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Key message In vitro embryo development is highly plastic; embryo cell fate can be re-established in tissue culture through different pathways. Abstract In most angiosperms, embryo development from the single-celled zygote follows a defined pattern of cell divisions in which apical (embryo proper) and basal (root and suspensor) cell fates are established within the first cell divisions. By contrast, embryos that are induced in vitro in the absence of fertilization show a less regular initial cell division pattern yet develop into histodifferentiated embryos that can be converted into seedlings. We used the Brassica napus microspore embryogenesis system, in which the male gametophyte is reprogrammed in vitro to form haploid embryos, to identify the developmental fates of the different types of embryogenic structures found in culture. Using time-lapse imaging of LEAFY COTYLEDON1-expressing cells, we show that embryogenic cell clusters with very different morphologies are able to form haploid embryos. The timing of surrounding pollen wall (exine) rupture is a major determinant of cell fate in these clusters, with early exine rupture leading to the formation of suspensor-bearing embryos and late rupture to suspensorless embryos. In addition, we show that embryogenic callus, which develops into suspensor-bearing embryos, initially expresses transcripts associated with both basal- and apical-embryo cell fates, suggesting that these two cell fates are fixed later in development. This study reveals the inherent plasticity of in vitro embryo development and identifies new pathways by which embryo cell fate can be established.

Intertidal salt marshes provide a range of valuable ecosystem services which typically increase with marsh width. Understanding the drivers for salt marsh expansion versus retreat is thus key to managers. Previous research highlights the influence of short-term (daily/event) bed level dynamics on germination and establishment and subsequent vegetation presence. However, more recent literature suggests the importance of medium-term seasonal bed level dynamics on viable seed availability and subsequent vegetation presence. This study aims to assess event-based and seasonal bed level dynamics for vegetation presence in natural and semi-natural salt marshes and to provide generic thresholds for vegetation presence. To gain insight into bed level dynamics, data was used from autonomous Optical and Acoustic Surface Elevation Dynamics sensors (O-SED and A-SED) around the edge of natural and semi-natural salt marshes. Sensors were installed at vegetated and unvegetated measurement station Field observations from 22 O-SEDs deployed at 4 well-established natural salt marshes in the Western Scheldt estuary and 4 O-SEDs at a well-established semi-natural salt marsh in the Wadden Sea were reanalyzed. Six novel A-SEDs were deployed at a pioneer semi-natural salt marsh in the Ems-Dollard Estuary. The measurement duration at all salt marshes was at least 1 year. The A-SED sensor was successfully validated against manual measurements. Furthermore, vegetation data and water level data were obtained. No significant difference was observed between natural and semi-natural salt marshes. However, a significant difference between vegetated and unvegetated measurement stations for short-term bed level dynamics was observed. Vegetation was found to be present at locations restricted by short-term bed level variability smaller than or equal to 12 mm, emphasizing the presence of a short-term threshold. Although trends in the non-growing season were significantly different between vegetated and unvegetated stations, seasonal thresholds for vegetation presence were not found. The findings imply that knowledge of bed level-dynamics in well-established natural marshes can be used to predict vegetation presence in constructed semi-natural marshes. The importance of local short-term dynamics for vegetation presence instead of longer-term dynamics highlights possibilities for developing favorable conditions for vegetation presence in marsh restoration projects and the construction of new salt marsh ecosystems.

Community Based Participatory Research (CBPR) provides important insights in how to mobilize a community for health promotion. This article explores the possibilities for shifting the frame from community to publics for building new forms of public health engagement in disadvantaged neighbourhoods. We present the results of an ethnographic research project on health and resilience in three low-income neighbourhoods in Maastricht, the Netherlands. In the context of a broader University-Citizens collaboration, citizens and researchers organized a monthly philosophy café to discuss subjects related to health and wellbeing. We analyse this in terms of public building and argue that shifting the perspective from community to publics strengthens the emancipatory tradition within CBPR. By creating an open setting, accommodating diversity and disagreements between its participants, the philosophy café constituted a local epistemic public, generating knowledge on health and wellbeing in disadvantaged neighbourhoods.In this article, we present an analysis of how researchers and citizens in low-income neighbourhoods collaborated to improve health and resilience. Citizens and researchers together organized a monthly philosophy café to discuss subjects related to health and wellbeing. We analyse the philosophy café as the activities of a public rather than a community. The philosophy café was set up as an open setting in which strangers could meet and exchange ideas and experiences. This resulted in lively exchanges and disagreements on what a good life entails. By presenting the philosophy café as a local public, our paper stresses the desire of neighbourhood inhabitants to meet strangers and exchange ideas, rather than to form close attachments to a neighbourhood community.

Integrated care models can expand access to behavioral health services, but provider payment is a significant barrier to integration.

