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Triazoles are the only compounds used as antibiotics in both medicine and agriculture. The presence of triazoles in the environment can contribute to the acquisition of azole resistance among isolates of Aspergillus fumigatus. The objective of this study was to investigate the effect of A.fumigatus exposure to triazoles on susceptibility to these compounds. Seventeen triazole-resistant and 21 triazole-sensitive A.fumigatus isolates were examined. The isolates were transferred 20 times on the Sabouraud medium supplemented with posaconazole, itraconazole or voriconazole, followed by five times on the medium not supplemented. The minimum inhibitory concentrations of antimycotics were examined according to the EUCAST broth microdilution method after the 20th transfer and also the 25th transfer. In addition, the expression levels of genes mdr1, mdr2, mdr3, atrF, cyp51A and cyp51B were determined. Cultivation of A. fumigatus on media supplemented with posaconazole, itraconazole and voriconazole resulted in the acquisition of resistance to the tested triazoles of all examined isolates. After recultivation on Sabouraud without azoles, most of the isolates lost their acquired resistance. The long-term use of triazole compounds in agriculture may result in the occurrence of triazole resistant A. fumigatus isolates in the environment, not only by induction or selection of mutations in the cyp51A gene, but also by contribution to changes in the gene expression. [ABSTRACT FROM AUTHOR]

Thousands of tons of pharmaceuticals are introduced into the aqueous environment due to their incomplete elimination during treatment process in wastewater treatment plants (WWTPs) and water treatment plants (WTPs). The presence of pharmacologically active compounds in the environment is of a great interest because of their potential to cause negative effects. Furthermore, drugs can undergo different processes leading to the formation of new transformation products, which may be more toxic than the parent compound. In light of these concerns, within the research a new, rapid and sensitive analytical procedure for the determination of a wide range of pharmaceuticals from different classes using solid phase extraction (SPE) and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) technique in different water samples was developed. This methodology was applied to investigate the occurrence, removal efficiency of 25 pharmaceuticals during wastewater and drinking water treatment, and seasonal variability in the amount of selected pharmaceuticals in WWTP and WTP over a year. The most often detected analytes in water samples were carbamazepine (100 % of samples) and ibuprofen (98 % of samples), concluding that they may be considered as pollution indicators of the aqueous environment in tested area. Highly polar compound, metformin, was determined at very high concentration level of up to 8100 ng/L in analyzed water samples. Drugs concentrations were much higher in winter season, especially for non-steroidal inflammatory drugs (NSAIDs) and caffeine, probably due to the inhibited degradation related to lower temperatures and limited sunlight. Carbamazepine was found to be the most resistant drug to environmental degradation and its concentrations were at similar levels during four seasons. [ABSTRACT FROM AUTHOR]

Effective public participation in decision-making concerning protected areas requires supportive legal provisions, practices and narratives. While there has been a wide interest in organisational aspects of participation in protected areas, discursive questions concerning the attitudes toward participation among stakeholder have received relatively little attention. Using Q methodology we investigated attitudes of 53 respondents, representing key stakeholder groups (local communities, NGOs, scientists, protected area staff, foresters, public officials, general public), toward the involvement of local communities in managing various forms of biodiversity conservation in Poland. We found three main discourses (1) positive toward participation and recognizing the conservation and development goals of protected areas; (2) sceptical toward participation and nature-centred; (3) cautiously open to participation and developmental goals of protected areas but highlighting organisational difficulties. There were diverse attitudes toward participation within stakeholder groups signalling potential for compromise among them. All three discourses opt for a mixed model of governance balancing central and local influence, which diverges from traditional centralized practices. They differ over barriers to participation, highlighting either insufficient capacity of administration or lack of knowledge and interests of local people. These differences indicate wider socio-political tensions that should be acknowledged during participation. [ABSTRACT FROM AUTHOR]

