Substrate (electronics) ,Laser ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Pulse (physics) ,law.invention ,symbols.namesake ,General Energy ,law ,Femtosecond ,symbols ,Optoelectronics ,Physics::Atomic Physics ,Physical and Theoretical Chemistry ,Raman spectroscopy ,business ,Raman scattering - Abstract
A one-step method was proposed to prepare a high-performance surface-enhanced Raman scattering (SERS) substrate based on bulk MoS2 via femtosecond laser pulse modification. The Raman spectra of Rho...
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- 2021
417. A novel sparse model-based algorithm to cluster categorical data for improved health screening and public health promotion
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Lan Jiang, Yu Ding, Melissa A. Sutherland, M. Katherine Hutchinson, Chuheng Zhang, and Bing Si
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Public Health, Environmental and Occupational Health ,Safety, Risk, Reliability and Quality ,Safety Research - Published
- 2021
418. Typification of Sphaeria sinensis to precisely fix the application of the name of the economically important Chinese caterpillar fungus, Ophiocordyceps sinensis
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Yi Li, David L. Hawksworth, Lan Jiang, Yi-Jian Yao, Jiao-Jiao Lu, and Yong-Hui Wang
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Thitarodes ,biology ,Hirsutella ,Ophiocordyceps sinensis ,Plant Science ,Caterpillar fungus ,biology.organism_classification ,Ghost moth ,chemistry.chemical_compound ,chemistry ,Botany ,Medicinal fungi ,Typification ,Ecology, Evolution, Behavior and Systematics - Published
- 2021
419. Association of glycemic variability assessed by continuous glucose monitoring with subclinical diabetic polyneuropathy in type 2 diabetes patients
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Yinan Zhang, Lan Jiang, Congrong Wang, Jiemin Pan, Fengwen Li, and Xinfeng Yan
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Blood Glucose ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Type 2 diabetes ,Diseases of the endocrine glands. Clinical endocrinology ,Nerve conduction velocity ,Diabetic Neuropathies ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Humans ,Glycemic variability ,Risk factor ,Continuous glucose monitoring ,Subclinical infection ,Glycemic ,Glycated Hemoglobin ,business.industry ,Blood Glucose Self-Monitoring ,General Medicine ,RC648-665 ,medicine.disease ,Diabetic peripheral neuropathy ,Glucose ,Peripheral neuropathy ,Diabetes Mellitus, Type 2 ,Cardiology ,business ,Body mass index - Abstract
Aims/Introduction Diabetic peripheral neuropathy is a common diabetes‐related microvascular complication. The relationship between peripheral nerve function and glucose variability is unclear. We investigated the association of glucose variability with subclinical diabetic polyneuropathy in a large‐scale sample of patients with type 2 diabetes. Materials and Methods We enrolled 509 individuals with type 2 diabetes who were screened for diabetic peripheral neuropathy and monitored using a continuous glucose monitoring system. Multiple glycemic variability parameters, including the mean amplitude of glycemic excursions, glucose standard deviation (SDgluc) and glucose coefficient of variation were calculated from 3‐day glucose profiles obtained from continuous glucose monitoring. All participants underwent nerve conduction studies, and the composite Z‐scores for nerve conduction parameters were calculated. Results Multivariate logistic regression analyses showed that SDgluc and the conventional risk factor hemoglobin A1c (HbA1c) were independently associated with abnormal nerve function, and the corresponding odds ratios (95% confidence interval) were 1.198 (1.027–1.397, SDgluc) and 1.182 (1.061–1.316, HbA1c), respectively. The composite Z‐score of nerve conduction velocity and response amplitude obviously decreased with greater SDgluc, and the composite Z‐score of distal latency significantly increased with increasing tertiles of SDgluc (all P trend
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- 2021
420. Effects of novel flavonoid-enriched yogurt on the diversity of intestinal microbiota in mice
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Weijun Wang, Daodong Pan, Zhen Wu, Xiaoqun Zeng, Lan Jiang, Qiang Xia, and Xiefei Li
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Firmicutes ,Flavonoid ,Gut flora ,Microbiology ,Butyric acid ,Feces ,Mice ,chemistry.chemical_compound ,Media Technology ,Animals ,Cellulases ,Food microbiology ,Glycosides ,Food science ,Flavonoids ,chemistry.chemical_classification ,Mice, Inbred ICR ,biology ,Probiotics ,fungi ,food and beverages ,Bacteroidetes ,Biodiversity ,Yogurt ,biology.organism_classification ,Gastrointestinal Microbiome ,Aglycone ,chemistry ,Food Microbiology - Research Paper ,Fermentation - Abstract
Soy isoflavone glycoside cannot be effectively absorbed by the human intestinal tract, but probiotics with related hydrolases can transform it into aglycone to promote its absorption. In this study, a novel flavonoid-enriched yogurt was developed using an isolated β-glucosidase-producing strain (Lactiplantibacillus plantarum GY). The flavonoid aglycone-enhanced yogurt was fed to ICR mice for 21 days, and its effects were observed. The yogurt can affect the gut microbial diversity of mice, especially increasing the abundance of Parasutterella, the Bacteroidales S24-7 group, and Phascolarctobacterium in the intestinal tract of mice. Meanwhile, the ratio of Bacteroidetes/Firmicutes in the intestinal tract of mice fed with the flavonoid aglycone-enriched yogurt increased. The difference in the content of butyric acid between the L-GY + IS and the control groups was significant (P
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- 2021
421. Critical Role of Simultaneous Reduction of Atmospheric Odd Oxygen for Winter Haze Mitigation
