401. Generation and characterization of a single-chain Fv antibody against G, a hedgehog signaling pathway transcription factor.
- Author
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Laht S, Meerits K, Altroff H, Faust H, Tsaney R, Kogerman P, Järvekülg L, Paalme V, Valkna A, and Timmusk S
- Subjects
- Amino Acid Sequence, Animals, COS Cells, Cell Nucleus metabolism, Chlorocebus aethiops, Cytoplasm metabolism, Humans, Mice, Molecular Sequence Data, Signal Transduction, Transcription Factors chemistry, Zinc Finger Protein Gli3, Hedgehog Proteins metabolism, Hybridomas metabolism, Immunoglobulin Variable Region chemistry, Kruppel-Like Transcription Factors metabolism, Nerve Tissue Proteins metabolism, Transcription Factors metabolism
- Abstract
Gli3 is a key regulator of development, controlling multiple patterning steps. Here we report the generation of a scFv antibody specific to the repressor domain of human Gli3. We show that this scFv retains the binding capacity of its parent anti-Gli3 monoclonal antibody derived from hybridoma clone 5E1. When expressed in mammalian cells, the anti-Gli3 scFv co-localizes with intracellular Gli3. Immunocytochemical staining of the intrabody in Gli3-positive TM4 cells shows a distinct perinuclear cytoplasmic localization. Such a scFv constitutes a useful tool for studying transcriptional regulation of the hedgehog pathway in mammals and offers a starting point for developing novel Gli-related therapeutic intrabodies.
- Published
- 2008
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