We explore the occurrence of the ecosystem services (ES) concept in public policies in Poland by providing a systematic content analysis of national environmental policies. A detailed analysis of the legal acts, national strategies, and decrees using the Common International Classification of Ecosystem Services codes, which embraces a full range of ES, shows that the ES concept is reflected in the investigated documents. However, it is mainly depicted in an indirect, latent form. We further explore the ES concept occurrence in the Polish legislation with in-depth interviews of experts. From the interviews we identify two general groups of barriers to the ES concept implementation in environmental policy: (a) a limited understanding and acknowledgement of the concept among individuals involved in policy making; and (b) sectoral divisions within environmental governance that hinder the spread of the concept. Analysis reveals that the concept of services for society provided by nature had already been perceived in Polish national environmental policies before the emergence of the ES concept and the implementation of the EU biodiversity policy. However, the concept is referred to mostly in a latent form, before and after its emergence. [ABSTRACT FROM AUTHOR]

The group of 18 new amide derivatives of mycophenolic acid (MPA) and selected heterocyclic amines was synthesised as potential immunosuppressive agents functioning as inosine-5'-monophosphate dehydrogenase (IMPDH) uncompetitive inhibitors. The synthesis of 14 of them employed uronium-type activating system (TBTU/HOBt/DIPEA) while 4 of them concerned phosphonic acid anhydride method (T3P/Py) facilitating amides to be obtained in moderate to excellent yields without the need of phenolic group protection. Most of optimised protocols did not require complicated reaction work-ups, including chromatographic, solvent-consuming methods. The biological activity assay was performed on the T-Jurkat cell line and peripheral mononuclear blood cells (PBMCs) which are both dedicated for antiproliferative activity determination. Each of designed derivatives was characterised by reduced cytotoxicity and benzoxazole analogue (A2) revealed the most promising activity. Subsequently, an observed structure-activity relationship was discussed. [ABSTRACT FROM AUTHOR]

• The modification with the PET did not inhibit the rheological propertied of the fresh mix, allowing for proper printing. • Incorporation of PET results in decreased compressive and flexural strengths regardless of the specimens (printed and casted, up to 48.9% and 51.9%, respectively). • The freeze–thaw cycles and high temperature exposure caused a significant deterioration of the mechanical properties of printed mixture. • 3D printed mortar with PET granules as the replacement of natural aggregate (10 vol-% to 50 vol-%). • The inclusion of PET can lead to decrease buildability of printed structure. 3D concrete printing technology of cementitious materials is challenging in many aspects. Despite the workability and mechanical properties of a mix and hardened concrete or mortar, the researchers need to face problems with environmental impact and durability of printed elements. Furthermore, today thousands of tons of waste are produced and natural aggregates which are most used resources by volume in the construction sector are on the verge of exhaustion. To date, limited knowledge about utilized of artificial aggregate (including Polyethylene terephthalate (PET) in 3D printed mortar is available. The main objective of this study is to develop 3D printed mortar with PET granules as the replacement of natural aggregate (10 vol-% to 50 vol-%). The paper contributes to knowledge of properties of 3D printed composites with plastic aggregate. Four printable mixes were made: one reference mix and three mixes in which natural aggregate was replaced by PET granulate in quantities amounting to 10%, 30% and 50% (by volume), respectively. The replacement ratios were chosen on the base of literature review for ordinary cementitious mortars. Several strength tests were carried out for standard and printed specimens. In addition, a freeze–thaw resistance test and high temperature performance test were conducted to evaluate to validate the properties of artificial aggregate. The results show that PET granulate is useful in 3D printing owing to their buildability and extrudability properties. Furthermore, printed mixes with high amount of PET (30% and 50%) granulate shows high decreases in strength (up to 75%). Unfortunately, after the freeze–thaw resistance test specimens with a high amount of PET granulate (30% and 50%) indicated high strength reduction (up to 80%). Exposure to a temperature exceeding the melting point of PET results in significant reduction of compressive strength for printed specimens (up to 68.8 % for 50 % PET addition). In addition, mixes with up to 10% PET can be used for most structural elements even under varying thermal conditions (even under extreme cold temperature). [ABSTRACT FROM AUTHOR]