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Yuanhang Zhang, Xin Sun, Jay G. Slowik, Qiao Zhu, Qiao-Li Zou, Kaspar R. Daellenbach, Ling-Yan He, Li-Ming Cao, Keding Lu, André S. H. Prévôt, Yong Cheng, Ye-Min Shen, Li-Liang Lin, Eri Saikawa, Xiao-Feng Huang, Nga L. Ng, Bing-Ye Xu, Min Hu, Lan Jiang, Xu-Dong Tian, Yuhan Liu, and Qian Tang
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China ,Haze ,Ozone ,Coronavirus disease 2019 (COVID-19) ,Air pollution ,chemistry.chemical_element ,medicine.disease_cause ,Atmospheric sciences ,Oxygen ,chemistry.chemical_compound ,Air Pollution ,medicine ,Humans ,Environmental Chemistry ,Volatile organic compound ,Aerosols ,chemistry.chemical_classification ,Air Pollutants ,SARS-CoV-2 ,COVID-19 ,General Chemistry ,Particulates ,Aerosol ,chemistry ,Communicable Disease Control ,Environmental science ,Particulate Matter ,Environmental Monitoring - Abstract
The lockdown due to COVID-19 created a rare opportunity to examine the nonlinear responses of secondary aerosols, which are formed through atmospheric oxidation of gaseous precursors, to intensive precursor emission reductions. Based on unique observational data sets from six supersites in eastern China during 2019-2021, we found that the lockdown caused considerable decreases (32-61%) in different secondary aerosol components in the study region because of similar-degree precursor reductions. However, due to insufficient combustion-related volatile organic compound (VOC) reduction, odd oxygen (Ox = O3 + NO2) concentration, an indicator of the extent of photochemical processing, showed little change and did not promote more decreases in secondary aerosols. We also found that the Chinese provinces and international cities that experienced reduced Ox during the lockdown usually gained a greater simultaneous PM2.5 decrease than other provinces and cities with an increased Ox. Therefore, we argue that strict VOC control in winter, which has been largely ignored so far, is critical in future policies to mitigate winter haze more efficiently by reducing Ox simultaneously.
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- 2021
422. Evaluating the Effect of Recurrent Respiratory Papillomatosis on Work Productivity
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Amanda Hu, Thomas K. Chung, Tanya K Meyer, Lan Jiang, Maya G. Sardesai, and Haley Wilcox
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Adult ,Male ,Human papilloma virus ,medicine.medical_specialty ,Work productivity ,Voice Disorders ,Papilloma ,Voice Quality ,business.industry ,Papillomavirus Infections ,General Medicine ,Middle Aged ,Indirect costs ,Otorhinolaryngology ,Work (electrical) ,Presenteeism ,Humans ,Medicine ,Female ,Recurrent Respiratory Papillomatosis ,business ,Intensive care medicine ,Respiratory Tract Infections ,Work Performance - Abstract
Objective: Patients with recurrent respiratory papillomatosis (RRP) have significant vocal dysfunction which affects their performance at work. This study aimed to evaluate voice-related work productivity before and after ablative treatment for RRP. Methods: This is a prospective case series conducted at 2 academic laryngology outpatient clinics. Adult employed patients with RRP completed the Work Productivity & Activity Impairment instrument (WPAI), Voice Handicap Index (VHI-10), WorkHoarse, Hospital Anxiety and Depression Scale (HADS), and a demographics questionnaire immediately before and 1 month after ablative treatment of papilloma. The primary outcome measure was the change in work productivity impairment domain of the WPAI, and changes in ratings before and after ablation were compared using a Wilcoxon Signed-Rank test. Results: The 32 participants (mean age 45, 84% male) had a median (interquartile range) voice-related work productivity impairment score of 48.8% (30.0) at baseline which was improved to 5.0% (10.0) at 1 month after surgical ablation of papillomata (difference 30.0% (30.0) improvement). For the secondary outcome measures, there were significant improvements in VHI-10 ( P Conclusion: Patients with RRP experience significant voice-related work productivity impairment, and ablation of papillomata significantly improves work productivity.
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- 2021
423. To purchase or to remove? Online shopping cart warning pop-up messages can polarize liking and purchase intention
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Xuehua Wang, Haichuan Zhao, and Lan Jiang
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Marketing ,Cart ,media_common.quotation_subject ,05 social sciences ,ComputerApplications_COMPUTERSINOTHERSYSTEMS ,Regret ,Advertising ,Scarcity ,Extant taxon ,0502 economics and business ,Message type ,050211 marketing ,Business ,050203 business & management ,media_common - Abstract
Online companies, such as eBay and Taobao, often set limits on the maximum number of items placed in the shopping cart. When the number of items added exceeds the limit, a warning message would pop up, asking consumers to clean, remove, or purchase first. Extant research on online shopping cart abandonment examines the various factors that can lead to shopping cart abandonment but does not explore the effect of such warning messages on attitudes and purchase intention. Through five studies (i.e., Studies 1, 2A, 2B, 3, and 4), we show that reminding consumers to clean items in their online shopping carts can polarize liking and purchase intention towards the most-favorite and least-favorite items. Furthermore, we find that reminding consumers to remove items (rather than to purchase them) magnifies the polarization. Consumers’ anticipated regret mediates the interactive effect of warning message type and preference ranking on liking and purchase intention. In addition, the scarcity or abundance of product supply moderates the interaction effect of shopping cart warning message type and preference ranking on liking and purchase intention. Implications for both theories and practices are discussed.