It is well-known that the postprandial muscle protein synthetic response to protein ingestion is regulated on various levels, including dietary protein digestion and amino acid (AA) absorption, splanchnic AA retention, the availability of dietary protein–derived AA in the circulation, delivery of AA to the muscle, uptake of AA by the muscle, and intramuscular signaling. AA availability after consumption of dairy products is primarily determined by the rate of gastric emptying of milk proteins, which is mainly linked to coagulation of milk proteins in the stomach. Caseins form gastric coagula, which make their gastric emptying and subsequent postprandial aminoacidemia notably slower than that of whey proteins. Only recently, the role of processing, food structure, preservation and matrix on coagulation herein has been getting attention. In this review we describe various processes, that affect gastric coagulation of caseins and therewith control gastric emptying, such as the conversion to caseinate, heat treatment in the presence of whey proteins, conversion to stirred yoghurt and enzymatic hydrolysis. Modulating product characteristics by processing can be very useful to steer the gastric behavior of protein, and the subsequent digestion and AA absorption and muscle anabolic response to maintain or increase muscle mass.

The BABY BOOM (BBM) AINTEGUMENTA-LIKE (AIL) AP2/ERF domain transcription factor is a major regulator of plant cell totipotency, as it induces asexual embryo formation when ectopically expressed. Surprisingly, only limited information is available on the role of BBM during zygotic embryogenesis. Here we reexamined BBM expression and function in the model plant Arabidopsis thaliana ( Arabidopsis ) using reporter analysis and newly developed CRISPR mutants. BBM was expressed in the embryo from the zygote stage and also in the maternal (nucellus) and filial (endosperm) seed tissues. Analysis of CRISPR mutant alleles for BBM ( bbm-cr ) and the redundantly acting AIL gene PLETHORA2 ( PLT2 ) ( plt2-cr ) uncovered individual roles for these genes in the timing of embryo progression. We also identified redundant roles for BBM and PLT2 in endosperm proliferation and cellularization and the maintenance of zygotic embryo development. Finally, we show that ectopic BBM expression in the egg cell of Arabidopsis and the dicot crops Brassica napus and Solanum lycopersicon is sufficient to bypass the fertilization requirement for embryo development. Together these results highlight roles for BBM and PLT2 in seed development and demonstrate the utility of BBM genes for engineering asexual embryo development in dicot species.

CMMI payment and delivery reform models are testing approaches to support beneficiaries with social, economic, and location-based needs that drive health.

Research in Attention-deficit/hyperactivity disorder (ADHD) has had a clear focus on treatment and the dysfunction in specific situation associated with the condition. However, self-report, observational and anecdotal evidence indicates that there are also positive aspects associated with ADHD. Research on the potential positive features in individuals with an ADHD diagnosis is still limited, especially studies with larger representative samples. Here we performed qualitative research to identify positive aspects and strengths associated with ADHD in a large convenience sample from the Dutch organization for people with ADHD, dyslexia and dyscalculia. We sent out open-ended questionnaires to the members of the organization, asking what they consider to be positive aspects of their ADHD. From the responses of individuals with ADHD (n = 206), we extracted 116 codes, which were assigned to thirteen subthemes, which in turn led to five themes. These themes were: Creativity, Being dynamic, Flexibility, Socio-affective skills, and Higher-order cognitive skills. Core symptoms of ADHD such as impulsivity and hyperactivity were also considered positive aspects of ADHD by a minority of participants. After showing our results to a group of additional individuals with ADHD (focus group) they confirmed the identified positive aspects of ADHD. They also helped us with the interpretation of our findings and mentioned certain positive aspects to be a consequence of living with ADHD (being open minded and being honest). In conclusion, experiencing positive aspects seems to be common in ADHD as almost all participants reported positive aspect of ADHD, these aspects cover many different domains. Awareness about ADHD's strengths might help individuals with ADHD and their environment to better cope with, accept or embrace their diagnosis and for example make educational or occupational choices that fit them well. To incorporate these positive aspects in the understanding of ADHD, future research should focus on quantifying strengths in ADHD, and on investigating the link between these aspects and clinical characteristics and how this new knowledge can be implemented in psychoeducation and find its way to education and occupational settings.

Despite several effective medicines for treating opioid addiction, tight regulation in the U.S. prevents many suffering from addition from receiving them.