• Paper describes a methodology that was successfully applied for the development of a temporary road traffic steel barrier in compliance with T1/W1 level according to EN 1317; • A simplified theoretical analysis of 178 barrier, laboratory test of three types of barrier connectors (for two types of barrier), explicit FEM modeling of four chosen barrier, and a full-scale crash test for final barrier are presented; • The stiffness of barrier connector was established based by laboratory barrier test (the FEM model was validated based on laboratory tests); • The chosen final barrier alternative passed the crash test for T1/W1 (TB21) containment level, performed by certified laboratory [35] , which proves that the presented procedure could be used successfully for traffic barrier development. Over a million people die in road crashes and tens of millions result injured or disabled each year, globally. Hence, there is a constant concern for improving the safety of the roads to protect road users, and those at the most risk, the road workers. Construction, maintenance, and utility work are constantly needed to ensure safety and functionality of roads and highways. Temporary road traffic barriers are used to separate passing traffic from the workspace and protect the workspace from an errant vehicle intrusion. Steel barriers are light, and their major advantage is rapid installation. These barriers are not anchored to the ground, and their behavior relies on the base-pavement friction, mass inertia, and stiffness of the system. The paper describes a methodology that was successfully applied for the development of a temporary road traffic steel barrier in compliance with the European specification EN 1317. The study included a simplified theoretical analysis of 178 barriers, static laboratory tests of three types of barrier connectors, development of explicit finite element models (EFEMs) of four selected barrier designs, and a full-scale crash test of the final barrier design. It is shown that static laboratory tests of complex connector systems can help to improve the design process and reduce the cost. The analysis and use of EFEMs was enough so that just a single full-scale crash test was sufficient for certification of the barrier design for use in Europe. The final barrier is compliant with the EN 1317–2 requirements for T1/W1 (TB21) containment level, which was confirmed by a full-scale crash test. [ABSTRACT FROM AUTHOR]

Border militarization can impede people's interactions with nature in borderlands. We surveyed one border community to understand how local use of Białowieża Forest, one of Europe's last primary forest complexes, is affected by militarization. Out of 100 returned surveys, most respondents had a negative view of enforced border security measures (closure of the border zone, construction of a border barrier, military activities). Many felt they were not adequately consulted about these policies. Respondents felt discouraged from visiting Białowieża Forest mainly due to the presence of uniformed services and worsened conditions of forest roads caused by heavy machinery and traffic. Newcomers reported negative impacts on their health and well-being, while people born in the region (locals) had mixed reactions. Although locals were more equivocal in opposing the border barrier than newcomers, both groups perceived the environmental costs of its construction as high and reported a sense of separation from neighboring Belarus., Competing Interests: Declarations. Conflict of interest: The authors have no competing interests to declare that are relevant to the content of this article., (© 2024. The Author(s) under exclusive licence to Royal Swedish Academy of Sciences.)

Hematopoietic stem cell transplant (HSCT) is the treatment of choice in various hematologic diseases, and kidney transplantation (KTx) is the best therapy for end-stage kidney disease. Chronic kidney disease (CKD) occurs relatively often after both types of transplantations. Anemia after both HSCT and KTx may be due to CKD and other reasons. This study aimed to assess the prevalence of anemia to CKD in 156 prevalent patients after HSCT and 80 after KTx. According to the World Health Organization's definition (hemoglobin <13 g/dL for men and <12 g/dL for women), the prevalence of anemia in the studied cohort after HSCT was 13% in women and 35% in men and for those after KTx, it was29% in men and 11%. Anemia in KTx was found in 46% of patients, whereas CKD was present in 53%. After HSCT, anemia was associated with CKD in 56% of women and 17% of men. In KTx, anemia and CKD was diagnosed in 21% of patients. Patients with anemia after KTx had significantly lower glomerular filtration rate (GFR), hemoglobin, and significantly higher creatinine levels. Age was related to the estimated GFR (eGFR; r = -0.39, P < .001) in patients who underwent HSCT and had anemia. In patients without anemia, age was negatively related to eGFR (r = -0.56, P < .001) and the hemoglobin-to-platelet count (r = 0.62, P < .001). In KTx, hemoglobin was related to eGFR (r = 0.35, P < .001), and age was related to eGFR (r = -0.20, P < .05). The type of induction therapy immunosuppressive regimen (anti-thymocyte globulin vs basiliximab vs no induction) did not affect the prevalence of anemia in the KTx population studied. Anemia is relatively common in CKD after HSCT. In both CKD and coexistent anemia, nephrology referral is to be considered to optimize therapy, including nephroprotection., Competing Interests: Declaration of competing interest All the authors declare no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