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- 2021
424. Leptin receptor antagonist attenuates experimental autoimmune thyroiditis in mice by regulating Treg/Th17 cell differentiation
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Wei Wang, Bo-Tao Zhang, Qi-Lan Jiang, Han-Qing Zhao, Qin Xu, Yang Zeng, Jia-Ying Xu, and Jun Jiang
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Leptin ,Mice ,Endocrinology, Diabetes and Metabolism ,Thyroiditis, Autoimmune ,Animals ,Th17 Cells ,Receptors, Leptin ,Cell Differentiation ,Hashimoto Disease ,T-Lymphocytes, Regulatory ,Autoimmune Diseases - Abstract
Leptin has been found to be involved in the development and progression of many autoimmune diseases. As an organ-specific autoimmune disease, the pathogenesis of Hashimoto’s thyroiditis has not been fully elucidated. It has been reported that serum leptin level is increased in Hashimoto’s thyroiditis, but other studies have not shown any difference. We replicated a mouse model of experimental autoimmune thyroiditis (EAT) with a high-iodine diet and found that injection of the leptin receptor antagonist Allo-aca reduced thyroid follicle destruction and inflammatory cell infiltration in EAT mice, and thyroxine and thyroid autoimmune antibody levels. Further investigation revealed that Allo-aca promotes the differentiation of Treg cells and inhibits the differentiation of Th17 cells. We believe that Allo-aca can alter the differentiation of Treg/Th17 cells by inhibiting the leptin signaling pathway, thereby alleviating thyroid injury in EAT mice. Interfering with the leptin signaling pathway may be a novel new approach to treat treating and ameliorating Hashimoto’s thyroiditis.
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- 2022
425. Test and Optimization of Oilseed Rape (Brassica napus L.) Threshing Device Based on DEM
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Jun Wu, Qing Tang, Senlin Mu, Lan Jiang, and Zhichao Hu
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Plant Science ,oilseed rape (Brassica napus L.) ,threshing concave plate ,discrete element method ,design ,experiment ,Agronomy and Crop Science ,Food Science - Abstract
Gridded concave plate sieves are usually used for threshing operations of grain and oilseed crops. In response to the problems of high threshing loss rate and grain breakage rate when threshing oilseed rape, this research modified the threshing concave plate of oilseed rape (Brassica napus L.) harvesters to improve the performance and efficiency of oilseed rape separation. The improved threshing concave plate adopts a 360° wrap angle, and a guide plate with an adjustable inflow angle is designed on the inner side of the concave plate. The optimal combination of parameters for the threshing device is determined by simulation analysis and field testing. Single-factor simulations of the threshing cylinder speed and guide plate angle are carried out using EDEM, which showed that both are influencing factors for the force and speed of the oilseed rape particles. A three-factor and three-level orthogonal experiment was undertaken to validate the simulation analysis further. The threshing cylinder speed, concave plate speed, and guide plate angle were influencing factors. The threshing loss rate and grain breakage rate are evaluation indicators. The field validation tests are carried out on concave plates with 180° wrap angle and 360° wrap angle, the results showed that the concave plate with 360° wrap angle reduces the threshing loss rate by 4.25%, the grain breakage rate by 0.93%, and improved the harvesting efficiency by 0.31 km/h when the threshing cylinder speed was 81.89 rad/s, concave plate speed was 9.34 rad/s, and guide plate angle was 40°. This study demonstrates that the concave plate with a 360° wrap angle has better performance and operational efficiency, and it provides design ideas for threshing devices for other crop combine harvesters.
- Published
- 2022
- Full Text
- View/download PDF
426. Design and Test of Seedbed Preparation Machine before Transplanting of Rapeseed Combined Transplanter
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Lan Jiang, Qing Tang, Jun Wu, Wenyi Yu, Min Zhang, Dong Jiang, and Dexin Wei
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Plant Science ,agricultural machinery ,seedbed preparation machine ,rapeseed ,transplanting ,test ,parameter optimization ,Agronomy and Crop Science ,Food Science - Abstract
In order to satisfy the soil preparation requirements of a rapeseed combined transplanter in the middle-lower Yangtze River region in China where rice–rapeseed rotation cropping system was performed, a seedbed preparation machine composed of a rotary tillage device, ditch cleaning shovel, and soil leveling auger was designed to realize the function of rotary tillage, stubble ploughing, ditching, and soil leveling. The seedbed preparation machine was designed as the two parts of the middle section and the left–right symmetrical section to realize the need for middle ditching. Based on the principle of active scraping and anti-blocking, the curves of the soil contact section, soil throwing section, and transition section of the ditch cleaning shovel were analyzed. The structure parameters of the soil leveling auger with end reversal structure were designed. In order to further improve the working performance of the seedbed preparation machine, the response surface tests were designed, selecting the forward speed(X1), the rotation speed of rotary tillage blade roller(X2), and the rotation speed of soil leveling auger(X3) as the main influencing factors, taking the soil breaking rate and the straw coverage rate and the soil flatness as the test indexes. The optimal parameter combination was obtained as a forward speed of 0.94 m/s, rotation speed of rotary blade roller of 268 rpm, and rotation speed of soil leveling auger of 204 rpm. Under the optimal parameters combination, the soil breaking rate, straw coverage rate, and soil flatness were 92.06%, 93.01%, and 8.35 mm respectively, which satisfied the agronomic requirements of rapeseed blanket seedling transplanting. This study can provide a reference for the design of a seedbed preparation machine of a rapeseed combined transplanter.
- Published
- 2022
- Full Text
- View/download PDF
427. Design and Test of the Seedling Cavitation and Lodging Monitoring System for the Rape Blanket Seedling Transplanter
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Min Zhang, Zhan Jiang, Lan Jiang, Chongyou Wu, and Yao Yang
- Subjects
Plant Science ,Agronomy and Crop Science ,rape blanket seedling ,lodging ,seedling cavitation ,transplanter ,picture processing ,Food Science - Abstract
To realize the real-time monitoring of the lodging and cavitation state of the rape blanket seedling transplanter, a real-time online monitoring system set for cavitation and lodging based on machine vision was designed. The system was mainly composed of an image acquisition module, a height sensor, and an IPC (Industrial Personal Computer). When the system starts to work, the height of the lens from the ground and the collected images are saved in real time, and the operating status of the equipment is judged according to the height of the lens from the ground, and the actual corresponding size of the image pixels is corrected. We proposed a calculation method for judging the cavitation and lodging state of the seedlings based on the comparison between the actual planting position and the theoretical planting position of the seedlings. The processing and analysis software for the cavitation and lodging monitoring system of the rape blanket transplanter was compiled, and the monitoring system was developed. The dynamic test showed that the maximum absolute error of the cavitation recognition rate was 1.23%, and the maximum absolute error of the lodging recognition rate was 3.80%. The total time consumed of a 1 m image collected in real time from the collection, image processing and stitching, to seedling status recognition of the rape blanket seedling field was about 1 s. The accuracy and system processing time of the seedling cavitation and lodging state judgment can meet the accuracy and real-time requirements of cavitation and lodging state recognition for field transfer of rape blanket seedlings.