In this paper, new large-scale experimental data are presented showing the beach profile evolution, sediment transport and intra-swash hydrodynamics of two different beach slopes (1:15 and 1:25). In these experiments an erosive bichromatic wave series with groups of 4 waves (equivalent to random time series with Hs=0.65 m and Tp=3.5s) were produced in consecutive runs with each wave group repeating every two group periods (TR=2Tg=28.6s) in order to produce distinct types of swash interactions. Results show that the swash at the 1:15 slope developed a concave morphology with offshore directed transport, as typically seen for erosive conditions. Conversely, the swash at the 1:25 slope developed a beach step with overall onshore-directed transport typical of an accretive beach, even though the surf zone showed characteristics of an erosive regime. The differences in the developing morphologies are associated with the differences in wave-swash interactions observed at the different slopes. Following the observed intra-swash hydrodynamics, we hypothesize that the stronger bores on the steeper slope result in shorter swash periods, leading to variations of the swash interactions’ cross-shore location and to offshore-directed transport in the lower and middle swash. On the gentle slope, the backwash vortexes that generate the beach step stir up the sediment that is transported and deposited onshore by the following longer up-rushes. Further analysis of intra-swash sediment concentrations, both suspended and in the sheet flow layer, and hydrodynamics will be done to investigate the slope effect on the sediment transport processes.

Magnetic nanoparticles (MNPs) are widely used to facilitate magnetic particle imaging (MPI) which has the potential to become the leading diagnostic instrument for biomedical imaging. This comparative study assesses the effects of changing iron content and excitation frequency on point-spread function (PSF) representing the effect of magnetization reversal. PSF is quantified by features of interest for MPI: i.e. gradient amplitude and full-width-at-half-maximum (FWHM). A superparamagnetic quantifier (SPaQ) is used to assess differential magnetic susceptibility of two commercially available MNPs: Synomag��-D50 and Synomag��-D70. For both MNPs, the signal output depends on increase in drive field frequency and amount of iron-oxide, which might be hampering the sensitivity of MPI systems that perform on higher frequencies. Nevertheless, there is a clear potential of Synomag��-D for a stable MPI resolution, especially in case of 70 nm version, that is independent of either drive field frequency or amount of iron-oxide., International Journal on Magnetic Particle Imaging, Vol 8 No 1 Suppl 1 (2022)

Integral Field Spectrographs (IFS) often require non-trivial calibration techniques to process raw data. The OH Suppressing InfraRed Imaging Spectrograph at the W. M. Keck Observatory is a lenslet-based IFS that requires precise methods to associate the flux on the detector with both a wavelength and a position on the detector. During calibration scans, a single column lenslet mask is utilized to keep light from adjacent lenslet columns separate from the primary lenslet column, in order to uniquely determine spectral response of individual lenslets on the detector. Despite employing a single column lenslet mask, an issue associated with such calibration schemes may occur when light from adjacent masked lenslet columns leaks into the primary lenslet column. Incorrectly characterizing the flux due to additional light in the primary lenslet column results in one form of crosstalk between lenslet columns, which most clearly manifest as non-physical artifacts in the spectral dimension of the reduced data. We treat the problem of potentially blended calibration scans as a source separation problem and implement Non-negative Matrix Factorization (NMF) as a way to separate blended calibration scan spectra. After applying NMF to calibration scan data, extracted spectra from calibration scans show reduced crosstalk of up to 26.7% ± 0.5% while not adversely impacting the signal-to-noise ratio. Additionally, we determined the optimal number of calibration scans per lenslet column needed to create NMF factors, finding that greatest reduction crosstalk occurs when NMF factors are created using one calibration scan per lenslet column.

Photobiomodulation (PBM) utilises light energy to treat oral disease, periodontitis. However, there remains inconsistency in the reporting of treatment parameters and a lack of knowledge as to how PBM elicits its molecular effects in vitro. Therefore, this study aimed to establish the potential immunomodulatory effects of blue and near infra-red light irradiation on gingival fibroblasts (GFs), a key cell involved in the pathogenesis of periodontitis. GFs were seeded in 96-well plates in media + / − Escherichia coli lipopolysaccharide (LPS 1 μg/ml), or heat-killed Fusobacterium nucleatum (F. nucleatum, 100:1MOI) or Porphyromonas gingivalis (P. gingivalis, 500:1MOI). Cultures were incubated overnight and subsequently irradiated using a bespoke radiometrically calibrated LED array (400–830 nm, irradiance: 24 mW/cm2 dose: 5.76 J/cm2). Effects of PBM on mitochondrial activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and adenosine triphosphate (ATP) assays, total reactive oxygen species production (ROS assay) and pro-inflammatory/cytokine response (interleukin-8 (IL-8) and tumour growth factor-β1 (TGFβ1)) were assessed 24 h post-irradiation. Data were analysed using one-way ANOVA followed by the Tukey test. Irradiation of untreated (no inflammatory stimulus) cultures at 400 nm induced 15%, 27% and 13% increases in MTT, ROS and IL-8 levels, respectively (p P. gingivalis, F. nucleatum or LPS induced significant decreases in TGFβ1 secretion relative to their bacterially stimulated controls (p P. gingivalis, 400 nm irradiation induced 14% increases in MTT, respectively, relative to bacteria-stimulated controls (p