Hematopoietic stem cell transplantation could be complicated by acute kidney injury and chronic kidney disease. It may be due to either previous chemotherapy or exposure to a variety of nephrotoxic drug or other causes. The aim of the study was to assess biomarkers of kidney injury in patients at least 3 months after hematopoetic stem cell transplantation (HSCT) under ambulatory care of the Hematology, Transplantation and Internal Medicine Department. We studied 80 prevalent patients after allogeneic HSCT and 32 healthy volunteers to obtain normal ranges of biomarkers. In this cross-sectional study we assessed retinol-binding protein 4 (RBP4), a biomarker of kidney injury in urine using commercially available assays. It was significantly higher in patients after HSCT when compared to healthy volunteers. When we divided patients according to kidney function (below and over 60 mL/min/1.72 m 2 ), we found that the concentration of RBP4 was significantly higher in 23 patients with chronic kidney disease stage 3 compared to patients with estimated glomerular filtration (eGFR) over 60 mL/min/1.72 m 2 . In univariate correlations RBP4 was positively related to serum creatinine (r = 0.34, P < .01) and inversely to eGFR (r = -0.20, P < .05). Patients after allogeneic HSCT despite normal or near normal kidney function show evidence of kidney injury., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)

The hematopoietic stem cell transplantation (HSCT) is performed for various hematological diseases. Chronic kidney disease (CKD) occurs relatively often after HSCT. Anemia after HSCT may be due to CKD and/or other reasons. The aim of this study is to assess the prevalence of anemia and its possible relationship to the presence of CKD in patients at least 3 months after HSCT. The study included 156 patients who underwent allogeneic HSCT treatment in our center in the years 1998 to 2021 due to different hematologic pathologies (acute myeloid leukemia, acute lymphoblastic leukemia, lymphoma, and others). Anemia was diagnosed in 13% of women and 35% of men. Anemia was most common in people after HSCT due to a history of acute myeloid leukemia (55% women, 30% men). In 56% of women and 17% of men, anemia was associated with chronic kidney disease. In patients with anemia, age was related to the eGFR (r = -0.39, p  < 0.001), in patients without anemia age was negatively related to eGFR (r = -0.56, p  < 0.001), and hemoglobin was positively related to platelet count ( r  = 0.62, p  < 0.001). Concluding, anemia, was relatively common in CKD after HSCT. In CKD, in particular with coexistent anemia, nephrology referral is to be taken into account to optimize therapy, including nephroprotection.

Translocation of abdominal organs into the thoracic cavity may cause dyspnea, heart disorders, and gastric symptoms. Diaphragmatic hernias can cause diagnostic difficulties, since both clinical and radiological symptoms might imitate different disorders. In these cases computed tomography of the chest is the method of choice. The aim of this study was to assess clinical manifestations, risk factors, and prognosis in patients with huge diaphragmatic hernias with displacement of abdominal organs into the thorax, depending on the action taken. We carried out a retrospective study using data of patients hospitalized in the years 2012-2016. Ten patients were qualified for the study (8 women and 2 men). The mean age of the subjects was 86.5 ± 10.5 years. Thirty percent of the hernias were post-traumatic. All of the patients reported cardiovascular or respiratory symptoms. Upper gastrointestinal symptoms occurred in half of the patients. Twenty percent of patients underwent surgery with a positive outcome, while 30% of patients, who were not qualified for surgery due to numerous co-morbidities, died. The main risk factors predisposing to the occurrence of large diaphragmatic hernias were the following: old age, female gender, and thoracic cage deformities.

Von Meyenburg complexes is one of the polycystic liver diseases, characterized by bile duct hamartoma. These cysts come from the biliary tract but the cysts do not communicate with them. Because of asymptomatic course of the lesions usually are diagnosed in the course of diagnostic for another reason. It is not possible to define the entire diagnosis based upon ultrasonography imaging, as cyst could mimic metastasis, micro-abscesses and multiple focal nodular lesions. Because of the small size of the lesion (0.5-15 mm) usually inconclusive is also computed tomography. On the basis of magnetic resonance imaging (MRI) and cholangio-MRI we can determine the diagnosis of the complexes. Liver biopsy is obligatory in case of suspicion of neoplastic process. These complexes do not require treatment, but long-term follow-up is indicated because of the possibility to more frequent cholangiocarcinoma in patient with von Meyenburg complexes. It is probably the first case report of the von Meyenburg complexes described in Poland.