- Published
- 2022
- Full Text
- View/download PDF
428. Transcatheter ventricular septal defect closure via femoral vein alone under transthoracic echocardiography guidance without fluoroscopy: preliminary experience
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LiuLiu Huang, Mai Chen, Decai Zeng, Chun Xiao Su, Chun Lan Jiang, Bao Shi Zheng, and Ji Wu
- Abstract
Objective. Transcatheter closure of congenital ventricular septal defects under echocardiography guidance could avoid potential radiation damage from fluoroscopy probes. However, mini-surgical incision, transesophageal echocardiography combined with tracheal intubation, and limited artery access further complicate the procedure. Therefore, we aimed to describe our preliminary experiences in percutaneous VSD closure via the femoral vein only under transthoracic echocardiography (TTE) guidance. Methods. Between December 2018 and November 2021, 19 patients underwent transcatheter VSD closure via femoral vein alone under the guidance of TTE in our hospital. The morphology, location, diameter of VSD, and procedural outcomes were thoroughly reviewed. Symmetric, asymmetric, or eccentric occlusion device was chosen for closure based on the VSD characteristics. Results. There were 16 perimembranous VSD and 3 intracristal VSD patients in this cohort. The range of diameter of the VSD was from 3.8 to 7.4 mm. Fifteen symmetrical occluders were implanted in 13 perimembranous and 2 intracristal VSD patients. Two eccentric occluders were implanted in 1 perimembranous and 1 intracristal VSD patient since the rim to the aortic valve distance was Conclusions. Transcatheter perimembranous and intracristal VSD closure via femoral vein alone under the TTE guidance is feasible and safe in eligible patients. The new type of multipurpose specialized catheter will facilitate this procedure.
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- 2022
429. Comparing Resident, Proxy, and Staff Respondents for Nursing Home Residents' Preferences for Everyday Living
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Zachary J. Kunicki, Caroline Madrigal, Lien T. Quach, Melissa R. Riester, Lan Jiang, Matthew S. Duprey, Melanie Bozzay, Andrew R. Zullo, Mriganka Singh, John McGeary, Wen-Chih Wu, and James L. Rudolph
- Subjects
Patient-Centered Care ,Humans ,Homes for the Aged ,Geriatrics and Gerontology ,Medicare ,Gerontology ,United States ,Proxy ,Aged ,Nursing Homes - Abstract
To encourage person-centered care, the Centers for Medicare and Medicaid require nursing homes to measure resident preferences using the Preferences Assessment Tool (PAT). No known research has examined the implications of respondent type (i.e., resident, proxy, staff) on preference importance; therefore, the purpose of this study was to compare the importance of preferences depending on which respondent completed the PAT. Participants included 16,111 Veterans discharged to community-based skilled nursing facilities after hospitalization for heart failure. A majority (95%) of residents completed the PAT compared to proxy (3%) and staff (2%). Proxy responders were both more and less likely to indicate individual preferences as important compared to residents. Staff members were consistently less likely to indicate all preferences as important compared to residents. Findings from this study emphasize the need for proxy and staff to find methods to better understand residents’ preferences when residents are not able to participate in assessments.
- Published
- 2022
430. Femtosecond Laser-Induced Anisotropic Structure and Nonlinear Optical Response of Yttria-Stabilized Zirconia Single Crystals with Different Planes
- Author
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Ziqian Ning, Yiling Lian, Lan Jiang, Jingya Sun, Shouyu Wu, and Feifei Wang
- Subjects
General Materials Science - Abstract
Nonlinear optical properties have been extensively studied due to their promising nonlinear effects and various applications. With ultrashort duration and ultrahigh intensity, a femtosecond laser can fabricate various superior-quality micro-/nanostructures to improve the nonlinearity of materials, which are promising for stable and high-performance nonlinear devices. In this contribution, yttria-stabilized zirconia (YSZ) with fs laser-induced micro-/nanostructures is demonstrated to exhibit unique anisotropic light-material interaction and nonlinear optical response on [100], [110], and [111] planes. Time-resolved reflectivity of YSZ after fs laser excitation is investigated by a pump-probe experiment, which is consistent with simulations through the plasma model combined with a two-temperature model. These results indicate two early ablation mechanisms: Coulomb explosion and melting. Anisotropic crack structures are formed due to thermal stress, which is always ignored in fs laser fabrication and is verified by Raman mapping and analysis of slip systems on different crystal planes. Through the
- Published
- 2022
431. Femtosecond Laser Bessel Beam Fabrication of a Supercapacitor with a Nanoscale Electrode Gap for High Specific Volumetric Capacitance
- Author
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Heng Guo, Jianfeng Yan, Lan Jiang, Shengfa Deng, Xinzhu Lin, and Liangti Qu
- Subjects
General Materials Science - Abstract
Supercapacitors are widely used in electronic systems as energy storage devices. The fabrication of a miniaturized supercapacitor with high specific capacitance has attracted much attention in recent years. Here, we propose a new method to fabricate supercapacitors with a nanoscale electrode gap by using a femtosecond laser. The original femtosecond laser was converted to a nondiffraction Bessel light field with nanoscale beam width and microscale focal depth. Nanoscale processing precision was achieved by regulating the Bessel beam. We fabricated graphene supercapacitors with different electrode gap widths (varying from the microscale to the nanoscale) using this method. Supercapacitors fabricated by this method have advantages in both size miniaturization (electrode gap width down to ∼500 nm) and electrochemical performance improvement (a specific volumetric capacitance of 195 F/cm
- Published
- 2022
432. Sparse-View CBCT Reconstruction Using Combined DRUNet and HQS
- Author
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Lan Jiang, Xiaoyi Wang, Hesong Jiang, Xueyuan Wang, Haiyan Guo, and Xiaochun He
- Published
- 2022
433. Human-Induced-Vibration Response Analysis and Comfort Evaluation Method of Large-Span Steel Vierendeel Sandwich Plate
- Author
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Lan Jiang, Ruoheng Cheng, Huagang Zhang, and Kejian Ma
- Subjects
Vierendeel sandwich plate ,comfort evaluation ,human-induced load ,acceleration response distribution ,Architecture ,Building and Construction ,Civil and Structural Engineering - Abstract
A steel Vierendeel sandwich plate used as a large-span lightweight floor structure for vibration comfort during crowd gatherings was considered. Taking the steel Vierendeel sandwich plate in Guizhou Museum as an example, through finite element transient analysis, the effects of the structural damping, pedestrian self-weight, floor span, surface concrete slab thickness, and structural parameters on the floor’s acceleration response distribution were deeply studied. According to the distribution characteristics of the acceleration response, a distribution model function was constructed, and a distribution Gauss model of the relationship between the peak acceleration response and the position of the steel Vierendeel sandwich plate was established. A field test of the sandwich plate under human-induced fixed-point excitation was carried out, and the model fitting results were compared with the actual test results. The results showed that the Gaussian model could effectively estimate the peak acceleration response at different positions on the floor. In addition, according to the distribution model, a comfort evaluation method based on the comfort assurance rate was proposed that could greatly reduce the representative value of the acceleration evaluation. The research results provide a reference for the comfort evaluation and corresponding vibration-reduction design of long-span steel Vierendeel sandwich plates.
- Published
- 2022
- Full Text
- View/download PDF
434. Superelastic graphene aerogel-based metamaterials
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Mingmao Wu, Hongya Geng, Yajie Hu, Hongyun Ma, Ce Yang, Hongwu Chen, Yeye Wen, Huhu Cheng, Chun Li, Feng Liu, Lan Jiang, and Liangti Qu
- Subjects
Multidisciplinary ,General Physics and Astronomy ,General Chemistry ,General Biochemistry, Genetics and Molecular Biology - Abstract
Ultralight, ultrastrong, and supertough graphene aerogel metamaterials combining with multi-functionalities are promising for future military and domestic applications. However, the unsatisfactory mechanical performances and lack of the multiscale structural regulation still impede the development of graphene aerogels. Herein, we demonstrate a laser-engraving strategy toward graphene meta-aerogels (GmAs) with unusual characters. As the prerequisite, the nanofiber-reinforced networks convert the graphene walls’ deformation from the microscopic buckling to the bulk deformation during the compression process, ensuring the highly elastic, robust, and stiff nature. Accordingly, laser-engraving enables arbitrary regulation on the macro-configurations of GmAs with rich geometries and appealing characteristics such as large stretchability of 5400% reversible elongation, ultralight specific weight as small as 0.1 mg cm−3, and ultrawide Poisson’s ratio range from −0.95 to 1.64. Additionally, incorporating specific components into the pre-designed meta-structures could further achieve diversified functionalities.
- Published
- 2022
435. Chemical control over the conversion between bicyclic and polycyclic terpenes by fungal bifunctional terpene synthases
- Author
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Xinye Wang, Zhixin Wang, Guoliang Zhu, Lan Jiang, Weiyan Zhang, Yiyi Huang, Zhanren Cong, Yi-Lei Zhao, Jian-He Xu, Tom Hsiang, Lixin Zhang, Qi Chen, and Xueting Liu
- Subjects
Alkyl and Aryl Transferases ,Sesterterpenes ,Terpenes ,Materials Chemistry ,Metals and Alloys ,Ceramics and Composites ,General Chemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Abstract
Fungal bifunctional terpene synthases (BFTSs) reportedly associate with a series of new skeletons of di/sesterterpenes. However, the molecular mechanisms underlying the variabilities in the ring system of BFTS-catalyzed products are not well understood. In this study, we identified a key site, S89/L89, that controls the conversion between bicyclic and polycyclic terpene skeletons catalyzed by two BFTSs, BsPS and FoFS. Our analysis revealed that a mutation on site 89 in the BFTSs alters the carbocation transportation pathway and redirects the competing reactions for previously unreported terpenes.
- Published
- 2022
436. Impact of Various Thermistors on the Properties of Resistive Microbolometers Fabricated by CMOS Process
- Author
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Guo, Yaozu, primary, Ma, Haolan, additional, Lan, Jiang, additional, Liao, Yiming, additional, and Ji, Xiaoli, additional
- Published
- 2022
- Full Text
- View/download PDF
437. Research on Airline Pilot Allocation Based on Safety Considerations
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Lei, Liu, primary, Mingxiao, Du, additional, and Lan, Jiang, additional
- Published
- 2022
- Full Text
- View/download PDF
438. Influence of CrFeCoNiCu high-entropy alloy and ultrasonic stirring on the thermal, electrochemical and mechanical properties of Zn-30Sn high-temperature solder alloy
- Author
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Bin Chen, Minming Zou, Wenjing Chen, Huajin Zhang, Jue Wang, Lan Jiang, Zezong Zhang, Jiatao Zhou, Xiaowu Hu, Qinglin Li, and Xiongxin Jiang
- Subjects
Mechanics of Materials ,Mechanical Engineering ,General Materials Science ,Condensed Matter Physics - Published
- 2023
439. Banking market structure and industrial structure: A transnational empirical study
- Author
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Lan Jiang and Tong Wu
- Subjects
Finance - Published
- 2023
440. Heterojunction engineering between 2D MoSe2 nanosheets and 1D Ta3N5 nanofibers for boosted photocatalytic hydrogen production
- Author
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Xiaoqiang Zhan, Huilin Hou, Deliu Ou, Haitao Zhang, Bing Li, Lan Jiang, Fengmei Gao, Leyao Xu, and Weiyou Yang
- Subjects
Fuel Technology ,Nuclear Energy and Engineering ,Renewable Energy, Sustainability and the Environment ,Materials Science (miscellaneous) ,Energy Engineering and Power Technology - Published
- 2023
441. Structure, stability, and mechanism of dextran–CPP–Ca2+ conjugates: A novel high-efficiency calcium ion delivery system
- Author
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Shuhong Li, Siqi Qiang, Jiake Wang, Tongliang Yang, Lan Jiang, Yifu Zhang, and Ye Chen
- Subjects
General Medicine ,Food Science ,Analytical Chemistry - Published
- 2023
442. Analysis of predictions considering mineral prices, residential energy, and environmental risk: Evidence from the USA in COP 26 perspective
- Author
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Lan Jiang and Hua Jiang
- Subjects
Economics and Econometrics ,Sociology and Political Science ,Management, Monitoring, Policy and Law ,Law - Published
- 2023
443. An all-in-one magnetic SERS nanosensor for ratiometric detection of Escherichia coli in foods
- Author
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Lan Jiang, Xiao-Di Hu, Qin-Ling Shi, Yi-Wei Weng, and Xiao-Lan Wei
- Subjects
Silver ,Aptamer ,Metal Nanoparticles ,Nanoparticle ,Spectrum Analysis, Raman ,medicine.disease_cause ,Benzoates ,Ferric Compounds ,Biochemistry ,Analytical Chemistry ,symbols.namesake ,Limit of Detection ,Nanosensor ,Escherichia coli ,medicine ,Animals ,Humans ,Sulfhydryl Compounds ,Escherichia coli Infections ,Detection limit ,Chromatography ,Chemistry ,Substrate (chemistry) ,Milk ,Manganese Compounds ,Food Microbiology ,symbols ,Raman spectroscopy ,Food Analysis ,Raman scattering - Abstract
An all-in-one nanosensor was developed for the magnetic enrichment and ratiometric surface-enhanced Raman scattering (SERS) detection of Escherichia coli (E. coli). The all-in-one nanosensor was constructed through the chemical integration of four components into a single nanoparticle, which include a manganese ferrite nanoparticle serving as the magnetic core, a thin silver shell as the SERS substrate, a self-assembled layer of 4-mercaptobenzoic acid (MBA) molecules as the SERS internal standard, and a MBA-conjugated layer of aptamer sequences as the capture probe of E. coli. In the detection of E. coli in food, the target cells were first captured by the nanosensors and magnetically enriched in a short time of 15 min, and then the ratiometric SERS was performed through the Raman intensity ratio between two specific SERS peaks produced by the captured E. coli and the internal MBA. The pre-concentration and ratiometry enabled the nanosensor to detect E. coli with a detection limit down to 10 CFU/mL. The all-in-one nanosensor was successfully applied for the detection of E. coli in various liquid foods including milk, juice, tea, and coffee, with recoveries ranging from 89 to 110% and relative standard deviation lower than 1.7%. In comparison with the previous sandwich strategy adopted by most SERS sensors, this nanosensor endowed with an easier use and a lower cost is more sensitive and reproducible, leading to a great potential in practical applications.
- Published
- 2021
444. Genome-guided investigation of anti-inflammatory sesterterpenoids with 5-15 trans-fused ring system from phytopathogenic fungi
- Author
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Xueting Liu, Ronald J. Quinn, Jianying Han, Loganathan Karthik, Zhixin Wang, Tom Hsiang, Xuyuan Wang, Jie Zhang, Liya Zhao, Zhanren Cong, Lan Jiang, Huanqin Dai, Biao Ren, Weize Yuan, Chengjian Hou, Xiangyang Liu, Gao-Yi Tan, Lei Ma, Lixin Zhang, Xue Zhang, Xuekui Xia, Xiangyin Chen, Guang Liu, Xinye Wang, Huanting Yang, Mei Liu, Guoliang Zhu, Shu-Shan Gao, and Kangjie Lv
- Subjects
0303 health sciences ,Bicyclic molecule ,biology ,030306 microbiology ,General Medicine ,Applied Microbiology and Biotechnology ,Genome ,Terpenoid ,Terpene ,03 medical and health sciences ,Biochemistry ,Glycosyltransferase ,biology.protein ,Heterologous expression ,Gene ,030304 developmental biology ,Biotechnology ,Genomic organization - Abstract
Fungal terpenoids catalyzed by bifunctional terpene synthases (BFTSs) possess interesting bioactive and chemical properties. In this study, an integrated approach of genome mining, heterologous expression, and in vitro enzymatic activity assay was used, and these identified a unique BFTS sub-clade critical to the formation of a 5-15 trans-fused bicyclic sesterterpene preterpestacin I (1). The 5-15 bicyclic BFTS gene clusters were highly conserved but showed relatively wide phylogenetic distribution across several species of the diverged fungal classes Dothideomycetes and Sordariomycetes. Further genomic organization analysis of these homologous biosynthetic gene clusters from this clade revealed a glycosyltransferase from the graminaceous pathogen Bipolaris sorokiniana isolate BS11134, which was absent in other 5-15 bicyclic BFTS gene clusters. Targeted isolation guided by BFTS gene deletion led to the identification of two new sesterterpenoids (4, and 6) from BS11134. Compounds 2 and 4 showed moderate effects on LPS-induced nitrous oxide production in the murine macrophage-like cell line RAW264.7 with in vitro inhibition rates of 36.6 ± 2.4% and 24.9 ± 2.1% at 10 μM, respectively. The plausible biosynthetic pathway of these identified compounds was proposed as well. This work revealed that phytopathogenic fungi can serve as important sources of active terpenoids via systematic analysis of the genomic organization of BFTS biosynthetic gene clusters, their phylogenetic distribution in fungi, and cyclization properties of their metabolic products. KEY POINTS: • Genome mining of the first BFTS BGC harboring a glycosyltransferase. • Gene-deletion guided isolation revealed three novel 5-15 bicyclic sesterterpenoids. • Biosynthetic pathway of isolated sesterterpenoids was proposed.
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- 2021
445. Mechanism of Liquid Dispersion Enhancement by the Hydrophobic Wire Mesh at Macro- and Micro-Scale
- Author
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Jing-Peng Zhang, Guo-Yu Ren, Yong Luo, Hai-Long Liao, Guang-Wen Chu, Lan Jiang, and Jiang-Nan Li
- Subjects
Mechanism (engineering) ,Materials science ,Scale (ratio) ,Wire mesh ,General Chemical Engineering ,Dispersion (optics) ,General Chemistry ,Composite material ,Macro ,Industrial and Manufacturing Engineering - Published
- 2021
446. Tough hydrogels with rapid self-reinforcement
- Author
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Hideaki Yokoyama, Chang Liu, Takako Noritomi, Kohzo Ito, Lan Jiang, Sohei Kawahara, Koichi Mayumi, and Naoya Morimoto
- Subjects
Toughness ,Multidisciplinary ,Materials science ,Polyethylene glycol ,law.invention ,Crystallinity ,chemistry.chemical_compound ,chemistry ,law ,Self-healing hydrogels ,Crystallization ,Composite material ,Elongation ,Reinforcement ,Order of magnitude - Abstract
Tougher when stressed Hydrogels are typically weak materials because they contain only a small fraction of polymeric material highly swollen with water. Strength can be increased by adding extra cross-linking or interpenetrating networks with some sacrificial bonds to enhance toughness; however, these properties deteriorate upon stretching and may be slow to recover after relaxation. Liu et al. developed polyethylene glycol hydrogels cross-linked with moderate fractions of polymers that form sliding rings. These allow the chain to orient in parallel when stretched, leading to rapid and reversible strain-induced crystallization and thus much tougher hydrogels. Science , aaz6694, this issue p. 1078
- Published
- 2021
447. Genome-Based Discovery of Enantiomeric Pentacyclic Sesterterpenes Catalyzed by Fungal Bifunctional Terpene Synthases
- Author
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Lan Jiang, Yongheng Wang, Yuya Sato, Bin Zhu, Chengwei Liu, Xueting Liu, Hideaki Oikawa, Guoliang Zhu, Weiyan Zhang, Atsushi Minami, Zhixin Wang, Shu-Shan Gao, Tom Hsiang, Lixin Zhang, Jie Zhang, Xue Zhang, Taro Ozaki, Xinye Wang, and Kangjie Lv
- Subjects
Alkyl and Aryl Transferases ,Sesterterpenes ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Stereochemistry ,Organic Chemistry ,Fungi ,Stereoisomerism ,010402 general chemistry ,Ring (chemistry) ,01 natural sciences ,Biochemistry ,Genome ,Catalysis ,0104 chemical sciences ,Terpene ,chemistry.chemical_compound ,Cyclization ,Physical and Theoretical Chemistry ,Enantiomer ,Bifunctional - Abstract
Genome-based discovery of two previously unreported fungal bifunctional terpene synthases (BFTSs) from phytopathogenic fungi are reported: FoFS catalyzing the formation of fusoxypenes A-C (1-3) and (-)-astellatene (4) and AtAS capable of synthesizing preaspterpenacid I (6). Interestingly, FoFS and AtAS catalyzed the formation of enantiomeric sesterterpenes with a 5-6-7-3-5 ring system. C22-oxidative modification of preaspterpenacid I by AtP450 was characterized as well. Plausible cyclization pathways of the fusoxypenes were illustrated by DFT calculations.
- Published
- 2021
448. Effect of Acupuncture on Neuroplasticity of Stroke Patients with Motor Dysfunction: A Meta-Analysis of fMRI Studies
- Author
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Yingjia He, Linqing Miao, Xing Chen, Xiaodong Liu, Lan Jiang, Guixing Xu, Tongtong Liu, Yihuai Zou, Rong Zhang, Jie Chen, Qiuyi Lv, and Yuxin Pan
- Subjects
medicine.medical_specialty ,Acupuncture Therapy ,Neurosciences. Biological psychiatry. Neuropsychiatry ,Review Article ,Basal Ganglia ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Physical medicine and rehabilitation ,Neuroimaging ,Randomized controlled trial ,law ,Neuroplasticity ,Acupuncture ,Humans ,Medicine ,Insular Cortex ,Stroke ,030304 developmental biology ,0303 health sciences ,Movement Disorders ,Neuronal Plasticity ,business.industry ,medicine.disease ,Data Accuracy ,Treatment Outcome ,Neurology ,Meta-analysis ,Neurology (clinical) ,Motor Deficit ,business ,Insula ,030217 neurology & neurosurgery ,RC321-571 - Abstract
Importance. Acupuncture is an effective treatment for stroke, especially in the aspect of motor deficit. Many brain imaging studies of acupuncture have found significant changes in brain function after acupuncture treatment in order to reveal its underlying mechanisms in regulating neural plasticity. However, no definite consensus has been reached. Objective. To analyze the pattern of intrinsic brain activity variability that is altered by acupuncture compared with conventional treatment in stroke patients with motor dysfunction, thus providing the mechanism of stroke treatment by acupuncture. Methods. Chinese and English articles published up to May 2020 were searched in the PubMed, Web of Science, EMBASE, and Cochrane Library databases, China National Knowledge Infrastructure, Chongqing VIP, and Wanfang Database. We only included randomized controlled trials (RCTs) using resting-state fMRI to observe the effect of acupuncture on stroke patients with motor dysfunction. R software was used to analyze the continuous variables, and Seed-based d Mapping with Permutation of Subject Images (SDM-PSI) was used to perform an analysis of fMRI data. Findings. A total of 7 studies comprising 143 patients in the treatment group and 138 in the control group were included in the meta-analysis. The results suggest that acupuncture treatment helps the healing process of motor dysfunction in stroke patients and exhibits hyperactivation in the bilateral basal ganglia and insula and hypoactivation in motor-related areas (especially bilateral BA6 and left BA4). Conclusion. Acupuncture plays a role in promoting neuroplasticity in subcortical regions that are commonly affected by stroke and cortical motor areas that may compensate for motor deficits, which may provide a possible mechanism underlying the therapeutic effect of acupuncture.
- Published
- 2021
449. Antibacterial polyene-polyol macrolides and cyclic peptides from the marine-derived Streptomyces sp. MS110128
- Author
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Lan Jiang, Pei Huang, Guoliang Zhu, Zhijun Song, Wenni He, Lixin Zhang, Biao Ren, Huanqin Dai, Ayokunmi Oyeleye, Xueting Liu, and Jie Zhang
- Subjects
chemistry.chemical_classification ,0303 health sciences ,biology ,030306 microbiology ,Chemistry ,Stereochemistry ,General Medicine ,Bacillus subtilis ,Conjugated system ,biology.organism_classification ,Polyene ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Streptomyces ,Cyclic peptide ,03 medical and health sciences ,chemistry.chemical_compound ,Staphylococcus aureus ,medicine ,Antibacterial activity ,Candida albicans ,030304 developmental biology ,Biotechnology - Abstract
Marine microbes provide an important resource to discover new chemical compounds with biological activities beneficial to drug discovery. In our study, two new polyene macrolides, pyranpolyenolides A (1) and B (2), and one new natural cyclic peptide (9), together with two known polyenes (7 and 8) and three known cyclic peptides (10-12), were isolated from a culture of the marine Streptomyces sp. MS110128. In addition, four new polyene macrolides, pyranpolyenolides C-F (3-6), were identified as olefin geometric isomers that were most likely produced by photochemical conversion during the cultivation or isolation procedures. The pyranpolyenolides are 32-membered macrolides endowed with a conjugated tetraene and several pairs of 1,3-dihydroxyl groups. Pyranpolyenolides that contain a hydropyran group have not been previously reported. Four cyclic peptides (9-12) showed significant activities against Bacillus subtilis, Staphylococcus aureus, and methicillin-resistant S. aureus with supporting MIC values ranging from 0.025 to 1.25 μg/mL. These cyclic peptides containing piperazic moieties showed moderate activities with MIC values of 12.5 μg/mL against Bacille Calmette Guerin (BCG), an attenuated form of the bovine. Additionally, cyclic peptide 12 showed moderate antifungal activity against Candida albicans with an MIC value of 12.5 μg/mL. KEY POINTS: • Discovery of new polyenes and cyclic peptides from a marine-derived Actinomycete. • Cyclic peptides containing piperazic moieties exhibited good antibacterial activity.
- Published
- 2021
450. AFM-Based nanofabrication and quality inspection of three-dimensional nanotemplates for soft lithography
- Author
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Jingyan Dong, Huimin Zhou, Bing Si, Lan Jiang, Jia Deng, and Pual Cohen
- Subjects
0209 industrial biotechnology ,Materials science ,Polydimethylsiloxane ,Atomic force microscopy ,Strategy and Management ,Nanotechnology ,02 engineering and technology ,Management Science and Operations Research ,021001 nanoscience & nanotechnology ,Industrial and Manufacturing Engineering ,Soft lithography ,chemistry.chemical_compound ,020901 industrial engineering & automation ,Nanomanufacturing ,Nanolithography ,chemistry ,Casting (metalworking) ,Ultrasonic vibration ,Nanometre ,0210 nano-technology - Abstract
Soft lithography is one of the most promising nanofabrication technologies for scalable nanomanufacturing. It uses flexible molds or stamps to fabricate extremely high-resolution nanopatterns in a low cost and rapid fashion on both flat and curved surfaces. Although two-dimensional soft lithography has been widely investigated and applied for some applications, there is a lack of quantitative research on using nanometer scale 3D patterns fabricated by atomic force microscope (AFM) for 3D soft lithography. In this paper, we demonstrated the effectiveness of ultrasonic vibration assisted AFM-based nanomachining in fabricating 3D master nanotemplates for 3D soft lithography. We successfully applied Polydimethylsiloxane (PDMS) daughter molds, casted from a nanometer scale 3D master template, using a soft lithography technique solvent-assisted micromolding. In addition, we investigated the quality and reusability of the master nanotemplates, and the quality of the PDMS patterns by quantifying the volume differences using an automatic imaging registration algorithm. Results show that there are zero or little PDMS residuals in the polymethyl methacrylate (PMMA) master nanotemplate with a simple contour for the first and second daughter mold casting, but 7% residuals for the third casting. And a more complex master nanotemplate shows slightly higher residual levels.
- Published
- 2021
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